Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 603,395 C→G 5.7% intergenic (+60/+21) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913603,3950CG 5.7% 320.8 / 8.9 88intergenic (+60/+21)pheP/ybdGphenylalanine transporter/mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (43/40);  new base G (3/2);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

GATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603248‑603540
                                                                                                                                                   |                                                                                                                                                 
gatggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCg                                                                                                                                                  >  4:353647/1‑149 (MQ=255)
  tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                <  4:541311/149‑1 (MQ=255)
  tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                <  4:586988/149‑1 (MQ=255)
  tggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGAt                                                                                                                                                <  1:689957/149‑1 (MQ=255)
   ggatgATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATg                                                                                                                                               >  4:125517/1‑149 (MQ=255)
    gatgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATga                                                                                                                                              <  2:126726/149‑1 (MQ=255)
      tgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                            >  4:790367/1‑149 (MQ=255)
      tgatATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATgaga                                                                                                                                            <  1:576809/149‑1 (MQ=255)
       gatATGCGCTTGTCAGTGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCGAATGGATGagag                                                                                                                                           <  3:85699/149‑1 (MQ=255)
                ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  >  3:254113/1‑149 (MQ=255)
                ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  <  3:125517/149‑1 (MQ=255)
                ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  >  2:756165/1‑149 (MQ=255)
                ttGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  >  1:560921/1‑149 (MQ=255)
                   tCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTaa                                                                                                                               <  3:790367/149‑1 (MQ=255)
                      gCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCAAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                          >  2:470856/1‑135 (MQ=255)
                      gCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCAAAACCGCTCTCATCGAATGGATGAGAGc                                                                                                                                          <  1:470856/135‑1 (MQ=255)
                       cGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTAc                                                                                                                           <  1:756165/149‑1 (MQ=255)
                       cGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTAc                                                                                                                           >  2:57298/1‑149 (MQ=255)
                         aTCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTg                                                                                                                         >  2:625670/1‑149 (MQ=255)
                          tCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGc                                                                                                                        >  3:112205/1‑149 (MQ=255)
                                tgcCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                  <  2:552433/149‑1 (MQ=255)
                                    ggTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCAc                                                                                                              <  1:57298/149‑1 (MQ=255)
                                         ggATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGcc                                                                                                         >  1:58651/1‑149 (MQ=255)
                                          gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGCCAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                  >  4:463754/1‑139 (MQ=255)
                                          gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCCACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                  <  3:463754/139‑1 (MQ=255)
                                          gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCa                                                                                                        <  1:443425/149‑1 (MQ=255)
                                          gATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCa                                                                                                        >  2:797060/1‑149 (MQ=255)
                                           aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCag                                                                                                       >  3:787671/1‑149 (MQ=255)
                                           aTTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATCACTGCTTAAATGCACTCGCCag                                                                                                       >  1:817413/1‑149 (MQ=255)
                                                aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGANc                                                                                                  <  3:79017/149‑1 (MQ=255)
                                                aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGc                                                                                                  <  4:398947/149‑1 (MQ=255)
                                                aTTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGc                                                                                                  <  3:584434/149‑1 (MQ=255)
                                                 ttCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCg                                                                                                 >  4:680615/1‑149 (MQ=255)
                                                  tCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  >  3:391781/1‑115 (MQ=255)
                                                  tCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGtttttt                                                                                                                                  <  4:391781/115‑1 (MQ=255)
                                                   ccTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                  <  3:298861/130‑1 (MQ=255)
                                                   ccTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAAt                                                                                                                  >  4:298861/1‑130 (MQ=255)
                                                          aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGATCGAATATCAtt                                                                                        <  3:517317/149‑1 (MQ=255)
                                                          aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                        <  4:787671/149‑1 (MQ=255)
                                                          aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                        <  2:817413/149‑1 (MQ=255)
                                                          aTGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCAtt                                                                                        <  4:279087/149‑1 (MQ=255)
                                                                tttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGt                                                                                  >  4:385496/1‑149 (MQ=255)
                                                                tttAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGt                                                                                  >  1:743660/1‑149 (MQ=255)
                                                                   aaaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTgg                                                                               >  3:703680/1‑149 (MQ=255)
                                                                    aaaCGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGc                                                                              >  4:645823/1‑149 (MQ=255)
                                                                          tGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACtga                                                                        <  2:130707/149‑1 (MQ=255)
                                                                                    aTAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCa                                                              <  1:797060/149‑1 (MQ=255)
                                                                                            ttCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAAc                                                      >  2:670191/1‑149 (MQ=255)
                                                                                               aCGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAActct                                                   >  4:620610/1‑149 (MQ=255)
                                                                                                cGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCtt                                                  <  3:353647/149‑1 (MQ=255)
                                                                                                cGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCtt                                                  <  3:680615/149‑1 (MQ=255)
                                                                                                 gCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                 <  2:560921/149‑1 (MQ=255)
                                                                                                 gCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                 <  4:254113/149‑1 (MQ=255)
                                                                                                  cTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                 <  3:48036/148‑1 (MQ=255)
                                                                                                  cTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTc                                                 >  4:48036/1‑148 (MQ=255)
                                                                                                          gACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGc                                        >  2:775519/1‑149 (MQ=255)
                                                                                                            cAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCaa                                      <  1:625670/149‑1 (MQ=255)
                                                                                                             aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAg                                                                   <  2:628978/119‑1 (MQ=255)
                                                                                                             aaaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAg                                                                   >  1:628978/1‑119 (MQ=255)
                                                                                                              aaaCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAat                                    >  4:420265/1‑149 (MQ=255)
                                                                                                                aCGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAatat                                  <  1:670191/149‑1 (MQ=255)
                                                                                                                  gATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgt                                >  2:77592/1‑149 (MQ=255)
                                                                                                                  gATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATAtgt                                <  1:775519/149‑1 (MQ=255)
                                                                                                                        aaCCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGa                          >  4:624352/1‑149 (MQ=255)
                                                                                                                          ccATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAat                        >  4:652253/1‑149 (MQ=255)
                                                                                                                           cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       <  3:645823/149‑1 (MQ=255)
                                                                                                                           cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       <  3:385496/149‑1 (MQ=255)
                                                                                                                           cATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       >  2:255615/1‑149 (MQ=255)
                                                                                                                                gAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                  <  4:703680/149‑1 (MQ=255)
                                                                                                                                     cGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGTATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTg             >  4:462213/1‑149 (MQ=255)
                                                                                                                                     cGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTg             >  4:486203/1‑149 (MQ=255)
                                                                                                                                     cGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTg             >  2:561957/1‑149 (MQ=255)
                                                                                                                                      gCTCTCATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa            >  1:684907/1‑149 (MQ=255)
                                                                                                                                      gCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                  <  3:382668/143‑1 (MQ=255)
                                                                                                                                      gCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCa                  >  4:382668/1‑143 (MQ=255)
                                                                                                                                      gCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa            >  4:438773/1‑149 (MQ=255)
                                                                                                                                      gCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGa            >  2:171696/1‑149 (MQ=255)
                                                                                                                                        tctcATCGAATGGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAAt          >  3:180944/1‑149 (MQ=255)
                                                                                                                                            aTCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCtt      <  1:255615/149‑1 (MQ=255)
                                                                                                                                             tCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCttt     >  3:131365/1‑149 (MQ=255)
                                                                                                                                                aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat  >  3:23987/1‑149 (MQ=255)
                                                                                                                                                aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCat  >  2:289017/1‑149 (MQ=255)
                                                                                                                                                aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCa   <  1:286623/148‑1 (MQ=255)
                                                                                                                                                aTTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCa   >  2:286623/1‑148 (MQ=255)
                                                                                                                                                 ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa                         <  4:48303/125‑1 (MQ=255)
                                                                                                                                                 ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGaa                         >  3:48303/1‑125 (MQ=255)
                                                                                                                                                 ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       <  4:310138/127‑1 (MQ=255)
                                                                                                                                                 ttCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAata                       >  3:310138/1‑127 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GATGGATGATATGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAAAACGATGTCAACCATCCGAAACCGCTCTCATCCATTCGATGAGAGCGGTTTTTTTAATTACTGCTTAAATGCACCCGCCAGAGAGCGAATATCATTGCCGGTTGGCGACTGATGAAGTCGCAGACCAAACTCTTCGACAATCGCAAATATGTGATCGAATATATCAGCCTGAATGCTTTCAT  >  NC_000913/603248‑603540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: