Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,177,156 A→G 6.7% N180D (AAC→GAC)  lolD → outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,177,1560AG 6.7% 190.7 / 3.9 60N180D (AAC→GAC) lolDouter membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base G (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

GTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCC  >  NC_000913/1177015‑1177303
                                                                                                                                             |                                                                                                                                                   
gTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGa                                                                                                                                              <  2:1834/149‑1 (MQ=255)
    ggCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGc                                                                                                                                          >  1:133806/1‑149 (MQ=255)
     gCTGGATCATCGTGCGACTCACCGCCCATCTGCACTTTCTGGCGGCGACCGCCAGCGTGTGGCGATTGCCCCTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTAAAGGTAACCTCGATGCGCGTATCGCCGACAGCa                                                                                                                                         >  2:428426/1‑149 (MQ=255)
     gCTGGATCATCGTGCGAATCGGCGCCTTGCTGAACGTTCTGGCGGCGAACGCCAGCGTGTGGCGTTTGCCGCTGCGCTGGCGTGTACATCGATCCTGGTACTGGCGGATGAACCTACCGGTGACCTCGATGCGCGTAACGCCGACAGcc                                                                                                                                         <  2:536083/149‑2 (MQ=255)
     gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGGATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCa                                                                                                                                         >  4:55503/1‑149 (MQ=255)
     gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCa                                                                                                                                         >  3:16265/1‑149 (MQ=255)
       tGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATc                                                                                                                                       >  2:128097/1‑149 (MQ=255)
        ggATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCt                                                                                                                                      <  1:128097/149‑1 (MQ=255)
          atcatcGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTc                                                                                                                                    <  2:173089/149‑1 (MQ=255)
           tcatcGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTcc                                                                                                                                   >  1:173707/1‑149 (MQ=255)
                gTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTg                                                                                                                              >  3:469281/1‑149 (MQ=255)
                         aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGaa                                                                                                                     >  2:358396/1‑149 (MQ=255)
                          ccGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAAt                                                                                                                    <  4:566506/149‑1 (MQ=255)
                           cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAAtt                                                                                                                   <  1:541308/149‑1 (MQ=255)
                           cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAAtt                                                                                                                   <  1:117807/149‑1 (MQ=255)
                           cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAAtt                                                                                                                   <  4:370098/149‑1 (MQ=255)
                           cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAAtt                                                                                                                   <  1:486087/149‑1 (MQ=255)
                              ccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGaa                                                                                                                >  1:565659/1‑149 (MQ=255)
                               cATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGtt                                                                                                                               >  4:288604/1‑133 (MQ=255)
                               cATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGtt                                                                                                                               <  3:288604/133‑1 (MQ=255)
                                 tCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCg                                                                                                             >  3:191029/1‑149 (MQ=255)
                                 tCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCg                                                                                                             >  2:21192/1‑149 (MQ=255)
                                 tCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGGTGCTTGGGGAATTGAATCg                                                                                                             >  1:30845/1‑149 (MQ=255)
                                  cTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGc                                                                                                            >  1:475243/1‑149 (MQ=255)
                                       cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCa                                                                                                       <  2:541507/149‑1 (MQ=255)
                                        tttCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAg                                                                                                      >  2:113466/1‑149 (MQ=255)
                                                                  gCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAAGCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCa                                                                            >  2:500586/1‑149 (MQ=255)
                                                                     aTTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGa                                                                         >  3:20917/1‑149 (MQ=255)
                                                                     aTTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGa                                                                         <  2:62805/149‑1 (MQ=255)
                                                                        gCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCt                                                                      <  2:132837/149‑1 (MQ=255)
                                                                            gTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCaa                                                                  >  4:389823/1‑149 (MQ=255)
                                                                               cgcTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTg                                                               <  2:341235/149‑1 (MQ=255)
                                                                                  tGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGa                                                                         <  1:332744/136‑1 (MQ=255)
                                                                                  tGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGa                                                                         >  2:332744/1‑136 (MQ=255)
                                                                                  tGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCg                                                            >  1:281776/1‑149 (MQ=255)
                                                                                         tAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTa                                                     <  4:191029/149‑1 (MQ=255)
                                                                                         tAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTa                                                     >  1:251575/1‑149 (MQ=255)
                                                                                            cccGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGa                                                  <  4:20917/149‑1 (MQ=255)
                                                                                            cccGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGa                                                  <  3:426634/149‑1 (MQ=255)
                                                                                                    tGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAg                                          <  3:531941/149‑1 (MQ=255)
                                                                                                     ggTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGt                                         <  2:227082/149‑1 (MQ=255)
                                                                                                       tACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTa                                       >  2:322311/1‑149 (MQ=255)
                                                                                                              ggATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGc                                <  2:565659/149‑1 (MQ=255)
                                                                                                                 tGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTg                             >  3:390562/1‑149 (MQ=255)
                                                                                                                  gAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGGGCCGCCAGTTAGAAATGCGTGa                            >  1:224353/1‑149 (MQ=255)
                                                                                                                  gAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGa                            <  3:389823/149‑1 (MQ=255)
                                                                                                                    aCCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATg                          <  1:500586/149‑1 (MQ=255)
                                                                                                                    aCCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATg                          <  3:440210/149‑1 (MQ=255)
                                                                                                                      cTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgt                        >  1:584502/1‑149 (MQ=255)
                                                                                                                      cTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgt                        >  4:17306/1‑149 (MQ=255)
                                                                                                                             tAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGa                 >  2:435440/1‑149 (MQ=255)
                                                                                                                              aaCCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGAc                <  1:322311/149‑1 (MQ=255)
                                                                                                                                ccTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGAcgg              >  3:198409/1‑149 (MQ=255)
                                                                                                                                     aTGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGaa         <  1:21192/149‑1 (MQ=255)
                                                                                                                                      tGCGCGTGACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGaa                                    <  4:142547/121‑1 (MQ=255)
                                                                                                                                      tGCGCGTGACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGaa                                    <  2:80991/121‑1 (MQ=255)
                                                                                                                                      tGCGCGTGACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGaa                                    >  3:142547/1‑121 (MQ=255)
                                                                                                                                      tGCGCGTGACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGaa                                    >  1:80991/1‑121 (MQ=255)
                                                                                                                                           gTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGc   <  4:390562/149‑1 (MQ=255)
                                                                                                                                            tAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGcc  <  4:469281/149‑1 (MQ=255)
                                                                                                                                             aaCGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGc                                >  3:560656/1‑118 (MQ=255)
                                                                                                                                             aaCGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGc                                <  4:560656/118‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCC  >  NC_000913/1177015‑1177303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑