New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 461476296 (0.610)4 (0.030) 4/258 NT 3.8% coding (782/864 nt) yjjW putative pyruvate formate lyase activating enzyme
?NC_000913 = 4614786 109 (0.710)coding (758/864 nt) yjjW putative pyruvate formate lyase activating enzyme
Rejected: Frequency below/above cutoff threshold.

GAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4614697‑4614762
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgAGGCGCAAAGCTGGGCGAGCGCCACGC  <  NC_000913/4614786‑4614760
                                                                                             
GAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGAGGCGCAAAGCTGGGCGAGCGCCACG   <  5:508796/92‑1
GAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGAGGCGCAAAGCTGGGCGAGCGCCACG   >  6:508796/1‑92
        AACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGAGGCGCAAAGCTGGGCGAGCGCCACGC  <  7:152702/85‑1
        AACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGAGGCGCAAAGCTGGGCGAGCGCCACGC  >  8:152702/1‑85
                                                                                             
GAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4614697‑4614762
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgAGGCGCAAAGCTGGGCGAGCGCCACGC  <  NC_000913/4614786‑4614760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.