Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,448,274 G→A 5.6% intergenic (+39/‑173) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,2740GA 5.6% 236.8 / 3.3 71intergenic (+39/‑173)ytfP/chpSGGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base A (2/2);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATAC  >  NC_000913/4448127‑4448422
                                                                                                                                                   |                                                                                                                                                    
ccAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGc                                                                                                                                                     <  2:726918/149‑1 (MQ=255)
       aTTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAgacga                                                                                                                                              >  4:581090/1‑149 (MQ=255)
                 cGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCt                                                                                                                                    <  2:753082/149‑1 (MQ=255)
                  gTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTg                                                                                                                                   >  4:66344/1‑149 (MQ=255)
                   tACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGt                                                                                                                                  <  1:253659/149‑1 (MQ=255)
                     cGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGttt                                                                                                                                <  4:198349/149‑1 (MQ=255)
                            gCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAAtt                                                                                                                         >  1:673200/1‑149 (MQ=255)
                            gCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAAtt                                                                                                                         >  3:444811/1‑149 (MQ=255)
                             cATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTACGAGACGACTCGCATTCTGTTTTGTAATTc                                                                                                                        >  2:130178/1‑149 (MQ=255)
                             cATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTACGAGACGACTCGCATTCTGTTTTGTAATTc                                                                                                                        >  2:2424/1‑149 (MQ=255)
                              aTGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTcc                                                                                                                       >  3:729942/1‑149 (MQ=255)
                                ggATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCt                                                                                                                     >  4:107520/1‑149 (MQ=255)
                                    gTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTc                                                                                                                    >  3:464456/1‑146 (MQ=255)
                                    gTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTc                                                                                                                    <  4:464456/146‑1 (MQ=255)
                                     tACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTaa                                                                                                                           <  2:772206/138‑1 (MQ=255)
                                     tACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTaa                                                                                                                           >  1:772206/1‑138 (MQ=255)
                                      aCGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCtt                                                                                                               >  4:131686/1‑149 (MQ=255)
                                        gTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTcc                                                                                                                       <  3:605957/139‑1 (MQ=255)
                                        gTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTcc                                                                                                                       >  4:605957/1‑139 (MQ=255)
                                        gTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTACGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCtttt                                                                                                             <  1:130178/149‑1 (MQ=255)
                                        gTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTACGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCtttt                                                                                                             <  1:2424/149‑1 (MQ=255)
                                           tATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCt                                                                                                          <  4:291845/149‑1 (MQ=255)
                                           tATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCt                                                                                                          <  4:669182/149‑1 (MQ=255)
                                              cAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCtttt                                                                                                       <  4:706349/149‑1 (MQ=255)
                                              cAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCtttt                                                                                                       <  2:458695/149‑1 (MQ=255)
                                              cAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCtttt                                                                                                       <  4:612953/149‑1 (MQ=255)
                                                aCGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCt                                                                                                                                    <  2:648118/118‑1 (MQ=255)
                                                aCGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCt                                                                                                                                    >  1:648118/1‑118 (MQ=255)
                                                    cccGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTcc                                                                                                 <  2:279932/149‑1 (MQ=255)
                                                     ccGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCg                                                                                                >  2:556743/1‑149 (MQ=255)
                                                           aTGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGc                                                                                          <  1:725678/149‑1 (MQ=255)
                                                           aTGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGc                                                                                          <  3:109909/149‑1 (MQ=255)
                                                              gATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGtttt                                                                                                                               <  2:244377/109‑1 (MQ=255)
                                                              gATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGtttt                                                                                                                               >  1:244377/1‑109 (MQ=255)
                                                               aTTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTc                                                                                      >  2:288050/1‑149 (MQ=255)
                                                               aTTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTc                                                                                      <  3:581090/149‑1 (MQ=255)
                                                               aTTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTc                                                                                      >  3:518257/1‑149 (MQ=255)
                                                                  aaaGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCat                                                                                   <  4:729942/149‑1 (MQ=255)
                                                                   aaGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCata                                                                                  <  3:66344/149‑1 (MQ=255)
                                                                       tAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTa                                                                              >  1:447304/1‑149 (MQ=255)
                                                                       tAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTa                                                                              >  3:652540/1‑149 (MQ=255)
                                                                              aaGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTCTCTCTCCGAGCCGCTTTCCATATCTATTAACGc                                                                       >  2:430231/1‑149 (MQ=255)
                                                                                      aCTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATaaaaaa                                                               <  3:107520/149‑1 (MQ=255)
                                                                                      aCTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATaaaaaa                                                               <  1:43907/149‑1 (MQ=255)
                                                                                        tGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAAct                                                             <  4:639989/149‑1 (MQ=255)
                                                                                            tAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTctgc                                                         <  4:444811/149‑1 (MQ=255)
                                                                                            tAGACAGGGATAAGCAAACATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTctgc                                                         <  2:673200/149‑1 (MQ=255)
                                                                                             aGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTctgct                                                        <  1:556743/149‑1 (MQ=255)
                                                                                               aCAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTgg                                                      >  2:590729/1‑149 (MQ=255)
                                                                                                 aGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCa                                                    <  3:209927/149‑1 (MQ=255)
                                                                                                 aGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCa                                                    >  4:198965/1‑149 (MQ=255)
                                                                                                  gggATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTATTGCGAGATGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCAt                                                   <  4:652540/149‑1 (MQ=255)
                                                                                                    gATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTc                                                 >  4:264565/1‑149 (MQ=255)
                                                                                                     aTAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTca                                                <  1:430231/149‑1 (MQ=255)
                                                                                                         gTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACaaa                                            <  4:155674/149‑1 (MQ=255)
                                                                                                          tAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAAt                                           >  2:525116/1‑149 (MQ=255)
                                                                                                          tAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAAt                                           >  2:237635/1‑149 (MQ=255)
                                                                                                           aaCCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATg                                          >  1:117283/1‑149 (MQ=255)
                                                                                                                tatGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAg                                     <  3:264565/149‑1 (MQ=255)
                                                                                                                 atGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAgg                                    <  1:525116/149‑1 (MQ=255)
                                                                                                                         gCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAActc                                                            >  1:44815/1‑117 (MQ=255)
                                                                                                                         gCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAActc                                                            <  2:44815/117‑1 (MQ=255)
                                                                                                                         gCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAAc                            >  1:562759/1‑149 (MQ=255)
                                                                                                                            aCCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGtt                         <  3:198965/149‑1 (MQ=255)
                                                                                                                              cTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTc                       <  1:288050/149‑1 (MQ=255)
                                                                                                                                   ggTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTc                       <  1:208972/144‑1 (MQ=255)
                                                                                                                                   ggTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTc                       >  2:208972/1‑144 (MQ=255)
                                                                                                                                        cGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCAcc             >  2:114143/1‑149 (MQ=255)
                                                                                                                                        cGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCAcc             <  2:117283/149‑1 (MQ=255)
                                                                                                                                          ttgttTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGt           >  4:357340/1‑149 (MQ=255)
                                                                                                                                            gttTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGa         >  4:386464/1‑149 (MQ=255)
                                                                                                                                                   gCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATAc  <  3:357340/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGTTGATTCAGACGCCGTACGGGAGTGCATGGATGTACGTTTATCAACGACCCGTCGATGGATTAAAGCTAATTGAAAGCGGCGACTGGTTAGACAGGGATAAGTAACCATATGCATACGCCACCTTCGGGTGGCGTTGTTTTTTGCGAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATAC  >  NC_000913/4448127‑4448422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: