Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,907,316	T→C	6.2%	E209G (GAA→GGA)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,316	0	T	C	6.2%	202.0 / 2.6	64	E209G (GAA→GGA)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base T (25/35);  new base C (2/2);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GTATTTACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTT  >  NC_000913/2907168‑2907458                                                                                                                                                     |                                                                                                                                               gTATTTACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCtt                                                                                                                                                >  1:335426/1‑149 (MQ=255)  tATTTACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGACAAAGTGATGTCTTTGGCCAGTTCATAACCATCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTc                                                                                                                                               <  1:137985/149‑1 (MQ=255)    tttACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGt                                                                                                                                             >  2:527308/1‑149 (MQ=255)     ttACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTc                                                                                                                                            >  3:233472/1‑149 (MQ=255)       aCCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCAc                                                                                                                                          >  1:351882/1‑149 (MQ=255)        ccATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCAcc                                                                                                                                         >  1:158688/1‑149 (MQ=255)                   gAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAgtgt                                                                                                                              <  4:120049/149‑1 (MQ=255)                    aaTTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGtt                                                                                                                             >  4:19987/1‑149 (MQ=255)                     agtCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTc                                                                                                                            <  4:487361/147‑1 (MQ=255)                     aTTCAGCAGCTGCGCAGTCTATCGCCAAAGTGATGTCTTTGACGAGTTCATAACCAGCAGCTTTAACAGCTTCAGCTATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTCCGTCACCAACAGCAGTGTTc                                                                                                                            >  4:102707/1‑149 (MQ=255)                     aTTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTCCGTCACCAACAGCAGTGTTc                                                                                                                            >  4:500005/1‑149 (MQ=255)                      ttCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCa                                                                                                                           >  3:230561/1‑149 (MQ=255)                       tCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCAt                                                                                                                          <  3:102052/149‑1 (MQ=255)                       tCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCAt                                                                                                                          <  2:256877/149‑1 (MQ=255)                        cAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATg                                                                                                                         >  4:381920/1‑149 (MQ=255)                            aGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCtt                                                                                                                     <  1:493418/149‑1 (MQ=255)                             gCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCttt                                                                                                                    <  2:574583/149‑1 (MQ=255)                               tGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCg                                                                                                                  >  4:443911/1‑149 (MQ=255)                                   cAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCttt                                                                                                              <  2:158688/149‑1 (MQ=255)                                   cAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCttt                                                                                                              <  3:21614/149‑1 (MQ=255)                                     gTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCa                                                                                                            <  1:493713/149‑1 (MQ=255)                                     gTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCa                                                                                                            <  4:538408/149‑1 (MQ=255)                                            gCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACttt                                                                                                     <  1:527308/149‑1 (MQ=255)                                             ccAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACtttt                                                                                                    >  4:133001/1‑149 (MQ=255)                                              cAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTg                                                                                                   >  1:309131/1‑149 (MQ=255)                                                aaGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGcc                                                                                                 >  4:66416/1‑149 (MQ=255)                                                    gATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGt                                                                                             >  4:152566/1‑149 (MQ=255)                                                       gTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCCTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGAt                                                                                          <  3:348327/149‑1 (MQ=255)                                                             gCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGaaaa                                                                                    <  3:331842/149‑1 (MQ=255)                                                               ccAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACt                                                                                  >  2:118686/1‑149 (MQ=255)                                                               ccAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACt                                                                                  >  4:14935/1‑149 (MQ=255)                                                                  gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCa                                                                               <  3:14935/149‑1 (MQ=255)                                                                        aaCCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAAccc                                                                         >  3:150159/1‑149 (MQ=255)                                                                        aaCCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAAccc                                                                         >  3:442419/1‑149 (MQ=255)                                                                         aCCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGGACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCa                                                                        <  2:351882/149‑1 (MQ=255)                                                                         aCCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCa                                                                        >  4:431819/1‑149 (MQ=255)                                                                                 cTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATg                                                                <  4:150159/149‑1 (MQ=255)                                                                                 cTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATg                                                                <  4:442419/149‑1 (MQ=255)                                                                                        aGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGcttctt                                                         >  4:126314/1‑149 (MQ=255)                                                                                         gCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCttt                                                        <  2:568289/149‑1 (MQ=255)                                                                                         gCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCttt                                                        <  4:36858/149‑1 (MQ=255)                                                                                           ttCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTca                                                      <  2:194451/149‑1 (MQ=255)                                                                                           ttCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTca                                                      <  2:309131/149‑1 (MQ=255)                                                                                             cAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTcaca                                                    <  1:5057/149‑1 (MQ=255)                                                                                             cAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTcaca                                                    <  2:181178/149‑1 (MQ=255)                                                                                               gCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGt                                                  <  4:311687/149‑1 (MQ=255)                                                                                                  aTAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTCCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGtttt                                               <  3:500005/149‑1 (MQ=255)                                                                                                  aTAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTCCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGtttt                                               <  3:102707/149‑1 (MQ=255)                                                                                                   tAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTc                                              <  3:84606/149‑1 (MQ=255)                                                                                                   tAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTc                                              >  3:192919/1‑149 (MQ=255)                                                                                                    aaCAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTcg                                             <  4:11587/149‑1 (MQ=255)                                                                                                                       ttGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCAt                          <  3:133001/149‑1 (MQ=255)                                                                                                                              ccAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTcc                   <  1:207979/149‑1 (MQ=255)                                                                                                                               cAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGTTGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCt                  >  2:156883/1‑149 (MQ=255)                                                                                                                               cAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCt                  <  3:152566/149‑1 (MQ=255)                                                                                                                                aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                 >  1:122638/1‑149 (MQ=255)                                                                                                                                aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                 <  3:342100/149‑1 (MQ=255)                                                                                                                                aGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTg                 >  3:19974/1‑149 (MQ=255)                                                                                                                                         gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAc        <  3:66416/149‑1 (MQ=255)                                                                                                                                         gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAc        <  3:431819/149‑1 (MQ=255)                                                                                                                                         gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAc        <  1:149075/149‑1 (MQ=255)                                                                                                                                         gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAc        <  3:126314/149‑1 (MQ=255)                                                                                                                                            tAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgtt     >  1:147266/1‑149 (MQ=255)                                                                                                                                              gCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgt   >  3:287550/1‑149 (MQ=255)                                                                                                                                               ccACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACgttgtt  >  1:292404/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                               GTATTTACCATCTTTGTAGAATTCAGAAGCTGCGCAGTCCATCGCCAAAGTGATGTCTTTGCCCAGTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTT  >  NC_000913/2907168‑2907458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑