Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,022,007 C→T 5.5% G21G (GGC→GGT)  tatA → TatABCE protein translocation system subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,022,0070CT 5.5% 308.0 / 5.7 91G21G (GGC→GGT) tatATatABCE protein translocation system subunit
Reads supporting (aligned to +/- strand):  ref base C (47/39);  new base T (3/2);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

CACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGCAGGCGGAT  >  NC_000913/4021859‑4022154
                                                                                                                                                    |                                                                                                                                                   
cacGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGc                                                                                                                                                     <  4:782468/149‑1 (MQ=255)
     tGATTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCaa                                                                                                                                                >  4:799644/1‑149 (MQ=255)
     tGATTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAAGGCGGCTTTGTTTAATCATCATCTAGCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCaa                                                                                                                                                >  4:463702/1‑149 (MQ=255)
       aTTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCaaaa                                                                                                                                              >  4:732534/1‑149 (MQ=255)
        ttttttCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCaaaaa                                                                                                                                             <  3:176614/149‑1 (MQ=255)
          ttttCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGc                                                                                                                                           >  3:374378/1‑149 (MQ=255)
            ttCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTc                                                                                                                                         <  3:704777/149‑1 (MQ=255)
                tCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGATATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCt                                                                                                                                     >  3:215241/1‑149 (MQ=255)
                    tCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCAt                                                                                                                                 >  1:450108/1‑149 (MQ=255)
                     cAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGTACCAAAAAGCTCGGCTCCATc                                                                                                                                >  4:699777/1‑149 (MQ=255)
                           gggCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGATATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTcc                                                                                                                          <  4:215241/149‑1 (MQ=255)
                                 tgtAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCtt                                                                                                                    <  2:407899/149‑1 (MQ=255)
                                  gtAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTg                                                                                                                   <  1:57311/149‑1 (MQ=255)
                                  gtAACGTATAATGCGACTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATGGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTg                                                                                                                   <  2:235803/149‑1 (MQ=255)
                                    aaCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGt                                                                                                                 <  3:799644/149‑1 (MQ=255)
                                    aaCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGt                                                                                                                 >  1:659258/1‑149 (MQ=255)
                                     aCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGTGCCAAAAAGca                                                                                                                                          >  4:405605/1‑122 (MQ=255)
                                     aCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGTGCCAAAAAGca                                                                                                                                          <  3:405605/123‑2 (MQ=255)
                                     aCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTg                                                                                                                >  2:501730/1‑149 (MQ=255)
                                     aCGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTg                                                                                                                <  4:374378/149‑1 (MQ=255)
                                       gTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCg                                                                                                              >  2:134808/1‑149 (MQ=255)
                                        tataATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGt                                                                                                             >  4:741147/1‑149 (MQ=255)
                                         ataATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTc                                                                                                            <  3:250533/149‑1 (MQ=255)
                                         ataATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTc                                                                                                            <  2:484870/149‑1 (MQ=255)
                                            aTGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGa                                                                                                                        >  2:452481/1‑134 (MQ=255)
                                            aTGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGa                                                                                                                        <  1:452481/134‑1 (MQ=255)
                                                   tttGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGc                                                                                                  >  3:547186/1‑149 (MQ=255)
                                                        ttAATCATCATCTACCACAGAGGTACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGTGCCAAAAAGcagta                                                                                                                                       >  4:602200/1‑103 (MQ=255)
                                                        ttAATCATCATCTACCACAGAGGTACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGTGCCAAAAAGcagta                                                                                                                                       <  3:602200/107‑5 (MQ=255)
                                                          aaTCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTaaaa                                                                                           <  4:405791/149‑1 (MQ=255)
                                                            tcatcatcTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTaaaaaa                                                                                         <  1:134808/149‑1 (MQ=255)
                                                             catcatcTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGAt                                                                                                         >  4:94695/1‑132 (MQ=255)
                                                             catcatcTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGAt                                                                                                         <  3:94695/132‑1 (MQ=255)
                                                             catcatcTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAg                                                                                        >  3:421933/1‑149 (MQ=255)
                                                               tcatcTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCa                                                                                      >  1:780228/1‑149 (MQ=255)
                                                                       cacaGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAGAGCAATGAGCga                                                                              >  2:455356/1‑149 (MQ=255)
                                                                        acaGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCgat                                                                             <  1:392601/149‑1 (MQ=255)
                                                                          agagGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCgatga                                                                           >  2:728736/1‑149 (MQ=255)
                                                                            agGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGatgatg                                                                         >  1:187586/1‑149 (MQ=255)
                                                                               aaCATGTATGTGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTCCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAAc                                                                      <  2:187586/149‑1 (MQ=255)
                                                                               aaCATGTATGGGTGGTATCAGTGTTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAAc                                                                      <  1:260254/149‑1 (MQ=255)
                                                                                 cATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGaa                                                                       >  4:435378/1‑146 (MQ=255)
                                                                                 cATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGaa                                                                       <  3:435378/146‑1 (MQ=255)
                                                                                  aTGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCaa                                                                   >  4:62609/1‑149 (MQ=255)
                                                                                      aTGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCa                                                               <  1:501730/149‑1 (MQ=255)
                                                                                         ggtggtATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGa                                                            <  3:366044/149‑1 (MQ=255)
                                                                                          gtggtATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGAt                                                           >  3:395325/1‑149 (MQ=255)
                                                                                          gtggtATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGCTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGAt                                                           >  3:354848/1‑149 (MQ=255)
                                                                                           tggtATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATa                                                          >  3:298082/1‑149 (MQ=255)
                                                                                              tATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATaaaa                                                       <  3:62609/149‑1 (MQ=255)
                                                                                              tATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATaaaa                                                       <  4:547186/149‑1 (MQ=255)
                                                                                                tCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGtt                                                                                                                            >  3:429854/1‑78 (MQ=255)
                                                                                                tCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGtt                                                                                                                            <  4:429854/78‑1 (MQ=255)
                                                                                                 cAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCa                                                    <  1:73978/149‑1 (MQ=255)
                                                                                                  aGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAg                                                   >  1:671019/1‑149 (MQ=255)
                                                                                                  aGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAg                                                   >  2:200466/1‑149 (MQ=255)
                                                                                                    tATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTc                                                 <  4:98454/149‑1 (MQ=255)
                                                                                                          gCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCtt                                                                                                                    >  3:360964/1‑76 (MQ=255)
                                                                                                          gCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCtt                                                                                                                    <  4:360964/76‑1 (MQ=255)
                                                                                                          gCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCa                                                    >  2:505104/1‑140 (MQ=255)
                                                                                                          gCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCAACGGTTCCGATCTTGGTGCGTCGAACAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCa                                                    <  1:505104/140‑1 (MQ=255)
                                                                                                           cAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCATAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGc                                          <  3:463702/149‑1 (MQ=255)
                                                                                                            aGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCt                                         <  2:780228/149‑1 (MQ=255)
                                                                                                              ttATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCgatg                                                                            <  2:371407/112‑1 (MQ=255)
                                                                                                              ttATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCgatg                                                                            >  1:371407/1‑112 (MQ=255)
                                                                                                              ttATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGa                                       >  4:487533/1‑149 (MQ=255)
                                                                                                              ttATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGa                                       >  3:161057/1‑149 (MQ=255)
                                                                                                                 ttGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGAttt                                    >  2:800267/1‑149 (MQ=255)
                                                                                                                    attattGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTAc                                 <  2:72453/149‑1 (MQ=255)
                                                                                                                     ttattGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACt                                >  3:37358/1‑149 (MQ=255)
                                                                                                                     ttattGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACt                                >  3:127236/1‑149 (MQ=255)
                                                                                                                      tattGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTg                               >  4:129434/1‑149 (MQ=255)
                                                                                                                       attGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGc                              <  1:200466/149‑1 (MQ=255)
                                                                                                                       attGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGc                              >  3:639116/1‑149 (MQ=255)
                                                                                                                        ttGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCg                             >  3:228611/1‑149 (MQ=255)
                                                                                                                             gTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAAc                        <  2:671019/149‑1 (MQ=255)
                                                                                                                             gTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAAc                        >  1:17125/1‑149 (MQ=255)
                                                                                                                                aTCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTAt                     >  3:770108/1‑149 (MQ=255)
                                                                                                                                aTCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTAt                     >  2:36128/1‑149 (MQ=255)
                                                                                                                                 tCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATc                    <  2:659258/149‑1 (MQ=255)
                                                                                                                                    ttGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGcc                 <  4:395325/149‑1 (MQ=255)
                                                                                                                                    ttGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGcc                 <  4:421933/149‑1 (MQ=255)
                                                                                                                                         cTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGTTaa            >  2:430473/1‑149 (MQ=255)
                                                                                                                                          tGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAg           >  1:483155/1‑149 (MQ=255)
                                                                                                                                          tGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAg           >  1:39157/1‑149 (MQ=255)
                                                                                                                                          tGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAg           >  3:384735/1‑149 (MQ=255)
                                                                                                                                          tGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAg           >  1:359680/1‑149 (MQ=255)
                                                                                                                                          tGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAg           >  1:136502/1‑149 (MQ=255)
                                                                                                                                           gCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGc          <  2:17125/149‑1 (MQ=255)
                                                                                                                                             tttttGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGCAg        <  4:298082/149‑1 (MQ=255)
                                                                                                                                               tttGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGCAGGc      <  3:487533/149‑1 (MQ=255)
                                                                                                                                                   gCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGCAGGCGGAt  >  1:279645/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAACGTATAATGCGGCTTTGTTTAATCATCATCTACCACAGAGGAACATGTATGGGTGGTATCAGTATTTGGCAGTTATTGATTATTGCCGTCATCGTTGTACTGCTTTTTGGCACCAAAAAGCTCGGCTCCATCGGTTCCGATCTTGGTGCGTCGATCAAAGGCTTTAAAAAAGCAATGAGCGATGATGAACCAAAGCAGGATAAAACCAGTCAGGATGCTGATTTTACTGCGAAAACTATCGCCGATAAGCAGGCGGAT  >  NC_000913/4021859‑4022154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑