Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,581 G→C 5.4% T398S (ACT→AGT)  dcuA ← C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,5810GC 5.4% 331.5 / 7.5 93T398S (ACT→AGT) dcuAC4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base G (50/38);  new base C (3/2);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCG  >  NC_000913/4365433‑4365724
                                                                                                                                                    |                                                                                                                                               
gggAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtag                                                                                                                                                 >  4:237418/1‑149 (MQ=255)
 ggAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagt                                                                                                                                                <  3:764709/149‑1 (MQ=255)
    aagaacgCGGGGCGTGAACGCCTCGCGATTGATCATTACAGCATGAAGCTACGCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagtaac                                                                                                                                             <  4:756465/149‑3 (MQ=255)
        tcgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgtg                                                                                                                                         >  4:55733/3‑149 (MQ=255)
        aagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgtg                                                                                                                                         >  2:372822/1‑149 (MQ=255)
         cgCGGGGCGTTCACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGATGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCCATACGGGTAGTACCCgtgt                                                                                                                                        >  1:575581/2‑149 (MQ=255)
         agCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgtgt                                                                                                                                        <  3:237418/149‑1 (MQ=255)
            ggggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcat                                                                                                                                     >  3:371658/1‑149 (MQ=255)
              ggCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc                                                                                                                                   <  2:525976/149‑1 (MQ=255)
                  tGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCt                                                                                                                               <  2:532361/149‑1 (MQ=255)
                  tGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCt                                                                                                                               >  4:480911/1‑149 (MQ=255)
                     aCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTa                                                                                                                            <  1:675241/149‑1 (MQ=255)
                       gCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTAcc                                                                                                                          >  4:304011/1‑149 (MQ=255)
                         cccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgc                                                                                                                        >  1:725985/1‑149 (MQ=255)
                         cccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgc                                                                                                                        >  2:360915/1‑149 (MQ=255)
                          ccGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgca                                                                                                                       >  2:292088/1‑149 (MQ=255)
                            gcgATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgcagc                                                                                                                     <  2:725985/149‑1 (MQ=255)
                                ttGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAAcc                                                                                                                 >  2:490605/1‑149 (MQ=255)
                                   aTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGc                                                                                                              <  3:161937/149‑1 (MQ=255)
                                   aTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGc                                                                                                              >  2:207883/1‑149 (MQ=255)
                                    tCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCg                                                                                                             >  2:481432/1‑149 (MQ=255)
                                    tCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGCGCAACACCCAGACTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCg                                                                                                             >  4:589339/1‑149 (MQ=255)
                                       ttACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCg                                                                                                          <  3:589339/149‑1 (MQ=255)
                                        tACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCgg                                                                                                         <  3:573482/149‑1 (MQ=255)
                                         aCAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCggg                                                                                                        <  2:575581/149‑1 (MQ=255)
                                              aTGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGt                                                                                                   >  1:78066/1‑149 (MQ=255)
                                                 aaGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCgg                                                                                                >  3:768570/1‑149 (MQ=255)
                                                  aGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGc                                                                                               >  3:391347/1‑149 (MQ=255)
                                                   gCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCa                                                                                              >  1:48346/1‑149 (MQ=255)
                                                      aCCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACTGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  2:252644/149‑1 (MQ=255)
                                                      aCCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  2:45101/149‑1 (MQ=255)
                                                      aCCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  3:480911/149‑1 (MQ=255)
                                                       cccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  4:725739/148‑1 (MQ=255)
                                                       cccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           >  3:725739/1‑148 (MQ=255)
                                                       cccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt                                                                                          <  1:207883/149‑1 (MQ=255)
                                                        ccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATg                                                                                         >  3:557822/1‑149 (MQ=255)
                                                          aGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           >  3:801893/1‑145 (MQ=255)
                                                          aGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  4:801893/145‑1 (MQ=255)
                                                           gCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAc                                                                                      <  4:391347/149‑1 (MQ=255)
                                                               gaagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagac                                                                                  >  4:590552/1‑149 (MQ=255)
                                                                aagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGAAGAATGAAcagacc                                                                                 <  3:55733/149‑1 (MQ=255)
                                                                  gccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccag                                                                               >  4:276638/1‑149 (MQ=255)
                                                                   ccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccaga                                                                              <  4:371658/149‑1 (MQ=255)
                                                                   ccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccaga                                                                              >  3:15335/1‑149 (MQ=255)
                                                                     gaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGaca                                                                            >  3:146953/1‑149 (MQ=255)
                                                                      aagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGacac                                                                           <  1:372822/149‑1 (MQ=255)
                                                                           aaCGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAg                                                                      >  1:559634/1‑149 (MQ=255)
                                                                            aCGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGc                                                                     <  3:488521/149‑1 (MQ=255)
                                                                               gCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACATAACAGACACCGCAGCGaa                                                                  >  1:628409/1‑149 (MQ=255)
                                                                                  agagCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAaaga                                                               >  4:737245/4‑149 (MQ=255)
                                                                                   gggCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTc                                                                                                           >  1:156015/1‑104 (MQ=255)
                                                                                   gggCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTc                                                                                                           <  2:156015/104‑1 (MQ=255)
                                                                                       aaCACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCaa                                                          <  2:78066/149‑1 (MQ=255)
                                                                                         cacCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAca                                                        <  1:481432/149‑1 (MQ=255)
                                                                                         cacCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAca                                                        <  3:737245/149‑1 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           >  1:271272/1‑111 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa                                                                                           <  2:271272/111‑1 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                                     >  1:602775/1‑149 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                                     >  2:174934/1‑149 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                                     >  2:242909/1‑149 (MQ=255)
                                                                                            ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAACAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcg                                                     <  1:292088/149‑1 (MQ=255)
                                                                                             cAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcgg                                                    >  2:176044/1‑149 (MQ=255)
                                                                                                   aCCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAAcagcggtcagcg                                              <  4:599729/149‑1 (MQ=255)
                                                                                                             aagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGtt                                    <  3:276638/149‑1 (MQ=255)
                                                                                                              agaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt                                                                                          <  1:366100/94‑1 (MQ=255)
                                                                                                              agaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt                                                                                          >  2:366100/1‑94 (MQ=255)
                                                                                                               gaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGaaa                                        >  1:37743/1‑143 (MQ=255)
                                                                                                               gaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGaaa                                        <  2:37743/143‑1 (MQ=255)
                                                                                                               gaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa                                  >  3:249418/1‑149 (MQ=255)
                                                                                                               gaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa                                  >  2:417697/1‑149 (MQ=255)
                                                                                                                 aCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGt                                <  1:417697/149‑1 (MQ=255)
                                                                                                                       ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCaga                          <  3:23967/149‑1 (MQ=255)
                                                                                                                       ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCaga                          <  4:249418/149‑1 (MQ=255)
                                                                                                                        gTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAaagaa                                                              <  3:458572/112‑1 (MQ=255)
                                                                                                                        gTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAaagaa                                                              >  4:458572/1‑112 (MQ=255)
                                                                                                                         ttGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGaaa                                                                 <  2:46974/108‑1 (MQ=255)
                                                                                                                         ttGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGaaa                                                                 >  1:46974/1‑108 (MQ=255)
                                                                                                                         ttGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGc                        >  2:616893/1‑149 (MQ=255)
                                                                                                                         ttGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGc                        >  4:352021/1‑149 (MQ=255)
                                                                                                                              gACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCg                   <  2:48346/149‑1 (MQ=255)
                                                                                                                              gACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCg                   >  3:285981/1‑149 (MQ=255)
                                                                                                                              gACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCg                   >  3:404021/1‑149 (MQ=255)
                                                                                                                                 gAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCa                >  1:260641/1‑149 (MQ=255)
                                                                                                                                     ttACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAg            >  2:654418/1‑149 (MQ=255)
                                                                                                                                      tACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGt           <  4:146953/149‑1 (MQ=255)
                                                                                                                                       aCCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTg          >  1:471160/1‑149 (MQ=255)
                                                                                                                                         tgacaCGGGTACTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCt        >  3:284091/5‑149 (MQ=255)
                                                                                                                                          gacaCGGGTACTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAg                                                                      >  4:396499/4‑86 (MQ=255)
                                                                                                                                          gacaCGGGTACTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAg                                                                      <  3:396499/83‑1 (MQ=255)
                                                                                                                                          gATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCtt       >  3:404312/1‑149 (MQ=255)
                                                                                                                                           aTACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCttt      >  2:599838/1‑149 (MQ=255)
                                                                                                                                             aCGGGTACTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAg                                                                                             >  1:201394/1‑60 (MQ=255)
                                                                                                                                             aCGGGTACTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAg                                                                                             <  2:201394/60‑1 (MQ=255)
                                                                                                                                              cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGc   <  2:602775/149‑1 (MQ=255)
                                                                                                                                               gggTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCg  >  4:348307/1‑149 (MQ=255)
                                                                                                                                               gggTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCg  >  2:740337/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCG  >  NC_000913/4365433‑4365724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: