Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,539,982 C→T 5.9% R870H (CGC→CAC)  narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,539,9820CT 5.9% 224.4 / 3.3 68R870H (CGC→CAC) narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (38/26);  new base T (2/2);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

CACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCT  >  NC_000913/1539838‑1540130
                                                                                                                                                |                                                                                                                                                    
cACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCa                                                                                                                                                  >  1:505772/1‑149 (MQ=255)
  cGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGt                                                                                                                                                <  1:324344/149‑1 (MQ=255)
        cccTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCa                                                                                                                                          >  4:223594/1‑149 (MQ=255)
         ccTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCAc                                                                                                                                         >  1:486043/1‑149 (MQ=255)
           tttACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACtt                                                                                                                                       >  2:162313/1‑149 (MQ=255)
                cGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCacac                                                                                                                                  >  2:24783/1‑149 (MQ=255)
                  ccATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAACGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACgg                                                                                                                                >  1:550036/1‑149 (MQ=255)
                       gCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCtgtg                                                                                                                           >  1:254470/1‑149 (MQ=255)
                         cAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTgag                                                                                                                         >  3:180758/1‑149 (MQ=255)
                          aaGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTgaga                                                                                                                        >  3:343228/1‑149 (MQ=255)
                          aaGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTgaga                                                                                                                        >  1:94546/1‑149 (MQ=255)
                           aGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGagag                                                                                                                       <  4:431278/149‑1 (MQ=255)
                            gTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGc                                                                                                                      >  1:107031/1‑149 (MQ=255)
                              ttGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGctc                                                                                                                    <  1:489461/149‑1 (MQ=255)
                                 tCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGc                                                                                                                 >  4:304688/1‑149 (MQ=255)
                                 tCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGc                                                                                                                 >  1:486953/1‑149 (MQ=255)
                                   cATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCg                                                                                                               >  1:74533/1‑149 (MQ=255)
                                   cATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCg                                                                                                               >  1:111138/1‑149 (MQ=255)
                                    aTCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGTGCCAGTc                                                                                                                                               <  1:482369/116‑1 (MQ=255)
                                    aTCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGTGCCAGTc                                                                                                                                               <  3:422842/116‑1 (MQ=255)
                                    aTCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGTGCCAGTc                                                                                                                                               >  2:482369/1‑116 (MQ=255)
                                    aTCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGTGCCAGTc                                                                                                                                               >  4:422842/1‑116 (MQ=255)
                                    aTCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCgg                                                                                                              >  3:196269/1‑149 (MQ=255)
                                     tCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGga                                                                                                             >  2:518616/1‑149 (MQ=255)
                                       aaTGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGgaga                                                                                                           <  1:362473/149‑1 (MQ=255)
                                       aaTGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGgaga                                                                                                           >  2:222965/1‑149 (MQ=255)
                                         tGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGt                                                                                                         >  2:208459/1‑149 (MQ=255)
                                          ggCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTc                                                                                                        <  2:581894/149‑1 (MQ=255)
                                            cccGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCAt                                                                                                      >  3:110758/1‑149 (MQ=255)
                                             ccGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATg                                                                                                     >  2:48811/1‑149 (MQ=255)
                                                agcgagGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAg                                                                                                  <  4:110758/149‑1 (MQ=255)
                                                   gagGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAg                                                                                               <  3:439066/149‑1 (MQ=255)
                                                    agGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAgg                                                                                              <  2:486953/149‑1 (MQ=255)
                                                     ggTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAggg                                                                                             <  1:569998/149‑1 (MQ=255)
                                                       tAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGt                                                                                           >  2:139362/1‑149 (MQ=255)
                                                         aaGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTg                                                                                         >  1:340351/1‑149 (MQ=255)
                                                          aGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGt                                                                                        >  1:433191/1‑149 (MQ=255)
                                                           gCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTa                                                                                       >  1:4407/1‑149 (MQ=255)
                                                                  tACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGACACCTTTGCCGGAGAGTTATGCAGTAGGGATTGTATTCCCAc                                                                                <  2:259912/149‑1 (MQ=255)
                                                                      tAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCg                                                                            <  1:184048/149‑1 (MQ=255)
                                                                      tAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCg                                                                            <  1:162313/149‑1 (MQ=255)
                                                                        gCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGga                                                                                                             >  4:63896/1‑114 (MQ=255)
                                                                        gCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGga                                                                                                             <  3:63896/114‑1 (MQ=255)
                                                                              tAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCttt                                                                    >  2:43200/1‑149 (MQ=255)
                                                                                 tCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGcc                                                                 >  4:54454/1‑149 (MQ=255)
                                                                                   gcgcTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACGACGTCGGTTTCTTTGCCaa                                                               >  1:118399/1‑149 (MQ=255)
                                                                                   gcgcTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCaa                                                               >  1:350679/1‑149 (MQ=255)
                                                                                         cACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAc                                                         >  1:522237/1‑149 (MQ=255)
                                                                                           ccGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAccc                                                       >  4:95984/1‑149 (MQ=255)
                                                                                           ccGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGAccc                                                       <  3:285574/149‑1 (MQ=255)
                                                                                               cacaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGc                                                   >  3:239199/1‑149 (MQ=255)
                                                                                                acaATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGca                                                  >  4:213830/1‑149 (MQ=255)
                                                                                                       tCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGc                                           <  3:213830/149‑1 (MQ=255)
                                                                                                             cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAAt                                     <  2:94546/149‑1 (MQ=255)
                                                                                                              ggTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATg                                    <  2:111138/149‑1 (MQ=255)
                                                                                                              ggTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATg                                    <  2:74533/149‑1 (MQ=255)
                                                                                                                 tttGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCtt                                 <  4:343228/149‑1 (MQ=255)
                                                                                                                    gCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTgg                              <  3:462267/149‑1 (MQ=255)
                                                                                                                        gtaaTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCGCTGCCGGAGAGTCATGCAGTAGGGGTTTTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGAt                          <  2:168359/147‑1 (MQ=255)
                                                                                                                           aTCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATAcc                       >  2:464221/1‑149 (MQ=255)
                                                                                                                            tCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCt                      <  1:518616/149‑1 (MQ=255)
                                                                                                                               gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTg                   >  2:370696/1‑149 (MQ=255)
                                                                                                                               gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTg                   >  4:236453/1‑149 (MQ=255)
                                                                                                                               gATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTg                   >  3:514286/1‑149 (MQ=255)
                                                                                                                                 tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACCCGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTCGGGGTTGTAATACAACGTCGGTTTCTTTACCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACTTTTGTa                 <  4:545827/149‑1 (MQ=255)
                                                                                                                                    cATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGAt              <  3:21238/149‑1 (MQ=255)
                                                                                                                                     aTTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGAtt             <  2:433191/149‑1 (MQ=255)
                                                                                                                                             ttGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTc     <  2:4407/149‑1 (MQ=255)
                                                                                                                                                cgcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCt  >  4:297487/1‑149 (MQ=255)
                                                                                                                                                cgcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGATTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCt  >  4:371797/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CACGAAATCCCTTTACCGCCATTGCCAAGTTTGTCCATCAATGGCCCGAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCT  >  NC_000913/1539838‑1540130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑