Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,912,161 A→G 6.1% L503P (CTG→CCG)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,912,1610AG 6.1% 205.0 / 4.2 65L503P (CTG→CCG) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (36/25);  new base G (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

ATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCA  >  NC_000913/3912016‑3912304
                                                                                                                                                 |                                                                                                                                               
aTCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGtt                                                                                                                                              >  1:325121/1‑149 (MQ=255)
 tCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTc                                                                                                                                             <  2:429194/149‑1 (MQ=255)
     gATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCca                                                                                                                                         >  1:367311/1‑149 (MQ=255)
       tCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCcagc                                                                                                                                       >  4:309156/1‑149 (MQ=255)
            gAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGt                                                                                                                                  >  2:20004/1‑149 (MQ=255)
            gAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGt                                                                                                                                  >  3:548318/1‑149 (MQ=255)
                  aTACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCtt                                                                                                                            >  1:432747/1‑149 (MQ=255)
                  aTACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATCTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACAGGCATATCGGCATCAATTAGCGCCAGCGTACCGTGTTTCAGTTCGCCAGCAGCGTAGGCtt                                                                                                                            >  4:93813/1‑149 (MQ=255)
                   tACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCGGTTCGCCAGCAGCGTGGGCTTc                                                                                                                           >  4:72139/1‑149 (MQ=255)
                   tACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCGGTTCGCCAGCAGCGTAGGCTTc                                                                                                                           >  2:364310/1‑149 (MQ=255)
                   tACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTc                                                                                                                           >  2:214236/1‑149 (MQ=255)
                      aaCTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGc                                                                                                                        >  3:488653/1‑149 (MQ=255)
                             cGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATg                                                                                                                 >  1:134290/1‑149 (MQ=255)
                                cACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTaa                                                                                                              <  1:185820/149‑1 (MQ=255)
                                  cgcgcgcgAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAaga                                                                                                            <  2:399380/149‑1 (MQ=255)
                                    cgcgcgAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAgaga                                                                                                          <  4:548318/149‑1 (MQ=255)
                                    cgcgcgAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAgaga                                                                                                          <  3:358360/149‑1 (MQ=255)
                                    cgcgcgAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAgaga                                                                                                          <  2:275099/149‑1 (MQ=255)
                                         gAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATctct                                                                                                     <  2:148369/149‑1 (MQ=255)
                                             ttcttcAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCa                                                                                                 >  2:395021/1‑149 (MQ=255)
                                             ttcttcAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCa                                                                                                 >  3:269840/1‑149 (MQ=255)
                                                 tcAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACtt                                                                                             >  2:113924/1‑149 (MQ=255)
                                                       ttGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCaa                                                                                                >  3:509692/1‑140 (MQ=255)
                                                       ttGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCaa                                                                                                <  4:509692/140‑1 (MQ=255)
                                                         ggATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATgcgc                                                                                     >  3:9402/1‑149 (MQ=255)
                                                             ttCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTc                                                                                 >  3:379890/1‑149 (MQ=255)
                                                              tCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTcc                                                                                <  4:213187/149‑1 (MQ=255)
                                                                 gTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAgc                                                                             >  2:255283/1‑149 (MQ=255)
                                                                       ccAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGAtt                                                                       <  1:56824/149‑1 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAAt                                                                                                                  >  3:529382/1‑104 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAAt                                                                                                                  <  4:529382/104‑1 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTgg                                                                     >  2:137810/1‑149 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTgg                                                                     >  1:549667/1‑149 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTgg                                                                     <  3:309156/149‑1 (MQ=255)
                                                                         aGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCATTCCAGCGCGATTgg                                                                     <  3:470076/149‑1 (MQ=255)
                                                                           cAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGt                                                                   >  1:115268/1‑149 (MQ=255)
                                                                                 gttgttCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGAt                                                             <  3:349352/149‑1 (MQ=255)
                                                                                 gttgttCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGAt                                                             >  1:317546/1‑149 (MQ=255)
                                                                                  ttgttCGGTGCAACAACAATAACCGGCATAGCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATc                                                            <  1:378196/149‑1 (MQ=255)
                                                                                         gTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACgg                                                     >  4:264076/1‑149 (MQ=255)
                                                                                           gCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATgc                                                                                       <  2:26002/113‑1 (MQ=255)
                                                                                           gCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATgc                                                                                       >  1:26002/1‑113 (MQ=255)
                                                                                            caacaacaaTAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGccc                                                  >  1:113516/1‑149 (MQ=255)
                                                                                             aacaacaaTAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCa                                                 >  2:487340/1‑149 (MQ=255)
                                                                                             aacaacaaTAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCa                                                 >  1:371059/1‑149 (MQ=255)
                                                                                                aacaaTAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGa                                              >  1:167446/1‑149 (MQ=255)
                                                                                                aacaaTAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGa                                              >  1:433386/1‑149 (MQ=255)
                                                                                                           gCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGa                                   >  4:286373/1‑149 (MQ=255)
                                                                                                                  ggCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGt                            <  4:9402/149‑1 (MQ=255)
                                                                                                                   gCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGTCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTc                           <  4:379890/149‑1 (MQ=255)
                                                                                                                      tCAATTAGCGCCAGCGGACCGTGTTTCGGTTCGCCAGCAGCGTGGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCaga                        <  3:72139/149‑1 (MQ=255)
                                                                                                                      tCAATTAGCGCCAGCGGACCGTGTTTCGGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCaga                        <  1:364310/149‑1 (MQ=255)
                                                                                                                       cAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCagag                       >  3:524118/1‑149 (MQ=255)
                                                                                                                         aTTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGaa                     >  1:44884/1‑149 (MQ=255)
                                                                                                                          ttAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGaaa                    >  3:296336/1‑149 (MQ=255)
                                                                                                                            aGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtc                  <  4:23352/149‑1 (MQ=255)
                                                                                                                              cgcCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtctt                <  2:44884/149‑1 (MQ=255)
                                                                                                                               gcCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtcttc               <  3:304027/149‑1 (MQ=255)
                                                                                                                               gcCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtcttc               <  2:491149/149‑1 (MQ=255)
                                                                                                                                ccAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAAtcttct              >  2:276572/1‑149 (MQ=255)
                                                                                                                                      gACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAg                                                <  4:383997/109‑1 (MQ=255)
                                                                                                                                      gACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAg                                                >  3:383997/1‑109 (MQ=255)
                                                                                                                                        ccGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAgcgc      <  1:284139/149‑1 (MQ=255)
                                                                                                                                           tgtTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTc   >  4:27154/1‑149 (MQ=255)
                                                                                                                                            gtTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCa  <  3:286373/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCA  >  NC_000913/3912016‑3912304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑