Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,444,587 G→A 6.1% G513R (GGG→AGG)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,444,5870GA 6.1% 224.2 / 3.0 69G513R (GGG→AGG) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (40/24);  new base A (2/2);  total (43/26)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

ACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGG  >  NC_000913/1444440‑1444735
                                                                                                                                                   |                                                                                                                                                    
aCGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCgg                                                                                                                                                     <  4:516237/149‑1 (MQ=255)
        cGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGTGCAAACGc                                                                                                                                             >  1:180178/1‑149 (MQ=255)
        cGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGc                                                                                                                                             >  4:174628/1‑149 (MQ=255)
        cGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGc                                                                                                                                             >  1:234462/1‑149 (MQ=255)
        cGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGc                                                                                                                                             >  3:61893/1‑149 (MQ=255)
         gATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCt                                                                                                                                            >  3:97118/1‑149 (MQ=255)
          aTCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTg                                                                                                                                           >  1:532025/1‑149 (MQ=255)
          aTCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTg                                                                                                                                           >  4:163657/1‑149 (MQ=255)
             tctcCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTc                                                                                                                                        >  2:582339/1‑149 (MQ=255)
             tctcCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTc                                                                                                                                        >  1:419979/1‑149 (MQ=255)
               tcCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCAGTGGACGCCGGGCAAACGCTGTTCAc                                                                                                                                      >  3:548790/1‑149 (MQ=255)
                ccACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCAGGCAAACGCTGTTCACt                                                                                                                                     >  2:172360/1‑149 (MQ=255)
                ccACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCAGGCAAACGCTGTTCACt                                                                                                                                     >  4:397825/1‑149 (MQ=255)
                 cACCGGTTTCGACCAGTTACAATACGGTATGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTg                                                                                                                                    >  2:175636/1‑149 (MQ=255)
                   ccGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTggc                                                                                                                                  <  4:521369/149‑1 (MQ=255)
                      gTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTggcggc                                                                                                                               >  3:458536/1‑149 (MQ=255)
                       tttCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCa                                                                                                                              <  2:537852/149‑1 (MQ=255)
                         tCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAgt                                                                                                                            <  2:532025/149‑1 (MQ=255)
                           gACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAgtgt                                                                                                                          >  1:425006/1‑149 (MQ=255)
                           gACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAgtgt                                                                                                                          <  2:578359/149‑1 (MQ=255)
                               aGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTa                                                                                                                      >  1:161032/1‑149 (MQ=255)
                                gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTAc                                                                                                                     >  1:144194/1‑149 (MQ=255)
                                          ggTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAAc                                                                                                           <  1:219963/149‑1 (MQ=255)
                                                     ggTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTa                                                                                                                      >  3:335984/1‑127 (MQ=255)
                                                     ggTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTa                                                                                                                      <  4:335984/127‑1 (MQ=255)
                                                       tAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGc                                                                                              <  1:457319/149‑1 (MQ=255)
                                                          aaaaGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTc                                                                                           <  3:10348/149‑1 (MQ=255)
                                                              gCCGCGATTAATTCTCGACAACCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATg                                                                                       >  1:342001/1‑149 (MQ=255)
                                                               ccGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGTGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATgg                                                                                      <  2:180178/149‑1 (MQ=255)
                                                               ccGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGCGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATgg                                                                                      >  4:380785/1‑149 (MQ=255)
                                                                         ttCTCGGCAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATcct                                                                            >  2:413598/1‑149 (MQ=255)
                                                                         ttCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATcct                                                                            >  4:387522/1‑149 (MQ=255)
                                                                         ttCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCGGTCACTGGACGCCCGGCAAACGCTGTTCACTGGCTGCAGTGTGTTACCACCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATcct                                                                            >  1:347351/1‑149 (MQ=255)
                                                                          tctcGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATccta                                                                           >  1:96130/1‑149 (MQ=255)
                                                                                     ccATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCctc                                                                <  1:15019/149‑1 (MQ=255)
                                                                                     ccATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCctc                                                                <  2:25184/149‑1 (MQ=255)
                                                                                      cATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCctct                                                               >  1:142216/1‑149 (MQ=255)
                                                                                       aTCGCCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCtt                                                              >  2:388293/1‑149 (MQ=255)
                                                                                           tCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTaaa                                                          <  4:97118/149‑1 (MQ=255)
                                                                                                  aCGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGAtt                                                   >  4:454831/1‑149 (MQ=255)
                                                                                                    gTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTa                                                 <  2:267397/149‑1 (MQ=255)
                                                                                                    gTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTa                                                 <  2:363645/149‑1 (MQ=255)
                                                                                                     tGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTAc                                                >  2:307988/1‑149 (MQ=255)
                                                                                                         gCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGc                                            >  1:328385/1‑149 (MQ=255)
                                                                                                              gCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTg                                       <  3:174628/149‑1 (MQ=255)
                                                                                                               cATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTga                                      <  2:136428/149‑1 (MQ=255)
                                                                                                                aTCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTgag                                     >  3:38707/1‑149 (MQ=255)
                                                                                                                  cccTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGAt                                   >  4:378676/1‑149 (MQ=255)
                                                                                                                   cctcctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGAtt                                  >  1:243404/1‑149 (MQ=255)
                                                                                                                   cctcctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGAtt                                  >  3:139733/1‑149 (MQ=255)
                                                                                                                   cctcctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCAGCAACCTTAAAATGTAGCGTCGCTGGGCGCGGTCCTACCTATTTCCTCTTGAAAGGCGATTTACATGCTGGTGAGAtt                                  >  4:460447/1‑149 (MQ=255)
                                                                                                                   cctcctTTAGCGGCGCGCTGTCACTGGACGCCAGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGAtt                                  <  3:397825/149‑1 (MQ=255)
                                                                                                                   cctcctTTAGCGGCGCGCTGTCACTGGACGCCAGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGAtt                                  <  1:172360/149‑1 (MQ=255)
                                                                                                                       ctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGcc                              >  3:389613/1‑149 (MQ=255)
                                                                                                                        tttAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGccc                             >  1:67754/1‑149 (MQ=255)
                                                                                                                            gcggcgCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGtt                         >  1:49744/1‑149 (MQ=255)
                                                                                                                              ggcgCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCg                       >  1:430411/1‑149 (MQ=255)
                                                                                                                               gcgcgcTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCgg                      <  3:46512/149‑1 (MQ=255)
                                                                                                                               gcgcgcTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTGAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAAGCGATTTACATGCTGGTGAGATTGGCCCGGTTCgg                      <  3:515046/149‑1 (MQ=255)
                                                                                                                                    cTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTaaa                 <  2:419979/149‑1 (MQ=255)
                                                                                                                                        cACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGt             <  3:163657/149‑1 (MQ=255)
                                                                                                                                         aCTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTc            <  3:378676/149‑1 (MQ=255)
                                                                                                                                         aCTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTc            <  2:425006/149‑1 (MQ=255)
                                                                                                                                           tGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGc          >  1:281981/1‑149 (MQ=255)
                                                                                                                                           tGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGc          >  1:574515/1‑149 (MQ=255)
                                                                                                                                            ggACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCt         >  2:41417/1‑149 (MQ=255)
                                                                                                                                             gACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTg        >  3:213355/1‑149 (MQ=255)
                                                                                                                                                gCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGa     <  4:420729/149‑1 (MQ=255)
                                                                                                                                                gCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGa     >  4:535739/1‑149 (MQ=255)
                                                                                                                                                 ccGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATCTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGAc    <  4:326513/149‑1 (MQ=255)
                                                                                                                                                   gggCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACgg  <  4:458536/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGG  >  NC_000913/1444440‑1444735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑