Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,463,481 | Δ1 bp | 6.7% | intergenic (+43/‑58) | paaK → / → paaX | phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,463,481 | 0 | G | . | 6.7% | 452.8 / 27.6 | 134 | intergenic (+43/‑58) | paaK/paaX | phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer |
Reads supporting (aligned to +/- strand): ref base G (58/66); new base . (4/5); total (62/72) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-02 |
Only 100 of 138 total aligned reads displayed.
GGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGTCTTAATAAAGAAG > NC_000913/1463218‑1463740 | gggggCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGAc < 4:376378/274‑1 (MQ=255) ttCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGAGGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATCTTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGGAAAGGGCCAGGGCCAGAAGTCGATACGAACTGGTCTATGATTCAGaaac > 4:301064/1‑274 (MQ=255) gtgAAAGTGGAGTGGAAAGAAAGTAGCCGGAAATTGCGGCATGGGGAGCGTTGCCAGGTATGCCAACAGCTGCTCCATCGGATTAAGTCGATGGTAGGGATCTCTACCG‑ATGTGATGATCGTTAACGTTGGCAGTCTCCCGCGTTCAGAAGGCAAGGGGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATcaca < 4:535740/275‑1 (MQ=255) aCATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGt < 2:445280/274‑1 (MQ=255) agcagcGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGAACTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGTCCTGAAGTCGATACGACCTGTCCTATGAGTCATACACAACAACAATAACAACAGAACGATGCGAAGGAGTAAACttaaaccctt > 4:451895/1‑267 (MQ=255) aagagacagaaccaGCTGGGGCAGCGGATTAAGGTGAGTGTGGGGATTCCTACCG‑ATGTGATGATCGTTGACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGAGTTTCGGGTGTTTGATCTGCGAAATATTGTTGGGGACTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGAACTGTGCTATGAGTCATAAATCAGAACAAGAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACAt < 4:359168/264‑1 (MQ=255) cgcCATCGGTCTAAGTCGATGGTGGGTCGCTCTACCG‑ATGGGACGCTCGTTAACTGTGTCAGTATACCACGGTCAGAAGGTAAGGCGTGTCGGGTGTTTGATCAGCGCAATATTGTTGGTGCCGCACGTTTCGTCTGGCCCTGGTGGGGTAAAGCGCCAGCGCCAGAAGTCGATACGACCTGTGCGATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAAGGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCg < 4:260985/274‑1 (MQ=255) cgatggatttgaGTCGGTGGTGGGGCATTCTACCGAAGGTGA‑GAGCGTGAGCGGTGGCAGTAACCCGCGTTCGGAAGGAAAGGCGTGTCGGGGATTTGATCTGGGAAATATTGGTGGTGCCTGACTGATCGGCTGGCCCTGGTGGGTTAAACCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGt < 4:512521/263‑1 (MQ=255) ccATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGCCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTTCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCTGGGCCAGAAGTCGATACGACCTTCGCTATCATTCATACATCACAACAATAACAACCGACTGAAGCGAATGAGTCAACTTGCTACTTTTATCCAACATGCTGTAAACGcctgtctctt > 2:359169/1‑265 (MQ=255) ggATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTTCGGTCAGTg > 2:269133/1‑274 (MQ=255) gATTAAGTTGATGGTGGGGATCTCTACCG‑ATGGGATGTTCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGGGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGc < 4:232675/275‑1 (MQ=255) gtaggtggttgttggGGTTTTCTACGG‑ANNNGATGTTGGTGACCTTTGGCAGTATCCCGCGTTCAGAAGGAAAGGCGTGTCGGGTGTTTGATTTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGca < 3:157125/262‑1 (MQ=255) aaGTCGATGGTGGGGATCTCTACCG‑ATGTGTTGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGca < 2:433895/272‑1 (MQ=255) tggtggGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGAATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAt < 2:506797/275‑1 (MQ=255) ggggATCTTTACCG‑ATGTGAGGTTCGTTAACTGTGGCAGTATCCCGCTTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATctcc < 4:476996/275‑1 (MQ=255) gatgtcaacgtgtgtgAGGAGCGTTAACGGTGGCAGTAGCCTGCGTTCAGAAGGAAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGGGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCctc‑t < 1:154008/263‑1 (MQ=255) tctcTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatac < 6:973960‑M2/148‑7 (MQ=255) tctACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGa < 5:1008694/148‑1 (MQ=255) gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTg > 4:40740/1‑221 (MQ=255) gtgATGATCGTTAACTGTGGCAGTATCACGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTa > 4:458063/1‑223 (MQ=255) tgatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa < 2:40741/221‑1 (MQ=255) gatCGTTAACTGTGGAAGTATCCCTCGTTCAGAAGGAAAGGCGTGTCGGGTGTTTGAGCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCtt < 2:235219/275‑1 (MQ=255) aCTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTAGTGCCTGACGTATCGTCTGGCTCTGGTGGTGTATAGCGCCAGGGGCAGAAGCCGATTCGACTTTTACTATGATTGTTAAATCACAACAAAAACAACAGACTAAAGCGAATGAGTAAAATTAATACTTATATCCAACATGCTGTCAACGCAGTTCCGGTCAGTGGCCCATCTTTTATCTCCTA‑CATGTATGTCGATTcctttgctaa > 4:234484/1‑263 (MQ=255) ggCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACTTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGaa > 4:456619/1‑165 (MQ=255) ggCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg < 8:496530/132‑1 (MQ=255) cAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCAcaacaa > 5:409625/1‑148 (MQ=255) aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAAt > 5:1043294/1‑148 (MQ=255) gTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa < 2:456620/165‑1 (MQ=255) tCCCGCGTCCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCTCTGGTGGGGTAACGCGCCAGGGCCAGCAGTCGATACGACCTGTGCTATGATTCATGAATCACAACAATAACAACAGACTGAAGGGAATGAGTAAACTTGATACGTTTACACAACCATCTGTAAACGCTGTTCCGGTCTGTTGCTCATCTTTGATCTCCTC‑CCTGTATTGTGATTCGTTTTCCCAttgggtgtgtgaa > 4:147888/1‑261 (MQ=255) cccGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATc < 2:451896/275‑1 (MQ=255) cgTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGTCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAATTGATACTTTTATCCAACATGCTGTAAACGCTGTTCAGGTCAGTGGCACATATTTGATCACATC‑TCTGTAAGGTAATTCGCTTTCTCATCGTGGTGGTGAAATCTGGt > 4:353122/1‑275 (MQ=255) tCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTgg > 2:72073/1‑275 (MQ=255) ggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAAAGAGAAAATTTGATACTTTTATCCAACATACTGTAAACGCTGTTCCGGTATGTGGAACATCTTTGAT‑TCATCATCTGTATGGTTTTTTGCTCTATCATANNNGTGGTGAAACTTGGCTggttggtacggcg > 3:147022/1‑264 (MQ=255) tgtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCtt > 2:7161/1‑275 (MQ=255) tCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGCCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGAtt > 2:47293/1‑225 (MQ=255) cGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAg < 5:171501/79‑1 (MQ=255) gtgtTTGAGCTGCGTAAGATTTGTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGCCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCt < 4:47292/225‑1 (MQ=255) gATCTTCTCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCGGGCTGCTTTGCTGGAAggg < 2:234485/274‑1 (MQ=255) gcgcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgata < 4:29631‑M2/71‑6 (MQ=255) cgcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCAGAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATcc > 2:547721/1‑146 (MQ=255) gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAg < 5:447631/110‑1 (MQ=255) gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAg > 6:447631/1‑110 (MQ=255) gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGAt < 2:150461/274‑1 (MQ=255) aaTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGAc < 2:29632/71‑1 (MQ=255) atatTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCAGAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa < 4:547720/146‑1 (MQ=255) gTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCAAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCTCTTCTTTGATCACCTC‑TCTGTATGGTAATTCGCTTTCCCATCGTGGTGGTTAACGCTGTTTGGGTGTGCTGCGTTGTTTGCTGGCAGGGCGGGGTTgtggtggacggtc > 4:407249/1‑268 (MQ=255) gCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAAAAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACGTGCTGTAAACGCTGTTCCGGTCAGTTGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAATTCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTGGGTGAGCGTTTCg > 2:32843/1‑275 (MQ=255) cTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTg < 1:147022/275‑1 (MQ=255) tGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggta < 6:396354‑M2/148‑102 (MQ=255) gACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggata > 7:343372‑M2/1‑46 (MQ=255) gACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggata < 8:343372‑M2/117‑72 (MQ=255) aCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa < 5:1061698/101‑1 (MQ=255) aCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa > 6:1061698/1‑101 (MQ=255) cGTATCGTCTGGTCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCa < 2:353123/275‑1 (MQ=255) aTCGTCTGGCCCCGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGATGGGCTGGGATTTGGTGAGCGTTTccttccccccg > 2:457043/1‑265 (MQ=255) tCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGCGATTCGCTTTCCCCTCGTGGTGGTGAAATATGGTTGGGTAGTCTGGCCGCTTTGCTGGCAGGGCGGGGGTTTGGGGAGGTTTTCGTGCGCACCGc > 4:393446/1‑275 (MQ=255) gtatGGCCCTGGTGGGGTAAAGCGGCAGGGCCAGAAGTGGATACGAACTGTGCTATGATTCATAAAGCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCt < 1:145765/271‑1 (MQ=255) gTCTGGCCCTGGGGGGGTAAAACGCCAGGGCCAGAAGGCGATACGACCTTTGCTATGATTCATAAATCACAACAATAACAACAGACTGAATCGAATGAGTAAACTTGATACTTTTAGCCAACGTGCTGTAAACGGTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGGTTCGCTTTCCCATCGTGGTGGGGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCt < 4:32842/274‑1 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa < 7:1056002/77‑1 (MQ=255) tCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa > 8:1130786/1‑77 (MQ=255) cTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 5:159422/110‑1 (MQ=255) cTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 6:159422/1‑110 (MQ=255) tGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcgcatcggtagagatccccaccatc < 6:1002090‑M2/148‑116 (MQ=255) ggCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaa < 7:532854‑M2/115‑84 (MQ=255) ggCCCTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaa > 8:532854‑M2/1‑32 (MQ=255) gCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc > 7:523393/1‑106 (MQ=255) gCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc < 8:523393/106‑1 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTcgatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatccccaccatcga > 5:396354‑M2/1‑30 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 5:974691/104‑1 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatcc > 5:962068‑M2/1‑28 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatcc < 6:962068‑M2/135‑108 (MQ=255) cTGGTGGGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaa > 5:468484‑M2/1‑28 (MQ=255) cTGGTGGGGTAAAACGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGt < 4:72072/275‑1 (MQ=255) tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa < 6:690522/123‑1 (MQ=255) tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa > 5:690522/1‑123 (MQ=255) gtggGGTAAAGCGCCAGGGCCAGA‑‑gatacgtcaggcaccaacaatattgcgcagatcaaacacccgacacgccttgccttctgaacgcgggatactgccacagttaacgatcatcacatcggtagagatccccaccatcgacttaat > 7:187228‑M2/1‑25 (MQ=255) gggggTAAAGGGCCAGGGCCAGAATTCGATACGACCTGTGCTATTATTCAAAAATAAAAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGTCTta < 4:7161/274‑1 (MQ=255) ggggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGa < 6:657789/148‑1 (MQ=255) ggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGTCTtaata > 2:303786/1‑275 (MQ=255) gTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTcc < 5:949017/125‑1 (MQ=255) gTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGGGAGCGTTTCGTGCGCa > 4:353079/1‑253 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt < 6:474432/88‑1 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt > 5:474432/1‑88 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg > 7:961670/1‑130 (MQ=255) tAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtctc > 5:291769/1‑148 (MQ=255) aaaGCGCCAGGGCTAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa > 7:77455/1‑114 (MQ=255) aaaGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa < 8:77455/114‑1 (MQ=255) aGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGa > 7:112128/1‑69 (MQ=255) aGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGa < 8:112128/69‑1 (MQ=255) gcgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACCGACTGAAGCGAATGAGTAAACTTg < 6:417160/86‑1 (MQ=255) gcgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGTCTTAATAaagaag > 2:323775/1‑275 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 7:355096/1‑90 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 6:302089/90‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 8:355096/90‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTa > 8:788131/1‑94 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTa < 7:788131/94‑1 (MQ=255) cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaa > 6:108851/1‑109 (MQ=255) gAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt > 8:1118803/1‑79 (MQ=255) gAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt < 7:1118803/79‑1 (MQ=255) gTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCg < 6:291769/148‑1 (MQ=255) | GGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCG‑ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTC‑TCTGTATGGTGATTCGCTTTCCCATCGTGGTGGTGAAATCTGGTTGGGTAGTCTGGCTGCTTTGCTGGAAGGGCTGGGATTTGGTGAGCGTTTCGTGCGCACCGCTTTGTTTCGTCTTAATAAAGAAG > NC_000913/1463218‑1463740 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |