Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,553,154 G→T 6.7% G85V (GGC→GTC)  uxuB → D‑mannonate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,553,1540GT 6.7% 210.5 / 5.5 60G85V (GGC→GTC) uxuBD‑mannonate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base T (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

CGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGC  >  NC_000913/4553007‑4553301
                                                                                                                                                   |                                                                                                                                                   
cgcACCCGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACGGCGAGTGGGGCATCTGGGAAGTTAACCTGATGCCAGGCAACCACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTTCTGTACACCGTAGTGGAAAAAGgc                                                                                                                                                    <  4:109873/149‑1 (MQ=255)
cgcACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc                                                                                                                                                    <  3:138579/149‑1 (MQ=255)
 gcACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGCAGCGGAAAAAGgcg                                                                                                                                                   <  3:682610/149‑1 (MQ=255)
   aCCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCa                                                                                                                                                 <  4:166217/149‑1 (MQ=255)
   aCCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCa                                                                                                                                                 >  3:65917/1‑149 (MQ=255)
     cAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCaga                                                                                                                                               >  2:376296/1‑149 (MQ=255)
      aGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCagag                                                                                                                                              <  3:368025/149‑1 (MQ=255)
          gttGTATACCCATCATCTGCTGGAAAGCACCGACCGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCa                                                                                                                                          <  3:480084/147‑1 (MQ=255)
                   ccATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa                                                                                                                                 >  4:556130/1‑149 (MQ=255)
                   ccATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa                                                                                                                                 >  1:742564/1‑149 (MQ=255)
                   ccATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGAGGAAAAAGGCGCAGAGAGCACCGAGCTGa                                                                                                                                 >  3:620105/1‑149 (MQ=255)
                    catcatATGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGaa                                                                                                                                >  2:578203/1‑149 (MQ=255)
                     atcatcTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGa                                                                                                                                      >  4:112908/1‑142 (MQ=255)
                     atcatcTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGa                                                                                                                                      <  3:112908/142‑1 (MQ=255)
                     atcatcTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGaaa                                                                                                                               <  3:754267/149‑1 (MQ=255)
                             ctgGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCgg                                                                                                                       >  3:53994/1‑149 (MQ=255)
                                  aaGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGa                                                                                                                  <  3:27304/149‑1 (MQ=255)
                                   aGCACCGAGAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGAt                                                                                                                 >  4:67927/1‑149 (MQ=255)
                                   aGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGAt                                                                                                                 <  4:53994/149‑1 (MQ=255)
                                           cAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAaagaag                                                                                                         >  3:531970/1‑149 (MQ=255)
                                            aGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAgc                                                                                                        <  1:452679/149‑1 (MQ=255)
                                             gCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAgcg                                                                                                       >  1:352224/1‑149 (MQ=255)
                                               gACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCt                                                                                                     <  4:88487/149‑1 (MQ=255)
                                               gACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCt                                                                                                     >  2:99209/1‑149 (MQ=255)
                                                   ggggCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAgcgc                                                                                                      <  1:653842/144‑1 (MQ=255)
                                                   ggggCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAgcgc                                                                                                      >  2:653842/1‑144 (MQ=255)
                                                    gggCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc                                                                                                                                                    <  4:439752/97‑1 (MQ=255)
                                                    gggCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc                                                                                                                                                    >  3:439752/1‑97 (MQ=255)
                                                            gCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCagaga                                                                                                                                             <  1:50666/96‑1 (MQ=255)
                                                            gCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCagaga                                                                                                                                             >  2:50666/1‑96 (MQ=255)
                                                              gAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGa                                                                                      <  2:230560/149‑1 (MQ=255)
                                                                 gTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATgg                                                                                   <  1:578203/149‑1 (MQ=255)
                                                                    aaCCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTg                                                                                >  1:203379/1‑149 (MQ=255)
                                                                    aaCCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTg                                                                                >  4:592128/1‑149 (MQ=255)
                                                                    aaCCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAAACGATGGCTg                                                                                >  3:371351/1‑149 (MQ=255)
                                                                      ccTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGTCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATc                                                                                        >  3:49241/1‑139 (MQ=255)
                                                                      ccTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGTCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATc                                                                                        <  4:49241/139‑1 (MQ=255)
                                                                      ccTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGTCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATc                                                                                        <  4:432442/139‑1 (MQ=255)
                                                                      ccTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGTCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATc                                                                                        >  3:432442/1‑139 (MQ=255)
                                                                      ccTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCg                                                                              >  4:802799/1‑149 (MQ=255)
                                                                        tGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAAATGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGaa                                                                            <  2:489941/149‑1 (MQ=255)
                                                                               aGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATtc                                                                     >  3:177600/1‑149 (MQ=255)
                                                                               aGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATtc                                                                     >  4:547600/1‑149 (MQ=255)
                                                                                  cAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCa                                                                  <  4:65917/149‑1 (MQ=255)
                                                                                   aaCGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCaa                                                                 <  3:631926/149‑1 (MQ=255)
                                                                                   aaCGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCaa                                                                 <  4:396480/149‑1 (MQ=255)
                                                                                    aCGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAc                                                                >  4:239470/1‑149 (MQ=255)
                                                                                      gACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAAcgc                                                              >  3:178655/1‑149 (MQ=255)
                                                                                                tGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGt                                                    >  3:447469/1‑149 (MQ=255)
                                                                                                 gATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGt                                                    >  2:590518/1‑148 (MQ=255)
                                                                                                 gATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGt                                                    <  1:590518/148‑1 (MQ=255)
                                                                                                      aaaaCCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTAt                                                                                                                          <  1:601672/73‑1 (MQ=255)
                                                                                                      aaaaCCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTAt                                                                                                                          >  2:601672/1‑73 (MQ=255)
                                                                                                           cTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGc                                         <  4:792837/149‑1 (MQ=255)
                                                                                                              aaaaaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGAt                                      <  3:556130/149‑1 (MQ=255)
                                                                                                               gaaaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGAtt                                     <  3:67927/148‑1 (MQ=255)
                                                                                                                 aaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCTGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCgg                                                                                             >  4:662010/1‑91 (MQ=255)
                                                                                                                 aaaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCgg                                                                                             <  3:662010/91‑1 (MQ=255)
                                                                                                                  aaCAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGtc                                  <  1:617034/149‑1 (MQ=255)
                                                                                                                    cAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGtctc                                <  2:742564/149‑1 (MQ=255)
                                                                                                                     aGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAaagaa                                                                                                          >  4:65463/1‑74 (MQ=255)
                                                                                                                     aGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAaagaa                                                                                                          <  3:65463/74‑1 (MQ=255)
                                                                                                                                        aGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAg            <  3:592128/149‑1 (MQ=255)
                                                                                                                                          cGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGc          <  2:203379/149‑1 (MQ=255)
                                                                                                                                           ggAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCt         >  3:281643/1‑149 (MQ=255)
                                                                                                                                                  ggCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTgcgc  <  3:802799/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGGCTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGC  >  NC_000913/4553007‑4553301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑