Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,050 C→A 6.1% G34C (GGC→TGC)  rplN ← 50S ribosomal subunit protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,0500CA 6.1% 412.2 / 13.6 115G34C (GGC→TGC) rplN50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base C (51/57);  new base A (4/3);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 118 total aligned reads displayed.

GCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447902‑3448198
                                                                                                                                                    |                                                                                                                                                    
gCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGcc                                                                                                                                                      >  3:561607/1‑149 (MQ=255)
gCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGcc                                                                                                                                                      >  3:326326/1‑149 (MQ=255)
        aTCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGc                                                                                                                                              >  1:342959/1‑149 (MQ=255)
         tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCg                                                                                                                                             >  1:292964/1‑149 (MQ=255)
           gAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGCATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGAc                                                                                                                                           >  4:64794/1‑149 (MQ=255)
           gAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGAc                                                                                                                                           >  2:227101/1‑149 (MQ=255)
           gAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACACGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcgac                                                                                                                                          <  4:736948/149‑6 (MQ=255)
            aaGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACg                                                                                                                                          >  2:434983/1‑149 (MQ=255)
             aGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         <  1:245164/149‑1 (MQ=255)
             aGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         >  1:403078/1‑149 (MQ=255)
                gAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGc                                                                                                                                      >  3:750827/1‑149 (MQ=255)
                  aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:438879/149‑1 (MQ=255)
                       aGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAc                                                                                                                               >  4:552336/1‑149 (MQ=255)
                                  ggCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTg                                                                                                                    >  1:556081/1‑149 (MQ=255)
                                   gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTTATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  1:770262/149‑1 (MQ=255)
                                   gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  4:796298/149‑1 (MQ=255)
                                     gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 <  1:665301/149‑1 (MQ=255)
                                        gaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  2:567435/1‑127 (MQ=255)
                                        gaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcga                                                                                                                                           >  1:502424/1‑115 (MQ=255)
                                        gaACACCCTTATTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTCCCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcga                                                                                                                                           <  2:502424/119‑5 (MQ=255)
                                         aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATAcaca                                                                                                             <  1:485519/149‑1 (MQ=255)
                                         aaCACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATAcaca                                                                                                             <  1:637906/149‑1 (MQ=255)
                                          acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACAt                                                                                                            >  2:777222/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  2:719836/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  2:817261/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  1:659770/1‑149 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                             <  2:12481/112‑3 (MQ=255)
                                              ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                             >  1:12481/1‑110 (MQ=255)
                                               cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgc                                                                                                       >  2:740133/1‑149 (MQ=255)
                                                ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  1:17478/1‑119 (MQ=255)
                                                   ttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACg                                                                                                   >  4:448598/1‑149 (MQ=255)
                                                           caccacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCAACCCAGAACCTTGATACACATTACGCGACGTGCACCgg                                                                                           >  3:197365/1‑149 (MQ=255)
                                                            accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  2:266063/149‑1 (MQ=255)
                                                            accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  3:590782/149‑1 (MQ=255)
                                                              cacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGt                                                                                        >  3:9951/1‑149 (MQ=255)
                                                               acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       <  3:124569/149‑1 (MQ=255)
                                                               acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       >  3:438662/1‑149 (MQ=255)
                                                                         tCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACg                                                                             <  2:556081/149‑1 (MQ=255)
                                                                             cacaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         <  4:750827/149‑1 (MQ=255)
                                                                             cacaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         >  2:258157/1‑149 (MQ=255)
                                                                                  cACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     >  4:474342/1‑100 (MQ=255)
                                                                                  cACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     <  3:474342/100‑1 (MQ=255)
                                                                                   aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGCGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAg                                                                   >  4:352393/1‑149 (MQ=255)
                                                                                    ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        >  1:456924/1‑95 (MQ=255)
                                                                                    ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        <  2:456924/95‑1 (MQ=255)
                                                                                    ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGt                                                                  <  2:892/149‑1 (MQ=255)
                                                                                     ctcttttGACCTTACCACTCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATCCACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTc                                                                 >  4:60573/4‑149 (MQ=255)
                                                                                     cTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTc                                                                          <  1:63081/140‑1 (MQ=255)
                                                                                     cTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTc                                                                          >  2:63081/1‑140 (MQ=255)
                                                                                      ttttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCt                                                                >  1:528171/1‑149 (MQ=255)
                                                                                       tttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTg                                                               <  1:227101/149‑1 (MQ=255)
                                                                                        ttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGt                                                              <  3:384961/149‑1 (MQ=255)
                                                                                            gACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          >  1:778482/1‑149 (MQ=255)
                                                                                             aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    >  1:277804/1‑138 (MQ=255)
                                                                                               cTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  1:683907/1‑149 (MQ=255)
                                                                                               cTTACCACGCGGAACTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  3:59479/1‑149 (MQ=255)
                                                                                                    cACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGAGACGGTGCGAGCCAACCAGAACCTTGATACACATTACGCGACGTGCTCCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCAtt                                                  <  2:564399/149‑1 (MQ=255)
                                                                                                     aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTa                                                                                                          >  2:320783/1‑92 (MQ=255)
                                                                                                     aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTa                                                                                                          <  1:320783/92‑1 (MQ=255)
                                                                                                     aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCAttt                                                 <  3:150179/149‑1 (MQ=255)
                                                                                                       gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  1:817261/149‑1 (MQ=255)
                                                                                                       gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  3:105549/149‑1 (MQ=255)
                                                                                                       gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  2:659770/149‑1 (MQ=255)
                                                                                                       gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  4:326326/149‑1 (MQ=255)
                                                                                                       gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  4:561607/149‑1 (MQ=255)
                                                                                                         ggAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGt                                             <  1:258157/149‑1 (MQ=255)
                                                                                                            aTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCt                                          >  3:87276/1‑149 (MQ=255)
                                                                                                             ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATc                                                     <  3:355825/137‑1 (MQ=255)
                                                                                                             ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATc                                                     >  4:355825/1‑137 (MQ=255)
                                                                                                               gCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          >  3:147122/1‑130 (MQ=255)
                                                                                                               gCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          <  4:147122/130‑1 (MQ=255)
                                                                                                                   cttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGCGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTaa                                   <  3:352393/149‑1 (MQ=255)
                                                                                                                    tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  >  1:316835/1‑149 (MQ=255)
                                                                                                                    tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  <  4:59479/149‑1 (MQ=255)
                                                                                                                      tGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGt                                <  3:552336/149‑1 (MQ=255)
                                                                                                                      tGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGt                                <  1:777222/149‑1 (MQ=255)
                                                                                                                       gATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCAACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTc                               <  4:197365/149‑1 (MQ=255)
                                                                                                                         tGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCaa                             <  2:292964/149‑1 (MQ=255)
                                                                                                                          ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     <  2:226494/140‑1 (MQ=255)
                                                                                                                          ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     >  1:226494/1‑140 (MQ=255)
                                                                                                                           gTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       <  3:707178/89‑1 (MQ=255)
                                                                                                                           gTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       >  4:707178/1‑89 (MQ=255)
                                                                                                                              aTCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactact                        >  1:540581/1‑149 (MQ=255)
                                                                                                                                cTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactactac                      <  2:342959/149‑1 (MQ=255)
                                                                                                                                  tgatgaTGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTg                    >  1:410494/1‑149 (MQ=255)
                                                                                                                                   gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  <  2:320230/134‑1 (MQ=255)
                                                                                                                                   gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  >  1:320230/1‑134 (MQ=255)
                                                                                                                                    atgatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTgag                  <  3:448598/149‑1 (MQ=255)
                                                                                                                                     tgatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTgaga                 >  4:75714/1‑149 (MQ=255)
                                                                                                                                           tcgcTACGCAGGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            >  2:340806/4‑116 (MQ=255)
                                                                                                                                           tcgcTACGCAGGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaa           >  1:746733/4‑149 (MQ=255)
                                                                                                                                           cGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaa           <  2:778482/149‑1 (MQ=255)
                                                                                                                                            gCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGAATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaaa          <  1:434983/149‑1 (MQ=255)
                                                                                                                                             ccTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            <  2:418014/114‑1 (MQ=255)
                                                                                                                                             ccTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            >  1:418014/1‑114 (MQ=255)
                                                                                                                                              cTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAAt        >  2:27464/1‑149 (MQ=255)
                                                                                                                                                 cGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAg     <  2:540581/149‑1 (MQ=255)
                                                                                                                                                  gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAgg    <  4:438662/149‑1 (MQ=255)
                                                                                                                                                  gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAgg    >  2:379075/1‑149 (MQ=255)
                                                                                                                                                    cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTc  <  2:410494/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447902‑3448198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: