Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 930,998 | C→T | 6.9% | intergenic (‑36/+87) | cydD ← / ← trxB | glutathione/cysteine ABC transporter export permease/ATPase/thioredoxin reductase, FAD/NAD(P)‑binding |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 930,998 | 0 | C | T | 6.9% | 184.6 / 3.5 | 58 | intergenic (‑36/+87) | cydD/trxB | glutathione/cysteine ABC transporter export permease/ATPase/thioredoxin reductase, FAD/NAD(P)‑binding |
Reads supporting (aligned to +/- strand): ref base C (28/26); new base T (2/2); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e-01 |
ATCAATATGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGTGCTGCCATG > NC_000913/930850‑931139 | aTCAATATGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGc > 1:554417/1‑149 (MQ=255) aaTATGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCaga > 4:274483/1‑149 (MQ=255) atatGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCagag > 3:134144/1‑149 (MQ=255) atatGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCa < 1:207840/146‑1 (MQ=255) atatGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCa > 2:207840/1‑146 (MQ=255) tatGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGc < 4:314453/149‑1 (MQ=255) gCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAAt > 2:220951/1‑149 (MQ=255) gCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGtt < 2:538478/149‑1 (MQ=255) cTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGTAGAGCAATGTTa > 4:85777/1‑149 (MQ=255) cTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGTAGAGCAATGTTa > 4:554480/1‑149 (MQ=255) tCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTAc > 3:215384/1‑149 (MQ=255) acaAAGCCCAGCAGACGAGAAATGTTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACaa > 4:36753/1‑149 (MQ=255) aGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACggg < 2:282796/149‑1 (MQ=255) aGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACggg < 1:79924/149‑1 (MQ=255) cccAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGa < 3:13401/149‑1 (MQ=255) aCGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTTACCAGCGTGTTAACTCTTTTTGACGAGATTTATTCATTGCTCGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGAAATGTTACAACGGGGAAAAAATaa < 3:83964/149‑1 (MQ=255) aCGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATaa > 4:196980/1‑149 (MQ=255) aCGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATaa > 2:392197/1‑149 (MQ=255) gagaAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAg < 3:9463/149‑1 (MQ=255) gagaAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAg > 2:420795/1‑149 (MQ=255) agaAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAgg < 3:201404/149‑1 (MQ=255) gaAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGc > 3:328468/1‑149 (MQ=255) atatTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGTAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGAc < 3:85777/149‑1 (MQ=255) atatTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGTAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGAc < 3:554480/149‑1 (MQ=255) atatTCAGCCAACGTTGGGAGATGACGCTTTGCTGCTTTATCCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGAc < 4:369640/149‑1 (MQ=255) aaCGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGGATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGc < 4:98114/149‑1 (MQ=255) gTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATg < 4:289699/149‑1 (MQ=255) agaTGACTCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCg > 2:314339/1‑149 (MQ=255) aTGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGcc < 3:55400/149‑1 (MQ=255) aTGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGcc > 3:48038/1‑149 (MQ=255) cGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCcttct < 2:131276/149‑1 (MQ=255) tttGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTa < 4:538095/149‑1 (MQ=255) aaaagagttAACCCGCTGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACt > 1:476458/8‑149 (MQ=255) gCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACtt > 3:2170/1‑149 (MQ=255) tAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTAc < 3:172834/149‑1 (MQ=255) aaCCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACt > 3:204707/1‑149 (MQ=255) gCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGAttt > 1:461444/1‑149 (MQ=255) tAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTaaaa < 2:178537/149‑1 (MQ=255) aaCTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAat < 4:288755/149‑1 (MQ=255) aCGAGATTTTTTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTATTACTGATTTGTAAAATTATTTTGCGtt > 4:488387/1‑148 (MQ=255) aTTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTa < 4:558701/149‑1 (MQ=255) tATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAAc < 4:48038/149‑1 (MQ=255) ttCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGt < 2:575176/112‑1 (MQ=255) ttCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGt > 1:575176/1‑112 (MQ=255) ttGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGa > 1:524252/1‑149 (MQ=255) cACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGt < 3:26535/149‑1 (MQ=255) ttAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGt < 1:135022/149‑1 (MQ=255) gCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTcc > 4:271309/1‑149 (MQ=255) gCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTcc > 3:37596/1‑149 (MQ=255) gCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTcc > 2:330301/1‑149 (MQ=255) cAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCg < 1:314339/149‑1 (MQ=255) aGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCaa < 4:459371/149‑1 (MQ=255) ttATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGt < 2:244355/149‑1 (MQ=255) ttATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGt > 1:244355/1‑149 (MQ=255) aTCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGtgc > 2:43183/1‑149 (MQ=255) tCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGtgct > 1:86418/1‑149 (MQ=255) gAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTATTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGTGCTGcc > 1:234176/1‑149 (MQ=255) gAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGGTTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGTGCTGcc > 4:79225/1‑149 (MQ=255) ttattTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGTGCTGCCATg > 1:261078/1‑149 (MQ=255) | ATCAATATGCCGCTCACAAAGCCCAGCAGACGAGAAATATTCAGCCAACGTTGGGAGATGACGCTTTGCTGTTTTAACCAGCGGGTTAACTCTTTTTGACGAGATTTATTCATTGCACGCTTAGCAGGTGAGTTATCAGAATTATTTGCAGAGCAATGTTACAACGGGGAAAAAATAAAGGCGACCCATAGTCGCATGGTGTCGCCTTCTTTACTTTTGTTACTGATTTGTAAAATTATTTTGCGTCAGCTAAACCATCGAGGTAGCGTTCCGCATCAAGTGCTGCCATG > NC_000913/930850‑931139 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |