Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,443,212 C→T 5.9% D277D (GAC→GAT)  tamA → translocation and assembly module for autotransporter export, outer membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,443,2120CT 5.9% 221.9 / 3.1 68D277D (GAC→GAT) tamAtranslocation and assembly module for autotransporter export, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base T (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CCGACTTTCTGCTACCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAAC  >  NC_000913/4443065‑4443357
                                                                                                                                                   |                                                                                                                                                 
ccgACTTTCTGCTACCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGTCg                                                                                                                                                  >  3:500472/1‑149 (MQ=255)
  gACTTTCTGCTACTGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTg                                                                                                                                                >  4:253842/1‑149 (MQ=255)
  gACTTTCTGCTACCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTg                                                                                                                                                >  2:50088/1‑149 (MQ=255)
  gACTTTCTGCTACCGGCTGGGTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTg                                                                                                                                                >  2:71313/1‑149 (MQ=255)
   aCTTTCTGCTACCGGCTTGTTTAGCGCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAGCGAGAGTATTACCATTGACGGGCGTGGTTTCGCCGCTAACAGAAAACGCCATCGAAACCGGGGTCGGTTACTCTACGGACGTgg                                                                                                                                               <  4:335625/149‑1 (MQ=255)
           cTACCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACcgcgc                                                                                                                                       >  4:24010/1‑149 (MQ=255)
             aCCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGt                                                                                                                                     >  3:40786/1‑149 (MQ=255)
                 gCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGaaa                                                                                                                                 >  2:430754/1‑149 (MQ=255)
                 gCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGaaa                                                                                                                                 <  1:444857/149‑1 (MQ=255)
                 gCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGaaa                                                                                                                                 <  3:496898/149‑1 (MQ=255)
                      tttAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGAc                                                                                                                            <  3:450649/149‑1 (MQ=255)
                                   tggtggCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCg                                                                                                               <  1:27131/149‑1 (MQ=255)
                                    ggtggCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGt                                                                                                              <  2:508932/149‑1 (MQ=255)
                                        gCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGAt                                                                                                          >  4:548601/1‑149 (MQ=255)
                                          tCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGa                                                                                                        >  4:244362/1‑149 (MQ=255)
                                          tCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGa                                                                                                        >  4:300152/1‑149 (MQ=255)
                                           ccACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGaa                                                                                                       >  4:112966/1‑149 (MQ=255)
                                           ccACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGaa                                                                                                       <  1:28745/149‑1 (MQ=255)
                                            cacaATTTTATAAAGCGCGCGAAACGAAAGTCTTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGTTCGGTCACTCTACGGACGTGGGTCCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      <  3:350295/149‑1 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      >  3:361937/1‑149 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      <  3:333991/149‑1 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      <  3:326153/149‑1 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      <  2:38650/149‑1 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      <  1:72789/149‑1 (MQ=255)
                                            cacaATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAAc                                                                                                      >  1:315871/1‑149 (MQ=255)
                                              caATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAActc                                                                                                    <  3:300152/149‑1 (MQ=255)
                                                aTTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAATACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCtt                                                                                                  >  4:1696/1‑149 (MQ=255)
                                                 tttGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTa                                                                                                 >  2:238297/1‑149 (MQ=255)
                                                   tGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATg                                                                                               <  1:215787/149‑1 (MQ=255)
                                                           cgcgcgAAACGTAAGTATTACCATTGACGGGCGTGGTATCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGt                                                                                       <  3:368747/149‑1 (MQ=255)
                                                             cgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCt                                                                                     <  3:24010/149‑1 (MQ=255)
                                                             cgcgAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCt                                                                                     <  4:194660/149‑1 (MQ=255)
                                                             cgcgAAACGAAAGTATTACCATTGACGGGCGTGGTGTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCt                                                                                     >  4:499066/1‑149 (MQ=255)
                                                                 aaacgaaaGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGacc                                                                                 >  4:508520/1‑149 (MQ=255)
                                                                     gaaaGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGAccacca                                                                             >  1:272375/1‑149 (MQ=255)
                                                                        aGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTa                                                                          <  4:40786/149‑1 (MQ=255)
                                                                         gTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTAc                                                                         <  1:238297/149‑1 (MQ=255)
                                                                         gTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTAc                                                                         <  2:156194/149‑1 (MQ=255)
                                                                         gTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTAc                                                                         >  1:284684/1‑149 (MQ=255)
                                                                           aTTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTa                                                                       <  2:293381/149‑1 (MQ=255)
                                                                           aTTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTa                                                                       <  3:548601/149‑1 (MQ=255)
                                                                           aTTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTa                                                                       <  1:430754/149‑1 (MQ=255)
                                                                                  ttGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTc                                                                <  3:65734/149‑1 (MQ=255)
                                                                                  ttGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTc                                                                <  1:382069/149‑1 (MQ=255)
                                                                                   tGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTcc                                                               <  4:425712/149‑1 (MQ=255)
                                                                                    gACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCcg                                                              >  3:287380/1‑149 (MQ=255)
                                                                                      cGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCcgcg                                                            <  3:244362/149‑1 (MQ=255)
                                                                                                ttCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGa                                                  >  4:495928/1‑149 (MQ=255)
                                                                                                          aCAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACtt                                        >  4:277301/1‑149 (MQ=255)
                                                                                                            aGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCa                                      >  3:499332/1‑149 (MQ=255)
                                                                                                            aGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCa                                      <  2:135966/149‑1 (MQ=255)
                                                                                                                   acCATCGAAACAGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATaa                               >  3:445079/1‑149 (MQ=255)
                                                                                                                     cATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATaaaa                             <  3:222813/149‑1 (MQ=255)
                                                                                                                      aTCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAAt                            >  3:488198/1‑149 (MQ=255)
                                                                                                                       tagataCCGGGGCCCGTTACTCTACGGACGAGGGACCGCAAGTGAAACCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCCGAACAGACCTTCCACGTTTGCTATaaaaag                           >  2:245719/6‑147 (MQ=255)
                                                                                                                       tCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                           >  2:333698/1‑149 (MQ=255)
                                                                                                                             ccGGGGTCGGTTACTCTACGGATGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                           >  4:249448/1‑143 (MQ=255)
                                                                                                                             ccGGGGTCGGTTACTCTACGGATGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                           <  3:249448/143‑1 (MQ=255)
                                                                                                                             ccGGGGTCGGTTACTCTACGGATGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                           >  4:464623/1‑143 (MQ=255)
                                                                                                                             ccGGGGTCGGTTACTCTACGGATGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATg                           <  3:464623/143‑1 (MQ=255)
                                                                                                                                  gTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTgaa                <  4:181570/149‑1 (MQ=255)
                                                                                                                                  gTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTgaa                >  1:94208/1‑149 (MQ=255)
                                                                                                                                  gTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTgaa                >  1:478414/1‑149 (MQ=255)
                                                                                                                                      gTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAAt            >  1:362305/1‑149 (MQ=255)
                                                                                                                                           tctACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACt       >  2:272324/1‑149 (MQ=255)
                                                                                                                                            ctACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTg      <  3:508520/149‑1 (MQ=255)
                                                                                                                                             tACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTgg     >  1:464092/1‑149 (MQ=255)
                                                                                                                                                ggACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAAc  >  1:276972/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGACTTTCTGCTACCGGCTGGTTTAACTCGGTGGTGGTGGCTCCACAATTTGATAAAGCGCGCGAAACGAAAGTATTACCATTGACGGGCGTGGTTTCGCCGCGAACAGAAAACACCATCGAAACCGGGGTCGGTTACTCTACGGACGTGGGACCGCGCGTGAAAGCGACGTGGAAAAAGCCGTGGATGAACTCTTATGGTCACAGTCTGACCACCAGTACTAGTATTTCCGCGCCGGAACAGACCCTCGACTTCAGCTATAAAATGCCGCTGCTGAAGAATCCACTGGAAC  >  NC_000913/4443065‑4443357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑