Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,330,419:1 +T 14.3% intergenic (+23/+163) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,330,4191.T14.3% 138.5 / 25.2 42intergenic (+23/+163)dacB/obgED‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base . (20/16);  new base T (3/3);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02

TTGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGAT  >  NC_000913/3330157‑3330685
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                          
tgtcatgacgctccAGGTCTGGATCGGGCGGGGGTGAATGGAAAAGCCTCAGCGAAAACCGGTTCGTGGCAGGGGGTATATAACCTGGGGGGATTCATTACCACAGCGAGCGGGAAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTc                                                                                                                                                                                                                                                                 <  4:29917/261‑1 (MQ=255)
                     cATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCGTTCTGGCGATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGAGATTTATCAGAACAATTAGGCAAAAAGAAACTCCGGCACAAGGCGGGGGCTTCCGACTATTgcaacaggtgcatg                                                                                                                                                                                                                                           >  4:486320/1‑262 (MQ=255)
                          ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAACGTGCGCTTTGttt                                                                                                                                                                                                                                      >  1:104152/1‑275 (MQ=255)
                                                          ccGGTTCGTTGCAGGGGGGTAATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGGTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                                                                                      <  2:51256/275‑1 (MQ=255)
                                                                                            aTTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                                                                                                                                  >  2:35162/1‑261 (MQ=255)
                                                                                                aTTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                                                                                                                                                              <  4:35161/261‑1 (MQ=255)
                                                                                                  tcccACAGCGGGCGGGCAACGAATGGGCTTTGTGCAAAATCTTTCTGGCTATGACGTAGAGCCGCCGGATCAGCGTAATCGCCGTATTCCGTTAGTTCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTATTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                                                                                                                                                              <  2:486321/273‑1 (MQ=38)
                                                                                                          cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTTCAAATTCAACAGATTGCAATTCTCTTGTAGGCCTg                                                                                                                                                      >  4:292481/1‑275 (MQ=255)
                                                                                                          cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTg                                                                                                                                                      >  2:79370/1‑275 (MQ=255)
                                                                                                                              tttGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                                   <  4:79369/274‑1 (MQ=255)
                                                                                                                                      atatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa                                                                                                                                                                                                            >  4:461027/1‑193 (MQ=255)
                                                                                                                                          cTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                                                                        <  2:461028/193‑1 (MQ=255)
                                                                                                                                                                      aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAAt                                                                                           <  2:181914/274‑1 (MQ=255)
                                                                                                                                                                         aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTGGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGa                                                                                         >  2:105007/1‑273 (MQ=255)
                                                                                                                                                                                    cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                                                                                                                                                                                                    >  7:390993/1‑107 (MQ=255)
                                                                                                                                                                                    cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACaa                                                                                                                                                                                                                                                    <  8:390993/107‑1 (MQ=255)
                                                                                                                                                                                    cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCCATGTGCCgggg                                                                                                                                                                                                                                                                     >  6:393341/1‑90 (MQ=255)
                                                                                                                                                                                    cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCCATGTGCCgggg                                                                                                                                                                                                                                                                     <  5:393341/90‑1 (MQ=255)
                                                                                                                                                                                        ttCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa                                                                                                                                                                                                            >  4:304762/1‑143 (MQ=255)
                                                                                                                                                                                            gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                                                                        <  2:304763/143‑1 (MQ=255)
                                                                                                                                                                                             ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg                                                                                                                                                                                                                                                  >  8:565336/1‑100 (MQ=255)
                                                                                                                                                                                             ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAg                                                                                                                                                                                                                                                  <  7:565336/100‑1 (MQ=255)
                                                                                                                                                                                                gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCtt                                                                 >  4:464143/1‑274 (MQ=255)
                                                                                                                                                                                                     ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACCAAATCGTGCAAATTCAACATATTGCa                                                                                                                                                                       >  1:136361/1‑167 (MQ=255)
                                                                                                                                                                                                     ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATAtt                                                                                                                                                                          >  3:45031/1‑164 (MQ=255)
                                                                                                                                                                                                         gAAATCCGTTTGTATAAAGAAAGTTATCNGAACAATTAGTCAAAAAGACACCCCGGGACAGGGCCGTGGCTGCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGTATGCGGCGTAAACGCCTTATCCGTCCTACAAAATCGTGCAAATTCAACATATTGCAATtc                                                                                                                                                                   <  3:136361/167‑1 (MQ=38)
                                                                                                                                                                                                         gAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa                                                                                                                                                                      <  1:45031/164‑1 (MQ=255)
                                                                                                                                                                                                            aGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          <  5:973244/77‑1 (MQ=255)
                                                                                                                                                                                                            aGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          >  6:973244/1‑77 (MQ=255)
                                                                                                                                                                                                                gTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCCGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCACGTCGTCATc                                                 >  2:500711/1‑274 (MQ=255)
                                                                                                                                                                                                                         aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          >  8:1038465/1‑64 (MQ=255)
                                                                                                                                                                                                                         aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          <  7:1038465/64‑1 (MQ=255)
                                                                                                                                                                                                                              atTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGAGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCatcatc                                  <  1:12814/275‑1 (MQ=255)
                                                                                                                                                                                                                                       aaCAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTc                            >  2:308118/1‑272 (MQ=255)
                                                                                                                                                                                                                                            ttAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAAtc                     >  4:358191/1‑274 (MQ=255)
                                                                                                                                                                                                                                                    aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTAGCATCAGTGATTAACGCTTGTAAATGAACTTAACGCCTTCTTAGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAgc            >  2:509430/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         agccccGGCCATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                                                                                                                                        <  7:367888/48‑1 (MQ=17)
                                                                                                                                                                                                                                                         agccccGGCCATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                                                                                                                                        >  8:367888/3‑50 (MQ=17)
                                                                                                                                                                                                                                                          gccccGGCCATGTGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                                                                                                                                                                                    >  6:969424/2‑69 (MQ=17)
                                                                                                                                                                                                                                                          gccccGGCCATGTGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                                                                                                                                                                                    <  5:969424/68‑1 (MQ=17)
                                                                                                                                                                                                                                                              cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCgat  <  2:464144/275‑1 (MQ=255)
                                                                                                                                                                                                                                                               ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTCGGCCTGATAAGCGTAGCGNATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGNCCCATTCTTCATCCCCTTCTTCATCATCCCNNTCATCCATCTCTTCAAGCTCCCGGCg  >  3:68868/1‑272 (MQ=37)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                          
TTGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGAT  >  NC_000913/3330157‑3330685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: