Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,066,800 A→G 6.1% F132S (TTT→TCT)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,066,8000AG 6.1% 325.8 / 9.0 99F132S (TTT→TCT) yihPputative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (40/53);  new base G (3/3);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 102 total aligned reads displayed.

ATTCATAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGA  >  NC_000913/4066652‑4066947
                                                                                                                                                    |                                                                                                                                                   
aTTCATAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGaaaa                                                                                                                                                     >  2:267525/1‑149 (MQ=255)
 ttCATAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGaaaaa                                                                                                                                                    >  1:155102/1‑149 (MQ=255)
   cATAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACa                                                                                                                                                  <  4:324546/149‑1 (MQ=255)
    aTAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAg                                                                                                                                                 >  2:436002/1‑149 (MQ=255)
    aTAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAg                                                                                                                                                 >  2:123924/1‑149 (MQ=255)
    aTAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAg                                                                                                                                                 >  1:607727/1‑149 (MQ=255)
     tAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGt                                                                                                                                                <  2:469811/149‑1 (MQ=255)
      aaCTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTc                                                                                                                                               <  4:724201/149‑1 (MQ=255)
      aaCTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTc                                                                                                                                               <  1:292972/149‑1 (MQ=255)
           gCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTaa                                                                                                                                          >  4:790405/1‑149 (MQ=255)
            cACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTaaa                                                                                                                                         <  1:436002/149‑1 (MQ=255)
             aCGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAg                                                                                                                                        <  1:811969/149‑1 (MQ=255)
             aCGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAg                                                                                                                                        >  4:584405/1‑149 (MQ=255)
               gAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCa                                                                                                                                      >  3:590250/1‑149 (MQ=255)
                  tCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATaa                                                                                                                                   <  2:470400/149‑1 (MQ=255)
                    ccACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAAc                                                                                                                                 <  4:446692/149‑1 (MQ=255)
                    ccACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAAc                                                                                                                                 <  4:106046/149‑1 (MQ=255)
                     cACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACa                                                                                                                                <  3:99495/149‑1 (MQ=255)
                     cACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACa                                                                                                                                >  1:459658/1‑149 (MQ=255)
                        cGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGaa                                                                                                                             <  3:770991/149‑1 (MQ=255)
                             acaGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTg                                                                                                                        <  1:653509/149‑1 (MQ=255)
                             acaGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTg                                                                                                                        >  4:720464/1‑149 (MQ=255)
                             acaGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTg                                                                                                                        <  1:270803/149‑1 (MQ=255)
                                gcagcagGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGcc                                                                                                                     <  4:206853/149‑1 (MQ=255)
                                  agcagGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCAt                                                                                                                   <  4:776239/149‑1 (MQ=255)
                                   gcagGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGGTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATc                                                                                                                  >  2:53364/1‑149 (MQ=255)
                                        cccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGt                                                                                                             <  3:790405/149‑1 (MQ=255)
                                         ccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCggt                                                                                                            >  1:458035/1‑147 (MQ=255)
                                         ccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGtt                                                                                                            >  4:146579/1‑149 (MQ=255)
                                         ccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGtt                                                                                                            >  3:508057/1‑149 (MQ=255)
                                         ccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGtt                                                                                                            <  2:607727/149‑1 (MQ=255)
                                         ccAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGtt                                                                                                            <  1:123924/149‑1 (MQ=255)
                                          cAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGttt                                                                                                           >  2:118891/1‑149 (MQ=255)
                                          cAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGttt                                                                                                           >  1:414938/1‑149 (MQ=255)
                                             cGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTAc                                                                                                        <  3:584405/149‑1 (MQ=255)
                                                 gcgcCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    <  1:437615/149‑1 (MQ=255)
                                                 gcgcCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    >  1:626885/1‑149 (MQ=255)
                                                  cgccgcCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTg                                                                                                   <  3:155835/149‑1 (MQ=255)
                                                     cgccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGGTAAAACGGTGatg                                                                                                <  2:458035/149‑3 (MQ=255)
                                                             cGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACgg                                                                                        <  1:258580/149‑1 (MQ=255)
                                                              gCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgt                                                                                       >  4:767156/1‑149 (MQ=255)
                                                                cATGGCGTCAGGGCGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgtgt                                                                                     <  2:404778/149‑1 (MQ=255)
                                                                 aTGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGtgtg                                                                                    >  4:483724/1‑149 (MQ=255)
                                                                         aGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGGATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGACGACaaa                                                                            <  2:434658/149‑1 (MQ=255)
                                                                         aGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACaaa                                                                            <  4:366010/149‑1 (MQ=255)
                                                                         aGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACaaa                                                                            >  1:804646/1‑149 (MQ=255)
                                                                          gTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAg                                                                           <  1:6274/149‑1 (MQ=255)
                                                                             aGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    >  3:321818/1‑121 (MQ=255)
                                                                             aGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    <  4:321818/121‑1 (MQ=255)
                                                                             aGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    <  2:342446/121‑1 (MQ=255)
                                                                             aGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                    >  1:342446/1‑121 (MQ=255)
                                                                             aGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTg                                                                        <  2:803357/149‑1 (MQ=255)
                                                                               gcgcGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGt                                                                          >  4:343257/1‑145 (MQ=255)
                                                                               gcgcGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGt                                                                          <  3:343257/145‑1 (MQ=255)
                                                                                     tcgttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGc                                                                <  4:617541/149‑1 (MQ=255)
                                                                                         tGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGt                                                            <  3:353411/149‑1 (MQ=255)
                                                                                         tGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGt                                                            <  1:644016/149‑1 (MQ=255)
                                                                                          ggggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTa                                                           >  3:220695/1‑149 (MQ=255)
                                                                                          ggggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTa                                                           >  3:340650/1‑149 (MQ=255)
                                                                                          ggggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTa                                                           >  2:500149/1‑149 (MQ=255)
                                                                                           gggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTaa                                                          <  2:414938/149‑1 (MQ=255)
                                                                                             gTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAgg                                                        >  4:12591/1‑149 (MQ=255)
                                                                                                 ttGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGAc                                                    <  3:12591/149‑1 (MQ=255)
                                                                                                     tGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCggg                                                <  4:590250/149‑1 (MQ=255)
                                                                                                       aTAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGaa                                              >  2:506760/1‑149 (MQ=255)
                                                                                                       aTAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGaa                                              <  3:98064/149‑1 (MQ=255)
                                                                                                        tAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAAt                                             >  1:763235/1‑149 (MQ=255)
                                                                                                         aGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATg                                            >  2:404599/1‑149 (MQ=255)
                                                                                                          gCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGa                                           >  1:446775/1‑149 (MQ=255)
                                                                                                           cGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          <  2:626885/149‑1 (MQ=255)
                                                                                                           cGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          <  4:156853/149‑1 (MQ=255)
                                                                                                             ggAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGc                                        <  1:267525/149‑1 (MQ=255)
                                                                                                                 ccATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATAc                                    >  3:780172/1‑149 (MQ=255)
                                                                                                                  cATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACa                                   <  4:180315/149‑1 (MQ=255)
                                                                                                                     tGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                <  3:720464/149‑1 (MQ=255)
                                                                                                                        gcCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgtgt                                                                                     <  3:341811/93‑1 (MQ=255)
                                                                                                                        gcCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgtgt                                                                                     >  4:341811/1‑93 (MQ=255)
                                                                                                                         ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaa                            <  3:146579/149‑1 (MQ=255)
                                                                                                                          cGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaaa                           <  1:118891/149‑1 (MQ=255)
                                                                                                                              ggAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAg                                                         >  4:634768/1‑115 (MQ=255)
                                                                                                                              ggAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAg                                                         <  3:634768/115‑1 (MQ=255)
                                                                                                                              ggAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGa                        <  3:89029/148‑1 (MQ=255)
                                                                                                                              ggAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGAc                       >  1:47010/1‑149 (MQ=255)
                                                                                                                               gAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          >  3:770520/1‑129 (MQ=255)
                                                                                                                               gAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          <  4:770520/129‑1 (MQ=255)
                                                                                                                               gAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACg                      >  1:359542/1‑149 (MQ=255)
                                                                                                                               gAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACg                      >  2:108223/1‑149 (MQ=255)
                                                                                                                                  cAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGaa                   <  2:155102/149‑1 (MQ=255)
                                                                                                                                    gTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACt                 >  4:602910/1‑149 (MQ=255)
                                                                                                                                        atcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          >  1:484483/1‑120 (MQ=255)
                                                                                                                                        atcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGAc                                          <  2:484483/120‑1 (MQ=255)
                                                                                                                                         tcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCt            <  2:459658/149‑1 (MQ=255)
                                                                                                                                             gCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTg        <  1:53364/149‑1 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACTGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                <  3:495238/123‑1 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                >  2:60050/1‑123 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                >  4:703017/1‑123 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                >  4:495238/1‑123 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                <  3:703017/123‑1 (MQ=255)
                                                                                                                                               tGAAAGACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGaa                                <  1:60050/123‑1 (MQ=255)
                                                                                                                                                   aaaCAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGa  >  4:17157/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTCATAACTGGCACGAATCCCACCGTGCACAGCAGCAGGCCCAGCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGA  >  NC_000913/4066652‑4066947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: