Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,3130CA98.3% 804.9 / NA 235S683Y (TCC→TAC) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (127/103);  total (131/104)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145042‑2145584
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                               
   gaagCGTTAGGGGTTCCGCTCCTGAGTGTGTTACATATTAATTTTGGCGGCAAAGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCatg                                                                                                                                                                                                                                                                            <  1:634902/274‑1 (MQ=255)
               gtTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGc                                                                                                                                                                                                                                                                <  1:550651/274‑1 (MQ=255)
                 tCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCCCGc                                                                                                                                                                                                                                                         >  2:642302/1‑279 (MQ=255)
                  ccGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCAtct                                                                                                                                                                                                                                                            >  1:28997/1‑275 (MQ=255)
                  ccGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGGTACCGAATCACGCCAAATGCTGCGCCCGCTGAGCTACCGCGCCTACCATTATTCCCTGACggctc                                                                                                                                                                                                                                                             >  3:296867/1‑270 (MQ=255)
                        cTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACcgcg                                                                                                                                                                                                                                                 >  2:193318/1‑280 (MQ=255)
                          gACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCgcgc                                                                                                                                                                                                                                                <  2:229053/279‑1 (MQ=255)
                          gACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGcc                                                                                                                                                                                                                                               >  2:606089/1‑280 (MQ=255)
                              gggtTGCATATTACTGTGGGGAACAACGGACCATTAATGGAGAAGAGTTACAGTGCCGCAACCTCAGGTGCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTGAGTACTCTGGACGGCAGAGATATTGGTTCGGTTGTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCgcgc                                                                                                                                                                                                                                                <  3:669133/273‑1 (MQ=255)
                                    cATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCAGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGAAGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGCATCACGCAAAATGCTCCGCCAGCTGAGCTACAGCGCCTAACATGATGCACTGACGCATCTCGCCAACCACGCCAGTTTTgagg                                                                                                                                                                                                                                     >  2:664969/1‑279 (MQ=255)
                                     atatTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTgaga                                                                                                                                                                                                                                     <  2:723899/279‑1 (MQ=255)
                                         tACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCCGCGCCCGCTGCACTACCGCTCCTCCCCTGATGCACCTACGCATCCCGCCACCCGCCCCAGCCTTTAGAaaca                                                                                                                                                                                                                                 >  4:716680/1‑279 (MQ=255)
                                              ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAACATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGCAACAACTGc                                                                                                                                                                                                                            >  2:912994/1‑279 (MQ=255)
                                              ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGTTGAGCTACATGGCCTACCATGATGCCCTGACGCATCTCGCCCACCGCCCCAGTTTTGCGAaacaa                                                                                                                                                                                                                                >  3:1195112/1‑275 (MQ=255)
                                              ttGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAaaca                                                                                                                                                                                                                                 >  1:797388/1‑274 (MQ=255)
                                                                tgaTGGATAACAATTACCGTGCCGACACCGCACGTTCCGCGATAGAGCAAGATGTGGTGGTGAACTGTCGGAGCGGAGGCATTTACGACGCTCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCCATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGt                                                                                                                                                                                                         <  2:423836/278‑1 (MQ=255)
                                                                       gaACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCCCCTACCATTATGCACTGACGCCTCCCCCCAACCCCCCCAGTTTTGAGCAACACACGCGTAACCTGCTCCacaacgtacaccg                                                                                                                                                                                                   >  4:437216/1‑267 (MQ=255)
                                                                         aCATTTACAGGGCCGACACCTCAAGTTCCGCGGTCGGGCAAGATGGGGTGTTGCACTGGCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGAAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                      <  1:588403/274‑1 (MQ=255)
                                                                             ttACGGTGCCGAAGCCTAACGGTCCGGGATCGGGCAAGATGTGTGGTGGCACTTGCGGAGCGGCTGCAGTTAAGACGTGCATTACAGTATTACGCCGTGAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                 <  3:522493/275‑1 (MQ=255)
                                                                             ttACAGGGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTa                                                                                                                                                                                                 <  1:144288/275‑1 (MQ=255)
                                                                                   tGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACGGCGTATCCTGCTGCAAACGGTAAACAGTAACCATc                                                                                                                                                                                           >  1:1169168/1‑275 (MQ=255)
                                                                                     ccGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGACTCACGCAAAATGCTGCGCCAGCTGAGCTACAGGGCCTACCATGATGCACTGACGCATCTCGCCACCCGCACCAGTTTTGATAACCACCTCCTTAACCTGCTGCCACCCGTAAACAGtccccacacacgca                                                                                                                                                                                     >  4:478970/1‑266 (MQ=255)
                                                                                         cacCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGAc                                                                                                                                                                                     <  3:142054/275‑1 (MQ=255)
                                                                                          acCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa                                                                                                                                                                                    <  1:900697/275‑1 (MQ=255)
                                                                                                cGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGgtgt                                                                                                                                                                         <  2:617596/280‑1 (MQ=255)
                                                                                                  ttCCGCGAGCGAGGAAGATGTGGGTGTGCCCTGGCGGTGCGGCGGCAGTTACGACGTGCATTAAGGTATTACGCCGTTAAGTACTCTTGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTgg                                                                                                                                                                            <  3:380720/275‑1 (MQ=255)
                                                                                                      gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCg                                                                                                                                                                   >  2:324512/1‑280 (MQ=255)
                                                                                                      gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGCTTTGGGAAACAACTCCGTATCCTCCTGCAAACGGTAAACAGTACACATACGCGACATGCCCTGgggt                                                                                                                                                                         >  3:399530/1‑274 (MQ=255)
                                                                                                      gcgATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACCACGTGCATTACAGTATTACGCCGTTAAGTACTCCGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCTGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTCGATCTACAACTGCGTATCCTGCTTCAAACGGTAAACCGTACACATCAGCCACATccccg                                                                                                                                                                              >  3:27082/1‑268 (MQ=255)
                                                                                                              tgaaGTTGTGGTGTTGGACTGGGGGAGCGGCGGCAGTTACGACGGGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                           <  4:175953/278‑1 (MQ=255)
                                                                                                              gCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGAt                                                                                                                                                           <  2:793214/280‑1 (MQ=255)
                                                                                                                  gATGTTGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTAGGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGa                                                                                                                                                            <  1:42078/275‑1 (MQ=255)
                                                                                                                         ggtttgaCTGGCGGAGCGGCGGCAGTTCGGACGTGGATTAAAGTTTTACGCCGTTAAGTACTCTGGACGGAAGCAATATTGGGTCGGTTCTGGTGATTCAGGACGTTACCGAATCCCGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                 <  4:912994/273‑1 (MQ=255)
                                                                                                                             gCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTAAGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTCGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACAACAGCGACATGACCTGGTGTTTATCGATCTTGATCGCTTTAAAGAGGTg                                                                                                                                            >  2:70499/1‑280 (MQ=255)
                                                                                                                              cACTGTCGGGGCGGCGTCAGTTACGACGTGCATTACAGTAGTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCCGCTGCAAACGGTAAACAGTACACATCAGCGACATGCTCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                 <  1:856755/274‑1 (MQ=255)
                                                                                                                              cACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTac                                                                                                                                                                                                >  2:826015/1‑227 (MQ=255)
                                                                                                                               acggTCGGAGGGGCGGCAGTTACGACGTGCATTAAAGTATTACGCCGTTAAGTACTCTGGACGGCAGAAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCg                                                                                                                                               <  3:1169168/272‑1 (MQ=255)
                                                                                                                                  gTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATAAGCGACATGCCCATGTGTTTATCGATCTTGATCTCTTTAAAGCGGTGAATGa                                                                                                                                       >  4:63515/1‑279 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGCGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGCCATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAAAGCGGTTAATGa                                                                                                                                       >  2:679336/1‑279 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCCAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTg                                                                                                                                            >  1:347633/1‑274 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACCCATCAGCGAAATGCCCTGGTGTTTATCGATCTTTATCGCTTTAAAGCGGTg                                                                                                                                            >  1:1154317/1‑274 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCgtgt                                                                                                                                            >  3:1129548/1‑271 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTg                                                                                                                                            >  1:224481/1‑274 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCACCTGAGCGACAGCGCCTACCCTGATGCACTGACGCATCTCGCCAACCGCGCCACGTTTGCTAACCAACTGCGTAACCTGCTCCACACGGTTAACTATACACACCAGACACATGCCCTGGCGTTTATCGACCTTGATCGCTTTAAATCgcta                                                                                                                                            >  3:186158/1‑271 (MQ=255)
                                                                                                                                   tCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGATGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCTTCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTCTCCTGATGCAAACGGTAAACAGTACACGTCAGCGCCATGCACTGCTTTTTATCCATCTTGCGCTCTTTAAAGGGTTGAATGaa                                                                                                                                      >  4:762966/1‑279 (MQ=255)
                                                                                                                                             gggaGTTACGGAGGGCAGTACAATATTACGCCGTTAAGTACTCTGGACGGCAGCAATATGGGTTGGGTTCTGTTGATTCAGTGCGTTGCCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAGCGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAgcgc                                                                                                                                 <  3:931846/272‑1 (MQ=255)
                                                                                                                                             ggcAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAgcgc                                                                                                                                 <  1:1195112/275‑1 (MQ=255)
                                                                                                                                                      gacgTGCATTACAGTAGTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATg                                                                                                                         <  1:110849/274‑1 (MQ=255)
                                                                                                                                                                    tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACCGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGATAAACAACTGCGTATCCTTCTGCAAACGGTAAACAGTACACATCAGCGACCTGCCCTGGTGTTTATCGCCCTTGTGCGCTATAAAGCGGGTAATGACAGAGCCGGGAATGCGGCGGGGGGCGCtt                                                                                                          >  3:440264/1‑275 (MQ=255)
                                                                                                                                                                    tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACa                                                                                                                                                                                                    <  2:184866/185‑1 (MQ=255)
                                                                                                                                                                    tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGAAAGCGCCGGGCATGCGGCGGGTGACGCTTtgctg                                                                                                     >  2:249140/1‑280 (MQ=255)
                                                                                                                                                                    tATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGGGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGGTCCGGTCATGCGGCGGGTGACGCtt                                                                                                          >  1:507216/1‑275 (MQ=255)
                                                                                                                                                                       tACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGCTCGCTTTAACTCGGTGCATGACAGCGCCGGGCCTGCGCCGGGTTGCGCTTtgctgctc                                                                                                  >  4:54867/1‑278 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTTGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGATGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATTCTGCTGCAAACGGTAAACAGTACCCATCTGCGACATGCCCGGGTGTTTAAAGATCTTGATCGCTTTAAAGTGGTGAATTACCGCCCCGGGCATGCGGCGGGTAACGGTTtgctg                                                                                                     >  3:772582/1‑275 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCCCTGACGCATCTCGCCAACCGCGCCAGTTTTGAGCAACACCTGCGTATCCTGCTGCAACCGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATTGCTTTAAAGCGGTGAAGGAAAGACCCCGGCATGCGGCGGGTGACGCTTtg                                                                                                        >  3:948990/1‑272 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCggg                                                                                                                             >  2:68394/1‑251 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGCCGCTTtgctg                                                                                                     >  1:159468/1‑275 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                >  2:17333/1‑280 (MQ=255)
                                                                                                                                                                         cGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGa                                                                                                >  4:40224/1‑280 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTTAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACAAATCAGCGACATGCCCTGGTGTTTCACACTCTTGATCGTTTTAAATCGGTGAATGACAGCGCCGGGCATGCTGCGGTTGAGCGTTtgctttcggcactgc                                                                                           >  4:310530/1‑269 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTTAGAAACAACTGCGTATCCTGCTGCAAACGGTACACAGTACACATCAGCGCCATGCCCTGGTGTTTATCGCTCTTTATCGCTTTAAAGCGGTGAATGACAGCGCCGGGACTGCGCCGGGTGTCGCTTTCCTTCGCGa                                                                                                >  3:990808/1‑275 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTa                                                                                                                                                                                                        >  4:681165/1‑171 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTCAACATTACACCTCAGCGACATCCCCCGGTTGTTCTCGATCTTGCTCTCTTTACAGCGGTGAATGTCAGCGCCGGGCATTCGGCGGGTTGCGCTTCGCTGCCGCAACTgg                                                                                           >  4:270509/1‑280 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGCACggg                                                                                           >  2:224578/1‑280 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAACGGGTGAATGACAGCGCCGGGCATGCGGCGGGTGAGGCTTTGCTGCTCGa                                                                                                >  3:1090044/1‑275 (MQ=255)
                                                                                                                                                                              ttAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCCCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATCCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTTAATGACCGCCACTGGCATGGGGCGGTGGACGCTTTGCCGCGGGAACt                                                                                             >  4:901179/1‑278 (MQ=255)
                                                                                                                                                                               tAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  2:451480/280‑1 (MQ=255)
                                                                                                                                                                                 aGTACGCGGGACGGCAGCAATATTGGTTCGGTGCGGGTGATTCAGGACGTGACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCGGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCTGGCATGCGGCGGGTGACGCTTTGCTTCGCGAACTGGc                                                                                          <  2:54867/278‑1 (MQ=255)
                                                                                                                                                                                    aCTCTTGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  1:298001/275‑1 (MQ=255)
                                                                                                                                                                                     ctctGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGAAGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGc                                                                                          <  1:772582/274‑1 (MQ=255)
                                                                                                                                                                                        tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTaaa                                                                                                                                                                                                      >  4:326502/1‑163 (MQ=255)
                                                                                                                                                                                        tGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCttt                                                                                                                                                     >  1:1060892/1‑212 (MQ=255)
                                                                                                                                                                                              gaagcaATATTGGTTCGGTGCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGGGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATg                                                                                <  1:39179/273‑1 (MQ=255)
                                                                                                                                                                                               cagcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCttt                                                                                                                                                     >  1:317995/1‑205 (MQ=255)
                                                                                                                                                                                                agcaATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGt                                                                          >  2:498282/1‑279 (MQ=255)
                                                                                                                                                                                                  caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                           <  2:853662/148‑1 (MQ=255)
                                                                                                                                                                                                  caATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACg                                                                                                                                                                                                           >  4:853662/1‑148 (MQ=255)
                                                                                                                                                                                                  aaaTATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACAGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCt                                                                              <  1:1207809/272‑1 (MQ=255)
                                                                                                                                                                                                   aaTATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAg                                                                                                                                                 <  3:317995/205‑1 (MQ=255)
                                                                                                                                                                                                            tcggttcTGGTGATTCGGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACa                                                                                                                                                                                    >  3:68224/1‑161 (MQ=255)
                                                                                                                                                                                                             cggttcTGGGGAGTCAGGACGTTACCGAAACACGAAGAGTGCTGCGCCAGCTGAGCTACAACGCCTACCAGGATGCACTGGCGCAACTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATGCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAg                                                            <  4:189609/280‑1 (MQ=255)
                                                                                                                                                                                                                     gTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGGGTTTATCGATCTTGATCGCTTTAAAGCGATGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGACATGGCGTCGTTAATGATGAGTATGCGGAGTTCCAGCGACGTGc                                                    >  2:339536/1‑280 (MQ=255)
                                                                                                                                                                                                                              ggcGTTAACGAATCACGACGAATGCTGTGCCCGCTGAGCTCCGGCGCCTACCATTATGGACTGAGGCACCTCGCCAACCGAGCCAGTTTTGAGAAACAAATGCGTATCCTGCTGCAAACGGTAAAAAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGa                                            <  4:644989/277‑1 (MQ=255)
                                                                                                                                                                                                                              gACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGctggctctttt                                                 >  1:671162/1‑264 (MQ=255)
                                                                                                                                                                                                                                 gTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTAtgctg                                                                    <  2:377683/252‑1 (MQ=255)
                                                                                                                                                                                                                                  taaCCGAATCACGCAAAATGCTGCGCCAACTGCGCAACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGa                                            <  3:797388/273‑1 (MQ=255)
                                                                                                                                                                                                                                          tCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTTCTGGCGCGACTCGGt                                      >  3:1027707/1‑273 (MQ=255)
                                                                                                                                                                                                                                             cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGCCAGCGCCCGGCATGCGGCGGGTGACGCTTTGCTGCGCGCACTGGCGCCGTGAATGCTGAGTATGCTGCGTTCCAGCGACGGGCTGGCCCGACTCGGGGGCGCTGAAt                            >  4:141530/1‑280 (MQ=255)
                                                                                                                                                                                                                                             cGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTggg                                   >  1:1054666/1‑273 (MQ=255)
                                                                                                                                                                                                                                                      ctgcGCCAGCTGAGCTACAGCGCCTACCATGATGCCCGGACGCATCTCGCCAACCGGGCCAGTTTTGGGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  2:739506/280‑1 (MQ=255)
                                                                                                                                                                                                                                                      atgcGCCAGCTGAGCCACAGCGCCAACCATACTGCACTGACGCATCTCGCCAACCGCGCCCGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTctgc                   <  4:214733/279‑1 (MQ=255)
                                                                                                                                                                                                                                                       tgcGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGAc                                           >  1:1153122/1‑255 (MQ=255)
                                                                                                                                                                                                                                                        gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTTTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGAGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAGAGCTGAGTATGCTGCGTCCCAGCGACGTGCTGGCGCGACTCGTTGCGGACGACTTCggct                      >  3:1155648/1‑273 (MQ=255)
                                                                                                                                                                                                                                                        gcgcCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTTCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGCGGTGATGAATTTgg                        >  1:1026751/1‑273 (MQ=255)
                                                                                                                                                                                                                                                              gCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa           <  2:63515/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTAc             <  1:939552/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gCTCCAGCGCCTACCAGGATGCACTGACGCATCGCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGGAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa           <  3:554231/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                   gCTACCGCGCCTACACGGATGCATTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACGGCGTATCCTGCTGACAACGGTAAACAGTACACATCAGCGACATGCCCTTGTGTTTATCGATCTTGATCGCTGTAAAGCGGTGAATGACAGCGCCGGGCAGGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCa           <  3:1148894/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cTACAGCGCCTACCATGATGCACTCACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTACAGCGGTGAATTACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTTCTCCACTTGGCTGCGTTCATGCTTGGTATGCTGCGTTCCAGCGATGTCCTGGACCGGCTCGGTGGGGTGGAAGTTGGTCTGCTGTTAcatg          >  3:487313/1‑272 (MQ=255)
                                                                                                                                                                                                                                                                            ccTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGTTGAGTATGCTGCGTTCCAGCGACGTGCTGGGGCGCCTCGGGGGGGATGAATTTGGCCTGCGGCTACCAGCCTGTAAt  >  3:847263/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                               
GAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAAT  >  NC_000913/2145042‑2145584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: