Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,138,892 G→A V48V (GTC→GTT gldA ← glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,138,8920GA98.4% 389.4 / NA 124V48V (GTC→GTTgldAglycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (57/64);  minor base C (2/0);  total (59/65)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

GGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATT  >  NC_000913/4138621‑4139164
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                
ggTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACa                                                                                                                                                                                                                                                                               >  3:171962/1‑275 (MQ=255)
  tCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTTCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGAAGGTAGATCCCATTTTGCGAACATTCCCCCCCAAACGGCGCAATTTCTACTACCAGTCCATCACATTTACAACTTTTCTCCACAGTga                                                                                                                                                                                                                                                                           >  4:141585/1‑276 (MQ=255)
  tCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAg                                                                                                                                                                                                                                                                              >  1:284273/1‑274 (MQ=255)
    aaaCTCACCCGAATCGGTGTAGATAATAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACa                                                                                                                                                                                                                                                                               <  3:48467/271‑1 (MQ=255)
         cACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTAGCGAGGGTTTTTCCGCCACCGATACCGAGAATTTCGCCAGAATGCGCAGTCTCCGCGATGCCACGAAGACGGTAGATCTCAATTTGCGAACAGTCCCCGCCAAAAGGCGCAATTCCGACTACCAATCCACCATCTTTAAAGCTTTTCTCAACAGTGGagcgaaca                                                                                                                                                                                                                                                                   >  4:40611/1‑271 (MQ=255)
         cACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCGACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGGTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATTTCATGTTTCGAACATTCACCGCCAAACGCCGCACTTTACACCAACAGACCAGCATCTGTAAACTTTTTCCCAACCGTGGATTGa                                                                                                                                                                                                                                                                      >  3:456135/1‑275 (MQ=255)
            ccTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCaaaa                                                                                                                                                                                                                                                                >  2:174122/1‑278 (MQ=255)
                      gtAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACaa                                                                                                                                                                                                                                                      >  4:162548/1‑278 (MQ=255)
                       tAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAAc                                                                                                                                                                                                                                                        <  3:284273/275‑1 (MQ=255)
                             aCAGACAAGGCGCTCGACGGTGCGTCGGTAGGGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTg                                                                                                                                                                                                                                                 <  2:69114/276‑1 (MQ=255)
                              cAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATGTCTACTACCAGTACACCATCTTTAACGCTTTTCACAACAGTGGATTGAGAAACACCTAAAACAAATTTgcc                                                                                                                                                                                                                                               >  4:376566/1‑275 (MQ=255)
                              cAGAAAATGCGCTGCACGGTGCATCGGGAGAGGCGTTAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTg                                                                                                                                                                                                                                                 <  4:482432/275‑1 (MQ=255)
                               aGACAATGCGCTGCACGGTGCATCGGTCGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTAACGAGGGTTTTTCCGCCCCCGATACCGAGAATTGTGCCCCACTGCGCAGTCGTCGCGAGGCCACGCAGACGGTCGCACCCATCTTGCGCACCTTCACTGCCAAACGGGCCAATTTCTACTATCACTCCACCATCTTTAAATCCTCTCCCAACAGCGGATTGACCAAAACCCAAAACAAAttct                                                                                                                                                                                                                                                 >  3:606797/1‑272 (MQ=255)
                               aGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCCCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTg                                                                                                                                                                                                                                                 >  1:204840/1‑274 (MQ=255)
                                    aTGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTcacccacc                                                                                                                                                                                                                                        >  2:45877/1‑278 (MQ=255)
                                    aTGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTcacc                                                                                                                                                                                                                                            <  1:320723/274‑1 (MQ=255)
                                    aTGCGCTGCACAGTGCATCGATAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAACTTGTcacc                                                                                                                                                                                                                                            <  1:456135/274‑1 (MQ=255)
                                                 tGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACCTTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACCGTGGATTGAGCAAAACCTAAAACAAATTTGTCCCCCACCACTCACCaacct                                                                                                                                                                                                                            >  4:183962/1‑273 (MQ=255)
                                                 tGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCa                                                                                                                                                                                                                                >  3:407502/1‑273 (MQ=255)
                                                      cGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGCTCTCATTTTGCGAAAATTCACCGCCAAACGGCCCAATTTCTACTACCAGTCCAGCATCTTTAAAGATTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCt                                                                                                                                                                                                                         >  1:181344/1‑275 (MQ=255)
                                                      cGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCGCATTTTGCGAACATACACCGCCAAAAGGAGAAAGTGCTACTACTAGTCCAGCATATTTAAAGCTTTTCACAAAAGTGTAATGAGCAAAACATAAAACAAATTGGGAACCCCCAACTaaaccacacgctgca                                                                                                                                                                                                                      >  4:255579/1‑265 (MQ=255)
                                                      cGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCCCCGCGATGCCACGCAGACGGTCGTTCCCATTTTGCGAACATCCACCGCCAAACGGCGCAAGTCCTACTACCAGTCCAGCATCTTTAAAGTTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGca                                                                                                                                                                                                                      >  2:238290/1‑277 (MQ=255)
                                                      cGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGACACAATGCGCAGTCTCCGCGATGCCACGCAGACGGGCGATCTCATTTTTCGAACAATCACCGCCAACCGGCCCAATTTCTACTACCAATCCAGCATCTTAAAAGCTTTTCCTCACAGTGGGTTTATGAAAACCTAAAACAAATTTGTCACCCAACACTAACAAGGGTTcc                                                                                                                                                                                                                         >  3:608901/1‑274 (MQ=255)
                                                           gagGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTTCGCCACACTGCGCAGTCTCCGCGATGCAACGCAGACGGTCGATCCCATTTTGCGAACATTCACCGCCAAACGGCGCAATTCCTACTACCACTCCAGCATCTTTAAAGCATTTTTCAACAGTGGATTGAGCACAACACAAAACAAACTTGTCCCCCATCACCAAATAGCGtttttcgag                                                                                                                                                                                                                    >  3:552238/1‑268 (MQ=255)
                                                           gagGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGAGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCATCCACCACTAACCAGCGTTCTGCTAGCg                                                                                                                                                                                                                  >  2:91096/1‑277 (MQ=255)
                                                                 aTAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGCTTGAGCAACACCTAAAACACATTTTTCACCCACCACTAACCACCGTTCTGCCAGCGCCTTcc                                                                                                                                                                                                            >  2:410376/1‑276 (MQ=255)
                                                                  tAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTc                                                                                                                                                                                                             <  1:244705/275‑1 (MQ=255)
                                                                        gTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACTCTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTTAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAACGTTCTGCCAGCGGCTTCAGGTAt                                                                                                                                                                                                       >  1:522978/1‑275 (MQ=255)
                                                                             aTCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCa                                                                                                                                                                                                <  2:166741/277‑1 (MQ=255)
                                                                                   aCCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCCCCGCGATGCCACGCAGACGGTCGATCTCCTTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCCTCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAATCAGTGTTCTGCCAGCGGACTCAGGTATTCGccaaagc                                                                                                                                                                                             >  1:1623/1‑269 (MQ=255)
                                                                                       gAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAAt                                                                                                                                                                                      <  2:518785/277‑1 (MQ=255)
                                                                                          cacCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATc                                                                                                                                                                                     >  3:67155/1‑275 (MQ=255)
                                                                                              cATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCCCCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAg                                                                                                                                                                              <  2:139702/278‑1 (MQ=255)
                                                                                                gtaattgtgCCAGTGCTTTTGCACTAGCGAGGGTTCTTCCGCCACCGCTACCGGGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACAGTCGATCTCATTTTGCGAACATTCACCGCCGAACGGCGCAATTTCTACTACTAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCACCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCa                                                                                                                                                                               <  3:181344/270‑1 (MQ=255)
                                                                                                    aTGTGCCAATGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGACACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCGGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAgcgc                                                                                                                                                                           <  1:506757/275‑1 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCTGGGGGTTTTCTGACAACGATACCGTGAATTGCGCCACACTGCGCAGTGTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAGCCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGa                                                                                                                                                                     <  2:255772/278‑1 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATTCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCCAAACCTACAACAACTTTGTCACCCACCCCTAACCCGCCTCCTGCCACCAGCTTCATGTATTCGCCCTAACCATCAATCCCATccaccacctgga                                                                                                                                                                     >  4:101534/1‑267 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCCCCGCCAAACGGCGCAATGTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCACCCACTAACCAGCGTTCTCCCAGCGGCTCCAGGTATTCTCCCAAACGATTAATCCCAACAGCGcccaca                                                                                                                                                                      >  4:216787/1‑274 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACCTCAGCCCCCtcg                                                                                                                                                                      >  2:385104/1‑275 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCCCATTTTGCGAACATTCACGGCCATACGGCGCAACTTCTACTCCCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGAAACACCTACAACAAATTTGTCCCCCACCACTAAACACCGTTCTGCCAGCGGCCTCACGTATTAGCCCAAACGATAAAacacttaacgcccct                                                                                                                                                                        >  4:427102/1‑264 (MQ=255)
                                                                                                       tgCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCCCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTCCCAGAGGCTTTAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCt                                                                                                                                                                        >  1:354214/1‑275 (MQ=255)
                                                                                                        gCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAGCAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGAt                                                                                                                                                                    <  2:125886/278‑1 (MQ=255)
                                                                                                         ccAGTGCTTTGTCAGTATCCAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTgg                                                                                                                                                                      <  1:120815/275‑1 (MQ=255)
                                                                                                         ccAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTgg                                                                                                                                                                      <  1:374170/275‑1 (MQ=255)
                                                                                                                       gTATCGAGGGTCTTTCCGACACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAAAGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATGTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGa                                                                                                                                                     <  2:229302/278‑1 (MQ=255)
                                                                                                                            gAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGa                                                                                                                                                     <  1:576842/273‑1 (MQ=255)
                                                                                                                              gggTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACCAATTTGTTACTCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCTCAGACGATGAATCACATCAGCGCCCTGGATGGATTTACCCGGTGATTGa                                                                                                                                                 >  1:285801/1‑275 (MQ=255)
                                                                                                                                   tttCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCCCCCAGCCGATTAATCCCATCAGCCCCCTGGATTTATTCCCCCGGTGATTTAAACATGCg                                                                                                                                         >  4:61683/1‑278 (MQ=255)
                                                                                                                                    ttCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCg                                                                                                                                         <  2:43057/277‑1 (MQ=255)
                                                                                                                                     tCCGCCACAGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACCGTCGTTCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAAGCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATg                                                                                                                                           <  1:83906/274‑1 (MQ=255)
                                                                                                                                       cGCCACCGCTACCGAGAATTGCGCCCAACTGCGCAGTCGCCGCGATGCCACGCAGACGGTCGATCTCATTTTTCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATg                                                                                                                                           <  3:204840/272‑1 (MQ=255)
                                                                                                                                       cGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATg                                                                                                                                           <  3:588009/272‑1 (MQ=255)
                                                                                                                                            ccGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGGTCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAAt                                                                                                                                <  2:318206/278‑1 (MQ=255)
                                                                                                                                                 aCCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTg                                                                                                                              <  1:441553/275‑1 (MQ=255)
                                                                                                                                                       aaTTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCtt                                                                                                                        <  1:67155/275‑1 (MQ=255)
                                                                                                                                                         ttgccgcacaCTTCCCACTCCCCGGAATGCCCCGCAGACGCTCGATCTCATTTTTCGAACATTCACCGCCAAACGGCACCATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAGAACAAATTTGTCCCCCACCACTAACCAGCATTCTGCCAGCGGCTTNAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTagag                                                                                                                   <  4:288161/272‑1 (MQ=255)
                                                                                                                                                           gcgcCACACTGCGCAGTCTCCGCGATGCCACGCAGATGGTCGATCTCAATTTGCGAACAGTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGAt                                                                                                                 <  4:467467/278‑1 (MQ=255)
                                                                                                                                                            cgcCACACTGCGCAGTCTCCGCGATGCCACACAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATg                                                                                                                >  2:135769/1‑278 (MQ=255)
                                                                                                                                                                    tGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTACAACAAATTTGTCCCCCACCACTAACCAGCGTTCTTCCATCGGCTTCAGGTATTCGCCCAGACGATTAATCACCTCAGCGCCCTGGCTGTATTTACCCGGTGATTGAATCATGCGGTCCATCATTGCTCCTTTCTCTATGAGTAGTGc                                                                                                        >  4:394416/1‑278 (MQ=255)
                                                                                                                                                                      cgcAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCNTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACACGGTGATTGAATAATGCGGTCCATAATTTCTCCTTTAGAGATGAGTCGTTCCa                                                                                                      >  4:293874/1‑278 (MQ=255)
                                                                                                                                                                       gcAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCa                                                                                                                                                                                                <  4:159273/187‑1 (MQ=255)
                                                                                                                                                                           tctcCGCGATGCCACGCAGACGGTCGATCGCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCCCAACAGTGGATTGAGCAAAACCTCAACCAAATTTGTCATCAACCACTAACTACCGTTCTGCCAGCGGCTTCAGGTACTCGCCCAGCCGCTTAATCACTGCCGCGCCCTGGACGTATTTACCCGGCTATTGAAGAATGAGGTCCATAATCGCTCCTTTAGATAGGtgtagttcacc                                                                                                     >  3:300495/1‑269 (MQ=255)
                                                                                                                                                                            ctcCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCg                                                                                                                                                         >  3:587067/1‑221 (MQ=255)
                                                                                                                                                                             tcCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGACTAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATg                                                                                                  >  1:118832/1‑275 (MQ=255)
                                                                                                                                                                             tcCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCgg                                                                                               <  2:216787/278‑1 (MQ=255)
                                                                                                                                                                             gccGCGATTCCACGCAAACGGTCGATCGAACTTTGCGAACATTCACCGCCAAACGTCGCTATTTCTACTACCATTCCAGCATCTTTAAAGCTTTTTTCAACAGTGGATTGAGCAAAACCTAACACAAATTTGTCACCCGCCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCgg                                                                                               <  4:157914/277‑1 (MQ=255)
                                                                                                                                                                             cacgcgATGCCACGCAGACGGTCGATCTCATTTTGCGAACAATCACCGCCAAACGGCTCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTGTCTCAACAGTTGATTGAGCAAAACCTAAAACAAATGTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCgg                                                                                               <  2:95551/276‑1 (MQ=255)
                                                                                                                                                                                gcgATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGa                                                                                                                                                     <  1:587067/221‑1 (MQ=255)
                                                                                                                                                                                     gCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCAtt                                                                                           >  1:154459/1‑274 (MQ=255)
                                                                                                                                                                                       cacacaGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGt                                                                                      <  4:135769/277‑1 (MQ=255)
                                                                                                                                                                                             gACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGtt                                                                                <  2:439331/277‑1 (MQ=255)
                                                                                                                                                                                               cGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCg                                                                              <  2:289396/277‑1 (MQ=255)
                                                                                                                                                                                                ggTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGaa                                                                            <  2:293874/278‑1 (MQ=255)
                                                                                                                                                                                                   cGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACtt                                                                         <  2:141585/278‑1 (MQ=255)
                                                                                                                                                                                                   cGATATCATTTTGCCAACATTCACCGCCAAACGGCGCAATTACTACTACCAGTCCCGAAACTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGaa                                                                            <  1:293718/275‑1 (MQ=255)
                                                                                                                                                                                                     aactcAATTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACCGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAAc                                                                           <  3:289742/272‑1 (MQ=255)
                                                                                                                                                                                                       ctcATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGAGTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTtctc                                                                      <  2:437263/277‑1 (MQ=255)
                                                                                                                                                                                                        ccATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTAATACCAGTCCAGCATCTTTAAAGCTTTTCTCACCAGTGTATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGTATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTACAGTCGTGTTCGAACTtct                                                                       <  1:62910/274‑1 (MQ=255)
                                                                                                                                                                                                            tttGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCCCCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCCGCGCCCTGGCTGTATTTACCGGGTGATTGAATAATGCGGTCCATAATTCCCTCTTTAAAAATGAGTTGTCCCAAATGCGGACTTCCTGTCGTTTTCGACTTtcttaa                                                                    >  3:174553/1‑271 (MQ=255)
                                                                                                                                                                                                               tgatccATTCACCGCCAAAGCGCGCAAGTGCTACGACCGGTCCTGCATGTTTAAAGGTTTACTCAACACTGGATTGAGCAACACCTAAAACAAATTGGTCACCCACCTCTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGGACTTCTCTATAGCTGa                                                             <  4:91096/273‑1 (MQ=255)
                                                                                                                                                                                                                 caaCATTACCCGCCACACGGCGCAATTTCTACTACCAGTCCAGCCTCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCGAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCt                                                               <  1:552238/273‑1 (MQ=255)
                                                                                                                                                                                                                          ccGCCAAACGGCGCAATTTCTACTACCCGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCg                                                     <  1:608901/275‑1 (MQ=255)
                                                                                                                                                                                                                               aaacatagaaaTTTCAACTACCCGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGAATGAGCAAAACCTAAAACAAATTTGTCACCCCCCACTAACCAGCGTTCGGCCAGCGGCTTCAGGTATTCGACCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGgtg                                             <  4:226375/269‑1 (MQ=255)
                                                                                                                                                                                                                                  cGGCGCAATTTCTACTACCATGCCAGCATCTTTAAAGCGTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCTCCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTg                                          <  2:85007/278‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcAATTTCTCCTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATAGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTCTTTACCCGGTGATTGCATAATTCCGTCCCTAATTGCCCCTTTAGAGATGAGTAGTGCGACACGCTGCATTCTCATCGTTCTCGAACTTATCTACAGTTGCCACAACCGCAGGGgcgtggcgc                                       >  4:19089/1‑273 (MQ=255)
                                                                                                                                                                                                                                           ttCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATttgt                                 <  2:162548/278‑1 (MQ=255)
                                                                                                                                                                                                                                                 cacCAGTCCAGCATCTTTAAAGCTTTTCTCAACCGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCACTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCt                           <  2:419770/277‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccattccattATCTTTAAATCCTTTCTAATCGAGGGATTGAGCAAACCCTAATACAGATTCGTCATCCACCCCTAACCAGCGTGCAGCCAGCGGCGTCAGGTATTGGCGCAGGCGATTAAAGCCATCAACGGCGTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAATAGTGCCAAATGCGGCATTCCAGTCGTGTTAGAACTTCTCTATAGCTGACTCTACCGCAGGAGTATTGAGCATGTGTTgcg                             <  3:518233/274‑1 (MQ=255)
                                                                                                                                                                                                                                                    cAGTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCt                           >  1:155894/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      gTCCAGCATCTTTCAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCCTTCCCGTCGTGTTCGAACTTCTCTATAGCGGACTCTACCGCCGGGGTTTTGAGCATTTGTTGCGCTAc                         >  1:97820/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      gTCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCGAATGCGGCATTCCAGTCGTGTTCGAACCTCTCTTTAGCCGACTCTACCGTAGGGGCTTTGACCATTTGTTGCGATAc                         >  3:490621/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       tCCAGCATCTTTAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCt                     >  2:23290/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                 ttAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCTGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCa              <  1:222370/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ttAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTTCATCTTAGGGCCgg            >  2:333079/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                 ttAAAGCTTTTCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGc               >  1:584231/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                        ttttCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGTTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGAt       <  2:376566/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tttCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATATTGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACCCTACCGCAGGGGTGTTGAGCATTTGTTTCGCTACATCTAAGGGCCGTGTGAt       >  1:304386/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                         tttCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTCCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCCCTATAGCTGACTCTCCCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGAt       >  3:230152/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                          ttCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCATACGATTAATCACATCAGCGCCCTGGCTGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGATATGATTCGTTCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTCTAGCTGACTCTACCGCCGGGCTCGTGGTTCTTTGTTGCTATACACCCATGGGCAGGGTGATGGAtg  >  4:305268/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                          ttCTCAACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATgg     >  1:423726/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                
GGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGTGCTTTGGCAGTATCGAGGGTTTTTCCGCCACCGATACCGAGAATTGCGCCACACTGCGCAGTCTCCGCGATGCCACGCAGACGGTCGATCTCATTTTGCGAACATTCACCGCCAAACGGCGCAATTTCTACTACCAGTCCAGCATCTTTAAAGCTTTTCTCGACAGTGGATTGAGCAAAACCTAAAACAAATTTGTCACCCACCACTAACCAGCGTTCTGCCAGCGGCTTCAGGTATTCGCCCAGACGATTAATCACATCAGCGCCCTGGATGTATTTACCCGGTGATTGAATAATGCGGTCCATAATTGCTCCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATT  >  NC_000913/4138621‑4139164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: