Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,051 T→G 6.1% A33A (GCA→GCC rplN ← 50S ribosomal subunit protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,0510TG 6.1% 409.3 / 14.7 115A33A (GCA→GCCrplN50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base T (50/58);  new base G (4/3);  total (54/61)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 116 total aligned reads displayed.

CATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447903‑3448198
                                                                                                                                                    |                                                                                                                                                   
cATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCt                                                                                                                                                     >  3:201823/1‑149 (MQ=255)
       aTCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGc                                                                                                                                              >  1:342959/1‑149 (MQ=255)
        tCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCg                                                                                                                                             >  1:292964/1‑149 (MQ=255)
          gAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGAc                                                                                                                                           >  2:227101/1‑149 (MQ=255)
          gAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACACGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcgac                                                                                                                                          <  4:736948/149‑6 (MQ=255)
           aaGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACg                                                                                                                                          >  2:434983/1‑149 (MQ=255)
            aGCGNATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         >  1:266063/1‑149 (MQ=255)
            aGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACgg                                                                                                                                         <  1:245164/149‑1 (MQ=255)
               gAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGc                                                                                                                                      >  3:750827/1‑149 (MQ=255)
                 aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:456341/149‑1 (MQ=255)
                 aTGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:438879/149‑1 (MQ=255)
                      aGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAc                                                                                                                               >  4:552336/1‑149 (MQ=255)
                        aaCCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCAccc                                                                                                                             >  1:354067/1‑149 (MQ=255)
                                 ggCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTg                                                                                                                    >  1:556081/1‑149 (MQ=255)
                                  gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTTATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  1:770262/149‑1 (MQ=255)
                                  gCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGa                                                                                                                   <  4:796298/149‑1 (MQ=255)
                                    gacgaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATa                                                                                                                 <  1:665301/149‑1 (MQ=255)
                                       gaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  1:567435/127‑1 (MQ=255)
                                       gaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  2:567435/1‑127 (MQ=255)
                                       gaACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCTACGCAGGCGTAggcga                                                                                                                                           >  1:502424/1‑115 (MQ=255)
                                         acacCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACAt                                                                                                            >  2:777222/1‑149 (MQ=255)
                                             ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  2:719836/1‑149 (MQ=255)
                                             ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  2:817261/1‑149 (MQ=255)
                                             ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcg                                                                                                        >  1:659770/1‑149 (MQ=255)
                                             ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                             <  2:12481/112‑3 (MQ=255)
                                             ccTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCAGGCGTAggc                                                                                                                                             >  1:12481/1‑110 (MQ=255)
                                              cttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgc                                                                                                       >  2:740133/1‑149 (MQ=255)
                                               ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    <  2:17478/119‑1 (MQ=255)
                                               ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGa                                                                                                                                    >  1:17478/1‑119 (MQ=255)
                                               ttcttGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAcgcg                                                                                                      >  3:471083/1‑149 (MQ=255)
                                                   tGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGt                                                                                                  <  2:403078/149‑1 (MQ=255)
                                                          caccacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCAACCCAGAACCTTGATACACATTACGCGACGTGCACCgg                                                                                           >  3:197365/1‑149 (MQ=255)
                                                           accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  1:719836/149‑1 (MQ=255)
                                                           accacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGa                                                                                          <  3:590782/149‑1 (MQ=255)
                                                             cacTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGt                                                                                        >  3:9951/1‑149 (MQ=255)
                                                              acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       <  3:124569/149‑1 (MQ=255)
                                                              acTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       >  3:438662/1‑149 (MQ=255)
                                                                        tCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACg                                                                             <  2:556081/149‑1 (MQ=255)
                                                                            cacaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         >  2:258157/1‑149 (MQ=255)
                                                                            cacaTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCa                                                                         <  4:750827/149‑1 (MQ=255)
                                                                               aTCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCa                                                                      >  4:325122/1‑149 (MQ=255)
                                                                                 cACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     >  4:474342/1‑100 (MQ=255)
                                                                                 cACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCtt                                                                                                                     <  3:474342/100‑1 (MQ=255)
                                                                                  aCCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGCGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAg                                                                   >  4:352393/1‑149 (MQ=255)
                                                                                   ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        <  2:456924/95‑1 (MQ=255)
                                                                                   ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                                                                                                        >  1:456924/1‑95 (MQ=255)
                                                                                   ccTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGt                                                                  <  2:892/149‑1 (MQ=255)
                                                                                    ctcttttGACCTTACCACTCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATCCACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTc                                                                 >  4:60573/4‑149 (MQ=255)
                                                                                    cTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTc                                                                          <  1:63081/140‑1 (MQ=255)
                                                                                    cTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTc                                                                          >  2:63081/1‑140 (MQ=255)
                                                                                     ttttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCt                                                                >  4:700401/1‑149 (MQ=255)
                                                                                     ttttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCt                                                                >  1:528171/1‑149 (MQ=255)
                                                                                      tttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTg                                                               <  1:227101/149‑1 (MQ=255)
                                                                                      tttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTg                                                               <  3:64794/149‑1 (MQ=255)
                                                                                       ttttGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGt                                                              <  3:384961/149‑1 (MQ=255)
                                                                                            aCCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATa                                                                    <  2:277804/138‑1 (MQ=255)
                                                                                              cTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  1:683907/1‑149 (MQ=255)
                                                                                              cTTACCACGCGGAACTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGa                                                       >  3:59479/1‑149 (MQ=255)
                                                                                                   cACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGAGACGGTGCGAGCCAACCAGAACCTTGATACACATTACGCGACGTGCTCCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCAtt                                                  <  2:564399/149‑1 (MQ=255)
                                                                                                    aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTa                                                                                                          <  1:320783/92‑1 (MQ=255)
                                                                                                    aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTa                                                                                                          >  2:320783/1‑92 (MQ=255)
                                                                                                    aCGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCAttt                                                 <  3:150179/149‑1 (MQ=255)
                                                                                                      gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  3:105549/149‑1 (MQ=255)
                                                                                                      gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  1:817261/149‑1 (MQ=255)
                                                                                                      gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  4:326326/149‑1 (MQ=255)
                                                                                                      gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  4:561607/149‑1 (MQ=255)
                                                                                                      gcgGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTa                                               <  2:659770/149‑1 (MQ=255)
                                                                                                        ggAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGt                                             <  1:258157/149‑1 (MQ=255)
                                                                                                           aTTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCt                                          >  3:87276/1‑149 (MQ=255)
                                                                                                            ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATc                                                     <  3:355825/137‑1 (MQ=255)
                                                                                                            ttGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATc                                                     >  4:355825/1‑137 (MQ=255)
                                                                                                              gCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCtt                                                          >  3:147122/1‑130 (MQ=255)
                                                                                                               cttcttTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGc                                      <  4:471083/149‑1 (MQ=255)
                                                                                                                   tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  <  4:59479/149‑1 (MQ=255)
                                                                                                                   tttGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  >  1:316835/1‑149 (MQ=255)
                                                                                                                     tGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGt                                <  1:777222/149‑1 (MQ=255)
                                                                                                                      gATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCAACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTc                               <  4:197365/149‑1 (MQ=255)
                                                                                                                        tGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCaa                             <  2:292964/149‑1 (MQ=255)
                                                                                                                         ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     <  2:226494/140‑1 (MQ=255)
                                                                                                                         ggTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCt                                     >  1:226494/1‑140 (MQ=255)
                                                                                                                          gTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       <  3:707178/89‑1 (MQ=255)
                                                                                                                          gTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGtt                                                                                       >  4:707178/1‑89 (MQ=255)
                                                                                                                             aTCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactact                        >  1:540581/1‑149 (MQ=255)
                                                                                                                              tCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAactacta                       <  2:528171/149‑1 (MQ=255)
                                                                                                                                 tgatgaTGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTg                    >  1:410494/1‑149 (MQ=255)
                                                                                                                                  gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  >  1:320230/1‑134 (MQ=255)
                                                                                                                                  gatgatGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAAt                                  <  2:320230/134‑1 (MQ=255)
                                                                                                                                   atgatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTgag                  <  3:448598/149‑1 (MQ=255)
                                                                                                                                    tgatgTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTgaga                 >  4:75714/1‑149 (MQ=255)
                                                                                                                                          tcgcTACGCAGGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            >  2:340806/4‑116 (MQ=255)
                                                                                                                                          tcgcTACGCAGGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaa           >  1:746733/4‑149 (MQ=255)
                                                                                                                                          cGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaa           <  2:778482/149‑1 (MQ=255)
                                                                                                                                           gCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGAATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGaaa          <  1:434983/149‑1 (MQ=255)
                                                                                                                                            ccTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            >  1:418014/1‑114 (MQ=255)
                                                                                                                                            ccTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTg                                            <  2:418014/114‑1 (MQ=255)
                                                                                                                                             cTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAAt        >  2:27464/1‑149 (MQ=255)
                                                                                                                                                cGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAg     <  2:540581/149‑1 (MQ=255)
                                                                                                                                                 gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAgg    <  4:438662/149‑1 (MQ=255)
                                                                                                                                                 gCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAgg    >  2:379075/1‑149 (MQ=255)
                                                                                                                                                   cTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTc  <  2:410494/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTCGGCGACGTTCAGCATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTC  >  NC_000913/3447903‑3448198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: