Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,632,645 G→T 6.2% intergenic (‑55/+207) yhiJ ← / ← yhiL DUF4049 family protein/pseudogene, DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,6450GT 6.2% 219.8 / 3.8 65intergenic (‑55/+207)yhiJ/yhiLDUF4049 family protein/pseudogene, DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base G (24/36);  new base T (2/2);  total (26/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAT  >  NC_000913/3632497‑3632791
                                                                                                                                                    |                                                                                                                                                  
cTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATg                                                                                                                                                    >  3:401745/1‑149 (MQ=255)
   tAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACa                                                                                                                                                 <  1:94485/149‑1 (MQ=255)
     gTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATa                                                                                                                                               >  2:99060/1‑149 (MQ=255)
     gTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATAACATa                                                                                                                                               <  4:255641/149‑1 (MQ=255)
             ggTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCAt                                                                                                                                       >  1:551257/1‑149 (MQ=255)
             ggTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCAt                                                                                                                                       >  2:516351/1‑149 (MQ=255)
             ggTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCAt                                                                                                                                       <  2:312983/149‑1 (MQ=255)
                aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAAATTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGtt                                                                                                                                    >  2:145612/1‑149 (MQ=255)
                    tGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGAt                                                                                                                                <  4:80761/149‑1 (MQ=255)
                    tGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGAt                                                                                                                                <  4:100511/149‑1 (MQ=255)
                      ctctTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATgg                                                                                                                              <  3:221581/149‑1 (MQ=255)
                       tctTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATggg                                                                                                                             >  3:71558/1‑149 (MQ=255)
                              ccATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAaataa                                                                                                                      >  2:81205/1‑149 (MQ=255)
                               cATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTACCTTATGACATACACCTCATGTTCGATGGGAAaataat                                                                                                                     <  3:373719/149‑1 (MQ=255)
                                  atCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATtat                                                                                                                  <  3:194760/149‑1 (MQ=255)
                                     aTTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATAtt                                                                                                               >  2:210761/1‑149 (MQ=255)
                                     aTTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATAtt                                                                                                               >  2:524686/1‑149 (MQ=255)
                                      tttGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATAttt                                                                                                              >  3:556117/1‑149 (MQ=255)
                                        tgttgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTg                                                                                                            <  3:552530/149‑1 (MQ=255)
                                        tgttgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTg                                                                                                            <  3:265839/149‑1 (MQ=255)
                                          ttgCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGca                                                                                                          <  4:52380/149‑1 (MQ=255)
                                             cGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACaa                                                                                                       <  4:295259/149‑1 (MQ=255)
                                               aTTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAAt                                                                                                     >  3:438935/1‑149 (MQ=255)
                                                ttGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATc                                                                                                    <  1:516351/149‑1 (MQ=255)
                                                  gTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCAt                                                                                                  <  2:462176/149‑1 (MQ=255)
                                                   tCGTTGTACTGTCGTTAGTGCCTGCCTCAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCAtt                                                                                                 <  4:557756/149‑1 (MQ=255)
                                                    cGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTc                                                                                                <  3:547483/149‑1 (MQ=255)
                                                    cGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTc                                                                                                <  2:373680/149‑1 (MQ=255)
                                                     gTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCa                                                                                               <  1:559811/149‑1 (MQ=255)
                                                     gTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCa                                                                                               >  1:302437/1‑149 (MQ=255)
                                                      ttGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAg                                                                                              >  1:427837/1‑149 (MQ=255)
                                                      ttGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAg                                                                                              >  1:294751/1‑149 (MQ=255)
                                                                 ttAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCat                                                                                   <  2:427837/149‑1 (MQ=255)
                                                                 ttAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCat                                                                                   <  2:294751/149‑1 (MQ=255)
                                                                        ctgccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGa                                                                            <  1:352261/149‑1 (MQ=255)
                                                                          gccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGaaa                                                                          <  4:230237/149‑1 (MQ=255)
                                                                          gccACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGaaa                                                                          >  4:131312/1‑149 (MQ=255)
                                                                                        tttCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATa                                                            <  3:133552/149‑1 (MQ=255)
                                                                                        tttCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATa                                                            <  4:401745/149‑1 (MQ=255)
                                                                                         ttCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAg                                                           <  1:533222/149‑1 (MQ=255)
                                                                                         ttCATATTTTACCTCATATTATTTACCGCGAGAAATACATAGTTAACATTATATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAg                                                           <  1:36317/149‑1 (MQ=255)
                                                                                             tatTTTACTTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCaga                                                       >  3:549518/1‑149 (MQ=255)
                                                                                             tatTTTACCTCATATTATTTACCGCGCGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCaga                                                       <  1:145612/149‑1 (MQ=255)
                                                                                                  tACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAg                                                  >  1:248289/1‑149 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATTACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               >  3:476989/1‑149 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATTACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               >  3:49648/1‑149 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               >  1:582714/1‑149 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               <  2:396824/149‑1 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               >  4:226394/1‑149 (MQ=255)
                                                                                                     cTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTcc                                               >  3:341248/1‑149 (MQ=255)
                                                                                                      tCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTccc                                              <  3:96644/149‑1 (MQ=255)
                                                                                                      tCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTccc                                              <  4:71558/149‑1 (MQ=255)
                                                                                                       cATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCt                                             <  3:362198/149‑1 (MQ=255)
                                                                                                              tttACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGAtt                                      <  3:131312/149‑1 (MQ=255)
                                                                                                               ttACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTg                                     <  3:142240/149‑1 (MQ=255)
                                                                                                                      gAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGc                              <  2:248289/149‑1 (MQ=255)
                                                                                                                         gAATACATAGTTAACATTTTATCTTATTACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtc                           <  4:476989/149‑1 (MQ=255)
                                                                                                                         gAATACATAGTTAACATTTTATCTTATTACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtc                           <  4:49648/149‑1 (MQ=255)
                                                                                                                              cATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAg                      >  1:9485/1‑149 (MQ=255)
                                                                                                                                   ttAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACAt                 >  2:275732/1‑149 (MQ=255)
                                                                                                                                    tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATg                >  3:346152/1‑149 (MQ=255)
                                                                                                                                        aTTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCctc            >  3:312701/1‑149 (MQ=255)
                                                                                                                                             aTCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACaa       >  4:188405/1‑149 (MQ=255)
                                                                                                                                                 tATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGa   <  4:438935/149‑1 (MQ=255)
                                                                                                                                                  aTGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAt  <  2:302437/149‑1 (MQ=255)
                                                                                                                                                  aTGACATACAACTCATCTTCGATGAAAAAATAAATATATTTTGCACAAATCATTCAGACTCAAAGCATAATATGAAATTTCTTATCTCATAGCACAGAAAGTCCCTAAAGAATGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAt  <  2:464888/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAT  >  NC_000913/3632497‑3632791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑