Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,895,324 A→G 5.5% I429T (ATC→ACC)  adeP ← adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,895,3240AG 5.5% 240.6 / 3.4 73I429T (ATC→ACC) adePadenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base G (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

ACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAAGAT  >  NC_000913/3895180‑3895471
                                                                                                                                                |                                                                                                                                                   
aCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTAc                                                                                                                                                 >  4:40934/1‑149 (MQ=255)
aCCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTAc                                                                                                                                                 <  1:784235/149‑1 (MQ=255)
       ttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACggg                                                                                                                                          <  1:534017/149‑1 (MQ=255)
           tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                                                                                                                      <  4:91531/149‑1 (MQ=255)
           tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                                                                                                                      <  2:757970/149‑1 (MQ=255)
             aGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGa                                                                                                                                    >  3:495544/1‑149 (MQ=255)
               tttATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATc                                                                                                                                  >  2:428989/1‑149 (MQ=255)
                                 aTCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                                                                                                                      <  2:733901/127‑1 (MQ=255)
                                 aTCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                                                                                                                      >  1:733901/1‑127 (MQ=255)
                                 aTCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc                                                                                                                >  4:647049/1‑149 (MQ=255)
                                      aataaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAttt                                                                                                           >  3:346608/1‑149 (MQ=255)
                                        taaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCATGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtc                                                                                                         >  3:176681/1‑149 (MQ=255)
                                        taaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtc                                                                                                         <  4:495544/149‑1 (MQ=255)
                                            aaaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtcatca                                                                                                     >  1:357750/1‑149 (MQ=255)
                                                   ccAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTa                                                                                              <  4:312217/149‑1 (MQ=255)
                                                     aGCTATCATGCATGGAGGGTTTTTAACGCGCTATAGATTTTAGTGAGCGTCGTTAAAGACAGTCTTCAAGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATAACGCAGACGTCCGGTCCCGATTTTCATCACGCCGTAgg                                                                                            >  4:538338/1‑149 (MQ=255)
                                                     aGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAgg                                                                                            <  1:350225/149‑1 (MQ=255)
                                                      gCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGga                                                                                           <  1:380094/149‑1 (MQ=255)
                                                      gCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGga                                                                                           <  3:647049/149‑1 (MQ=255)
                                                        aagcatgcatgGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGgaga                                                                                         <  4:176681/146‑1 (MQ=255)
                                                        aaTCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGgaga                                                                                         <  2:357750/149‑1 (MQ=255)
                                                         aTCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGGTAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGAt                                                                                        <  4:631070/149‑1 (MQ=255)
                                                              gcatgGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGc                                                                                   >  4:292771/1‑149 (MQ=255)
                                                                 tgGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCa                                                                                <  3:292771/149‑1 (MQ=255)
                                                                    cGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCa                                                                                                                                 >  1:501273/1‑97 (MQ=255)
                                                                    cGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCa                                                                                                                                 <  2:501273/97‑1 (MQ=255)
                                                                       gTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGa                                                                          >  4:550466/1‑149 (MQ=255)
                                                                        tttttAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGAt                                                                         <  3:40934/149‑1 (MQ=255)
                                                                         ttttAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATa                                                                        >  2:20198/1‑149 (MQ=255)
                                                                          tttAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATAc                                                                       >  3:144743/1‑149 (MQ=255)
                                                                          tttAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATAc                                                                       <  4:346608/149‑1 (MQ=255)
                                                                               cgcgCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCg                                                                  <  1:26954/149‑1 (MQ=255)
                                                                               cgcgCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCg                                                                  <  1:428989/149‑1 (MQ=255)
                                                                                   cTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGga                                                                                           <  3:331159/120‑1 (MQ=255)
                                                                                   cTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGga                                                                                           >  4:331159/1‑120 (MQ=255)
                                                                                    tATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAAt                                                             >  4:560838/1‑149 (MQ=255)
                                                                                    tATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAAt                                                             >  4:484388/1‑149 (MQ=255)
                                                                                     aTCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATc                                                            <  3:241170/149‑1 (MQ=255)
                                                                                          ttttAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGaa                                                       <  4:311320/149‑1 (MQ=255)
                                                                                          ttttAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGaa                                                       <  3:560838/149‑1 (MQ=255)
                                                                                                        gATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         >  4:483774/1‑149 (MQ=255)
                                                                                                        gATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         >  2:369811/1‑149 (MQ=255)
                                                                                                        gATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         >  2:181604/1‑149 (MQ=255)
                                                                                                        gATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         >  4:135004/1‑149 (MQ=255)
                                                                                                         aTAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatcat                                        >  1:763171/1‑149 (MQ=255)
                                                                                                         aTAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatcat                                        >  1:74943/1‑149 (MQ=255)
                                                                                                          tAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCatcatc                                       >  3:60263/1‑149 (MQ=255)
                                                                                                           aaaGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCAtcatca                                      >  2:693714/1‑149 (MQ=255)
                                                                                                             aGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         <  3:103771/144‑1 (MQ=255)
                                                                                                             aGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGcatca                                         >  4:103771/1‑144 (MQ=255)
                                                                                                             aGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCAcg                                    >  2:579492/1‑149 (MQ=255)
                                                                                                             aGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCAcg                                    >  4:280043/1‑149 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGGTTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCt                                                    <  3:168970/130‑1 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGGTTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCt                                                    >  4:168970/1‑130 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGGTTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCt                                                    >  4:369725/1‑130 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGGTTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCt                                                    <  3:369725/130‑1 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCg                                                                  <  2:5103/116‑1 (MQ=255)
                                                                                                                cAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCg                                                                  >  1:5103/1‑116 (MQ=255)
                                                                                                                     ttCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGtaat                            <  3:280043/149‑1 (MQ=255)
                                                                                                                        aGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATaaa                         <  1:20198/149‑1 (MQ=255)
                                                                                                                          gATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATaaa                                                                                     >  3:448411/1‑87 (MQ=255)
                                                                                                                          gATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATaaa                                                                                     <  4:448411/87‑1 (MQ=255)
                                                                                                                          gATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACg                       >  2:374780/1‑149 (MQ=255)
                                                                                                                          gATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACg                       >  2:374344/1‑149 (MQ=255)
                                                                                                                           aTAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAAc                        >  1:19397/1‑147 (MQ=255)
                                                                                                                           aTAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAAc                        <  2:19397/147‑1 (MQ=255)
                                                                                                                            tAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGcc                     <  3:484388/149‑1 (MQ=255)
                                                                                                                             aaaCAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCg                    <  3:483774/149‑1 (MQ=255)
                                                                                                                             aaaCAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCg                    <  1:369811/149‑1 (MQ=255)
                                                                                                                              aaCAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCgg                   <  2:763171/149‑1 (MQ=255)
                                                                                                                              aaCAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCgg                   <  4:60081/149‑1 (MQ=255)
                                                                                                                                cagcagCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGaa                 <  4:144743/149‑1 (MQ=255)
                                                                                                                                        cAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCa         <  2:395385/149‑1 (MQ=255)
                                                                                                                                            gatgatTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTaa     <  4:60263/149‑1 (MQ=255)
                                                                                                                                               gatTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAAGAt  >  1:470782/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGATAAAGCCCAGCGCGATACCTTCGGTAATCGAGAAGCTGAACGGCATCATCACGGCGGTAATAAACGCCGGAACAGATTCAGTAAGAT  >  NC_000913/3895180‑3895471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: