Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,213,131 C→T 10.7% noncoding (92/120 nt) rrfE → 5S ribosomal RNA of rrnE operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,1310CT10.7% 217.2 / 14.7 75noncoding (92/120 nt)rrfE5S ribosomal RNA of rrnE operon
Reads supporting (aligned to +/- strand):  ref base C (39/28);  new base T (6/2);  total (45/30)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

AGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATT  >  NC_000913/4213015‑4213272
                                                                                                                    |                                                                                                                                             
aGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAa                                                                                                               >  2:712108‑M2/1‑148 (MQ=255)
aGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAa                                                                                                               <  4:755725‑M2/149‑2 (MQ=255)
     cGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaa                                                                                                          >  3:755725‑M2/1‑143 (MQ=255)
     cGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaa                                                                                                          >  4:418036‑M2/1‑143 (MQ=255)
         cTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaa                                                                                                      >  2:643183‑M2/1‑139 (MQ=255)
           gATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCGAATTAGAAAAAcc                                                                                                    >  4:682430/1‑149 (MQ=255)
           gATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaag                                                                                                    >  4:784197‑M2/1‑137 (MQ=255)
            aTAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAccc                                                                                                   <  1:537923/149‑1 (MQ=38)
            aTAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAccc                                                                                                   >  2:537923/1‑149 (MQ=38)
             tAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggc                                                                                                  <  4:583741‑M2/149‑15 (MQ=255)
              aaaaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg                                                                                                 >  4:511923/1‑149 (MQ=40)
              aaaaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg                                                                                                 <  3:511923/149‑1 (MQ=40)
                aaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGt                                                                                               <  1:126282/149‑1 (MQ=255)
                 aCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTc                                                                                              <  4:749849/149‑1 (MQ=255)
                     aaTTTGCCTGGCGGCATTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGTCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcgcagt                                                                                             >  2:297655‑M2/1‑127 (MQ=255)
                     aaTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATa                                                                                          >  4:403139/1‑149 (MQ=255)
                      aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa                                                                                         <  3:682430/149‑1 (MQ=255)
                         tGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGc                                                                                      <  3:133424/149‑1 (MQ=255)
                            cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                   >  3:284129/1‑149 (MQ=255)
                            cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                   >  3:470752/1‑149 (MQ=255)
                               gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagc                                                                                >  1:514169‑M2/1‑117 (MQ=255)
                               gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagc                                                                                >  1:483371‑M2/1‑117 (MQ=255)
                                  gcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgagcctt                                                                             >  3:391681‑M2/1‑114 (MQ=255)
                                   cAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagccttt                                                                            >  2:41971‑M2/1‑113 (MQ=255)
                                    aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagcctttc                                                                           >  3:330994‑M2/1‑112 (MQ=255)
                                             gtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGACATCAAataaaacaaaaggcccagtcttccgactgagccttttgttttattt                                                                  >  4:703260‑M2/1‑103 (MQ=255)
                                                gtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaaaaa                                                                                                      <  4:76824/110‑1 (MQ=35)
                                                gtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaaaaa                                                                                                      >  3:76824/1‑110 (MQ=35)
                                                  cccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattatt                                                             >  3:191645/1‑149 (MQ=255)
                                                   ccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgagcct                                                                              <  3:91803‑M2/131‑35 (MQ=255)
                                                   ccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgagcct                                                                              >  4:91803‑M2/1‑97 (MQ=255)
                                                      ccagACCCCAGGCCGAACTCAGAAGTGAAACGACGTAGGTCCGAGGGTCGTGTGGGGTCTCCCCATGCGAGAGTAGGAAACTTCCAGGCATCAAATTAGATAAACCCCCGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa                                                         >  4:711482/4‑149 (MQ=18)
                                                      ccTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa                                                         <  3:172329/149‑1 (MQ=255)
                                                         gACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGt                                                                                               <  4:338592/108‑1 (MQ=255)
                                                         gACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGt                                                                                               >  3:338592/1‑108 (MQ=255)
                                                             ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttt                                                                           >  1:713801/1‑124 (MQ=255)
                                                             ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGaa                                                  >  2:106297/1‑149 (MQ=255)
                                                             ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGaa                                                  >  3:576705/1‑149 (MQ=255)
                                                             ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGGAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGtttttt                                                                           <  2:713801/124‑1 (MQ=255)
                                                                      aCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAtctc                                         <  4:576705/149‑1 (MQ=255)
                                                                       cTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAat                                                                                                              <  2:590471‑M2/79‑3 (MQ=255)
                                                                       cTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAat                                                                                                              >  1:590471‑M2/1‑77 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagcctttc                                                                           <  2:236212‑M2/113‑38 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagcctttc                                                                           >  1:236212‑M2/1‑76 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa                                                                                         >  4:716763/1‑99 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa                                                                                         <  3:716763/99‑1 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                 <  3:729644/139‑1 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                 >  4:729644/1‑139 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                       >  3:602567/1‑149 (MQ=255)
                                                                        tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                       <  2:657737/149‑1 (MQ=255)
                                                                          aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGt                                     >  1:793745/1‑149 (MQ=255)
                                                                            aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagcctttcgt                                                                         >  3:70420‑M2/1‑72 (MQ=255)
                                                                            aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtcttccgactgagcctttcgt                                                                         <  4:70420‑M2/111‑40 (MQ=255)
                                                                            aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCa                                   <  3:403139/149‑1 (MQ=255)
                                                                              gTGAAACGCCGTAGCGCCGCTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa                                                                                         <  3:677550/93‑1 (MQ=255)
                                                                              gTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCTCGGTCCATaa                                                                                         >  4:677550/1‑93 (MQ=255)
                                                                                 aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGc                                                                                      <  4:101404/93‑1 (MQ=255)
                                                                                 aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGc                                                                                      >  3:101404/1‑93 (MQ=255)
                                                                                 aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                              >  3:799374/1‑149 (MQ=255)
                                                                                 aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                              >  1:130104/1‑149 (MQ=255)
                                                                                 aaaCGCCGTAGCGCCGACGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                              >  2:17163/1‑149 (MQ=255)
                                                                                  aaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTg                             >  2:374744/1‑149 (MQ=255)
                                                                                   aCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGc                                                                                      <  2:804019/91‑1 (MQ=255)
                                                                                   aCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGc                                                                                      >  1:804019/1‑91 (MQ=255)
                                                                                   aCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGt                            <  1:374744/149‑1 (MQ=255)
                                                                                    cGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGta                           <  1:106297/149‑1 (MQ=255)
                                                                                                 aTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                   <  1:615476/80‑1 (MQ=255)
                                                                                                 aTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCgg                                                                                   >  2:615476/1‑80 (MQ=255)
                                                                                                   ggTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgag                                                                                 >  2:124015‑M2/1‑49 (MQ=255)
                                                                                                   ggTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAataaaacgaaaggcccagtctttcgactgag                                                                                 <  1:124015‑M2/80‑32 (MQ=255)
                                                                                                     tAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTa          >  3:164232/1‑149 (MQ=255)
                                                                                                        tgtgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATc       <  4:470752/149‑1 (MQ=255)
                                                                                                             ggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAtt  >  4:38515/1‑149 (MQ=255)
                                                                                                                 tcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaa     <  2:285797/142‑1 (MQ=255)
                                                                                                                 tcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaa     >  1:285797/1‑142 (MQ=255)
                                                                                                                   cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAgg                                                >  1:702240/1‑97 (MQ=255)
                                                                                                                   cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAgg                                                <  2:702240/97‑1 (MQ=255)
                                                                                                                    |                                                                                                                                             
AGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATT  >  NC_000913/4213015‑4213272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: