BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,937	T→G	K96Q (AAG→CAG)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,937	0	T	G	98.3%	385.1 / NA	115	K96Q (AAG→CAG)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (55/58); total (55/60)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAA  >  NC_000913/4116674‑4117200

aTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCCCCAGACCGGTATTGCTGCGGATATAAGTTTCTAAACCGTCACATTTTAAATGCTCGATGATTTCGTCGGTACGACGGACCTGCCAGACAATGCCGTTGTCGATAGTTTGGTGGGTGTCttttt                                                                                                                                                                                                                                                            >  4:336696/1‑278 (MQ=255)

aTCGGTCACATGGACACGGCCCTGAGCCATTTTCCAGATAAGCCACGTACCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGCTCCATTTCACCTTGGTGCCAGCAAAGTACGGGTCAATCACCAGCCCGGTATTGTTGCGGATATAAGCTTCTAAACTGCCCCGCTTTAAAGCCTCGCATATTTCTTCGGTACTACGCCCCTCCCACACAATGGCGTTTTAGATAAGCTGGAC‑GTTTCtttttt                                                                                                                                                                                                                                                           >  4:261669/1‑277 (MQ=255)

  cgtgaacaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                          <  4:360630/273‑1 (MQ=255)

     tCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCCCGTTTTAAATGCCCGCCAGTTTCTTCGGTACGACGGCCCTGCCAGACACTGCAGTTATAGCAAGGCTGGCCTGTTGCTTTTTcctcg                                                                                                                                                                                                                                                       >  4:407523/1‑275 (MQ=255)

     tCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                          >  1:432826/1‑275 (MQ=255)

     tCACATGGACACGGCCCTGAGTCATTATCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTcc                                                                                                                                                                                                                                                          >  3:221538/1‑275 (MQ=255)

      cacaTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAAATCACCACGACGTGCACGCTCGAGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAAGCTTCTAAACCGTAACGTTTTAAACGCTCGCAGATTTCTCCGGTACGACGGCACTGCCAACCAATGCCGTTATAGAAAGGCTGGCCGGTTTCTTTTTccc                                                                                                                                                                                                                                                         >  1:502867/1‑275 (MQ=255)

              cacGGCCCTGAGTCATTATCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATa                                                                                                                                                                                                                                                 <  1:221538/275‑1 (MQ=255)

               acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGACAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTTTTTTCCCCAGAAAATAg                                                                                                                                                                                                                                                >  1:545243/1‑275 (MQ=255)

               acGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAg                                                                                                                                                                                                                                                <  1:534237/275‑1 (MQ=255)

                        aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTTTTTTTCCCAACCAATAGTGttttacggct                                                                                                                                                                                                                                    >  4:111674/1‑272 (MQ=255)

                         gTCATTTTCCAGATAAGCCACGAATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACg                                                                                                                                                                                                                                      <  3:432826/275‑1 (MQ=255)

                              tttCCAGATAAGCCACCTATCAACCCTACCCAACAGCCATGCACCCCGCCGTGCACGCTGGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGt                                                                                                                                                                                                                                 <  3:96320/275‑1 (MQ=255)

                                  cAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCATTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATCAGCTGGCCGGTTTCTTTTTCCCAGACCATACTGGTTTTACTCTGTTTCGTaa                                                                                                                                                                                                                           >  4:268174/1‑277 (MQ=255)

                                    gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTaa                                                                                                                                                                                                                           >  1:27491/1‑275 (MQ=255)

                                    gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTaa                                                                                                                                                                                                                           >  1:29927/1‑275 (MQ=255)

                                       aaGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTAACGTTTTAAATGCTCGCAGATTTCTGCGGTACGCCGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTctagatatcca                                                                                                                                                                                                                     >  4:209478/1‑268 (MQ=255)

                                            aCGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATCGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATCGTGGTTTCACCCTTGTCGGTAATACCGTTAgga                                                                                                                                                                                                                >  4:63120/1‑276 (MQ=255)

                                                 ccAACCGCACCAAACAGCAATTCACCCCGACGTGCACGCTCGCGAGAGCTTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAt                                                                                                                                                                                                           <  4:352249/277‑1 (MQ=255)

                                                   aaCCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATTCCGATTGCTGCAAttt                                                                                                                                                                                                         >  2:261379/1‑278 (MQ=255)

                                                        tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCg                                                                                                                                                                                                    >  2:471501/1‑278 (MQ=255)

                                                         aCCCAACAGCAATTCACCACGACCGGCACGCTCGCGGGGGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAt                                                                                                                                                                                                      <  3:27491/275‑1 (MQ=255)

                                                                    aTTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGAGGGTACTGCCAGACAATGGCGTTATAGAACGGCTGGCCGGTGTCTTTTTCTCAGACAAGAGTGGTTTGACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACGGctattg                                                                                                                                                                                        >  4:396426/1‑276 (MQ=255)

                                                                       acctcactcgTGCACGGCCGCGTGAGCCTTCCATATGGCCGAGTACCCACCTCACTTTGGTTCCAGAAAAGGACGGGTCAATCACCAGACCGGTATTGCTGCTGATATCATCTGCTGAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCt                                                                                                                                                                                     <  4:463320/270‑1 (MQ=255)

                                                                          cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAg                                                                                                                                                                                                                                                <  4:116620/216‑1 (MQ=255)

                                                                          cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAg                                                                                                                                                                                                                                                >  2:116620/1‑216 (MQ=255)

                                                                              acgTGCACGCTCGCGAGAGCATTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                              <  2:268174/278‑1 (MQ=255)

                                                                              acgTGCAAGCGCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                              <  2:63120/278‑1 (MQ=255)

                                                                                gTGCACGCGCGCGAGAGACTTCCACATGGTAGAGTATCCACGTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACGGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCa                                                                                                                                                                                                                                                        <  4:502398/202‑1 (MQ=255)

                                                                                 tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                              <  3:573749/275‑1 (MQ=255)

                                                                                 atattccgcgcgAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                              <  3:206204/267‑1 (MQ=255)

                                                                                  gCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTGTTAAATGCTCGCAGATCTCTGCGTTACGACGGCACTGCCAGCCAATGGCGTTATTGATAGGCTGGCCGGTTTCTTTTCTTCAACCAAGTGTGGTTTCACGCTGGTTCGCAATACCGATATCTGGCATATTAACGGAACGGATACCGGCTTTccgc                                                                                                                                                                               >  3:62079/1‑270 (MQ=255)

                                                                                    aCGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGAAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTGTTTTTTTCCCAGACAATAGTGGTTTCCAGCTGGATCGTAATACCGTTAGCTGCAATTTGATCGGACCTGATATTGGCTTTCGTCAGctgtt                                                                                                                                                                        >  2:37184/1‑274 (MQ=255)

                                                                                           cgAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGTCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGTTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                                                                    >  1:529307/1‑275 (MQ=255)

                                                                                                   ttCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGTTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                             >  1:453318/1‑274 (MQ=255)

                                                                                                                   aTCCACTTCACTGTGGTGCCAGAAAAGTACGGGTCAATCACCAGCACGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                         <  2:250774/278‑1 (MQ=255)

                                                                                                                    tCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTCGATCGGACCTGATATCGGCTTTCGCTAGCACTTCTCCCAGCGTGTAGCTTTGTGTTGCCGAgtat                                                                                                                                        >  4:527611/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGTCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCCGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCTCAGATTTcc                                                                                                                                     >  2:365346/1‑278 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCTGCTTTCGCCAGCACTTCTACCAGCGTGGTGCTTTCGGTGGCCCAGAtt                                                                                                                                        >  1:155211/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                                                                                                        >  1:179248/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCACCCCTTCTACCAGCTTGGGGCTTTGGGTGGCCCGGAtt                                                                                                                                        >  1:168784/1‑275 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTAGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTGTTTCCCAGACAATAGTGGTTTAACCGTGGTTCGTAATACCGATCGCTGCAATTTGTACGGAAAGTATATGGGGTTTCACCCGACCTTCTAGCAGCGGTGGGCTTTtggtggtcaagtgtttg                                                                                                                                     >  4:296286/1‑267 (MQ=255)

                                                                                                                       acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGGTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATCGTGGTTTCACGCTGGTTCGTAATCCCGTTAGCTGCACTTTGATCGGTACTGATATCGGATTTCGCCAGCATTTCTACCAGCGTGGACCTTTGGCTGGCCCCGAt                                                                                                                                         >  3:263773/1‑274 (MQ=255)

                                                                                                                          tcacatTGGTGCCAGAAAAGTACGGGTCAAGCCCCAGACCGTTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTTCGGGACGCCGGCACTGCCAGACAATGGCGTTATTGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                  <  4:323772/278‑1 (MQ=255)

                                                                                                                              tttGGTGCCAGAAAAGTACGGGTCAATATCCAGACCGGTATTGCTGCGGATATAATCTTCTGAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                  <  3:100475/274‑1 (MQ=255)

                                                                                                                               ttgggtccAGAAAAGTACCGGTCCACAACCAGACCGGTATTGCTTCGGATATAATCTTCTAAACCGTCACGTTTTAAAGGCTCGCAGATTTTTGCGGTAAGACGGCACTGCCAGACAATGGCGGTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                             <  4:37184/272‑1 (MQ=255)

                                                                                                                               ttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                             <  2:499234/278‑1 (MQ=255)

                                                                                                                                 ggTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGa                                                                                                                                                                                                       >  1:11017/1‑202 (MQ=255)

                                                                                                                                  ttgacaaaaaaGTACAGGGCAATGACCCTACCGGTGTTGCTGCGGATATAATCTTCTAAACCGTTGCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACCATGGCGTTATAGATAGGCTGGCCGGTGTCTTTTTCCCAGACAAGAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                             <  3:502867/268‑1 (MQ=255)

                                                                                                                                   tGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCCTGCTGGTTCGTAATa                                                                                                                                                                                                                         >  1:234853/1‑182 (MQ=255)

                                                                                                                                     ccAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCTTGGAGCTTTGGGTGGCCCGGATTGCCATTGGGTCGTGTTc                                                                                                                       >  2:503362/1‑278 (MQ=255)

                                                                                                                                       aGAAAAGTACGGGTCAATCAACAGACGGGTATTGCGGCGGATATAATCTTCGAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGGACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGa                                                                                                                                                                                                                     <  3:234853/182‑1 (MQ=255)

                                                                                                                                        gAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCCCCAGCCCTTCTCCCATCGTGGCTCTTTTGGTGTCCCCGATTTCCCTGGGGTCGTGtttttc                                                                                                                    >  4:395014/1‑274 (MQ=255)

                                                                                                                                                  ggtaatacACCAGACCGTTATTGCTGCGGATATAATCTTCTAAGCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAAcc                                                                                                             <  3:369092/267‑1 (MQ=255)

                                                                                                                                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACTAGTGTTGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCTTGTTCTA‑CCCCACCTGGtt                                                                                                        >  2:510044/1‑278 (MQ=255)

                                                                                                                                                   gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCGGATTTCCTTTGGGTCGTGTTCTA‑CCCAACATg                                                                                                           >  3:36683/1‑275 (MQ=255)

                                                                                                                                                                   gTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTTGATTTg                                                                                           >  2:181557/1‑275 (MQ=255)

                                                                                                                                                                              gATTTAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACACTGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGTCCTGCGCCAGCACTTCTCCCACCGTTGCGCTTTGGTTGGCCCAGATTTCCAGTGGGCCGTGTGCTA‑CCCACACTGGTTTTGGGTAGCTGTGCTTAAATTCGCGCt                                                                             >  4:153669/1‑278 (MQ=255)

                                                                                                                                                                              gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc                                                                                >  1:50687/1‑275 (MQ=255)

                                                                                                                                                                                  taATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  2:236194/278‑1 (MQ=255)

                                                                                                                                                                                  taATCTTCGAAACCGTCACGTTTGAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  2:527611/278‑1 (MQ=255)

                                                                                                                                                                                   aaTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACTAGCGTGGAGCTTTGGGTGGCCCAGAGTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTGGCTCAAATTCGCGCTGc                                                                           >  1:533397/1‑275 (MQ=255)

                                                                                                                                                                                    atctgccataCCGTCAAGTTTTAAAGGCTGGCAGATTTCTGCGGTACGACAGCAGTGCCATACAATGGCGTTATAGGTATGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCTAGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATAGCGGCGTTCGCCAGCACTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCGAACCTGGTTTTGTGTAGATTTGCTCAAATTCGCGCTGCg                                                                          <  3:352549/266‑1 (MQ=255)

                                                                                                                                                                                     tctgctAAACCGTCACGTTTTAAATTCTCGCAGATTTCTGGGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTTGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                         <  3:452007/275‑1 (MQ=255)

                                                                                                                                                                                        tctAAACCGTCACGTTTTAAATGTTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                       <  1:512305/274‑1 (MQ=255)

                                                                                                                                                                                         ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAAATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                     >  1:491054/1‑275 (MQ=255)

                                                                                                                                                                                          tAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                  <  2:396426/277‑1 (MQ=255)

                                                                                                                                                                                          tAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                  <  2:279931/277‑1 (MQ=255)

                                                                                                                                                                                           aaaCCGTCACGTGTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACAAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                                <  2:136907/278‑1 (MQ=255)

                                                                                                                                                                                            aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTGTGGGTAGATTGTCGCAAATTtgcgtgtcgagcggcaa                                                                  >  3:470949/1‑262 (MQ=255)

                                                                                                                                                                                                ttCACGTTTTGAATGCTCGCGGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                           <  2:383593/277‑1 (MQ=255)

                                                                                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTGCAACCAGTGTGGAGCTGTGGGTGGTCCAAGTGTCGCTTGGGGCGTGTTCTA‑CACAGCTGGGTTGCGGGGAGAGTGGTTGACATTCGAATTGCGAGACGCTAGTGgtaag                                                          >  4:477328/1‑276 (MQ=255)

                                                                                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGa                                                              >  1:114839/1‑274 (MQ=255)

                                                                                                                                                                                                 tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGCTTTGGGTAGGTTTGCACAAATTCGCGCTGCTACATGCTAATCATAtt                                                          >  2:165731/1‑278 (MQ=255)

                                                                                                                                                                                                      ttttAAATGTCCGCAGATTTCTGCGGTACGAGGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                        <  3:416349/275‑1 (MQ=255)

                                                                                                                                                                                                        ttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCTAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCg                                                   <  2:111674/278‑1 (MQ=255)

                                                                                                                                                                                                        ttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                                                      <  1:104705/275‑1 (MQ=255)

                                                                                                                                                                                                           aaTGTTTGCAGATTTTTGGGGTACGACGGCACTGCCAGAAAATGGCGTTGTAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGTTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  4:471501/278‑1 (MQ=255)

                                                                                                                                                                                                           aaTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  2:173052/278‑1 (MQ=255)

                                                                                                                                                                                                            aTGCTCGCAGATTGCTGTGGGCCGAAGGCACTTCCCTACAATGGAGTTATTGGTAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  4:212800/278‑1 (MQ=255)

                                                                                                                                                                                                              tctctCAGCTTTCTGCGGTCCGGCGGCACTGCCAGAACATGGCGTTTTAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTGCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  3:453318/274‑1 (MQ=255)

                                                                                                                                                                                                              gtgtgCAGATTTCTGTGGTACGACTGCCCCGCGAGACAAATGCGTTGTAGCTAGGCTGGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCGGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  3:29927/271‑1 (MQ=255)

                                                                                                                                                                                                              gCTCGCAGTTTTCTGCGGTACCACGGCACTGCCAGACAATTGCTTTATCAATAGGCTGGCCGGTTTCTTTTTCCCAGACAATTGTGGTTTCCCGCTGGTTCGTAATACCGATATCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  3:533397/275‑1 (MQ=255)

                                                                                                                                                                                                              gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCTGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  1:284243/275‑1 (MQ=255)

                                                                                                                                                                                                              gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  1:470949/275‑1 (MQ=255)

                                                                                                                                                                                                                tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGGCCTGCGACCCGCTTATGATATTGGcctggccct                                               >  3:182799/1‑266 (MQ=255)

                                                                                                                                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAtt                                                                                                                                                                                                          >  1:256844/1‑119 (MQ=255)

                                                                                                                                                                                                                 cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACTCGCCAATGTTTTTGGCATCGTGagc                                              >  3:321844/1‑272 (MQ=255)

                                                                                                                                                                                                                    aGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                  <  1:182799/251‑1 (MQ=255)

                                                                                                                                                                                                                     gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAt                                                                                                                                                                                                      <  3:256844/119‑1 (MQ=255)

                                                                                                                                                                                                                     gATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTAGAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                <  1:321844/268‑1 (MQ=255)

                                                                                                                                                                                                                                tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTTGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcc                               >  1:479006/1‑273 (MQ=255)

                                                                                                                                                                                                                                  cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                          <  2:395014/277‑1 (MQ=255)

                                                                                                                                                                                                                                       gCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                    <  2:209478/278‑1 (MQ=255)

                                                                                                                                                                                                                                         ccTGCCAGATAACGGCGTTGTTGTAAGGTTGGCCGGTTTCTTTTGCCCAGACAATAGTGGGTTCACGCTGGTTCGTAATACAGATAGCCGCAATTTGGACGGACCTGATATCGGCTTTCGCCAACACTTCTACCCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGGCtggtg                     <  3:275137/274‑1 (MQ=255)

                                                                                                                                                                                                                                         aCTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                     <  1:346203/275‑1 (MQ=255)

                                                                                                                                                                                                                                                     tGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATCATATTGGCAt                                                     >  2:3707/1‑231 (MQ=255)

                                                                                                                                                                                                                                                      ggCGTTATCGTTGGGCTGGTCGGTTTCTTTGTCCCATGCAATATTGGTTGTCCGCTGGGTCTGATTACGGATAGCGGCAATTGTGTCGTAACTGGTTGCGGCTTTCGCCGGCACGTCTGCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAGCCTGGTTTTGGGTAGNTTTGCTCGAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGCTCCATTACGACCGCGCGGGAGATGGTGGTGCCCTGGTCGAgcg     <  4:280139/278‑1 (MQ=255)

                                                                                                                                                                                                                                                       gtgTTAGAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcgc    <  2:336696/276‑1 (MQ=255)

                                                                                                                                                                                                                                                            atagatagGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCAGAACTGAGATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCaa  <  1:62079/275‑1 (MQ=255)

ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAA  >  NC_000913/4116674‑4117200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`