Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,069,287	G→T	6.3%	L185M (CTG→ATG)	rutG ←	pyrimidine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,287	0	G	T	6.3%	217.4 / 4.3	63	L185M (CTG→ATG)	rutG	pyrimidine permease
Reads supporting (aligned to +/- strand):  ref base G (33/26);  new base T (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCA  >  NC_000913/1069143‑1069431                                                                                                                                                 |                                                                                                                                                 aGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCa                                                                                                                                              >  4:12157/1‑149 (MQ=255)   gcagcaTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAAt                                                                                                                                            >  1:564525/1‑149 (MQ=255)   gcagcaTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAAt                                                                                                                                            <  2:338846/149‑1 (MQ=255)       caTGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATacac                                                                                                                                        <  2:346135/149‑1 (MQ=255)         tGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAg                                                                                                                                      <  4:271588/149‑1 (MQ=255)         tGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAg                                                                                                                                      <  2:132635/149‑1 (MQ=255)                cGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTc                                                                                                                               <  2:93662/149‑1 (MQ=255)                    agTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTa                                                                                                                           <  3:431305/149‑1 (MQ=255)                      tAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATacac                                                                                                                                        <  1:2576/134‑1 (MQ=255)                      tAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATacac                                                                                                                                        >  2:2576/1‑134 (MQ=255)                         aaTCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGcc                                                                                                                      >  2:498943/1‑149 (MQ=255)                              aCGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCGTCCa                                                                                                                 <  3:463897/149‑1 (MQ=255)                                ggCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGc                                                                                                               >  4:292049/1‑149 (MQ=255)                                 gCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCt                                                                                                              <  4:360067/149‑1 (MQ=255)                                   ttttCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTAt                                                                                                            >  3:296380/1‑149 (MQ=255)                                        ccAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAg                                                                                                       >  2:565745/1‑149 (MQ=255)                                        ccAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAg                                                                                                       >  2:172094/1‑149 (MQ=255)                                        ccAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAg                                                                                                       <  2:173614/149‑1 (MQ=255)                                        ccAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAg                                                                                                       <  4:296380/149‑1 (MQ=255)                                          aGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGc                                                                                                     >  3:174288/1‑149 (MQ=255)                                              cTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGaa                                                                                                 <  3:544025/149‑1 (MQ=255)                                               tAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAg                                                                                                >  3:126871/1‑149 (MQ=255)                                               tAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAg                                                                                                >  1:363866/1‑149 (MQ=255)                                               tAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAg                                                                                                >  2:289212/1‑149 (MQ=255)                                               tAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGCAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAg                                                                                                >  1:168579/1‑149 (MQ=255)                                                 aaaCGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCATCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCGAAGGCCGAAGcc                                                                                              >  2:100243/1‑149 (MQ=255)                                                 aaaCGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCATCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGcc                                                                                              >  4:82611/1‑149 (MQ=255)                                                     gTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATa                                                                                          >  3:221357/1‑149 (MQ=255)                                                        ggTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATacac                                                                                       <  1:544791/149‑1 (MQ=255)                                                          tcatcaCGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACtt                                                                                     >  4:161078/1‑149 (MQ=255)                                                          tcatcaCGCAATACAGCGGAAAGGCGACCATTAATCCTACGAGAATCAATAACCGCTGGATCATGCTACGGGTAAACACCGCCACCAGCACACTACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACtt                                                                                     >  2:106746/1‑149 (MQ=255)                                                          tcaacaCGCCATACAGCAGACAGGCGACAATTAACCCTACTAGAATCAATAAGCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCGCAATACACAGCACCGTCATTACCGCCACCCAGCTATCACAGGCCGAAGCGGATACACtt                                                                                     <  2:522000/149‑1 (MQ=255)                                                            atcaCGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACtttt                                                                                   <  1:183124/149‑1 (MQ=255)                                                              caCGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCa                                                                                 <  3:145296/149‑1 (MQ=255)                                                              caCGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCa                                                                                 <  3:254211/149‑1 (MQ=255)                                                                 gCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTg                                                                              <  3:12157/149‑1 (MQ=255)                                                                  ccATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGc                                                                             <  1:211178/149‑1 (MQ=255)                                                                   cATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCg                                                                            >  1:45523/1‑149 (MQ=255)                                                                    aTACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGa                                                                           >  1:188458/1‑149 (MQ=255)                                                                      aCAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATa                                                                         <  1:313478/149‑1 (MQ=255)                                                                        agcagACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAgg                                                                       >  2:306176/1‑149 (MQ=255)                                                                              cAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACATTTTTCACTGCGATAGGCGCTaa                                                                 <  3:292049/149‑1 (MQ=255)                                                                              cAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTaa                                                                 <  4:373745/149‑1 (MQ=255)                                                                               aGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCATGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCTAAGCCGATACACTTTTCACTGCGATAGGCGCTAAg                                                                >  3:387811/1‑149 (MQ=255)                                                                               aGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCTCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAg                                                                >  1:381209/1‑149 (MQ=255)                                                                                   gACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCATCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCGAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCa                                                            <  1:100243/149‑1 (MQ=255)                                                                                   gACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCATCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCa                                                            <  3:82611/149‑1 (MQ=255)                                                                                   gACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCa                                                            >  3:146476/1‑149 (MQ=255)                                                                                      aaTTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGc                                                         >  4:426271/1‑149 (MQ=255)                                                                                              cTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGcc                                                 >  4:548526/1‑149 (MQ=255)                                                                                                       tCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCTATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCaccacc                                        <  1:172094/149‑1 (MQ=255)                                                                                                       tCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCaccacc                                        >  2:433512/1‑149 (MQ=255)                                                                                                       tCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCaccacc                                        >  4:17246/1‑149 (MQ=255)                                                                                                          aTAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCa                                     >  4:196072/1‑149 (MQ=255)                                                                                                           tAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCAc                                    >  3:303483/1‑149 (MQ=255)                                                                                                            aaCCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCAcc                                   >  2:286334/1‑149 (MQ=255)                                                                                                                gCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTc                               <  3:395769/149‑1 (MQ=255)                                                                                                                       cATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCATCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACcg                        >  4:30559/1‑149 (MQ=255)                                                                                                                        aTGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCAccc                                  <  2:580730/138‑1 (MQ=255)                                                                                                                        aTGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCAccc                                  >  1:580730/1‑138 (MQ=255)                                                                                                                                ggTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCa               >  3:272867/1‑149 (MQ=255)                                                                                                                                       acCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTc        >  3:270792/1‑149 (MQ=255)                                                                                                                                             atcaGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTGACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCa  <  2:168579/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 AGGCAGCATGGCTGACGAGAGTAAAATCGACGGCTTTTCCCAGCCCTAAAACGTTGGTCATCACGCCATACAGCAGACAGGCGACAATTAATCCTACGAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCA  >  NC_000913/1069143‑1069431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑