Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,014,759 C→T 5.1% S569S (AGC→AGT)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,014,7590CT 5.1% 268.2 / 3.4 78S569S (AGC→AGT) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (39/35);  new base T (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCC  >  NC_000913/4014612‑4014907
                                                                                                                                                   |                                                                                                                                                    
gATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGcc                                                                                                                                                     <  3:445761/149‑1 (MQ=255)
gATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGcc                                                                                                                                                     <  2:728731/149‑1 (MQ=255)
 aTTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCg                                                                                                                                                    >  2:522290/1‑149 (MQ=255)
    gTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGa                                                                                                                                                 >  1:275876/1‑149 (MQ=255)
     tCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGaa                                                                                                                                                >  2:539186/1‑149 (MQ=255)
      cATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGaaa                                                                                                                                               <  2:275876/149‑1 (MQ=255)
      cATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGaaa                                                                                                                                               <  4:142489/149‑1 (MQ=255)
       aTTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAAc                                                                                                                                              >  3:488786/1‑149 (MQ=255)
          ggTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCAt                                                                                                                                           >  3:336861/1‑149 (MQ=255)
           gTGACATTAGCCGCCCGGCACCGATTCCCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATc                                                                                                                                          >  4:138559/1‑149 (MQ=255)
           gTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATc                                                                                                                                          <  1:541389/149‑1 (MQ=255)
             gACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGc                                                                                                                                        <  1:522290/149‑1 (MQ=255)
             gACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGc                                                                                                                                        >  1:614819/1‑149 (MQ=255)
              aCATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGcc                                                                                                                                       >  4:723558/1‑149 (MQ=255)
                aTTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCaa                                                                                                                                     >  2:190880/1‑149 (MQ=255)
                     ccgccCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGa                                                                                                                                <  3:433034/149‑1 (MQ=255)
                       gccCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGAtt                                                                                                                              <  1:190880/149‑1 (MQ=255)
                              aCCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTgcgctgg                                                                                                                       >  1:80320/1‑149 (MQ=255)
                                    tACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGc                                                                                                                 <  4:617103/149‑1 (MQ=255)
                                      ccGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGt                                                                                                               <  2:614819/149‑1 (MQ=255)
                                         tGGAGTGGGCGAAGTATGCGCAATCGCTGACCTACAAACCGATGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATTTCAGCCGTGAAACCATCGCCAATCAGACTGCGCTGGCGCTGCGtgat                                                                                                            <  4:654583/149‑1 (MQ=255)
                                          ggAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGtgatg                                                                                                           <  2:295034/149‑1 (MQ=255)
                                            aGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGaa                                                                                                         <  4:754085/149‑1 (MQ=255)
                                             gTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAg                                                                                                        >  2:365491/1‑149 (MQ=255)
                                              tGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGc                                                                                                                                        <  1:321209/116‑1 (MQ=255)
                                              tGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGc                                                                                                                                        >  2:321209/1‑116 (MQ=255)
                                               gggCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTg                                                                                                      <  2:525422/149‑1 (MQ=255)
                                                 gCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGc                                                                                                    <  2:459602/149‑1 (MQ=255)
                                                    aaGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGa                                                                                                 >  2:246040/1‑149 (MQ=255)
                                                         tGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTgg                                                                                            >  4:320457/1‑149 (MQ=255)
                                                            gcAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGAtt                                                                                                                              >  1:25934/1‑112 (MQ=255)
                                                            gcAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGAtt                                                                                                                              <  2:25934/112‑1 (MQ=255)
                                                                     gACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGaa                                                                                >  4:781954/1‑149 (MQ=255)
                                                                        agACAAACCGGAGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTg                                                                             >  3:457241/2‑149 (MQ=255)
                                                                          aCAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGc                                                                           <  1:539186/149‑1 (MQ=255)
                                                                            aaaCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGcat                                                                         <  1:786783/149‑1 (MQ=255)
                                                                             aaCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGt                                                                                                       <  4:748939/118‑1 (MQ=255)
                                                                             aaCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGt                                                                                                       >  3:748939/1‑118 (MQ=255)
                                                                             aaCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGATGGTCGTTCCCGGGTGAAAATGTCAGCCGTGAGACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGCGGCCGATCTCGAAGCCGCTGGATTTGGcatc                                                                        >  3:494552/1‑149 (MQ=255)
                                                                              aCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGcatca                                                                       >  3:1582/1‑149 (MQ=255)
                                                                              aCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGcatca                                                                       >  3:252994/1‑149 (MQ=255)
                                                                              aCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTACTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGtga                                                                                                             >  2:441150/1‑111 (MQ=255)
                                                                              aCCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTACTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGtga                                                                                                             <  1:441150/111‑1 (MQ=255)
                                                                                    gAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATCGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCgaga                                                                 <  4:507135/149‑1 (MQ=255)
                                                                                        gggATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGa                                                             <  1:477107/149‑1 (MQ=255)
                                                                                         ggATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGAc                                                            <  3:379637/149‑1 (MQ=255)
                                                                                         ggATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGAc                                                            <  2:80320/149‑1 (MQ=255)
                                                                                              cTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAAcc                                                       <  4:1582/149‑1 (MQ=255)
                                                                                                           tGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAATCAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGaa                                          <  4:544389/149‑1 (MQ=255)
                                                                                                           tGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGaa                                          <  4:488786/149‑1 (MQ=255)
                                                                                                           tGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGaa                                          <  3:723558/149‑1 (MQ=255)
                                                                                                            gACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAg                                         <  4:173483/149‑1 (MQ=255)
                                                                                                              ccATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGt                                       >  1:386783/1‑149 (MQ=255)
                                                                                                              ccATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGt                                       >  1:777353/1‑149 (MQ=255)
                                                                                                               cATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGtt                                      <  4:336861/149‑1 (MQ=255)
                                                                                                               cATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGtt                                      >  1:259035/1‑149 (MQ=255)
                                                                                                               cATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGtt                                      >  1:707903/1‑149 (MQ=255)
                                                                                                                 tACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTa                                    >  1:659650/1‑149 (MQ=255)
                                                                                                                     ctgctgGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGc                                >  3:621459/1‑149 (MQ=255)
                                                                                                                      tgctgGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCt                               >  1:777075/1‑149 (MQ=255)
                                                                                                                          ggTCGTTCCCGAGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGcgt                           >  1:425020/1‑149 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGTCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAgg                                        <  1:29422/135‑1 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGTCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAgg                                        <  1:115216/135‑1 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGTCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAgg                                        >  2:29422/1‑135 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGTCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAgg                                        >  2:115216/1‑135 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGcgtc                          >  4:285907/1‑149 (MQ=255)
                                                                                                                           gTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGcgtc                          >  1:389303/1‑149 (MQ=255)
                                                                                                                                  ccGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGa                   >  1:665148/1‑149 (MQ=255)
                                                                                                                                  ccGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGa                   >  3:374807/1‑149 (MQ=255)
                                                                                                                                  ccGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGa                   <  2:786701/149‑1 (MQ=255)
                                                                                                                                     cgTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTg                <  3:781954/149‑1 (MQ=255)
                                                                                                                                      gTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAAGTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTgg               <  3:285907/149‑1 (MQ=255)
                                                                                                                                        gAAGATGTCAGCTGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGa             >  3:58051/1‑149 (MQ=255)
                                                                                                                                           gATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGc          <  2:259035/149‑1 (MQ=255)
                                                                                                                                            aTGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGc          <  3:57130/148‑1 (MQ=255)
                                                                                                                                            aTGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGc          >  4:57130/1‑148 (MQ=255)
                                                                                                                                            aTGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCg         >  4:298176/1‑149 (MQ=255)
                                                                                                                                              gTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTa       >  4:553622/1‑149 (MQ=255)
                                                                                                                                               tCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTAt      >  3:65969/1‑149 (MQ=255)
                                                                                                                                                 aGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTAtct    <  4:289519/149‑1 (MQ=255)
                                                                                                                                                   ccGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTcc  >  4:559593/1‑149 (MQ=255)
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GATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCGTGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGATCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACTGGGATGCGTATCTCC  >  NC_000913/4014612‑4014907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑