Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,299,574	C→A	7.1%	P114P (CCG→CCT)	yhbP ←	UPF0306 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,574	0	C	A	7.1%	191.4 / 5.1	56	P114P (CCG→CCT)	yhbP	UPF0306 family protein
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base A (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

CCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTC  >  NC_000913/3299428‑3299722                                                                                                                                                    |                                                                                                                                                     ccAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCgg                                                                                                                                                    <  4:441790/149‑1 (MQ=255)       ggACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGa                                                                                                                                              <  1:158228/149‑1 (MQ=255)           acCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTAGGTGAGTTTGATTTCATCGAGCCGCATGTCCCGCACCGGGGCCGACAGc                                                                                                                                          >  4:37916/1‑149 (MQ=255)             cGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACAGGGGCCGACAGCAt                                                                                                                                        >  2:272089/1‑149 (MQ=255)             cGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTATACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACAGGGGCCGACAGCAt                                                                                                                                        >  4:371416/1‑149 (MQ=255)                    tCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCa                                                                                                                                 >  4:65856/1‑149 (MQ=255)                            cGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGTAACCGGaaa                                                                                                                         <  4:217411/149‑1 (MQ=255)                            cGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGaaa                                                                                                                         <  4:84523/149‑1 (MQ=255)                             gTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAg                                                                                                                        <  2:307340/149‑1 (MQ=255)                              tttAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTTGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGc                                                                                                                       >  3:26009/1‑149 (MQ=255)                                  aCCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgacg                                                                                                                   >  1:278612/1‑149 (MQ=255)                                  aCCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgacg                                                                                                                   <  3:480001/149‑1 (MQ=255)                                  aCCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgacg                                                                                                                   <  3:67649/149‑1 (MQ=255)                                   ccGCCAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGcgacga                                                                                                                  >  4:172245/1‑149 (MQ=255)                                       gAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGt                                                                                                              <  3:126031/149‑1 (MQ=255)                                           cGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGc                                                                                                          <  4:412568/149‑1 (MQ=255)                                            gTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGcc                                                                                                         <  3:172245/149‑1 (MQ=255)                                               aCGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTg                                                                                                      >  4:181181/1‑149 (MQ=255)                                                    tGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTgcgcgc                                                                                                 >  3:194253/1‑149 (MQ=255)                                                                gAGTCGCGTAGCCAAATCATTTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCttt                                                                                      >  4:271718/1‑149 (MQ=255)                                                                gAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTc                                                                                     <  1:474835/149‑1 (MQ=255)                                                                     gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGTCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTc                                                                                     >  1:520894/1‑144 (MQ=255)                                                                     gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTc                                                                                     <  2:520894/144‑1 (MQ=255)                                                                     gcgTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCAc                                                                                >  1:192228/1‑149 (MQ=255)                                                                            cAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCTAg                                                                         >  3:500580/1‑149 (MQ=255)                                                                                tCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTg                                                                     <  4:236961/149‑1 (MQ=255)                                                                                    tttttttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCgg                                                                  >  3:252913/1‑149 (MQ=255)                                                                                      tttttACCAAAGCCCAGCGTGTTGTTGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACAGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAt                                                                <  3:371416/149‑1 (MQ=255)                                                                                      tttttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACAGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAt                                                                <  1:272089/149‑1 (MQ=255)                                                                                        tttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtct                                                              <  3:524646/149‑1 (MQ=255)                                                                                          tACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCa                                                            <  3:226591/149‑1 (MQ=255)                                                                                             cAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCa                                                            <  4:77088/146‑1 (MQ=255)                                                                                             cAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCa                                                            >  3:77088/1‑146 (MQ=255)                                                                                             cAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCt                                                         >  3:522333/1‑149 (MQ=255)                                                                                              aaaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt                                                        >  1:521895/1‑149 (MQ=255)                                                                                               aaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCttt                                                       >  3:30190/1‑149 (MQ=255)                                                                                                aGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCTCACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTa                                                      >  2:9480/1‑149 (MQ=255)                                                                                                     aGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCAACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTg                                                 <  4:32120/149‑1 (MQ=255)                                                                                                          gttgtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCacac                                            <  4:26009/149‑1 (MQ=255)                                                                                                             gtCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACcgc                                         <  2:498069/149‑1 (MQ=255)                                                                                                               cGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGa                                       <  4:30190/149‑1 (MQ=255)                                                                                                                 gTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAAt                                     <  2:556368/149‑1 (MQ=255)                                                                                                                    aaTTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTaa                                  >  4:79841/1‑149 (MQ=255)                                                                                                                    aaTTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTaa                                  <  1:358149/149‑1 (MQ=255)                                                                                                                     aTTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAAc                                 <  2:370345/149‑1 (MQ=255)                                                                                                                      tttGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAAc                                 >  3:12008/1‑148 (MQ=255)                                                                                                                      tttGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAAc                                 <  4:12008/148‑1 (MQ=255)                                                                                                                      tttGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACg                                >  1:476344/1‑149 (MQ=255)                                                                                                                           tttCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTAcc                           >  1:47407/1‑149 (MQ=255)                                                                                                                                tCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGtttt                      >  2:472702/1‑149 (MQ=255)                                                                                                                                   aGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCgg                   >  1:269196/1‑149 (MQ=255)                                                                                                                                     ccGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCt                 <  4:194253/149‑1 (MQ=255)                                                                                                                                           ttCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCAcc                                                                               >  1:149575/1‑81 (MQ=255)                                                                                                                                           ttCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCAcc                                                                               <  2:149575/81‑1 (MQ=255)                                                                                                                                              ccACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTa        >  1:17090/1‑149 (MQ=255)                                                                                                                                                acacCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTAcc      >  1:288558/1‑149 (MQ=255)                                                                                                                                                    cGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTc  >  3:413796/1‑149 (MQ=255)                                                                                                                                                    cGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTc  >  1:137576/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACTGCACACCGCGAATTAACGCTACCGTTTTCGGCTGACCGTTTACCGTTC  >  NC_000913/3299428‑3299722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑