Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 600,179 C→T 6.7% A489V (GCG→GTG)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913600,1790CT 6.7% 189.4 / 3.4 60A489V (GCG→GTG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base C (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

GATGCGTCTGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAA  >  NC_000913/600034‑600325
                                                                                                                                                 |                                                                                                                                                  
gATGCGTCTGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATTCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTgcggc                                                                                                                                                 >  2:571289/1‑149 (MQ=255)
     gTCTGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCgctgc                                                                                                                                            >  3:461801/1‑149 (MQ=255)
        tGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTATATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTgg                                                                                                                                         <  4:347283/149‑1 (MQ=255)
            gAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGTGGCGCTGCTGGCGAt                                                                                                                                     >  2:327878/1‑149 (MQ=255)
               gTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGt                                                                                                                                  >  1:325743/1‑149 (MQ=255)
                tGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTa                                                                                                                                 <  1:226041/149‑1 (MQ=255)
                  gggCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGt                                                                                                                               >  1:523870/1‑149 (MQ=255)
                      cGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATc                                                                                                                           <  1:279691/149‑1 (MQ=255)
                             aTTTATCAGTCTGCTGATTATCCCGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATcc                                                                                                                    >  1:425169/1‑149 (MQ=255)
                             aTTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACACTGGAAGGGCAGGAAGGGGGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATcc                                                                                                                    >  3:139559/1‑149 (MQ=255)
                              tttATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGTGGCGCTGCTGGCGATCGTAGTGATCCCGATCCt                                                                                                                   >  3:140977/1‑149 (MQ=255)
                                 aTCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGAt                                                                                                                <  1:214143/149‑1 (MQ=255)
                                     gTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGc                                                                                                            >  1:465607/1‑149 (MQ=255)
                                        tgctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAGGGGCAGGAAGGGAGTCTGGTTGGCCCGTTGGCGTACACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGTTCCTGCTGGGCTAc                                                                                                         >  3:350487/1‑149 (MQ=255)
                                        tgctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGTTGTGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTAc                                                                                                         <  1:327878/149‑1 (MQ=255)
                                        tgctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTAc                                                                                                         >  1:581277/1‑149 (MQ=255)
                                        tgctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTAc                                                                                                         >  3:177320/1‑149 (MQ=255)
                                        tgctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTAc                                                                                                         >  1:150485/1‑149 (MQ=255)
                                          ctgATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTg                                                                                                       <  4:125485/149‑1 (MQ=255)
                                                 tCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGt                                                                                                <  3:477404/149‑1 (MQ=255)
                                                 tCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGCGCTACTGGATCCGt                                                                                                <  3:43119/149‑1 (MQ=255)
                                                           gTTTATCCCGATCTTCACCCTGGAAGGGCGGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTc                                                                                      >  4:512647/1‑149 (MQ=255)
                                                           gTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTc                                                                                      <  2:447044/149‑1 (MQ=255)
                                                           gTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTc                                                                                      >  4:85217/1‑149 (MQ=255)
                                                            tttATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCGAAATTcc                                                                                     >  3:308484/1‑149 (MQ=255)
                                                            tttATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTcc                                                                                     <  4:158879/149‑1 (MQ=255)
                                                              tATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGGCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGTGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTccgc                                                                                   <  4:140977/149‑1 (MQ=255)
                                                               aTCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTccgcc                                                                                  <  4:287129/149‑1 (MQ=255)
                                                               aTCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATCGGCTACTGGATCCGTGGCAAAATTccgcc                                                                                  >  2:556206/1‑149 (MQ=255)
                                                                   cGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGaa                                                                              >  4:78880/1‑149 (MQ=255)
                                                                       cTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCa                                                                          <  3:170432/149‑1 (MQ=255)
                                                                       cTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCAGTTGGCGTTCACCAAGACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCa                                                                          <  3:543945/149‑1 (MQ=255)
                                                                        ttCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAg                                                                         <  3:174989/149‑1 (MQ=255)
                                                                               tGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCg                                                                  >  1:305770/1‑149 (MQ=255)
                                                                                ggAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGc                                                                 >  2:186142/1‑149 (MQ=255)
                                                                                 gAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCt                                                                >  4:66362/1‑149 (MQ=255)
                                                                                     ggcgggAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAAt                                                            <  3:512647/145‑1 (MQ=255)
                                                                                     ggCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAAt                                                            <  2:195481/149‑1 (MQ=255)
                                                                                     ggCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAAt                                                            <  3:85217/149‑1 (MQ=255)
                                                                                         ggAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGTGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCt                                                        <  2:149773/149‑1 (MQ=255)
                                                                                         ggAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCt                                                        <  3:87604/149‑1 (MQ=255)
                                                                                            aGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCtttt                                                     <  4:510888/149‑1 (MQ=255)
                                                                                            aGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCtttt                                                     <  4:461801/149‑1 (MQ=255)
                                                                                             gggCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCttttt                                                    <  2:581277/149‑1 (MQ=255)
                                                                                               gCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATAGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGa                                                  >  4:393165/1‑149 (MQ=255)
                                                                                                 gTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGAtt                                                >  1:369250/1‑149 (MQ=255)
                                                                                                  tCTGTTTGGCCCGTTGGCGTTCACCAAGACGTATGCGATGGCGGGTGCGGCGCTGCTGGGGATCTTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTc                                               <  4:106126/149‑1 (MQ=255)
                                                                                                  tCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTc                                               <  4:177336/149‑1 (MQ=255)
                                                                                                      tttGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCgtgt                                           <  2:48148/149‑1 (MQ=255)
                                                                                                       ttGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGtt                                          <  3:459510/149‑1 (MQ=255)
                                                                                                       ttGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGtt                                          <  1:295410/149‑1 (MQ=255)
                                                                                                              gTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTatcatc                                   <  1:16346/149‑1 (MQ=255)
                                                                                                                     ttCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGtt                            >  3:99153/1‑149 (MQ=255)
                                                                                                                        aCCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCt                         >  1:513175/1‑149 (MQ=255)
                                                                                                                        aCCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCt                         >  3:416659/1‑149 (MQ=255)
                                                                                                                         ccAAAACGTATGCGATGGCGGGTGCGGCGCTGGTGGGGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTg                        >  2:96338/1‑149 (MQ=255)
                                                                                                                             aaCGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAg                    >  3:333461/1‑149 (MQ=255)
                                                                                                                                  aTGCGATGGCGGGTGCGGCGCTGCTGGCGATCATAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTg               <  2:180204/149‑1 (MQ=255)
                                                                                                                                         ggCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGc        <  1:556206/149‑1 (MQ=255)
                                                                                                                                            gggTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGa     >  4:116693/1‑149 (MQ=255)
                                                                                                                                               tGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGaaaa  <  3:116693/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATGCGTCTGTTGAAGTGGGGCCGGCGCTATTTATCAGTCTGCTGATTATCACGTTGTCGTTTATCCCGATCTTCACCCTGGAAGGGCAGGAAGGGCGTCTGTTTGGCCCGTTGGCGTTCACCAAAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAA  >  NC_000913/600034‑600325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑