Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,094 G→C 10.2% intergenic (+61/+167) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,0940GC10.2% 165.8 / 10.8 49intergenic (+61/+167)glxK/allEglycerate kinase II/S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base C (2/3);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

AGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAA  >  NC_000913/542946‑543238
                                                                                                                                                    |                                                                                                                                                
aGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATg                                                                                                                                                  <  2:514835/149‑1 (MQ=255)
        ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                          <  4:90248/149‑1 (MQ=255)
        ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                          <  3:493510/149‑1 (MQ=255)
        ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                          <  4:592236/149‑1 (MQ=255)
        ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCAGGa                                                                                                                                          <  1:561424/149‑1 (MQ=255)
           gCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                          >  1:76583/1‑146 (MQ=255)
           gCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                          <  2:76583/146‑1 (MQ=255)
               tGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGc                                                                                                                                              >  1:506941/1‑138 (MQ=255)
               tGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGc                                                                                                                                              <  2:506941/138‑1 (MQ=255)
                gAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCata                                                                                                                                  <  3:185412/149‑1 (MQ=255)
                   aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                               >  1:757179/1‑149 (MQ=255)
                   aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                               >  3:375651/1‑149 (MQ=255)
                     cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcctt                                                                                                                             >  2:286446/1‑149 (MQ=255)
                     cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAg                                                                                                                                         <  1:682520/137‑1 (MQ=255)
                     cAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAg                                                                                                                                         >  2:682520/1‑137 (MQ=255)
                          tctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGa                                                                                                                        >  2:704485/1‑149 (MQ=255)
                                  aGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATcgcg                                                                                                                >  4:766853/1‑149 (MQ=255)
                                     gcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                            >  3:103590/1‑118 (MQ=255)
                                     gcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                            <  4:103590/118‑1 (MQ=255)
                                         gAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTc                                                                                                         >  1:712868/1‑149 (MQ=255)
                                           aaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAAt                                                                                                                    <  2:322713/136‑1 (MQ=255)
                                           aaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAAt                                                                                                                    >  1:322713/1‑136 (MQ=255)
                                           aaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCta                                                                                                       <  3:766853/149‑1 (MQ=255)
                                             tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtata                                                                                                     >  3:97432/1‑149 (MQ=255)
                                                    tGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAGTa                                                                                                                                       >  3:658557/1‑108 (MQ=255)
                                                    tGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAGTa                                                                                                                                       <  4:658557/108‑1 (MQ=255)
                                                        ccATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAgaga                                                                                          <  4:375651/149‑1 (MQ=255)
                                                          aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                        <  1:704485/149‑1 (MQ=255)
                                                              aaaTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATg                                                                                                                                                  <  4:413185/87‑1 (MQ=255)
                                                              aaaTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATg                                                                                                                                                  >  3:413185/1‑87 (MQ=255)
                                                                    gTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                            >  2:493322/1‑87 (MQ=255)
                                                                    gTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                            <  1:493322/87‑1 (MQ=255)
                                                                         ggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaa                                                                         >  3:319183/1‑149 (MQ=255)
                                                                              tAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa                                                                    <  4:97432/149‑1 (MQ=255)
                                                                              tAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa                                                                    >  1:359807/1‑149 (MQ=255)
                                                                                     tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATc                                                             <  2:697888/149‑1 (MQ=255)
                                                                                            tACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAAt                                                      <  4:319183/149‑1 (MQ=255)
                                                                                              ccTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGt                                                    <  2:712868/149‑1 (MQ=255)
                                                                                                tttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTa                                                  >  3:13006/1‑149 (MQ=255)
                                                                                                         gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaagc                                                                  >  2:607264/1‑124 (MQ=255)
                                                                                                         gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaagc                                                                  <  1:607264/124‑1 (MQ=255)
                                                                                                         gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaaa                                          <  3:597308/148‑1 (MQ=255)
                                                                                                         gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaaa                                          >  4:597308/1‑148 (MQ=255)
                                                                                                               aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                   <  1:286446/149‑1 (MQ=255)
                                                                                                               aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                   >  4:737421/1‑149 (MQ=255)
                                                                                                                     atCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAgg                                                          <  1:198879/120‑1 (MQ=255)
                                                                                                                     atCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAgg                                                          >  2:198879/1‑120 (MQ=255)
                                                                                                                          aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                        <  3:737421/149‑1 (MQ=255)
                                                                                                                                gTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaaa                  <  2:359807/149‑1 (MQ=255)
                                                                                                                                     gTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa             >  4:727688/1‑149 (MQ=255)
                                                                                                                                              ccGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaa    <  3:727688/149‑1 (MQ=255)
                                                                                                                                                ggATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaaaa  >  4:38859/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAA  >  NC_000913/542946‑543238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: