Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,972,574 T→C 5.5% V18A (GTG→GCG)  rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,5740TC 5.5% 238.9 / 3.3 73V18A (GTG→GCG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base T (38/31);  new base C (2/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

TGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCA  >  NC_000913/3972431‑3972722
                                                                                                                                               |                                                                                                                                                    
tGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCACCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGtt                                                                                                                                                 <  3:299824/149‑1 (MQ=255)
   tGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTtata                                                                                                                                              <  3:778059/149‑1 (MQ=255)
   tGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTtata                                                                                                                                              <  3:205269/149‑1 (MQ=255)
      tGGTCGATCATAGTCAGTTCAAAGCTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATatcc                                                                                                                                           >  2:267867/1‑148 (MQ=255)
         tCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATAtcatca                                                                                                                                        >  1:298053/1‑149 (MQ=255)
                 aGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCaacgaaacg                                                                                                                                >  3:152735/1‑149 (MQ=255)
                   tCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAg                                                                                                                              >  3:792359/1‑149 (MQ=255)
                    cAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGc                                                                                                                             <  4:152735/149‑1 (MQ=255)
                          aaaGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGAcgcg                                                                                                                       >  3:297989/1‑149 (MQ=255)
                             gTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCggtg                                                                                                                    <  2:525600/149‑1 (MQ=255)
                                 tCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAg                                                                                                                >  1:188847/1‑149 (MQ=255)
                                    aTGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTgg                                                                                                             >  4:319945/1‑149 (MQ=255)
                                     tGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATatcatc                                                                                                                                         <  4:225273/120‑1 (MQ=255)
                                     tGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATatcatc                                                                                                                                         >  3:225273/1‑120 (MQ=255)
                                         gACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGAt                                                                                                        >  4:605891/1‑149 (MQ=255)
                                           cAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATaa                                                                                                      <  1:57108/149‑1 (MQ=255)
                                              tGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCt                                                                                                   >  3:725960/1‑149 (MQ=255)
                                               gTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCaa                                                                                                                                       <  2:743845/112‑1 (MQ=255)
                                               gTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCaa                                                                                                                                       >  1:743845/1‑112 (MQ=255)
                                                tCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGa                                                                                                 <  1:519259/149‑1 (MQ=255)
                                                   aTCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCt                                                                                              >  3:349480/1‑149 (MQ=255)
                                                          gTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGc                                                                                                                             >  1:550351/1‑111 (MQ=255)
                                                          gTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGc                                                                                                                             <  2:550351/111‑1 (MQ=255)
                                                          gTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCgg                                                                                       >  3:319870/1‑149 (MQ=255)
                                                          gTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCgg                                                                                       >  3:485174/1‑149 (MQ=255)
                                                              gtcgtcGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTtat                                                                                                                                               >  1:562834/1‑89 (MQ=255)
                                                              gtcgtcGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTtat                                                                                                                                               <  2:562834/89‑1 (MQ=255)
                                                               tcgtcgATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAAcc                                                                                  >  3:320828/1‑149 (MQ=255)
                                                                      tGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTc                                                                           >  3:746166/1‑149 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATaa                                                                                                      >  4:152895/1‑119 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATaa                                                                                                      <  3:152895/119‑1 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCt                                                                        <  1:267867/149‑1 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCt                                                                        >  4:178469/1‑149 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCt                                                                        >  1:54662/1‑149 (MQ=255)
                                                                         cAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGCGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCt                                                                        >  4:452528/1‑149 (MQ=255)
                                                                          aaaGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTg                                                                       >  2:692792/1‑149 (MQ=255)
                                                                          aaaGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTg                                                                       >  3:712387/1‑149 (MQ=255)
                                                                             ggAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGc                                                                         >  2:776630/1‑144 (MQ=255)
                                                                             ggAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGc                                                                         <  1:776630/144‑1 (MQ=255)
                                                                                   tGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTc                                                              >  2:803870/1‑149 (MQ=255)
                                                                                        cTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCa                                                         >  4:142917/1‑149 (MQ=255)
                                                                                          gatgaGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCaaa                                                       <  4:792359/149‑1 (MQ=255)
                                                                                            tgaGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagc                                                     <  2:687763/149‑1 (MQ=255)
                                                                                              agaAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGc                                                                                                    <  2:152435/100‑1 (MQ=255)
                                                                                              agaAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGc                                                                                                    >  1:152435/1‑100 (MQ=255)
                                                                                                aaaaaTTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagcgagc                                                 >  2:156243/1‑149 (MQ=255)
                                                                                                aaaaaTTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagcgagc                                                 <  4:746166/149‑1 (MQ=255)
                                                                                                aaaaaTTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagcgagc                                                 <  4:45520/149‑1 (MQ=255)
                                                                                                       cTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGc                                          <  4:349480/149‑1 (MQ=255)
                                                                                                          aTAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCtt                                       <  1:692792/149‑1 (MQ=255)
                                                                                                            aaCAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTg                                     <  3:338267/149‑1 (MQ=255)
                                                                                                                 gtggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGaaa                                <  4:320828/149‑1 (MQ=255)
                                                                                                                 gtggtgCCGGGTTTATTGGCTCGGCGCTGGCGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGaaa                                <  3:452528/149‑1 (MQ=255)
                                                                                                                 gtggtgCCGGGTTTATTGGCTCGGCGCTGGCGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGaaa                                >  2:431463/1‑149 (MQ=255)
                                                                                                                  tggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAg                               <  2:298053/149‑1 (MQ=255)
                                                                                                                   ggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGt                              <  2:54662/149‑1 (MQ=255)
                                                                                                                    gtgCCGGGTTTATTGGCTCGGCGCTGGCGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                             <  1:431463/149‑1 (MQ=255)
                                                                                                                      gCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGa                                                                                      >  2:809479/1‑90 (MQ=255)
                                                                                                                      gCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGa                                                                                      <  1:809479/90‑1 (MQ=255)
                                                                                                                      gCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGa                           >  3:644321/1‑149 (MQ=255)
                                                                                                                        cGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGata                         >  2:473521/1‑149 (MQ=255)
                                                                                                                           gTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAg                               <  2:527321/140‑1 (MQ=255)
                                                                                                                           gTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAg                               >  1:527321/1‑140 (MQ=255)
                                                                                                                           gTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCt                      <  1:803870/149‑1 (MQ=255)
                                                                                                                              tATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCg                   <  3:178469/149‑1 (MQ=255)
                                                                                                                                ttGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                             >  1:80143/1‑137 (MQ=255)
                                                                                                                                ttGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                             <  2:80143/137‑1 (MQ=255)
                                                                                                                                ttGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGAt                 >  3:495894/1‑149 (MQ=255)
                                                                                                                                  ggCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCg               >  3:606455/1‑149 (MQ=255)
                                                                                                                                   gCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCgg              >  1:420786/1‑149 (MQ=255)
                                                                                                                                     tCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGc                                                                                                    <  4:347819/61‑1 (MQ=255)
                                                                                                                                     tCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGc                                                                                                    >  3:347819/1‑61 (MQ=255)
                                                                                                                                          gcTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAAc       >  4:607131/1‑149 (MQ=255)
                                                                                                                                               tGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTGGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                             <  3:682090/122‑1 (MQ=255)
                                                                                                                                               tGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                             >  4:682090/1‑122 (MQ=255)
                                                                                                                                               tGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCa  >  1:394734/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCA  >  NC_000913/3972431‑3972722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: