Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,824 G→C 7.9% P88R (CCC→CGC) ‡ ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,8240GC 7.9% 302.2 / 12.9 87P88R (CCC→CGC) ‡ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (44/36);  new base C (3/4);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGA  >  NC_000913/3601676‑3601969
                                                                                                                                                    |                                                                                                                                                 
cACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTgg                                                                                                                                                   <  4:721148/149‑1 (MQ=255)
     cTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGc                                                                                                                                              <  4:423081/149‑1 (MQ=255)
          tCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCag                                                                                                                                         >  2:814693/1‑149 (MQ=255)
          tCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctg                                                                                                                                         <  4:439397/149‑3 (MQ=255)
           cGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTAaga                                                                                                                                        <  2:417109/149‑1 (MQ=255)
             gCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAgaga                                                                                                                                      >  3:409466/1‑149 (MQ=255)
                 gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATg                                                                                                                                  <  4:799813/149‑1 (MQ=255)
                 gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                          <  1:400774/141‑2 (MQ=255)
                 gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                          >  2:400774/1‑140 (MQ=255)
                    aCTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCg                                                                                                                               <  4:198027/149‑1 (MQ=255)
                       gtgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATa                                                                                                                            >  1:531975/1‑149 (MQ=255)
                            accacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCa                                                                                                                                          >  1:283025/1‑130 (MQ=255)
                            accacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCa                                                                                                                                          <  2:283025/130‑1 (MQ=255)
                            accacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCAt                                                                                                                       >  1:35813/1‑149 (MQ=255)
                               acGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCAc                                                                                                                    <  1:814693/149‑1 (MQ=255)
                               acGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtta                                                                                                                                      <  2:553426/131‑6 (MQ=255)
                               acGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtta                                                                                                                                      >  1:553426/1‑126 (MQ=255)
                                   ccGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGtt                                                                                                                >  4:481527/1‑149 (MQ=255)
                                   ccGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGtt                                                                                                                >  4:787265/1‑149 (MQ=255)
                                   ccGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGtt                                                                                                                >  4:295443/1‑149 (MQ=255)
                                        gcagcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa                                                                                                           <  1:814826/149‑1 (MQ=255)
                                        gcagcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa                                                                                                           >  2:305250/1‑149 (MQ=255)
                                         cagcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAAc                                                                                                          <  2:800098/149‑1 (MQ=255)
                                           gcGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAATGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGt                                                                                                        <  4:656320/149‑1 (MQ=255)
                                            cGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTg                                                                                                       <  3:793043/149‑1 (MQ=255)
                                             gTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTgg                                                                                                      <  1:99597/149‑1 (MQ=255)
                                              tCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGc                                                                                                     >  4:648056/1‑149 (MQ=255)
                                               cATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCg                                                                                                    >  2:600527/1‑149 (MQ=255)
                                                 tCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGaa                                                                                                  <  3:490086/149‑1 (MQ=255)
                                                    tCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACgg                                                                                               >  3:212449/1‑149 (MQ=255)
                                                     ccAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGt                                                                                              >  4:276001/1‑149 (MQ=255)
                                                      cAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATaa                                                                                                                           >  4:281178/1‑119 (MQ=255)
                                                      cAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATaa                                                                                                                           <  3:281178/119‑1 (MQ=255)
                                                      cAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGtt                                                                                             >  4:440579/1‑149 (MQ=255)
                                                       aGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGttt                                                                                            >  2:440626/1‑149 (MQ=255)
                                                        gCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGt                                                                                                        >  2:248533/1‑136 (MQ=255)
                                                        gCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGt                                                                                                        <  1:248533/136‑1 (MQ=255)
                                                        gCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTg                                                                                           >  4:418280/1‑149 (MQ=255)
                                                              tttGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTc                                                                                     <  3:440579/149‑1 (MQ=255)
                                                               ttGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCa                                                                                    <  3:631887/149‑1 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa                                                                                                                              >  2:456863/1‑103 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa                                                                                                                              <  1:456863/103‑1 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                >  2:404430/1‑149 (MQ=255)
                                                                   aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                <  1:253024/149‑1 (MQ=255)
                                                                    gATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATcc                                                                               <  4:88486/149‑1 (MQ=255)
                                                                     aTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt                                                                              >  1:589795/1‑149 (MQ=255)
                                                                     aTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt                                                                              >  4:658688/1‑149 (MQ=255)
                                                                           aGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATg                                                                        >  4:433010/1‑149 (MQ=255)
                                                                            gTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCttt                                                                                       >  1:714978/1‑133 (MQ=255)
                                                                            gTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCttt                                                                                       <  2:714978/133‑1 (MQ=255)
                                                                                tttGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCg                                                                   >  3:301633/1‑149 (MQ=255)
                                                                                tttGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCg                                                                   >  4:221333/1‑149 (MQ=255)
                                                                                  tGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTg                                                                 <  4:745068/149‑1 (MQ=255)
                                                                                      gTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg                                                             <  1:305250/149‑1 (MQ=255)
                                                                                       tGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAgg                                                            <  2:35813/149‑1 (MQ=255)
                                                                                         aCGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCa                                                          <  2:589795/149‑1 (MQ=255)
                                                                                           ggATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATa                                                        <  3:477581/149‑1 (MQ=255)
                                                                                           ggATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATa                                                        >  3:300996/1‑149 (MQ=255)
                                                                                                 taCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCAc                                                  <  3:787265/149‑1 (MQ=255)
                                                                                                 taCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCAc                                                  <  3:481527/149‑1 (MQ=255)
                                                                                                          gccgccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                         <  4:301633/149‑1 (MQ=255)
                                                                                                             gccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGaa                                      >  4:536929/1‑149 (MQ=255)
                                                                                                                 ggTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACg                                  >  1:84886/1‑149 (MQ=255)
                                                                                                                  gTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGt                                 >  2:58458/1‑149 (MQ=255)
                                                                                                                   ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGtt                                >  1:305600/1‑149 (MQ=255)
                                                                                                                   ttAACGTTTTTCTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGtt                                >  3:456613/1‑149 (MQ=255)
                                                                                                                    tAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTg                               <  2:632010/149‑1 (MQ=255)
                                                                                                                       cGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGtt                            >  2:545536/1‑149 (MQ=255)
                                                                                                                        gTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGttt                           >  2:755145/1‑149 (MQ=255)
                                                                                                                        gTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGttt                           >  2:511943/1‑149 (MQ=255)
                                                                                                                        gTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGttt                           >  2:454691/1‑149 (MQ=255)
                                                                                                                         tttttGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGtttt                          >  4:255318/1‑149 (MQ=255)
                                                                                                                                     atatAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGt                             <  4:30359/134‑1 (MQ=255)
                                                                                                                                     atatAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGt                             >  3:30359/1‑134 (MQ=255)
                                                                                                                                      tataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGt             >  2:35874/1‑149 (MQ=255)
                                                                                                                                      tataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGt             >  4:145189/1‑149 (MQ=255)
                                                                                                                                       ataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg                                                             >  4:199917/1‑100 (MQ=255)
                                                                                                                                       ataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg                                                             <  3:199917/100‑1 (MQ=255)
                                                                                                                                       ataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtt            >  1:103995/1‑149 (MQ=255)
                                                                                                                                         aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt          <  1:511943/149‑1 (MQ=255)
                                                                                                                                         aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt          >  4:151760/1‑149 (MQ=255)
                                                                                                                                          tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGa                      >  1:788868/4‑136 (MQ=255)
                                                                                                                                          tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGa                      <  2:788868/133‑1 (MQ=255)
                                                                                                                                              aCGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGa     <  2:531975/149‑1 (MQ=255)
                                                                                                                                               cGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAg    <  4:456613/149‑1 (MQ=255)
                                                                                                                                                 cTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGa  <  3:658688/149‑1 (MQ=255)
                                                                                                                                                  tGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGa  >  2:236620/1‑148 (MQ=255)
                                                                                                                                                  tGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGa  <  1:236620/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CACGCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGA  >  NC_000913/3601676‑3601969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: