Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 725,276 G→A 7.3% Y587Y (TAC→TAT kdpB ← potassium translocating ATPase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913725,2760GA 7.3% 355.3 / 12.5 109Y587Y (TAC→TATkdpBpotassium translocating ATPase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (44/57);  new base A (3/5);  total (47/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 109 total aligned reads displayed.

ACGCCAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGT  >  NC_000913/725131‑725422
                                                                                                                                                 |                                                                                                                                                  
aCGCCAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTAt                                                                                                                                                 >  3:423391/1‑149 (MQ=255)
aCGCCAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTAt                                                                                                                                                 >  1:286878/1‑149 (MQ=255)
   ccAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTc                                                                                                                                              <  1:130337/149‑1 (MQ=255)
   ccAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTc                                                                                                                                              <  2:345276/149‑1 (MQ=255)
   ccAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTc                                                                                                                                              >  1:199991/1‑149 (MQ=255)
     aGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGc                                                                                                                                            <  1:308237/149‑1 (MQ=255)
     aGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGc                                                                                                                                            <  1:460926/149‑1 (MQ=255)
     aGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGc                                                                                                                                            <  1:507313/149‑1 (MQ=255)
      ggggAATCAAAAAGACGATACTCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTCCGcc                                                                                                                                           <  2:298682/149‑1 (MQ=255)
      ggggAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCTGAATAATGGCGAAGTATTTCGcc                                                                                                                                           >  2:294414/1‑149 (MQ=255)
      ggggAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGcc                                                                                                                                           <  4:402601/149‑1 (MQ=255)
       gggAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCca                                                                                                                                          >  2:757035/1‑149 (MQ=255)
       gggAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTCGCca                                                                                                                                          <  1:30766/149‑1 (MQ=255)
        ggAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTCGCcat                                                                                                                                         <  2:199991/149‑2 (MQ=255)
         gAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAATACTTCGCcatt                                                                                                                                        <  1:428253/149‑3 (MQ=255)
          aaTCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACAt                                                                                                                                       >  3:750662/1‑149 (MQ=255)
               aaaaGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTg                                                                                                                                  <  4:709473/149‑1 (MQ=255)
               aaaaGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTg                                                                                                                                  <  1:107513/149‑1 (MQ=255)
               aaaaGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTg                                                                                                                                  <  1:739014/149‑1 (MQ=255)
                 aaGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGc                                                                                                                                >  3:586409/1‑149 (MQ=255)
                 aaGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGc                                                                                                                                >  3:375966/1‑149 (MQ=255)
                   gACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGtt                                                                                                                                   <  4:544651/144‑1 (MQ=255)
                   gACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCaa                                                                                                                              <  4:750662/149‑1 (MQ=255)
                    aCGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAAt                                                                                                                             >  4:223503/1‑149 (MQ=255)
                      gATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGc                                                                                                                           <  2:419568/149‑1 (MQ=255)
                      gATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGc                                                                                                                           <  4:676731/149‑1 (MQ=255)
                      gATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGc                                                                                                                           >  3:270506/1‑149 (MQ=255)
                       aTAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCt                                                                                                                          <  1:91704/149‑1 (MQ=255)
                           tCAAGGCGTTGAAAATCACCGCACTGAGGCTTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAg                                                                                                                      >  2:241766/1‑149 (MQ=255)
                           tCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAg                                                                                                                      >  2:241765/1‑149 (MQ=255)
                            cAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAgg                                                                                                                     >  3:603375/1‑149 (MQ=255)
                             aaGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAggt                                                                                                                    >  4:640483/1‑149 (MQ=255)
                              aGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAggtg                                                                                                                   <  3:155116/149‑1 (MQ=255)
                              aGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAggtg                                                                                                                   <  1:56155/149‑1 (MQ=255)
                              aGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAggtg                                                                                                                   <  2:583309/149‑1 (MQ=255)
                              aGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAggtg                                                                                                                   <  1:329253/149‑1 (MQ=255)
                               ggCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTg                                                                                                                                  >  4:138389/1‑133 (MQ=255)
                               ggCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTg                                                                                                                                  <  3:138389/133‑1 (MQ=255)
                                  gTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGtt                                                                                                                                   <  3:381253/129‑1 (MQ=255)
                                  gTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGtt                                                                                                                                   >  4:381253/1‑129 (MQ=255)
                                   ttGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAg                                                                                                              >  3:727198/1‑149 (MQ=255)
                                      aaaaTCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGa                                                                                                           <  4:490024/149‑1 (MQ=255)
                                            aCCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACg                                                                                                     >  1:431424/1‑149 (MQ=255)
                                            aCCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACg                                                                                                     >  2:754486/1‑149 (MQ=255)
                                            aCCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACg                                                                                                     >  4:11501/1‑149 (MQ=255)
                                                   tGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGtcatc                                                                                              >  2:543428/1‑149 (MQ=255)
                                                       gATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCa                                                                                          >  3:364326/1‑149 (MQ=255)
                                                          tGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCt                                                                                       <  3:640483/149‑1 (MQ=255)
                                                          tGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCt                                                                                       <  3:183206/149‑1 (MQ=255)
                                                             ggAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGtt                                                                                    >  1:307725/1‑149 (MQ=255)
                                                             ggAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGtt                                                                                    >  3:134830/1‑149 (MQ=255)
                                                              gAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt                                                                                   <  4:375966/149‑1 (MQ=255)
                                                                   gggCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt                                                                                   <  3:5290/144‑1 (MQ=255)
                                                                   gggCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt                                                                                   >  4:5290/1‑144 (MQ=255)
                                                                   gggCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCaa                                                                              >  2:744262/1‑149 (MQ=255)
                                                                   gggCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCaa                                                                              <  3:625124/149‑1 (MQ=255)
                                                                    ggCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAAt                                                                             <  1:754486/149‑1 (MQ=255)
                                                                       gAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAAtgtg                                                                          <  4:423391/149‑1 (MQ=255)
                                                                       gAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGACACGGGTCATCAGCATCTGTTTGCCAAtgtg                                                                          <  2:286878/149‑1 (MQ=255)
                                                                          tGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGcac                                                                       <  4:798500/149‑1 (MQ=255)
                                                                              ggCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt                                                                                   <  2:466866/133‑1 (MQ=255)
                                                                              ggCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGttt                                                                                   >  1:466866/1‑133 (MQ=255)
                                                                                 acaTGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGc                                                                                                                           >  3:32160/1‑90 (MQ=255)
                                                                                 acaTGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGc                                                                                                                           <  4:32160/90‑1 (MQ=255)
                                                                                   atgatgTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGAt                                                              <  3:774675/149‑1 (MQ=255)
                                                                                     gatgTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCa                                                            >  2:194181/1‑149 (MQ=255)
                                                                                      atgTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGc                                                                         >  1:681854/1‑135 (MQ=255)
                                                                                       acgtatccgcAGTTAAATGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAAc                                                          >  2:680406/8‑149 (MQ=255)
                                                                                          tCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTg                                                       <  4:364326/149‑1 (MQ=255)
                                                                                           cAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTgg                                                      <  3:289872/149‑1 (MQ=255)
                                                                                               gcATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCgg                                                  <  4:603375/149‑1 (MQ=255)
                                                                                                    tAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTag                                             <  3:31301/149‑1 (MQ=255)
                                                                                                     aaCTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTTCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTaga                                            <  3:497072/149‑1 (MQ=255)
                                                                                                     aaCTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTaga                                            <  4:288717/149‑1 (MQ=255)
                                                                                                      aCTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTaga                                            <  3:413955/148‑1 (MQ=255)
                                                                                                      aCTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTaga                                            >  4:413955/1‑148 (MQ=255)
                                                                                                        tGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTc                                         <  4:586409/149‑1 (MQ=255)
                                                                                                        tGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTc                                         >  4:417072/1‑149 (MQ=255)
                                                                                                          cGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCga                                       <  2:145959/149‑1 (MQ=255)
                                                                                                          cGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCga                                       <  4:727198/149‑1 (MQ=255)
                                                                                                          cGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCga                                       <  1:680406/149‑1 (MQ=255)
                                                                                                               aCGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCg                                  <  3:223503/149‑1 (MQ=255)
                                                                                                                cGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGa                                 <  3:11501/149‑1 (MQ=255)
                                                                                                                cGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGa                                 <  1:543428/149‑1 (MQ=255)
                                                                                                                 gTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGaa                                                                                                                       >  4:303988/1‑62 (MQ=255)
                                                                                                                  ttGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGAcc                               >  1:249238/1‑149 (MQ=255)
                                                                                                                         gAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGc                        >  3:90480/1‑149 (MQ=255)
                                                                                                                                  cgGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCtt               <  1:757035/149‑1 (MQ=255)
                                                                                                                                  cgGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCtt               <  1:241765/149‑1 (MQ=255)
                                                                                                                                  cgGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCtt               <  1:241766/149‑1 (MQ=255)
                                                                                                                                   aacgatgtGGCGAAATACTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCttt              >  3:384680/8‑149 (MQ=255)
                                                                                                                                     aataatGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcg            >  2:223441/1‑149 (MQ=255)
                                                                                                                                      ataatGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgc           <  1:223441/149‑1 (MQ=255)
                                                                                                                                        aatGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACAATATTGCCCGCCTCTTTcgccg         >  3:455674/1‑149 (MQ=255)
                                                                                                                                         atGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgccgc        <  2:307725/149‑1 (MQ=255)
                                                                                                                                         atGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgcc          <  2:618444/147‑1 (MQ=255)
                                                                                                                                         atGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTcgcc          >  1:618444/1‑147 (MQ=255)
                                                                                                                                           ggCGAAATACTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTc                                         <  3:99948/114‑1 (MQ=255)
                                                                                                                                            gCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTg     >  2:175854/1‑149 (MQ=255)
                                                                                                                                              gAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTggg   >  1:72106/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACGCCAGGGGAATCAAAAAGACGATAATCAAGGCGTTGAAAATCACCGCACTGAGGATTGCGGAGTCGGGCGAATGCAGGCACATGATGTTCAGCGCATTTAACTGCGGATACGTTGCCGCGAATGCCGCCGGAATAATGGCGAAGTATTTCGCCACATCGTTGGCAATGCTGAAGGTGGTCAGCGAGCCACGGGTCATCAGCATCTGTTTGCCAATGTGCACCACCTCGATCAACTTGGTCGGGTTAGAGTCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGT  >  NC_000913/725131‑725422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: