Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,115,901 G→A 100% A441V (GCA→GTA)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,115,9010GA100.0% 1041.9 / ‑6.7 290A441V (GCA→GTA) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (144/145);  minor base C (0/1);  total (144/146)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 290 total aligned reads displayed.

CTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCC  >  NC_000913/4115755‑4116044
                                                                                                                                                  |                                                                                                                                               
 ttttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACgg                                                                                                                                              >  4:2067525/1‑149 (MQ=255)
  tttttGCAGCCTACGTAGCGGTAATTACGATCAGTGGTTTCGATGCCTGGAAGGATCTCGCGCTCGATCATCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCAAAGCGGGAACCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  4:2263175/149‑1 (MQ=255)
  tttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  1:489964/149‑1 (MQ=255)
  tttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  3:1127235/149‑1 (MQ=255)
  tttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  3:2260516/149‑1 (MQ=255)
  tttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  1:1346914/149‑1 (MQ=255)
  tttttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGt                                                                                                                                             <  3:1450717/149‑1 (MQ=255)
     ttCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGAc                                                                                                                                          <  3:2069824/149‑1 (MQ=255)
      tCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACt                                                                                                                                         >  2:1997146/1‑149 (MQ=255)
       ccAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACtt                                                                                                                                        <  4:1316966/149‑1 (MQ=255)
          gCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTcgc                                                                                                                                     <  3:1151321/149‑1 (MQ=255)
                  aaCGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTcc                                                                                                                             <  4:490850/149‑1 (MQ=255)
                     ggTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCggg                                                                                                                          >  1:2032618/1‑149 (MQ=255)
                     ggTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCggg                                                                                                                          >  3:642506/1‑149 (MQ=255)
                      gTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGgc                                                                                                                         <  1:1290908/149‑1 (MQ=255)
                      gTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGgc                                                                                                                         >  3:593264/1‑149 (MQ=255)
                      gTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCCCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGgc                                                                                                                         >  4:1911122/1‑149 (MQ=255)
                              gCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAAcg                                                                                                                 >  1:1015556/1‑149 (MQ=255)
                              gCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAAcg                                                                                                                 >  2:722809/1‑149 (MQ=255)
                               cTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTACTCTGGCAGCTCGTCGAGGTTCTCCCAGAAGCCAACCGCCAGGCCTGCTAGATAGGCCGCACCCAATACGGTGACTTCGCTCACTTCCGGGCGCTCAAcgc                                                                                                                <  4:121144/149‑1 (MQ=255)
                                  aGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCggg                                                                                                             >  3:2326316/1‑149 (MQ=255)
                                  aGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCggg                                                                                                             >  1:1176684/1‑149 (MQ=255)
                                     ggTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGc                                                                                                          <  2:2239321/149‑1 (MQ=255)
                                       tttCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCg                                                                                                        >  4:1871578/1‑149 (MQ=255)
                                        ttCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCga                                                                                                       <  4:329301/149‑1 (MQ=255)
                                        ttCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCga                                                                                                       <  2:480217/149‑1 (MQ=255)
                                        ttCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCga                                                                                                       <  2:615809/149‑1 (MQ=255)
                                           gATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGaa                                                                                                    >  2:336855/1‑149 (MQ=255)
                                            aTGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACCCGGGTGCCGAGAat                                                                                                   <  3:177635/149‑1 (MQ=255)
                                             tGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAata                                                                                                  >  1:2293751/1‑149 (MQ=255)
                                              gCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAatat                                                                                                 <  1:762787/149‑1 (MQ=255)
                                              gCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAatat                                                                                                 <  1:595629/149‑1 (MQ=255)
                                               ccTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATc                                                                                                <  3:76872/149‑1 (MQ=255)
                                               ccTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATc                                                                                                <  3:184370/149‑1 (MQ=255)
                                                cTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCg                                                                                               >  1:853058/1‑149 (MQ=255)
                                                   gACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGAc                                                                                            >  2:1211276/1‑149 (MQ=255)
                                                   gACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGAc                                                                                            <  1:159795/149‑1 (MQ=255)
                                                    aCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACt                                                                                           <  1:705462/149‑1 (MQ=255)
                                                    aCGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACt                                                                                           <  1:387726/149‑1 (MQ=255)
                                                         aCTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAAc                                                                                      <  2:1493312/149‑1 (MQ=255)
                                                           tCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTg                                                                                    >  4:589232/1‑149 (MQ=255)
                                                           tCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTg                                                                                    >  2:1446121/1‑149 (MQ=255)
                                                               gcTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATc                                                                                >  3:1898409/1‑149 (MQ=255)
                                                               gcTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATc                                                                                >  3:879026/1‑149 (MQ=255)
                                                                 tCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGCACTGGAACTGCATCAg                                                                              >  3:2090725/1‑149 (MQ=255)
                                                                   aaTCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGa                                                                            >  3:1377401/1‑149 (MQ=255)
                                                                    aTCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaa                                                                           >  3:907364/1‑149 (MQ=255)
                                                                    aTCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaa                                                                           >  3:1004098/1‑149 (MQ=255)
                                                                     tCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaaa                                                                          <  4:712628/149‑1 (MQ=255)
                                                                       aCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAAtt                                                                        >  1:1912777/1‑149 (MQ=255)
                                                                       aCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAAtt                                                                        >  1:2464421/1‑149 (MQ=255)
                                                                       aCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAAtt                                                                        >  1:1477218/1‑149 (MQ=255)
                                                                        ccGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCg                                                                                               >  2:814105/1‑125 (MQ=255)
                                                                          gCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGtt                                                                     <  2:2280876/149‑1 (MQ=255)
                                                                           cTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGttt                                                                    <  2:1632741/149‑1 (MQ=255)
                                                                            tttCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTg                                                                   <  4:779593/149‑1 (MQ=255)
                                                                            tttCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTg                                                                   <  1:1251193/149‑1 (MQ=255)
                                                                               ctctTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTa                                                                <  3:2012002/149‑1 (MQ=255)
                                                                                     cAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTgcgc                                                          <  3:2049961/149‑1 (MQ=255)
                                                                                      aGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGcc                                                         <  1:1793054/149‑1 (MQ=255)
                                                                                         tcgtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGccacc                                                      >  3:281579/1‑149 (MQ=255)
                                                                                           gtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCAt                                                    >  4:1798896/1‑149 (MQ=255)
                                                                                           gtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCAt                                                    >  1:2197264/1‑149 (MQ=255)
                                                                                           gtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCAt                                                    >  4:1490692/1‑149 (MQ=255)
                                                                                             cgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATcc                                                  <  3:476864/149‑1 (MQ=255)
                                                                                             cgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATACCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATcc                                                  <  2:1255083/149‑1 (MQ=255)
                                                                                             cgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACATCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATcc                                                  <  3:315017/149‑1 (MQ=255)
                                                                                              gAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCa                                                 >  1:1679860/1‑149 (MQ=255)
                                                                                               aGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAc                                                >  4:1061795/1‑149 (MQ=255)
                                                                                                ggTTCTGGCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAcg                                               >  2:1350156/1‑149 (MQ=255)
                                                                                                     tGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAg                                          <  1:1442593/149‑1 (MQ=255)
                                                                                                      gCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAgg                                         <  1:2098406/149‑1 (MQ=255)
                                                                                                        cAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGc                                       <  4:1973556/149‑1 (MQ=255)
                                                                                                        cAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGc                                       >  1:1004452/1‑149 (MQ=255)
                                                                                                           aaGCTAACCGCCAGGCCTGCGAGATAGGACGGACCCAATACGGTGACTTCCCGCACTTGCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTg                                    <  4:1583808/149‑1 (MQ=255)
                                                                                                               cAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGa                                >  3:1822514/1‑149 (MQ=255)
                                                                                                                 aCCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCGCCATCCACGCGCAGGGCGTGCAGACg                              >  1:1712009/1‑149 (MQ=255)
                                                                                                                  ccGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTg                                                                   >  1:1561385/1‑111 (MQ=255)
                                                                                                                     ccAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATa                          <  4:879026/149‑1 (MQ=255)
                                                                                                                        ggCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCa                       >  2:1750964/1‑149 (MQ=255)
                                                                                                                        ggCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCa                       <  3:1700070/149‑1 (MQ=255)
                                                                                                                          ccTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTTCTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCaga                     <  2:562831/149‑1 (MQ=255)
                                                                                                                          ccTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCaga                     <  4:632512/149‑1 (MQ=255)
                                                                                                                            tGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGt                   >  4:1084772/1‑149 (MQ=255)
                                                                                                                            tGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGt                   >  1:2354604/1‑149 (MQ=255)
                                                                                                                               gagaTAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCgg                <  2:1524634/149‑1 (MQ=255)
                                                                                                                                 gaTAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGcc              <  2:873406/149‑1 (MQ=255)
                                                                                                                                  aTAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCt             <  4:1700985/149‑1 (MQ=255)
                                                                                                                                       ccGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCagag                    <  1:991104/137‑1 (MQ=255)
                                                                                                                                       ccGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATc        >  2:2456717/1‑149 (MQ=255)
                                                                                                                                         gCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACg                              <  3:1064782/125‑1 (MQ=255)
                                                                                                                                            cccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTc   <  3:2220091/149‑1 (MQ=255)
                                                                                                                                            cccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTc   <  2:1368180/149‑1 (MQ=255)
                                                                                                                                            cccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTc   <  3:1540264/149‑1 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGGCGGATACCAGAGTCGGCCTGCATCGCTTcc  <  2:1858974/149‑1 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTcc  <  2:1015556/149‑1 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTcc  >  4:40867/1‑149 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTcc  >  2:2203715/1‑149 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTGcc  >  3:1629425/1‑149 (MQ=255)
                                                                                                                                             ccAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCGGCATCGCTTcc  <  4:230979/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCC  >  NC_000913/4115755‑4116044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑