Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,441 G→A 5.3% A159T (GCG→ACG) ‡ rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,4410GA 5.3% 243.7 / 3.1 75A159T (GCG→ACG) ‡rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (28/43);  new base A (2/2);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

GCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTG  >  NC_000913/892168‑892705
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                         
gCCATTGCCTTTTATGGGACGGCGGCACTGGGCCAGTTCGGGATGCTGGGGGGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAggc                                                                                                                                                                                                                                                                          <  4:493872/275‑1 (MQ=255)
      gccttgttagggTCGGGGGCACTGGGGCAGGTCGAGGTGCAGGGGAGCTACTCCCGCAAGGAGTCTGCGGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGa                                                                                                                                                                                                                                                                    <  4:15534/266‑1 (MQ=255)
      aCCTTTTATGGGACGGCGGCACTGCGCCAGTTCGCGATGCTGGGGAGCTATCCGCTCAATGCGCCGGTCGCCCTTCCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGCCATCGACCTGCCTGTCCCCGGCATTCCGCATTCGCCGCCTGATACCCGCGCGTCAATCGACCTGCTCGCTGGTTCGCCGCTGGTGGGCACTTTGGTTGAAGTAACGCTGGGTATTAGCCTCGGGCTGGCGGGGTGGCGTCCGGCGGCGGa                                                                                                                                                                                                                                                                    >  4:99768/1‑275 (MQ=255)
       ccTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   <  2:318538/275‑1 (MQ=255)
                                   gTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTTCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCCGGGAATTGGCCGCGTGCTGGCGGGGAGCCGCACGGGGGGGGCAAGCGCGTTTTGCGCCTTCCGCGGTCTGa                                                                                                                                                                                                                                       >  4:54805/1‑275 (MQ=255)
                                     tcggggtggtGGGGAGCTACCCGCCCAAGTGGTCGGGCGCCCTTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATCCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                      <  4:527895/265‑1 (MQ=255)
                                       gggaTGCTGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGGGGGTGGCAAATGGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                    <  2:510761/272‑1 (MQ=255)
                                        ggaTGCGGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcgc                                                                                                                                                                                                                                   <  1:68332/273‑1 (MQ=255)
                                          attcTGGGGCGCTATGCGCTCACGGAGTCGGTCGCCATTGCCCGGGCGCGTGTCAAATTGCGTTACAGTCAACTGCTGGCGCGTCAGGGCATCGCCCTGCCTGGCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTGAATCGACATGGTCGGTGGTGCGCCGCTGGTGGCCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCa                                                                                                                                                                                                                                <  1:66664/272‑1 (MQ=255)
                                                    gCTCTCCGCTAAATGGGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACGGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGAGTTAAGCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                      <  4:445875/275‑1 (MQ=255)
                                                     gtATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGGGCGTGACAAATTGCGTTCCCTGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                      <  4:481030/273‑1 (MQ=255)
                                                      tATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGc                                                                                                                                                                                                                     <  2:54806/274‑1 (MQ=255)
                                                          cgatCAATGGGTCGGGCGCGATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                 <  1:126644/271‑1 (MQ=255)
                                                          cGCTCAATGGGTCGGTTGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAGCTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATGCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGGATTGGCGTCGTGCTGGCGGGGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                 <  2:499272/274‑1 (MQ=255)
                                                            cTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                      <  2:390865/251‑1 (MQ=255)
                                                                                          cgTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCg                                                                                                                                                                                >  2:75296/1‑275 (MQ=255)
                                                                                                        ttCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGCTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGTCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGCATATATCAGAGAGGCGCAAGGGTGCGATTTCCGCTTTCTGcgtgtt                                                                                                                                                                  >  2:523355/1‑274 (MQ=255)
                                                                                                                                 ggCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAggcgg                                                                                                                                                                                                                                                                        >  8:236124/1‑148 (MQ=255)
                                                                                                                                          ctgcctgTCACGGGCATTGCGGATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACggcggg                                                                                                                                <  1:128137/274‑1 (MQ=255)
                                                                                                                                           tgcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGt                                                                                                                                                                                                                                                              >  8:385770/1‑148 (MQ=255)
                                                                                                                                            gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  5:910520/148‑1 (MQ=255)
                                                                                                                                            gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  7:837685/148‑1 (MQ=255)
                                                                                                                                            gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  8:121011/148‑1 (MQ=255)
                                                                                                                                                gTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTg                                                                                                                                                                                                                                                         <  7:385770/148‑1 (MQ=255)
                                                                                                                                                 tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:756932/148‑1 (MQ=255)
                                                                                                                                                 tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  7:236124/148‑1 (MQ=255)
                                                                                                                                                           tGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtttt                                                                                                               >  2:82581/1‑274 (MQ=255)
                                                                                                                                                               cagtggCCGGATGATACAATCGATTTACTCTACATGGTCGGTGGTGGGCATCTGGTGGTCAAGTTGGTTGAAGGACCGCAGGGAATTGGCGCCGTGCGGGCGGAGAGGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTACGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                           <  2:407102/269‑1 (MQ=255)
                                                                                                                                                               cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                  >  6:855218/1‑140 (MQ=255)
                                                                                                                                                               cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                  <  5:855218/140‑1 (MQ=255)
                                                                                                                                                               cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGACCGCGGAAAGCGtca                                                                                                                                                                                                                                                            >  6:537192/1‑128 (MQ=255)
                                                                                                                                                               cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGACCGCGGAAAGCGtca                                                                                                                                                                                                                                                            <  5:537192/130‑3 (MQ=255)
                                                                                                                                                               cATTCGCCGGATGATACCAGCGATTTAATCGACAGGGTGGGGGGGGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGGGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGAAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                           <  2:534321/274‑1 (MQ=255)
                                                                                                                                                                              aCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTNTCCGCGGTCTGAACGCGCCTCTTNTGGGNCAGGCATAGATCAAAGAGGCTCAAGGGTGCGCTATCCGCCTTCTGGTTGTTgg                                                                                                                                                                >  4:539306/1‑206 (MQ=255)
                                                                                                                                                                                 aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        >  6:281725/1‑116 (MQ=255)
                                                                                                                                                                                 aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:281725/116‑1 (MQ=255)
                                                                                                                                                                                  gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   <  8:259593/104‑1 (MQ=255)
                                                                                                                                                                                  gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   >  7:259593/1‑104 (MQ=255)
                                                                                                                                                                                  gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                       <  5:937270/116‑1 (MQ=255)
                                                                                                                                                                                  gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                       >  6:937270/1‑116 (MQ=255)
                                                                                                                                                                                  gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGGGACTTTCGTTCCACTTTGCATCGTggcgg                                                                                        >  4:91051/1‑274 (MQ=255)
                                                                                                                                                                                      tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                                                                                                                                                                                          <  5:300746/110‑5 (MQ=255)
                                                                                                                                                                                      tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                                                                                                                                                                                          >  6:300746/1‑106 (MQ=255)
                                                                                                                                                                                       ttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATCTCACAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTTGCGATGCAGTGGTCGCTGCGACTTAACGGCGGGCCAACGTGGGGCATTTTCCTTCCATTTTCCATCGTtgcggccctgc                                                                                  >  4:132912/1‑270 (MQ=255)
                                                                                                                                                                                       ttAATCGACATGGCCGGCGGTGCGCCGCTGGTGGTCACGTTGGTTGAAGGCACGCCGGGAATTGGCGTCGTTCTGGCGGAGCCGCGTCAGGCCGCCGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGATGCAGGACTATAACAAAGAGGCACAAGGGTTCAGCCACCGTGGCTCGGTTGTTGGCGATGACGTGTGCTCTCTGATTACACGCCGGGGTAATAAGGGACATTTTTGTTCCAAtttcgatgtgagggg                                                                                        >  4:180125/1‑257 (MQ=255)
                                                                                                                                                                                            cGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                             >  8:193288/1‑148 (MQ=255)
                                                                                                                                                                                              aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           <  5:1108976/100‑1 (MQ=255)
                                                                                                                                                                                              aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           >  6:1108976/1‑100 (MQ=255)
                                                                                                                                                                                               cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             >  5:314044/1‑97 (MQ=255)
                                                                                                                                                                                               cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  6:314044/97‑1 (MQ=255)
                                                                                                                                                                                                 gggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGGACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGc                                                                         <  2:16684/273‑1 (MQ=255)
                                                                                                                                                                                                  ggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGCACGAAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGcc                                                                        <  2:422490/274‑1 (MQ=255)
                                                                                                                                                                                                     cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           >  8:1014807/1‑93 (MQ=255)
                                                                                                                                                                                                     cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           <  7:1014807/93‑1 (MQ=255)
                                                                                                                                                                                                      tggggtgCGGCGTTGGTGGGCAAGTTGGTTGAATGAGCGCAGGGAATTGGCGTCGTGCTGGCGTAGACGCGTCATGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATAGCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGAGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                   <  1:57952/272‑1 (MQ=255)
                                                                                                                                                                                                      ggtggtGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                   <  2:99769/275‑1 (MQ=255)
                                                                                                                                                                                                        tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:628157/93‑1 (MQ=255)
                                                                                                                                                                                                        tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        >  6:628157/1‑93 (MQ=255)
                                                                                                                                                                                                                  caggtggtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                 <  7:897677/88‑1 (MQ=255)
                                                                                                                                                                                                                  cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                 >  8:897677/1‑90 (MQ=255)
                                                                                                                                                                                                                         tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCg                                                                                                                                                                                                                                                               <  6:928069/69‑1 (MQ=255)
                                                                                                                                                                                                                         tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCg                                                                                                                                                                                                                                                               >  5:928069/1‑69 (MQ=255)
                                                                                                                                                                                                                         tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                        <  7:356399/124‑1 (MQ=255)
                                                                                                                                                                                                                         tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                        >  8:356399/1‑124 (MQ=255)
                                                                                                                                                                                                                          cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaaa                                                                                                                                                                                                                                                                  <  5:96126/65‑1 (MQ=255)
                                                                                                                                                                                                                          cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaaa                                                                                                                                                                                                                                                                  >  6:96126/1‑65 (MQ=255)
                                                                                                                                                                                                                              ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTc                                                                                                                                                                           <  7:193288/148‑1 (MQ=255)
                                                                                                                                                                                                                                  ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTGTGGTGCAGGAatat                                                                                                                                                                                                             <  8:844384/110‑1 (MQ=255)
                                                                                                                                                                                                                                  ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                             >  7:844384/1‑110 (MQ=255)
                                                                                                                                                                                                                                     aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                               >  8:816066/1‑89 (MQ=255)
                                                                                                                                                                                                                                     aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                               <  7:816066/89‑1 (MQ=255)
                                                                                                                                                                                                                                           cGCAGGTAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAATTGGCGCACGAGTGCGATATCCGCTGGCTGGTTGTTGGCGATGAATTCGCCGCTGCTCTTGACCGGCGGGCGAATTAGGGCGATTTTGGTTCCAATTTTCCTTGTGGCGGTTCTGCTGGCGTCGCCAGTCTCACACNNNATGGCCGTGACATcgccctcaaaacct                                >  3:143273/1‑262 (MQ=255)
                                                                                                                                                                                                                                           cGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCCAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGcc                                 >  2:477572/1‑272 (MQ=255)
                                                                                                                                                                                                                                                  aaTTGGCGTCGGGCTGGCGGAGAAGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                       <  2:539307/275‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaTTGGCGGCGTGCTGGCGGGGACGCGTAAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTTAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                       <  1:151499/275‑1 (MQ=255)
                                                                                                                                                                                                                                                              gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaatg                                                                                                                                                                                                                                    <  6:92923/59‑3 (MQ=255)
                                                                                                                                                                                                                                                              gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                    >  5:92923/1‑59 (MQ=255)
                                                                                                                                                                                                                                                                         aggcttggggcggcGGAAAGCGTGTTTGCCGCTTTCCGGGGTATGAACGCGCATATTCTGGTGCAGGAATATAACAAAGGGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGgtg  <  4:75295/266‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                         
GCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTG  >  NC_000913/892168‑892705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: