Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,478,932 A→G 6.5% L559P (CTC→CCC)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,478,9320AG 6.5% 187.5 / 3.8 61L559P (CTC→CCC) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (23/34);  new base G (2/2);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGC  >  NC_000913/3478784‑3479080
                                                                                                                                                    |                                                                                                                                                    
gATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGa                                                                                                                                                      <  4:46893/149‑1 (MQ=255)
gATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGa                                                                                                                                                      >  4:13579/1‑149 (MQ=255)
 aTTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAg                                                                                                                                                     <  2:2525/149‑1 (MQ=255)
   tGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGGGct                                                                                                                                                   >  2:212748/1‑149 (MQ=255)
   tGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGGGct                                                                                                                                                   >  2:162201/1‑149 (MQ=255)
    gCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctc                                                                                                                                                  <  4:11289/149‑1 (MQ=255)
    gCCATCTTTACCCTCTACTCAAATTCACCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCACTTCACGCTGGAAAATCTCTTCCAGTCCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCAGCATGCATTGGGATGAGctc                                                                                                                                                  <  4:132286/149‑1 (MQ=255)
     ccATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAGGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct                                                                                                                                                 <  3:128613/149‑1 (MQ=255)
     ccATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct                                                                                                                                                 >  2:518742/1‑149 (MQ=255)
     ccATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct                                                                                                                                                 >  3:261407/1‑149 (MQ=255)
     ccATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGctct                                                                                                                                                 >  2:206483/1‑149 (MQ=255)
      cATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCtctc                                                                                                                                                >  1:403295/1‑149 (MQ=255)
      cATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCtctc                                                                                                                                                >  1:325885/1‑149 (MQ=255)
         cTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCgca                                                                                                                                             <  4:230805/149‑1 (MQ=255)
          tttACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCgcag                                                                                                                                            >  3:51641/1‑149 (MQ=255)
               ccTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATcc                                                                                                                                       >  2:521560/1‑149 (MQ=255)
               ccTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATcc                                                                                                                                       >  3:423992/1‑149 (MQ=255)
                      tCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATc                                                                                                                                >  3:507513/1‑149 (MQ=255)
                      tCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATc                                                                                                                                >  2:452056/1‑149 (MQ=255)
                      tCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATc                                                                                                                                >  2:364982/1‑149 (MQ=255)
                       cAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATcc                                                                                                                               <  4:189774/149‑1 (MQ=255)
                         aaTTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAg                                                                                                                             <  1:259854/149‑1 (MQ=255)
                               tCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGgcggcg                                                                                                                       >  1:46525/1‑149 (MQ=255)
                                cccAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGa                                                                                                                      <  3:178727/149‑1 (MQ=255)
                                   aGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGaaaa                                                                                                                   <  4:51641/149‑1 (MQ=255)
                                               ggCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGAGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGcgct                                                                                                       >  1:412355/1‑149 (MQ=255)
                                                            ttgCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGAt                                                                                          <  1:452056/149‑1 (MQ=255)
                                                              gCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGc                                                                                        <  4:196859/149‑1 (MQ=255)
                                                               cATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCg                                                                                       >  2:132600/1‑149 (MQ=255)
                                                                aTTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAACTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCgg                                                                                      <  2:228898/149‑1 (MQ=255)
                                                                 tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGa                                                                                     <  3:309549/149‑1 (MQ=255)
                                                                 tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGa                                                                                     >  4:549146/1‑149 (MQ=255)
                                                                 tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGa                                                                                     <  1:268152/149‑1 (MQ=255)
                                                                 tttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGa                                                                                     <  2:403295/149‑1 (MQ=255)
                                                                  ttCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGat                                                                                    <  4:394004/149‑1 (MQ=255)
                                                                           gAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGc                                                                                               <  1:156085/129‑1 (MQ=255)
                                                                           gAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGc                                                                                               >  2:156085/1‑129 (MQ=255)
                                                                             aaaTCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGc                                                                         >  2:403866/1‑149 (MQ=255)
                                                                             aaaTCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGc                                                                         <  1:403866/149‑1 (MQ=255)
                                                                                 ctctTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGa                                                                     <  4:272939/149‑1 (MQ=255)
                                                                                  tctTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGAc                                                                    >  3:167698/1‑149 (MQ=255)
                                                                                    ttCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCa                                                                  >  3:466410/1‑149 (MQ=255)
                                                                                           tCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCtt                                                           <  4:267805/149‑1 (MQ=255)
                                                                                           tCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCtt                                                           <  1:187821/149‑1 (MQ=255)
                                                                                            cgcgTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTg                                                          <  2:569907/149‑1 (MQ=255)
                                                                                                 cGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGccc                                                     <  1:26649/149‑1 (MQ=255)
                                                                                                    gCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAg                                                  <  3:549146/149‑1 (MQ=255)
                                                                                                           tGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGGGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAAc                                           <  1:162201/149‑1 (MQ=255)
                                                                                                           tGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGGGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAAc                                           <  1:212748/149‑1 (MQ=255)
                                                                                                           tGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCAAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAAc                                           <  2:224729/149‑1 (MQ=255)
                                                                                                                      aTTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGaa                                <  1:518742/149‑1 (MQ=255)
                                                                                                                             cGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCa                         <  3:144900/149‑1 (MQ=255)
                                                                                                                              ggTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCAc                        <  2:241099/149‑1 (MQ=255)
                                                                                                                              ggTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCAc                        <  4:512353/149‑1 (MQ=255)
                                                                                                                               gTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACg                       <  2:446514/149‑1 (MQ=255)
                                                                                                                                       atgcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACt               >  4:318511/1‑149 (MQ=255)
                                                                                                                                         gcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGc             <  4:507513/149‑1 (MQ=255)
                                                                                                                                         gcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGc             <  1:111978/149‑1 (MQ=255)
                                                                                                                                         gcatTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTCATGCGGATGCACGCCAAGCGTGACCAGCGTCTGGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACGGc             <  2:480795/149‑1 (MQ=255)
                                                                                                                                           atTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGt           >  4:183249/1‑149 (MQ=255)
                                                                                                                                               ggATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCAcc       <  3:207705/149‑1 (MQ=255)
                                                                                                                                                gATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCAccc      >  4:106599/1‑149 (MQ=255)
                                                                                                                                                gATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCAccc      >  4:446135/1‑149 (MQ=255)
                                                                                                                                                    aGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCcctgc  <  1:234428/149‑1 (MQ=255)
                                                                                                                                                    aGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCcctgc  >  2:361791/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCTCGCAGCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGC  >  NC_000913/3478784‑3479080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑