Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,956,465 A→G 6.9% I379V (ATC→GTC)  ilvA → l‑threonine dehydratase, biosynthetic; also known as threonine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,956,4650AG 6.9% 181.4 / 4.0 58I379V (ATC→GTC) ilvAl‑threonine dehydratase, biosynthetic; also known as threonine deaminase
Reads supporting (aligned to +/- strand):  ref base A (26/28);  new base G (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

GAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTCCATC  >  NC_000913/3956324‑3956613
                                                                                                                                             |                                                                                                                                                    
gAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGt                                                                                                                                               >  2:403924/1‑149 (MQ=255)
               cGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAg                                                                                                                                <  3:365240/149‑1 (MQ=255)
                 tGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCGTCTTTGTCGGTGTGCGCCTGAGcc                                                                                                                              >  1:205803/1‑149 (MQ=255)
                 tGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCGTCTTTGTCGGTGTGCGCCTGAGcc                                                                                                                              >  1:50355/1‑149 (MQ=255)
                 tGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGcc                                                                                                                              >  1:492219/1‑149 (MQ=255)
                     gCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCgg                                                                                                                          >  3:421451/1‑149 (MQ=255)
                      cGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGc                                                                                                                         <  4:281727/149‑1 (MQ=255)
                      cGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTCGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGc                                                                                                                         <  2:500241/149‑1 (MQ=255)
                         tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTc                                                                                                                      <  1:65051/149‑1 (MQ=255)
                         tgttgGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTc                                                                                                                      <  4:143273/149‑1 (MQ=255)
                                ggTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAgc                                                                                                               >  2:475227/1‑149 (MQ=255)
                                       aTTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGa                                                                                                        >  4:101949/1‑149 (MQ=255)
                                            ggAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAAttt                                                                                                   <  3:313283/149‑1 (MQ=255)
                                             gaagaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAAtttt                                                                                                  <  1:244876/149‑1 (MQ=255)
                                             gaagaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAAtttt                                                                                                  <  1:250140/149‑1 (MQ=255)
                                              aagaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTg                                                                                                 <  4:536287/149‑1 (MQ=255)
                                              aagaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTg                                                                                                 >  2:340008/1‑149 (MQ=255)
                                               agaaAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGc                                                                                                <  3:332395/149‑1 (MQ=255)
                                                          gCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAac                                                                                     >  1:371541/1‑149 (MQ=255)
                                                              ccTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAacgacg                                                                                 <  2:310477/149‑1 (MQ=255)
                                                                tCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcggc                                                                               >  3:95325/1‑149 (MQ=255)
                                                                tCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcggc                                                                               >  2:528946/1‑149 (MQ=255)
                                                                tCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGAcggc                                                                               >  3:527869/1‑149 (MQ=255)
                                                                             aCTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTgg                                                                  <  2:101960/149‑1 (MQ=255)
                                                                             aCTGCTTGGCGGGCGTTCGGTCACCGAGTTATACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGTCCCGGCCTCGAAGAGAGCAAAGAAATTTTGCAGCTGCTCACCGACGGCGGCTACAGCGTgg                                                                  >  3:30893/1‑149 (MQ=255)
                                                                                  ttGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCGTCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAt                                                             <  2:205803/149‑1 (MQ=255)
                                                                                  ttGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCGTCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAt                                                             <  2:50355/149‑1 (MQ=255)
                                                                                  ttGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAt                                                             <  4:567660/149‑1 (MQ=255)
                                                                                  ttGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAt                                                             <  4:140006/149‑1 (MQ=255)
                                                                                       gggcgTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAtctctc                                                        <  3:409417/149‑1 (MQ=255)
                                                                                       gggcgTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAtctctc                                                        <  4:367010/149‑1 (MQ=255)
                                                                                           gTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAtctc                                                          <  1:357467/143‑1 (MQ=255)
                                                                                           gTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGAtctc                                                          >  2:357467/1‑143 (MQ=255)
                                                                                              cGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCcgacgac                                                 >  2:168792/1‑149 (MQ=255)
                                                                                                 tCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTTAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaa                                              >  2:280048/1‑149 (MQ=255)
                                                                                                 tCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaa                                              >  3:360816/1‑149 (MQ=255)
                                                                                                 tCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaa                                              >  1:446223/1‑149 (MQ=255)
                                                                                                 tCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaa                                              >  2:48846/1‑149 (MQ=255)
                                                                                                 tCACCGAGTTCAACTACCGTTTTCCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaa                                              >  4:36595/1‑149 (MQ=255)
                                                                                                  cACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGaaa                                             <  4:265274/149‑1 (MQ=255)
                                                                                                   aCCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACATCGTGGTTGATCTCTCCGACGACGAAAt                                            <  3:503300/149‑1 (MQ=255)
                                                                                                   aCCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAAt                                            >  1:502121/1‑149 (MQ=255)
                                                                                                    ccGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAAGGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATg                                           >  1:477720/1‑149 (MQ=255)
                                                                                                     cGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATgg                                          <  2:274481/149‑1 (MQ=255)
                                                                                                         ttCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGaa                                      >  4:76101/1‑149 (MQ=255)
                                                                                                          tCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAg                                     <  2:492219/149‑1 (MQ=255)
                                                                                                           cAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGc                                    <  1:51016/149‑1 (MQ=255)
                                                                                                               tACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTaca                                <  4:438033/149‑1 (MQ=255)
                                                                                                                          cGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCtata                     <  2:512604/149‑1 (MQ=255)
                                                                                                                            aTGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATg                   >  4:307966/1‑149 (MQ=255)
                                                                                                                               ccAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTc                <  4:559219/149‑1 (MQ=255)
                                                                                                                                 aaaaaCGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTcgg              >  4:537316/1‑149 (MQ=255)
                                                                                                                                   aaaCGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTcggcg            >  1:11947/1‑149 (MQ=255)
                                                                                                                                      cGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGAc         <  2:213704/149‑1 (MQ=255)
                                                                                                                                      cGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGAc         <  2:266705/149‑1 (MQ=255)
                                                                                                                                      cGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGAc         <  3:76101/149‑1 (MQ=255)
                                                                                                                                          tGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTcc     >  2:341985/1‑149 (MQ=255)
                                                                                                                                           gCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTCCa    >  1:318681/1‑149 (MQ=255)
                                                                                                                                             aTCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTCCATc  >  1:538547/1‑149 (MQ=255)
                                                                                                                                             aTCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTCCATc  >  3:408234/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GAACTGGGCGAACAGCGTGAAGCGTTGTTGGCGGTGACCATTCCGGAAGAAAAAGGCAGCTTCCTCAAATTCTGCCAACTGCTTGGCGGGCGTTCGGTCACCGAGTTCAACTACCGTTTTGCCGATGCCAAAAACGCCTGCATCTTTGTCGGTGTGCGCCTGAGCCGCGGCCTCGAAGAGCGCAAAGAAATTTTGCAGATGCTCAACGACGGCGGCTACAGCGTGGTTGATCTCTCCGACGACGAAATGGCGAAGCTACACGTGCGCTATATGGTCGGCGGACGTCCATC  >  NC_000913/3956324‑3956613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑