Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,641,005 G→A 5.1% A22T (GCA→ACA)  yjtD → putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,641,0050GA 5.1% 265.4 / 3.4 78A22T (GCA→ACA) yjtDputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (33/41);  new base A (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

TAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATC  >  NC_000913/4640860‑4641141
                                                                                                                                                 |                                                                                                                                        
tAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCaa                                                                                                                                       >  4:728005/1‑149 (MQ=255)
    aaTTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaa                                                                                                                                   <  4:221659/149‑1 (MQ=255)
          tAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  4:181703/149‑1 (MQ=255)
           aaaTTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATg                                                                                                                            <  2:83343/149‑1 (MQ=255)
              ttCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgggg                                                                                                                         >  1:30018/1‑149 (MQ=255)
              ttCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgggg                                                                                                                         >  1:44405/1‑149 (MQ=255)
                   aCCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGCTTATTTGATCAAGAAGAGTGGCAATATGCGTATACCGATTATTCTGGTCGCACCCGCCAGTGCAGAAACTACTTGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAg                                                                                                                    >  4:336215/1‑149 (MQ=255)
                     cgcgTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCg                                                                                                                  <  4:122059/149‑1 (MQ=255)
                     cgcgTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCg                                                                                                                  >  4:710343/1‑149 (MQ=255)
                         taataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCt                                                                                                              <  2:683823/149‑1 (MQ=255)
                         taataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCt                                                                                                              >  2:581354/1‑149 (MQ=255)
                         taataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCACAAAATATTGGGGCAGCGGCGCGGGCAATGAAATCGATGGGGTTTAGCGATCt                                                                                                              <  4:503544/149‑1 (MQ=255)
                          aataaGTTGACAGTGATCACCCGGTTCGCGGCTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTg                                                                                                             >  4:577273/1‑149 (MQ=255)
                           ataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGc                                                                                                            <  2:30018/149‑1 (MQ=255)
                            taaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCg                                                                                                           >  1:187714/1‑149 (MQ=255)
                              aGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             >  2:292131/1‑129 (MQ=255)
                              aGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             >  4:248701/1‑129 (MQ=255)
                              aGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  3:248701/129‑1 (MQ=255)
                              aGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  1:292131/129‑1 (MQ=255)
                               gTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGAt                                                                                                        <  4:202339/149‑1 (MQ=255)
                                  gACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGt                                                                                                     <  3:604206/149‑1 (MQ=255)
                                    cAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCg                                                                                                   <  2:187714/149‑1 (MQ=255)
                                     aGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGa                                                                                                  <  1:696022/149‑1 (MQ=255)
                                       tGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATa                                                                                                <  1:576464/149‑1 (MQ=255)
                                       tGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATa                                                                                                >  4:67398/1‑149 (MQ=255)
                                        gATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGa                                                                                                                 <  3:288849/131‑1 (MQ=255)
                                        gATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGa                                                                                                                 >  4:288849/1‑131 (MQ=255)
                                         aTCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             >  4:313117/1‑118 (MQ=255)
                                         aTCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  3:313117/118‑1 (MQ=255)
                                          tCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTc                                                                                             >  3:230163/1‑149 (MQ=255)
                                              ccGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  2:778315/113‑1 (MQ=255)
                                              ccGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             >  1:778315/1‑113 (MQ=255)
                                                ggTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGcac                                                                                       >  3:394965/1‑149 (MQ=255)
                                                  ttCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCacac                                                                                     >  1:310300/1‑149 (MQ=255)
                                                    cgcgGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCt                                                                                   <  4:691556/149‑1 (MQ=255)
                                                       ggTTATTTGATCAAGAAGTGTGGCAATATGCGTATAACGATTATTCGGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                <  3:464128/149‑1 (MQ=255)
                                                                aTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAc                                                                       >  1:612699/1‑149 (MQ=255)
                                                                 tCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAcc                                                                      >  3:347368/1‑149 (MQ=255)
                                                                   aagaagAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCg                                                                    >  2:610885/1‑149 (MQ=255)
                                                                     gaagaGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTc                                                                                                    <  2:777544/115‑1 (MQ=255)
                                                                     gaagaGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTc                                                                                                    >  1:777544/1‑115 (MQ=255)
                                                                     gaagaGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCt                                                                  <  3:710343/149‑1 (MQ=255)
                                                                                atatGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGa                                                                                                         <  3:395020/99‑1 (MQ=255)
                                                                                atatGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGa                                                                                                         >  4:395020/1‑99 (MQ=255)
                                                                                  atGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGAt                                                                                                        <  4:771547/98‑1 (MQ=255)
                                                                                  atGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGAt                                                                                                        >  3:771547/1‑98 (MQ=255)
                                                                                    gCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGAt                                                   <  3:728005/149‑1 (MQ=255)
                                                                                      gTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGcaca                                                        <  3:396815/142‑1 (MQ=255)
                                                                                      gTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGcaca                                                        >  4:396815/1‑142 (MQ=255)
                                                                                        ataACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTgg                                               <  1:621007/149‑1 (MQ=255)
                                                                                        ataACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTgg                                               <  2:44405/149‑1 (MQ=255)
                                                                                         taACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGt                                              >  4:173847/1‑149 (MQ=255)
                                                                                         taACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGt                                              <  4:230163/149‑1 (MQ=255)
                                                                                          aaCGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTg                                             <  4:394965/149‑1 (MQ=255)
                                                                                             gATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             >  1:579219/1‑66 (MQ=255)
                                                                                             gATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                                                             <  2:579219/66‑1 (MQ=255)
                                                                                              attattCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGatat                                         <  2:537560/149‑1 (MQ=255)
                                                                                               ttattCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                >  4:796816/1‑109 (MQ=255)
                                                                                               ttattCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                <  3:796816/109‑1 (MQ=255)
                                                                                                  ttCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATattatt                                     <  3:336215/149‑1 (MQ=255)
                                                                                                     tGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGat                                  >  1:370995/1‑149 (MQ=255)
                                                                                                       gTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGACAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                     <  4:698204/112‑1 (MQ=255)
                                                                                                       gTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGACAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                     <  4:679366/112‑1 (MQ=255)
                                                                                                       gTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGACAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                     >  3:679366/1‑112 (MQ=255)
                                                                                                       gTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGACAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                     >  3:698204/1‑112 (MQ=255)
                                                                                                         cGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGataata                              <  2:612699/149‑1 (MQ=255)
                                                                                                              ccGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTaaa                         >  1:408022/1‑149 (MQ=255)
                                                                                                               cGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTaaa                         <  4:602641/148‑1 (MQ=255)
                                                                                                               cGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTaaa                         >  3:602641/1‑148 (MQ=255)
                                                                                                                  cAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATattatt                                     >  4:355173/1‑133 (MQ=255)
                                                                                                                  cAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATattatt                                     <  3:355173/133‑1 (MQ=255)
                                                                                                                  cAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGttt                     >  1:499757/1‑149 (MQ=255)
                                                                                                                     agCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACGCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTcc                  <  3:67398/149‑1 (MQ=255)
                                                                                                                        aGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGa               <  4:347368/149‑1 (MQ=255)
                                                                                                                         gAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                <  4:724881/83‑1 (MQ=255)
                                                                                                                         gAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGa                                                                                >  3:724881/1‑83 (MQ=255)
                                                                                                                               atTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGc        <  2:370995/149‑1 (MQ=255)
                                                                                                                                     gCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATc  <  2:310300/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
TAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATC  >  NC_000913/4640860‑4641141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑