Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,739,717 C→T 7.0% L5F (CTT→TTT)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,739,7170CT 7.0% 3092.8 / 53.4 1063L5F (CTT→TTT) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base C (563/416);  new base T (2/79);  total (565/498)
Fisher's exact test for biased strand distribution p-value = 2.24e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1102 total aligned reads displayed.

AACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACT  >  NC_000913/3739569‑3739865
                                                                                                                                                    |                                                                                                                                                    
aaCCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGAGAGATATCCCGCTATGACATTCTccc                                                                                                                                                      <  3:251265/149‑1 (MQ=255)
aaCCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTccc                                                                                                                                                      >  1:1699797/1‑149 (MQ=255)
   cAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCCGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttt                                                                                                                                                   >  1:1975478/1‑149 (MQ=255)
     ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                 <  2:2308463/149‑1 (MQ=255)
     ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                 <  1:121029/149‑1 (MQ=255)
     ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                 <  3:891367/149‑1 (MQ=255)
     ggATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                 <  3:83196/149‑1 (MQ=255)
       aTAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTgg                                                                                                                                               <  4:408257/149‑1 (MQ=255)
        tAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGt                                                                                                                                              >  3:2375381/1‑149 (MQ=255)
          aaaTGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGa                                                                                                                                            >  3:753232/1‑149 (MQ=255)
              gTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACaaa                                                                                                                                        <  4:2033953/149‑1 (MQ=255)
               tCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAAt                                                                                                                                       <  4:26836/149‑1 (MQ=255)
                catcccTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATCTCCCGCTATGACATTCTCCCTTTTTGGTGGCAAAtt                                                                                                                                      >  3:1634731/4‑149 (MQ=255)
                 aGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAAttt                                                                                                                                     >  1:1544923/1‑149 (MQ=255)
                    ccTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAcc                                                                                                                                  >  1:162921/1‑149 (MQ=255)
                    ccTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAcc                                                                                                                                  >  1:766254/1‑149 (MQ=255)
                    ccTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAcc                                                                                                                                  >  2:2470449/1‑149 (MQ=255)
                     cTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAccc                                                                                                                                 <  3:1340639/149‑1 (MQ=255)
                     cTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTAccc                                                                                                                                 <  4:1351147/149‑1 (MQ=255)
                         tCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCa                                                                                                                             >  1:1266814/1‑149 (MQ=255)
                         tCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGAGACAAATTTACCCGCCa                                                                                                                             >  3:2019151/1‑149 (MQ=255)
                                tGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGc                                                                                                                      >  4:558402/1‑149 (MQ=255)
                                     gATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTAc                                                                                                                 >  2:1420295/1‑149 (MQ=255)
                                       tAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCg                                                                                                                        >  4:1013234/1‑140 (MQ=255)
                                       tAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGc                                                                                                               >  2:2234611/1‑149 (MQ=255)
                                            cACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTg                                                                                                          >  1:2400291/1‑149 (MQ=255)
                                             aCCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGa                                                                                                         >  4:1153205/1‑149 (MQ=255)
                                              ccGCTCATTCTCCAACAGACCTATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATACCCCGCTATGACATTCTCCTTTTTTGGTAAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGAt                                                                                                        <  2:1801693/149‑1 (MQ=255)
                                                  tCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGaa                                                                                                    >  1:841812/1‑149 (MQ=255)
                                                  ccACTCTTCAACAGACCGATTTGCCCCCCAGCAAATGTGGCGTTATTGTTACTTTCCTTGCTAAAGAGTTCGACAGATACCCCGCTATGACATTCCCCCTTTTTGGTGAAAAATTTACCCGCCACTCCGTCATTACGCTGTTGATGGaa                                                                                                    <  3:1437965/148‑1 (MQ=255)
                                                   cACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCttttt                                                                                                                                                 >  4:2205458/1‑103 (MQ=255)
                                                    aCTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGA                                                                                                  >  1:2170487‑M1/1‑149 (MQ=255)
                                                      tctTCAACAGACCGATTTGCACCGCAGCAAATGTAGCTTTATTGTTACCTTCCTTGCTACAGAGTTCGAAAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATC                                                                                                <  3:530542‑M1/149‑1 (MQ=255)
                                                      tctTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATC                                                                                                >  2:1532017‑M1/1‑149 (MQ=255)
                                                      tctTAAACAGCCCGATTTGCACCCCGGCAAGTGTAGCGTTATTTTTACCTTCCTTGCTAAAGGGTTCGACAGATATCCCGTTATGACATTCTCCCTTTTGGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATC                                                                                                <  3:493605‑M1/149‑1 (MQ=255)
                                                        ttCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTG                                                                                              >  4:1626813‑M1/1‑149 (MQ=255)
                                                        ttCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTG                                                                                              >  2:1953812‑M1/1‑149 (MQ=255)
                                                         tCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGGTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCTGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGa                                                                                             >  3:1945496/1‑149 (MQ=255)
                                                          cAACAGACCGTTTTGCACCCCAGCAAATGTAGCTTTATTGTTCCCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCCCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGaa                                                                                            <  4:893284/149‑1 (MQ=255)
                                                          cAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGaa                                                                                            <  3:320145/149‑1 (MQ=255)
                                                          cAACAGACCGATTTCCACCCCAGAAATTGTAGCTTTTTTGTTACCTTCCTTCTTACAGAGTTCGACAGATACCCCGTTATGACATTCTCCTTTTTTGGGCACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGaa                                                                                            <  4:2395343/149‑1 (MQ=255)
                                                           aaCAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTCCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAac                                                                                           <  3:2086277/149‑1 (MQ=255)
                                                              aGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAacgac                                                                                        >  1:686122/1‑149 (MQ=255)
                                                                  cGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTGCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGtt                                                                                    <  1:392687/149‑1 (MQ=255)
                                                                    aTTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGCCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTa                                                                                  >  1:536806/1‑149 (MQ=255)
                                                                       tGCCCCCCAGAAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATACCCCGCTATGACTTTCTCCTTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgc                                                                               <  2:1871698/149‑1 (MQ=255)
                                                                        gCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgca                                                                              >  4:1856194/1‑149 (MQ=255)
                                                                          aCCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGTCGGTTTacgcacg                                                                            >  4:1556081/1‑149 (MQ=255)
                                                                          aCCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgcacg                                                                            >  1:2073128/1‑149 (MQ=255)
                                                                           ccccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTacgcacgc                                                                           >  3:669826/1‑149 (MQ=255)
                                                                            cccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGcc                                                                          >  3:2313230/1‑149 (MQ=255)
                                                                            cccAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGACATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGcc                                                                          >  2:959720/1‑149 (MQ=255)
                                                                                gCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGgc                                                                      <  1:1910036/149‑1 (MQ=255)
                                                                                  aaaTGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGAGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGgcgc                                                                    >  4:1956803/1‑149 (MQ=255)
                                                                                     tGTAGCTTTATTGTTACCTTCCTTGCTAAAGAGTTCGACAGATACCCCGCTATGACATTCTCCTTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGAt                                                                 <  4:672041/149‑1 (MQ=255)
                                                                                     tGTAGCGTTATTTTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCTTTTTTGGTGACAAATTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGAt                                                                 <  1:1805009/149‑1 (MQ=255)
                                                                                       tAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGTTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTa                                                               <  2:2400291/149‑1 (MQ=255)
                                                                                       tAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTa                                                               <  2:835239/149‑1 (MQ=255)
                                                                                        ggctttATTGTTCCCTTCCTTGCTACAGAGTTCGCAAGATATCCCGCTATGACATTCTCCCTTTTTGGTAAAAAATTTACCCGCCACACCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTAt                                                              <  2:1666474/145‑1 (MQ=255)
                                                                                        aGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATACCCCGCTATGACATTCCCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTAt                                                              <  3:1276926/149‑1 (MQ=255)
                                                                                                  ttACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcggcggc                                                    >  1:2278005/1‑149 (MQ=255)
                                                                                                  ttACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcggcggc                                                    >  2:130512/1‑149 (MQ=255)
                                                                                                   tcccttccttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcggcggcg                                                   <  1:1127478/147‑1 (MQ=255)
                                                                                                     ccttccttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGt                                                 >  2:2034918/1‑149 (MQ=255)
                                                                                                      cttccttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTa                                                >  4:1233771/1‑149 (MQ=255)
                                                                                                          cttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTcggcggcggagatcg                                            >  3:1765617/1‑143 (MQ=255)
                                                                                                          cttGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATcc                                            >  2:1171390/1‑149 (MQ=255)
                                                                                                               tACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGc                                       <  4:355967‑M2/149‑2 (MQ=255)
                                                                                                               tACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGca                                      <  3:2228095‑M2/149‑3 (MQ=255)
                                                                                                                aCAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGca                                      <  2:420924‑M2/149‑3 (MQ=255)
                                                                                                                   gagTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGcagat                                   <  3:1043409‑M2/149‑6 (MQ=255)
                                                                                                                      ttCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATccc                                >  3:2151532/1‑149 (MQ=255)
                                                                                                                       tCGACAATTACCCCGTTATGACTTTCCCCTTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCg                               <  2:635352/149‑1 (MQ=255)
                                                                                                                       tCGAAAGATACCCCGCTATGACTTCCTCCCTTTTTGGTGACAAATTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCg                               <  4:1713266/149‑1 (MQ=255)
                                                                                                                        cGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCgg                              >  1:61242/1‑149 (MQ=255)
                                                                                                                        cGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCgg                              >  1:1033450/1‑149 (MQ=255)
                                                                                                                         gACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGa                             >  3:1861098/1‑149 (MQ=255)
                                                                                                                         gACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGa                             >  4:1487651/1‑149 (MQ=255)
                                                                                                                           cAGATATCCCGCTATGACATTCTCCCTTTTTGGTAACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGaaa                           <  2:762677/149‑1 (MQ=255)
                                                                                                                             gATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATg                         <  1:465742/149‑1 (MQ=255)
                                                                                                                              atatCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGc                        <  2:1135261/149‑1 (MQ=255)
                                                                                                                                atCCCGCTATGACTTTCTCCCTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAg                      <  2:2179735/149‑1 (MQ=255)
                                                                                                                                atCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAg                      <  3:392628/149‑1 (MQ=255)
                                                                                                                                atCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAg                      >  2:1633356/1‑149 (MQ=255)
                                                                                                                                atCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCT                                                                                               >  2:501659‑M1/1‑76 (MQ=255)
                                                                                                                                  cccGCTATGACATTCTCCTTTTTTGGTGAAAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGa                    <  2:313922/149‑1 (MQ=255)
                                                                                                                                  cccGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGa                    <  4:2129714/149‑1 (MQ=255)
                                                                                                                                  cccGCTATGACATCCCCCTTTTTTGGTGACAACTTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGTAAATGCAGGa                    <  2:2126959/149‑1 (MQ=255)
                                                                                                                                   ccGTTATGACTTTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGac                   <  3:1541677/149‑1 (MQ=255)
                                                                                                                                   ccGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGac                   >  2:1056812/1‑149 (MQ=255)
                                                                                                                                      cTATGACATTCTCCCTTTTGGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGactac                <  4:2289690/149‑1 (MQ=255)
                                                                                                                                       tATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGactact               >  3:944667/1‑149 (MQ=255)
                                                                                                                                         tGACATCCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTc             <  1:955823/149‑1 (MQ=255)
                                                                                                                                           cctttttcCTTTTTTGGTAACAATTTTAGCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCa           <  1:2479689/143‑1 (MQ=255)
                                                                                                                                             aTTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCa           >  4:809634/1‑147 (MQ=255)
                                                                                                                                             aTTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCAGAACGACGGTTTACGCACGCCTGGTGCGATTATGCTCGGCGGCGGTAATCCGGCACAGATACCGGACATGCAGGACTACTTGCAGa         >  4:1306836/1‑149 (MQ=255)
                                                                                                                                                ctcCCTTTTTGGTGAAAATTTTCCCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGc      <  2:897345/149‑1 (MQ=255)
                                                                                                                                                 tcCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCt     >  1:299566/1‑149 (MQ=255)
                                                                                                                                                 tcCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCt     >  1:1439459/1‑149 (MQ=255)
                                                                                                                                                   ccTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTAc   >  3:1102821/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACCAGGATAAAATGTCAGCCCTAATCAGCGTTGCAGGATAAAGCACCGCTCACTCTTCAACAGACCGATTTGCACCCCAGCAAATGTAGCGTTATTGTTACCTTCCTTGCTACAGAGTTCGACAGATATCCCGCTATGACATTCTCCCTTTTTGGTGACAAATTTACCCGCCACTCCGGCATTACGCTGTTGATGGAAGATCTGAACGACGGTTTACGCACGCCTGGCGCGATTATGCTCGGCGGCGGTAATCCGGCGCAGATCCCGGAAATGCAGGACTACTTCCAGACGCTACT  >  NC_000913/3739569‑3739865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: