Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,868,865:1 | +G | 5.7% | intergenic (+23/‑90) | yeaG → / → yeaH | protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,868,865 | 1 | . | G | 5.7% | 454.1 / 22.3 | 105 | intergenic (+23/‑90) | yeaG/yeaH | protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein |
Reads supporting (aligned to +/- strand): ref base . (45/54); new base G (3/3); total (48/57) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01 |
Only 100 of 107 total aligned reads displayed.
CGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAC > NC_000913/1868593‑1869138 | ccAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TAc < 4:212814/274‑1 (MQ=255) gACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTCCGTTTACTGTTCGAATGGTATTTGCGCGTACGTAAATCGTCTTCACAACCATGGTCCGGTACCCATGT‑TACCGGGCCTACAACgtccgc > 2:16309/1‑269 (MQ=255) aCGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCg < 2:378265/274‑1 (MQ=255) gCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGaa < 2:229106/274‑1 (MQ=255) gCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg > 2:104162/1‑274 (MQ=255) aTGAAAAACTGGGCACTGTAATCGGGAAGAAAAGGTTCTCAAGTACCGGGGGGCTTTTGCCGGTTATCTCGTTGAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGGATTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGGGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCaa < 4:16309/275‑1 (MQ=255) ggaaaaCTGGTAACGTTCATCGAGAAGAAAATGTTGTCCATGACCGAGAAGCGGTTGCCGGTTATCTCGGTTGACGCCGAAACGTCAACCGACGAGCAGAAGAAACCCGACGACTTGGTCGACCGTATGATGGAAAAAAGCTACACCCGTAAGCAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACAGGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAaca < 4:410013/273‑1 (MQ=255) aCTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTTCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACACGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGACAAGCGGCaaacccagc > 4:439819/1‑268 (MQ=255) cTGCGGACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCAAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGc < 2:3850/274‑1 (MQ=255) tCATCGGAAAGAAAATATTCTCCAATACCGAGGAGCTGTTGCCGGTTCTCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACCCGACGACTTTGTCGTCCTTATGATGGAAAAAAGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAg < 1:2480/275‑1 (MQ=255) gagaAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc < 4:104161/275‑1 (MQ=255) gagaAGAAAATGTTCTCCAAGACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACGTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTAGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc < 2:303262/275‑1 (MQ=255) gaaaagacAATGTGATCCAATCCCGAGTATCTGTTTCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACTACTTTGTCGACCGTATGATGGAAAAAGGCGACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc < 1:47162/272‑1 (MQ=255) agaagaAAAAGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa < 2:525700/275‑1 (MQ=255) aagaaAATGTTCTACAATACCGAGGAGCTGTTGACGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACGGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaa < 2:264293/274‑1 (MQ=255) ctcccatgcccgggagCTTTTGCTGGTTATCTCGTTTAACGCAAAAACGTCCCCCGACGAGCAGTAGAATCACGACGACTTTTTCACCCGTATGATGGAAAAGGGCTACACCCGTAAAAAGATGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg < 4:380029/261‑1 (MQ=255) tcCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACgggg < 4:199557/275‑1 (MQ=255) ggggcggtggCCGGTAATATCGTTTAACGCCAAAACGTCAACGGACGAGCAGAAGAAAAACGACGACTTTGTCGAACGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc < 4:84543/265‑1 (MQ=255) cTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGCGGCCCTGAGAAGCGGCAACCGCGGCGTAGCCTACAGTTGGCAAATGTCCTACGGGCGGACTCTGACCTgg > 4:261404/1‑274 (MQ=255) cGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACGGCGAACCGTGGGCCTGAGAAGCg > 2:562426/1‑195 (MQ=255) aaanNNTCAACAGACGGGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACGGCGAACCGTGGGCCTGAGAAGCGGCaa < 4:562425/195‑1 (MQ=255) aCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACa < 2:439820/275‑1 (MQ=255) aCGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACa < 2:68845/275‑1 (MQ=255) cggcAACCGACGAGAGAAAAAAAGCCTACGACTTTTTATACCGGATTATGGAAAAAGGCTCTCCCCGTAAAGAGGTTCGTTTAGTGTGCTAATGGTATGTGGNCGGACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt < 3:112307/238‑1 (MQ=255) gacTTTGTCGACCGTATGATGGAAAAAGGCTACGCACGTAAACAGGTGCGTGTACTGTGCAAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAAACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa < 2:508266/274‑1 (MQ=255) acTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCg > 6:672405/1‑120 (MQ=255) acTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCg < 5:672405/120‑1 (MQ=255) cgttGTCGAGAGTAGGATGGAAAAAGGCAACACCGTTAACAAGGTGAGTTTACTGTTCGAATGTTATGTGGACGTACGTAAATAGTCGTAAAAACCCGGGCCCGGTACACAGGT‑TACCGGTCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGGTGGCAAATGTAGTACGGGGGGCATATGAACTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACg < 4:258474/272‑1 (MQ=255) tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAGACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCg > 7:787217/1‑116 (MQ=255) tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAGACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCg < 8:787217/116‑1 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCgg > 7:684372/1‑105 (MQ=255) gatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGTGTACCgg < 8:684372/105‑1 (MQ=255) aaaaaGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACCCAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTTACCGGCCTCTGAACGGCAAAAACAAACGCATGGTGAATCGCCAGCGCTTTTTTCGCCGTTATAAAGGCCAAATTa > 4:67707/1‑274 (MQ=255) aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACa < 2:67708/274‑1 (MQ=255) cTACACCCGTAAACAGGTGCTGTTACTGTGCGAATGGTATCTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTc < 2:253195/274‑1 (MQ=255) cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCACGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGNCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGACAGGCAAAAAAAAAAGAATGGTGAATCGCCAGCGgtttagangnn > 3:108510/1‑242 (MQ=255) acacCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttt > 2:492005/1‑241 (MQ=255) ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACg < 4:492004/241‑1 (MQ=255) cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg < 5:719135/120‑1 (MQ=255) cGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg > 6:719135/1‑120 (MQ=255) gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCg > 6:328121/1‑84 (MQ=255) gTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCg < 5:328121/84‑1 (MQ=255) tAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATTGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCCACACCGGCGTAGCATACAGTTGGCAAATTGAGTATGGGGGGCATATGCCCTGGGTTATTGACCGGCGTCTGACCGGCAAAAACAAAAGACTGGTGAATCCCCAACGTTTTTTACGCAGTTATGAAGCACAAATCAAACAGGCGATCCCCGAg > 4:301257/1‑275 (MQ=255) tAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCa > 5:557120/1‑148 (MQ=255) ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGtgta < 5:517956/74‑4 (MQ=255) ggTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTA‑ACATGtgta > 6:517956/1‑71 (MQ=255) gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGt > 2:257892/1‑136 (MQ=255) gTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAACAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta > 2:447990/1‑275 (MQ=255) tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGt < 5:243114/125‑1 (MQ=255) tGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGt > 6:243114/1‑125 (MQ=255) gTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc < 4:257891/136‑1 (MQ=255) gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGTGGGGCATATGACCTGGTTTATTGACCCGCGTCTCAACGGCAAAAACAAAACCCTGGTGAACCGCCAGAGTTTTTTACGCCGTtata > 2:512550/1‑228 (MQ=255) gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCCCCGAGGCCATTAATCAGCGTTc > 2:193848/1‑274 (MQ=255) gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa > 5:955141/1‑103 (MQ=255) gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa < 6:955141/103‑1 (MQ=255) ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac > 7:51799/1‑101 (MQ=255) ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAacac < 8:51799/101‑1 (MQ=255) ggTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt > 8:422050/1‑148 (MQ=255) ttGCGCGTACGTAAATCGTCTTAACAAGCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt > 8:700153/1‑148 (MQ=255) ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg < 6:530979/98‑1 (MQ=255) ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAg > 5:530979/1‑98 (MQ=255) gcgcGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt < 7:422050/148‑1 (MQ=255) cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGa < 6:557120/148‑1 (MQ=255) cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGACTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAAGGGGGGCATATGACCTGGTTGATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGACAGCGTTTTTTACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg > 2:371115/1‑274 (MQ=255) tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAcc < 7:700153/148‑1 (MQ=255) taAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt < 7:966734/96‑1 (MQ=255) taAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt > 8:966734/1‑96 (MQ=255) taAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg < 7:180141/128‑1 (MQ=255) taAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg < 5:1028447/128‑1 (MQ=255) tCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCCAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCATTCGGTGACTGACGTCGACAGCGGCGAATCCGTAt > 2:56959/1‑275 (MQ=255) caacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACCGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt < 7:294393/119‑1 (MQ=255) caacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg > 8:67546/1‑66 (MQ=255) caacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCgg < 7:67546/66‑1 (MQ=255) caacCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAACATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt > 8:294393/1‑119 (MQ=255) cgggCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg < 2:135926/273‑1 (MQ=255) cTGGCCCGGTACAAATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg < 2:384376/275‑1 (MQ=255) ggCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg > 8:478134/1‑58 (MQ=255) ggCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg < 7:478134/58‑1 (MQ=255) ggTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt < 8:44663/112‑1 (MQ=255) ggTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGttt > 7:44663/1‑112 (MQ=255) ggTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGt > 5:1034676/1‑110 (MQ=255) ggTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGt < 6:1034676/110‑1 (MQ=255) cacaTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata > 8:383360/1‑97 (MQ=255) cacaTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata < 7:383360/97‑1 (MQ=255) caactGT‑TACCGGGCCTACAACGCAAGCGGACCGTGGGCCTGGGAAGCGGAAACACAGGGGTAGCATACAGGTGGCAAATGTAGTACGGGTGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGAAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAg < 4:457929/271‑1 (MQ=255) caTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa > 7:711173/1‑136 (MQ=255) caTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa < 8:711173/136‑1 (MQ=255) aTGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGa < 7:987767/97‑1 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc > 7:849024/1‑44 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGc < 8:849024/44‑1 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaa > 5:135788/1‑129 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaa < 6:135788/129‑1 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt < 6:867741/139‑1 (MQ=255) tGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCAt > 5:867741/1‑139 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa < 6:208579/74‑1 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCaaa > 5:208579/1‑74 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg > 6:704857/1‑83 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACg < 5:704857/83‑1 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGc < 8:214512/148‑1 (MQ=255) gT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGACAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc > 2:93173/1‑275 (MQ=255) | CGCCAGAGCGAATAACAGCGGACGCAATCCGAACTGGACCAGCTATGAAAAACTGCGCACGGTCATCGAGAAGAAAATGTTCTCCAATACCGAGGAGCTGTTGCCGGTTATCTCGTTTAACGCCAAAACGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGT‑TACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAC > NC_000913/1868593‑1869138 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |