Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA99.2% 429.6 / NA 122S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (75/46);  minor base T (0/1);  total (75/47)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145040‑2145584
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
aGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCat                                                                                                                                                                                                                                                                               >  2:152036/1‑278 (MQ=255)
              ggTGTGCCGATCCTGACGGTGTTGCATATGAATGTGGGAGACAACGGACAATTAATGGAGGACATGTACAGTGCCGACACCTCACGTTCCGAGCTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGc                                                                                                                                                                                                                                                                  <  2:16084/277‑1 (MQ=255)
                  ttCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAt                                                                                                                                                                                                                                                               <  1:136297/275‑1 (MQ=255)
                     cGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGtt                                                                                                                                                                                                                                            >  3:302307/1‑275 (MQ=255)
                      gCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCGGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCACTATTTGTTCGGTTCTGGGGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGCGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCa                                                                                                                                                                                                                                                        >  4:513900/1‑278 (MQ=255)
                                gtgttttgaatTACTTTTAACGGAAAAGGAACGTGAGTGGAGAACATTTACAGTGGCGCCACCAAACGTTCCGCGTTCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTGCGACGTGGATTACAGTATTACGCCGATCAGTACTGTGGACGGCATCAATATTTGTTCGGTTCTGGTGATTCAGGTCGTTACCGTATCACGCAAAATGCTGCGCTCGCTGAGGTACAGCGCCTACCATGAGGCACTGACGTATCTCGCCAACCgcgc                                                                                                                                                                                                                                                 <  3:556230/266‑1 (MQ=255)
                                 tgtTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACACTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAAGATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCCACAGCCGAGCTACAGCGCCTACCATGACGACATGACGCAGCTCGACCACCGCGCCCGt                                                                                                                                                                                                                                             >  4:158033/1‑278 (MQ=255)
                                            aCTTTTGGCGACAACGGACCATTAATGGAGAACATTTAAAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgaga                                                                                                                                                                                                                                      <  1:604554/274‑1 (MQ=255)
                                                  ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACt                                                                                                                                                                                                                               >  1:123838/1‑275 (MQ=255)
                                                  ggCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCg                                                                                                                                                                                                                            >  2:83956/1‑278 (MQ=255)
                                                     gACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGc                                                                                                                                                                                                                             <  1:158004/274‑1 (MQ=255)
                                                       cAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTa                                                                                                                                                                                                                          <  1:72318/275‑1 (MQ=255)
                                                                aatAATGGGGAACATTTGCAGTCCCGAGACCTCCCGTTCCGCGTTCGAGAAAGATGTGGGGTTGCACGGTCGGGGGGGCGGCAGGTACGACGTGCATTACAGTCTTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTTGTGCTTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACGGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCtgctgc                                                                                                                                                                                                                 <  3:528137/273‑1 (MQ=255)
                                                                  taaggggggaCAGTTACCGTGCTGAACCCTGACGTTCCGAGATGGATCATGATGTGGTGTTGCACTGGCGGAACGGCGGCAGTTACGGCGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  4:476418/269‑1 (MQ=255)
                                                                     tGGCGAACATTGACAGGCCCGACAACCCACGTCGCGCGAGCGAGCAAGGTGTGGTGTTGCACGGTCGGAGCGGCGGCAGTTACTACGTGCAGTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGTTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  3:248766/275‑1 (MQ=255)
                                                                        ggaAAATCTACTGTGCCGACACCTCCCGTTCCGCGGTCGAGGAAGATGGGGTGTTGCCCGGTCGGAGCGGCGGCAGTTACGAAGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       <  4:152036/276‑1 (MQ=255)
                                                                        agaACATTTACAGGGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       <  2:298009/277‑1 (MQ=255)
                                                                          aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGCCGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       >  1:593812/1‑275 (MQ=255)
                                                                          aaCATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                       >  1:99195/1‑275 (MQ=255)
                                                                            cATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  1:302307/274‑1 (MQ=255)
                                                                            cATTCACATTGCCGGCGCCCAGCGTTCCGCGACAGAGCAAGATGTGGTGTGGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  2:4005/278‑1 (MQ=255)
                                                                               ttACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                  <  1:197223/275‑1 (MQ=255)
                                                                                 aCAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCCCCAGTTTTGAGAAACAACTGCGTAACCTGCTGCAAACGGTAAACATTac                                                                                                                                                                                                 >  1:277599/1‑274 (MQ=255)
                                                                                    gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTaca                                                                                                                                                                                                >  1:409583/1‑272 (MQ=255)
                                                                                    gTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGGTTTGAGAAACAAATGCGTATCCTGCTGCAAACGGTAAACAGTaca                                                                                                                                                                                                >  1:245721/1‑272 (MQ=255)
                                                                                       ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGACACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGa                                                                                                                                                                                       >  2:380887/1‑278 (MQ=255)
                                                                                        cGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGCTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                      <  2:488110/278‑1 (MQ=255)
                                                                                             ccTCCCGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 <  2:96242/278‑1 (MQ=255)
                                                                                               tCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAACCAGTACACATCAGCGACATcccc                                                                                                                                                                                >  2:118580/1‑277 (MQ=255)
                                                                                                cACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 >  1:586621/1‑275 (MQ=255)
                                                                                                    ttCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGcc                                                                                                                                                                                 <  1:50636/271‑1 (MQ=255)
                                                                                                       cgcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATAATGCGCCAGCTGAGCTACAGGGCCTACCATTATGCACTGACGCATCCATCAAACCGCCCCAGCTTTGAGAAACAAATGGGCATCCTGCTGCAAAAGGTAACAGTACAAAGCAGAGACATGCACAGGTGtttt                                                                                                                                                                       >  2:366024/1‑276 (MQ=255)
                                                                                                        gcgATCGGGGAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  2:271947/278‑1 (MQ=255)
                                                                                                        gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAt                                                                                                                                                                      <  2:47385/278‑1 (MQ=255)
                                                                                                                   agagGTGGTGGTGGACGGTCGGGGCGGCGGAAGTTACGAAGTGCATGACAGGATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTGCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                            <  4:445121/277‑1 (MQ=255)
                                                                                                                          gtgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATcc                                                                                                                                                                                                                       >  2:146222/1‑211 (MQ=255)
                                                                                                                           tgtTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTTAGAAACAACTGCGTATCCTGCTGCAAACGGGAAACAGTACTCATCAGCTGCATGCCCTGGTGTTTAGTGATCGTTATCGCttt                                                                                                                                                      >  3:76150/1‑275 (MQ=255)
                                                                                                                             ttGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCCCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTAACCTGCTGAACAATGTAAACAGTCAACATCAGCGACATCCCCTGGTGTTTATCGAACTTGCACCCTTTaa                                                                                                                                                    >  3:493230/1‑275 (MQ=255)
                                                                                                                             ttGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTATGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGAACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  2:191180/277‑1 (MQ=255)
                                                                                                                              tGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCAAGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCCTCTCGCCAACCGCGCCAGTCTTTAGAACCAACTGCGTATCCTGCTTCAAACGGTAAACAGTACACATCAGCGACATGCCCTGATGTTTATCGTTCTTGATCGCTTTat                                                                                                                                                    >  1:556908/1‑273 (MQ=255)
                                                                                                                                cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTTTTTATCGATCTTGATCGCTTTAAAGc                                                                                                                                                 >  1:45745/1‑275 (MQ=255)
                                                                                                                                cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCgg                                                                                                                                               >  2:494712/1‑277 (MQ=255)
                                                                                                                                agcggttggAGCGGCGGCAGTTACGACGTGCATTACAGTAGTACGCCGGTAAGCACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                  <  1:132275/267‑1 (MQ=255)
                                                                                                                                     tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACcgcg                                                                                                                                                                                                                                                  >  4:465673/1‑173 (MQ=255)
                                                                                                                                      cGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCgcgc                                                                                                                                                                                                                                                 <  2:465673/173‑1 (MQ=255)
                                                                                                                                          gcggagGCACTTACGACGTGCATTACAGTATTACGCCGATAAGTACTCTGGACGGCCGCAATTGTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCGGCTGAGCTACTGCGCCTACCATGAGGCACTTACGCATCTCGCCACCCGCACAAGTTTTGAGAAACAACTGCGTAGCCTGCCGCAGACGGACCACATTTCACATCACCGACATGCCCTGGTTTTTACCGGTCTTGTTCGCTTTAAAGCGGTaaactac                                                                                                                                       >  3:607933/1‑271 (MQ=255)
                                                                                                                                               ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTTATCGCTTTAAACCGGTGAATGAGTGCGCCgg                                                                                                                               >  2:438582/1‑278 (MQ=255)
                                                                                                                                               ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATATCGCGAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGAGACATGCACTGGTGTGTATCGACCTTGATCGCTTTAAAGCGGTGAATGAAAgcgc                                                                                                                                  >  1:418193/1‑275 (MQ=255)
                                                                                                                                                              cATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   >  1:442097/1‑275 (MQ=255)
                                                                                                                                                              aattcaaGTATTACGCCGTGAAGTACGCTGGCCGGCAGCAAGATTGGTTCGGTTCTGAGGATTCAGGACGTGACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCggg                                                                                                                   <  3:161319/269‑1 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGACATGCCCTGGTGTTTCTCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGACGGGCCTGCGGCGGGTGAGGCTTtg                                                                                                         >  3:327608/1‑275 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGATATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCCTGCGGCGGGTGACGCTTtgctg                                                                                                     >  2:109923/1‑278 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgctg                                                                                                     >  2:468110/1‑278 (MQ=255)
                                                                                                                                                                        ttACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtg                                                                                                         >  1:40459/1‑275 (MQ=255)
                                                                                                                                                                          aCGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTg                                                                                                                                                                                                                                         <  1:57876/145‑1 (MQ=255)
                                                                                                                                                                           cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAACCGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGAGCGCTATGCTgcgc                                                                                                  >  4:473526/1‑278 (MQ=255)
                                                                                                                                                                           cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTtgctg                                                                                                     >  3:353157/1‑275 (MQ=255)
                                                                                                                                                                              cGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGac                                                                                               >  2:446454/1‑277 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCat                                                                                                                                                                                                                                                                               >  4:406388/1‑102 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                >  1:332694/1‑275 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                >  1:108413/1‑275 (MQ=255)
                                                                                                                                                                                ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTCGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGTCCTGGTGTTTATCGATCTTGATCGCTTTAAAGAGGTGAATGACAGCGCCGGGACTGCGGAGGGTGACGCTTtgctggcc                                                                                                  >  3:33826/1‑270 (MQ=255)
                                                                                                                                                                                 tAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                              <  2:406388/102‑1 (MQ=255)
                                                                                                                                                                                        tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACTGCTTGTTTCGCGAACTGGcgtcgt                                                                                     >  4:514060/1‑278 (MQ=255)
                                                                                                                                                                                        tctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTAACCTGCTGCAAACGGTAAACAGTACACATCAGCGACATCCCCTGGTGTTTATCGATCATGACCGCTTTAAAGCCGTTAATGAAAGCGCCGGGAATGCCGCGGGTGACGCATTGCGTGCCGAACTGGccgcgt                                                                                     >  4:135804/1‑273 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAAc                                                                                                                                                                                                             >  4:442690/1‑157 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtcg                                                                                      >  1:549688/1‑275 (MQ=255)
                                                                                                                                                                                          tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGGACGCTTTTCTGCGCGAACTGGccgtg                                                                                      >  3:223186/1‑271 (MQ=255)
                                                                                                                                                                                           ggACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                            <  2:442690/157‑1 (MQ=255)
                                                                                                                                                                                               ggCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATTTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGAGGGTGACGCGTGTCTGCGCGAACTGTcggctgt                                                                                    >  3:106372/1‑267 (MQ=255)
                                                                                                                                                                                                 cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAAAGCGGCGAATGAAAGCGCCGGGCATGCGCCGGGTGCCGCCTCGCTGCGCGAACTGGCCTCGTTAATCCTGa                                                                            >  2:254975/1‑278 (MQ=255)
                                                                                                                                                                                                 cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCGTTAAAGCGGTGAATGACAGCGCCGGGCATGCGCCGGGTGAGGCTTTGCTGCGCGAACTGGCGGCGTTAATGcgtg                                                                            >  4:74760/1‑275 (MQ=255)
                                                                                                                                                                                                              tcggttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGAACTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGACTCGGCGTCGGTAATGCTGAGTAtgcgt                                                                    >  3:202547/1‑271 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCTCTTTAAAGCGGTGAATGCCAGCGACGGGCCTGCGGCGGGTCACGCTTTGCTGCCCTCACTTGCGTCTGTACTGCCGAGTTtgcttggtt                                                                >  4:494403/1‑269 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCa                                                             >  2:44094/1‑277 (MQ=255)
                                                                                                                                                                                                                 gttcTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTAACGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTTTGGTGCGttgtag                                                            >  4:205481/1‑274 (MQ=255)
                                                                                                                                                                                                                       gTGATTGAGGACGATACACAAGAACGCAAAATGCCGGGCCAGCTGGGCAACCGAGCCTTACGTGATGCACTACGGACTCTCGCCACCCGCGCCAGTTTTGAGAAAGAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAGATCAGCGACATACCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTc                                                               <  3:561304/269‑1 (MQ=255)
                                                                                                                                                                                                                       gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAAATCAGCGACATGCCCTGGTGTTTACCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCGGAGTATGCTGCGTTGCGTCGACGt                                                      >  4:130328/1‑278 (MQ=255)
                                                                                                                                                                                                                       gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTGTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGGAGGAGGCGTTCCAggg                                                          >  1:167511/1‑272 (MQ=255)
                                                                                                                                                                                                                           ttCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGccc                                                                                                                                                                                <  1:325757/153‑1 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCGGCGACATGCCCTGGTGTTTACCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTCGCTGCGCGTACTGGCTTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTgg                                                 >  2:425503/1‑278 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGGGCGTGACGCTTTTCTGCGCGCACTGGGTTCTTTACTGCTGCGTATTCTTCGTTTCAGCGACTTGc                                                    >  3:322554/1‑275 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCGGCGTTCAAGCGACGTGCTgg                                                 >  2:400533/1‑278 (MQ=255)
                                                                                                                                                                                                                            tCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACAGGCCCTGGTGTTTATACAGCTTCATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTCCTGCGCTAACTGGCGTCGTTCATGCTGATCATGCTGCGTTCCAGCGACGTGc                                                    >  3:207645/1‑275 (MQ=255)
                                                                                                                                                                                                                                gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGaa                                                                                               <  3:78924/228‑1 (MQ=255)
                                                                                                                                                                                                                                gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCCTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGgc                                                <  3:586621/275‑1 (MQ=255)
                                                                                                                                                                                                                                    ttACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACCGCGCCGGGCATGCGGAGGGTGCCGTTTTGCTGCGCGAAATGGCCGCGGTACTGCTGAGAATGCTGGGTGCCAGCCGCGTGCTGGggcggcct                                         >  4:461873/1‑274 (MQ=255)
                                                                                                                                                                                                                                      atcGAATCACGCAAAATGCGGCGACAGCCGAGCGACAGAGCCGACCATGATGCAAGGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAGGCTTTGCTGCGCGAACGGGCGTCGCTAATGCAGAGTATGCGGCGCTCCACGGACGTGGGGGGGCGAcc                                          >  3:388363/3‑274 (MQ=255)
                                                                                                                                                                                                                                         gAATCACGCAAGCTGCTGCGCAGGCAGGACCACACAGCATACCAGGATGCACGTACGCAGCTCACCAACCGCGCCAGTTTGGAAAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAGAGCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGGGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCgg                                       <  3:549688/275‑1 (MQ=255)
                                                                                                                                                                                                                                          aaTCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCTGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTTCGTTCCCGCGACGTGCTTGCGCGACTCGGTggg                                   >  2:133393/1‑278 (MQ=255)
                                                                                                                                                                                                                                            tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGcgtc                                                                                       >  3:186541/1‑224 (MQ=255)
                                                                                                                                                                                                                                             cACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGATAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGa                                 <  2:101957/277‑1 (MQ=255)
                                                                                                                                                                                                                                                gCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTa                                                                                   <  1:186541/224‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  1:322554/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccAGCTGAGCTAAAGCGCCTAACAGGATGCACTGACGCATCTAGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  1:223186/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tagcataaaGCGCCGACAATGATGAACTGACGAATCACGCCAACCGCGCCAGTTTTGAGAACCAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTa              <  3:123838/267‑1 (MQ=255)
                                                                                                                                                                                                                                                                    accTACAGGACCTACCAGTATGCACTGACGAAGCTCGCCAACCGGGCCAGTTTTGAGAAACAACGGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa         <  4:369632/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTACCGCGCCTAGCATGTGTCACGGACGCAGCGCGCCAACCGCGGCAGTTTTGAGGAGCAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGAc        <  4:482492/277‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGa         <  3:108413/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTACTGCTGAGTATGCTGCGTTCCAGCGACGTTCTGGCGCGagc                                          >  3:283882/1‑233 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                 
AGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145040‑2145584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: