Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	394,892	C→T	9.0%	R129R (CGC→CGT)	yaiV →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	394,892	0	C	T	9.0%	201.4 / 8.7	67	R129R (CGC→CGT)	yaiV	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base T (3/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TATACCATACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCG  >  NC_000913/394748‑395034                                                                                                                                                 |                                                                                                                                               tataCCATACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACg                                                                                                                                            >  1:35454/1‑149 (MQ=255)    aCCATACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCGACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGc                                                                                                                                         <  3:360997/149‑1 (MQ=255)       aTACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGc                                                                                                                                      >  1:216670/1‑149 (MQ=255)       aTACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGc                                                                                                                                      >  3:234794/1‑149 (MQ=255)       aTACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGc                                                                                                                                      >  2:28044/1‑149 (MQ=255)       aTACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGc                                                                                                                                      <  1:329343/149‑1 (MQ=255)        tACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACTCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTAGGCTACGTGCAGCt                                                                                                                                     >  2:520490/1‑149 (MQ=255)        tACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCt                                                                                                                                     >  1:227693/1‑149 (MQ=255)           aaaTTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCAt                                                                                                                                  >  4:30700/1‑149 (MQ=255)           aaaTTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCAt                                                                                                                                  >  2:244766/1‑149 (MQ=255)            aaTTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCAtt                                                                                                                                 >  3:423186/1‑149 (MQ=255)            aaTTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCAtt                                                                                                                                 <  3:59659/149‑1 (MQ=255)                    tCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATaa                                                                                                                         <  3:165616/149‑1 (MQ=255)                     cAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAAt                                                                                                                        <  2:112884/149‑1 (MQ=255)                       gAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTc                                                                                                                      >  1:560904/1‑149 (MQ=255)                        aaGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTcc                                                                                                                     >  3:358808/1‑149 (MQ=255)                          ggAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTa                                                                                                                   <  1:185095/149‑1 (MQ=255)                           gAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTAc                                                                                                                  <  1:453383/149‑1 (MQ=255)                             aaTTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGa                                                                                                                >  3:528301/1‑149 (MQ=255)                               ttGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAAc                                                                                                              >  4:194326/1‑149 (MQ=255)                                  tACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACaaa                                                                                                           >  1:99011/1‑149 (MQ=255)                                   aCGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAAt                                                                                                          <  2:36068/149‑1 (MQ=255)                                     gggATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATcc                                                                                                        >  4:407330/1‑149 (MQ=255)                                       gATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCcgc                                                                                                      >  1:471389/1‑149 (MQ=255)                                             atcTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACa                                                                                                >  4:376666/1‑149 (MQ=255)                                             atcTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACa                                                                                                >  4:175407/1‑149 (MQ=255)                                                tACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGc                                                                                                                             >  3:91765/1‑117 (MQ=255)                                                tACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGc                                                                                                                             <  4:91765/117‑1 (MQ=255)                                                tACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCaa                                                                                                  >  4:273121/1‑144 (MQ=255)                                                tACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCaa                                                                                                  <  3:273121/144‑1 (MQ=255)                                                 aCCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACattat                                                                                            >  1:325718/1‑149 (MQ=255)                                                 aCCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACattat                                                                                            <  4:187055/149‑1 (MQ=255)                                                    aGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAAAAAATCCGCGCAACATtattat                                                                                         >  2:412543/1‑149 (MQ=255)                                                     gCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATc                                                                                        <  3:468711/149‑1 (MQ=255)                                                         aacaaacCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATg                                                                                    >  4:426920/1‑149 (MQ=255)                                                          acaaacCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGa                                                                                   <  4:423186/149‑1 (MQ=255)                                                                    aCGCTTATTGTACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATACGCGCAACATTATTATCAATGATTGACTGGaa                                                                         <  4:362218/149‑1 (MQ=255)                                                                    aCGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGaa                                                                         <  1:430646/149‑1 (MQ=255)                                                                        ttATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATgaa                                                                     <  2:325718/149‑1 (MQ=255)                                                                        ttATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATgaa                                                                     <  3:269199/149‑1 (MQ=255)                                                                        ttATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATgaa                                                                     <  2:471389/149‑1 (MQ=255)                                                                              aaCAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTg                                                               >  3:100961/1‑149 (MQ=255)                                                                                       aaCTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGt                                                      >  1:238821/1‑149 (MQ=255)                                                                                          tctGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTAtt                                                   <  1:223371/149‑1 (MQ=255)                                                                                           ctGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTg                                                  <  3:28080/149‑1 (MQ=255)                                                                                           ctGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTg                                                  <  4:528301/149‑1 (MQ=255)                                                                                             ggCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGgt                                                <  1:493994/149‑1 (MQ=255)                                                                                                   aCGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGtgatg                                          >  3:338793/1‑149 (MQ=255)                                                                                                   aCGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGtgatg                                          >  3:168245/1‑149 (MQ=255)                                                                                                     gCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGaa                                        >  2:385982/1‑149 (MQ=255)                                                                                                     gCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGaa                                        >  2:223324/1‑149 (MQ=255)                                                                                                     gCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGaa                                        >  2:370637/1‑149 (MQ=255)                                                                                                       tttttACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAAtt                                      <  3:194326/149‑1 (MQ=255)                                                                                                         tttACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATtat                                    <  4:106313/149‑1 (MQ=255)                                                                                                           tACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTatat                                  <  2:35454/149‑1 (MQ=255)                                                                                                            aCTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATc                                 <  2:227693/149‑1 (MQ=255)                                                                                                              tGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCa                               >  2:469572/1‑149 (MQ=255)                                                                                                                                  aaTTCTGGAATTACGCGACGTGCAGTTCATTGGGAATAATTCCAACGAACAAATCCGCGCAACATTATTATAAATGATTGACTGAAATGAAGATTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATACcgc           <  4:358808/149‑1 (MQ=255)                                                                                                                                  aaTTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATcgc           <  3:30700/149‑1 (MQ=255)                                                                                                                                     tCTGGAATTGCGCGACGTGCAGCTCATTAGGCATAATTCCTACGAACAAGTCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCTATCAACGTACACGCATATCGCGtt        <  3:355130/149‑1 (MQ=255)                                                                                                                                      cTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTc       >  3:270242/1‑149 (MQ=255)                                                                                                                                       tGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCt      >  2:431269/1‑149 (MQ=255)                                                                                                                                        ggAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTg     <  4:234794/149‑1 (MQ=255)                                                                                                                                          aaTTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTgtc   <  1:370637/149‑1 (MQ=255)                                                                                                                                          aaTTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTgtc   <  1:385982/149‑1 (MQ=255)                                                                                                                                          aaTTACGTGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTgtc   <  1:223324/149‑1 (MQ=255)                                                                                                                                           aTTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTgtcg  >  1:584716/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                               TATACCATACAAATTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTTATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATTCTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCG  >  NC_000913/394748‑395034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑