Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,443 G→C 6.1% A159A (GCG→GCC) ‡ rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,4430GC 6.1% 257.8 / 5.8 75A159A (GCG→GCC) ‡rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (27/43);  new base C (3/2);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTG  >  NC_000913/892174‑892705
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                       
gccttgttagggTCGGGGGCACTGGGGCAGGTCGAGGTGCAGGGGAGCTACTCCCGCAAGGAGTCTGCGGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGa                                                                                                                                                                                                                                                                    <  4:15534/266‑1 (MQ=255)
aCCTTTTATGGGACGGCGGCACTGCGCCAGTTCGCGATGCTGGGGAGCTATCCGCTCAATGCGCCGGTCGCCCTTCCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGCCATCGACCTGCCTGTCCCCGGCATTCCGCATTCGCCGCCTGATACCCGCGCGTCAATCGACCTGCTCGCTGGTTCGCCGCTGGTGGGCACTTTGGTTGAAGTAACGCTGGGTATTAGCCTCGGGCTGGCGGGGTGGCGTCCGGCGGCGGa                                                                                                                                                                                                                                                                    >  4:99768/1‑275 (MQ=255)
 ccTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   <  2:318538/275‑1 (MQ=255)
                             gTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTTCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCCGGGAATTGGCCGCGTGCTGGCGGGGAGCCGCACGGGGGGGGCAAGCGCGTTTTGCGCCTTCCGCGGTCTGa                                                                                                                                                                                                                                       >  4:54805/1‑275 (MQ=255)
                               tcggggtggtGGGGAGCTACCCGCCCAAGTGGTCGGGCGCCCTTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATCCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                      <  4:527895/265‑1 (MQ=255)
                                 gggaTGCTGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGGGGGTGGCAAATGGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                    <  2:510761/272‑1 (MQ=255)
                                  ggaTGCGGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcgc                                                                                                                                                                                                                                   <  1:68332/273‑1 (MQ=255)
                                    attcTGGGGCGCTATGCGCTCACGGAGTCGGTCGCCATTGCCCGGGCGCGTGTCAAATTGCGTTACAGTCAACTGCTGGCGCGTCAGGGCATCGCCCTGCCTGGCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTGAATCGACATGGTCGGTGGTGCGCCGCTGGTGGCCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCa                                                                                                                                                                                                                                <  1:66664/272‑1 (MQ=255)
                                              gCTCTCCGCTAAATGGGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACGGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGAGTTAAGCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                      <  4:445875/275‑1 (MQ=255)
                                               gtATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGGGCGTGACAAATTGCGTTCCCTGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                      <  4:481030/273‑1 (MQ=255)
                                                tATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGc                                                                                                                                                                                                                     <  2:54806/274‑1 (MQ=255)
                                                    cgatCAATGGGTCGGGCGCGATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                 <  1:126644/271‑1 (MQ=255)
                                                    cGCTCAATGGGTCGGTTGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAGCTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATGCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGGATTGGCGTCGTGCTGGCGGGGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                 <  2:499272/274‑1 (MQ=255)
                                                      cTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                      <  2:390865/251‑1 (MQ=255)
                                                                                    cgTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCg                                                                                                                                                                                >  2:75296/1‑275 (MQ=255)
                                                                                                  ttCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGCTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGTCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGCATATATCAGAGAGGCGCAAGGGTGCGATTTCCGCTTTCTGcgtgtt                                                                                                                                                                  >  2:523355/1‑274 (MQ=255)
                                                                                                                           ggCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAggcgg                                                                                                                                                                                                                                                                        >  8:236124/1‑148 (MQ=255)
                                                                                                                                    ctgcctgTCACGGGCATTGCGGATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACggcggg                                                                                                                                <  1:128137/274‑1 (MQ=255)
                                                                                                                                     tgcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGt                                                                                                                                                                                                                                                              >  8:385770/1‑148 (MQ=255)
                                                                                                                                      gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  7:837685/148‑1 (MQ=255)
                                                                                                                                      gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  5:910520/148‑1 (MQ=255)
                                                                                                                                      gcctgTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  8:121011/148‑1 (MQ=255)
                                                                                                                                          gTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTg                                                                                                                                                                                                                                                         <  7:385770/148‑1 (MQ=255)
                                                                                                                                           tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:756932/148‑1 (MQ=255)
                                                                                                                                           tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  7:236124/148‑1 (MQ=255)
                                                                                                                                                     tGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtttt                                                                                                               >  2:82581/1‑274 (MQ=255)
                                                                                                                                                         cagtggCCGGATGATACAATCGATTTACTCTACATGGTCGGTGGTGGGCATCTGGTGGTCAAGTTGGTTGAAGGACCGCAGGGAATTGGCGCCGTGCGGGCGGAGAGGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTACGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                           <  2:407102/269‑1 (MQ=255)
                                                                                                                                                         cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                  <  5:855218/140‑1 (MQ=255)
                                                                                                                                                         cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                  >  6:855218/1‑140 (MQ=255)
                                                                                                                                                         cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGACCGCGGAAAGCGtca                                                                                                                                                                                                                                                            >  6:537192/1‑128 (MQ=255)
                                                                                                                                                         cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGACCGCGGAAAGCGtca                                                                                                                                                                                                                                                            <  5:537192/130‑3 (MQ=255)
                                                                                                                                                         cATTCGCCGGATGATACCAGCGATTTAATCGACAGGGTGGGGGGGGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGGGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGAAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                           <  2:534321/274‑1 (MQ=255)
                                                                                                                                                                        aCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTNTCCGCGGTCTGAACGCGCCTCTTNTGGGNCAGGCATAGATCAAAGAGGCTCAAGGGTGCGCTATCCGCCTTCTGGTTGTTgg                                                                                                                                                                >  4:539306/1‑206 (MQ=255)
                                                                                                                                                                           aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:281725/116‑1 (MQ=255)
                                                                                                                                                                           aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        >  6:281725/1‑116 (MQ=255)
                                                                                                                                                                            gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   >  7:259593/1‑104 (MQ=255)
                                                                                                                                                                            gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaa                                                                                                                                                                                                                                                                   <  8:259593/104‑1 (MQ=255)
                                                                                                                                                                            gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                       >  6:937270/1‑116 (MQ=255)
                                                                                                                                                                            gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                       <  5:937270/116‑1 (MQ=255)
                                                                                                                                                                            gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGGGACTTTCGTTCCACTTTGCATCGTggcgg                                                                                        >  4:91051/1‑274 (MQ=255)
                                                                                                                                                                                tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                                                                                                                                                                                          <  5:300746/110‑5 (MQ=255)
                                                                                                                                                                                tttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGtcaat                                                                                                                                                                                                                                                          >  6:300746/1‑106 (MQ=255)
                                                                                                                                                                                 ttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATCTCACAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTTGCGATGCAGTGGTCGCTGCGACTTAACGGCGGGCCAACGTGGGGCATTTTCCTTCCATTTTCCATCGTtgcggccctgc                                                                                  >  4:132912/1‑270 (MQ=255)
                                                                                                                                                                                 ttAATCGACATGGCCGGCGGTGCGCCGCTGGTGGTCACGTTGGTTGAAGGCACGCCGGGAATTGGCGTCGTTCTGGCGGAGCCGCGTCAGGCCGCCGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGATGCAGGACTATAACAAAGAGGCACAAGGGTTCAGCCACCGTGGCTCGGTTGTTGGCGATGACGTGTGCTCTCTGATTACACGCCGGGGTAATAAGGGACATTTTTGTTCCAAtttcgatgtgagggg                                                                                        >  4:180125/1‑257 (MQ=255)
                                                                                                                                                                                      cGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                             >  8:193288/1‑148 (MQ=255)
                                                                                                                                                                                        aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           <  5:1108976/100‑1 (MQ=255)
                                                                                                                                                                                        aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           >  6:1108976/1‑100 (MQ=255)
                                                                                                                                                                                         cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             >  5:314044/1‑97 (MQ=255)
                                                                                                                                                                                         cATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTg                                                                                                                                                                                                                                                             <  6:314044/97‑1 (MQ=255)
                                                                                                                                                                                           gggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGGACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGc                                                                         <  2:16684/273‑1 (MQ=255)
                                                                                                                                                                                            ggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGCACGAAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGcc                                                                        <  2:422490/274‑1 (MQ=255)
                                                                                                                                                                                               cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           <  7:1014807/93‑1 (MQ=255)
                                                                                                                                                                                               cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                           >  8:1014807/1‑93 (MQ=255)
                                                                                                                                                                                                tggggtgCGGCGTTGGTGGGCAAGTTGGTTGAATGAGCGCAGGGAATTGGCGTCGTGCTGGCGTAGACGCGTCATGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATAGCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGAGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                   <  1:57952/272‑1 (MQ=255)
                                                                                                                                                                                                ggtggtGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                   <  2:99769/275‑1 (MQ=255)
                                                                                                                                                                                                  tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        <  5:628157/93‑1 (MQ=255)
                                                                                                                                                                                                  tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                        >  6:628157/1‑93 (MQ=255)
                                                                                                                                                                                                            caggtggtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                 <  7:897677/88‑1 (MQ=255)
                                                                                                                                                                                                            cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                 >  8:897677/1‑90 (MQ=255)
                                                                                                                                                                                                                   tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCg                                                                                                                                                                                                                                                               <  6:928069/69‑1 (MQ=255)
                                                                                                                                                                                                                   tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCg                                                                                                                                                                                                                                                               >  5:928069/1‑69 (MQ=255)
                                                                                                                                                                                                                   tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                        <  7:356399/124‑1 (MQ=255)
                                                                                                                                                                                                                   tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                        >  8:356399/1‑124 (MQ=255)
                                                                                                                                                                                                                    cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaaa                                                                                                                                                                                                                                                                  <  5:96126/65‑1 (MQ=255)
                                                                                                                                                                                                                    cAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGaaa                                                                                                                                                                                                                                                                  >  6:96126/1‑65 (MQ=255)
                                                                                                                                                                                                                        ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTc                                                                                                                                                                           <  7:193288/148‑1 (MQ=255)
                                                                                                                                                                                                                            ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTGTGGTGCAGGAatat                                                                                                                                                                                                             <  8:844384/110‑1 (MQ=255)
                                                                                                                                                                                                                            ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                             >  7:844384/1‑110 (MQ=255)
                                                                                                                                                                                                                               aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                               >  8:816066/1‑89 (MQ=255)
                                                                                                                                                                                                                               aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                               <  7:816066/89‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCAGGTAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAATTGGCGCACGAGTGCGATATCCGCTGGCTGGTTGTTGGCGATGAATTCGCCGCTGCTCTTGACCGGCGGGCGAATTAGGGCGATTTTGGTTCCAATTTTCCTTGTGGCGGTTCTGCTGGCGTCGCCAGTCTCACACNNNATGGCCGTGACATcgccctcaaaacct                                >  3:143273/1‑262 (MQ=255)
                                                                                                                                                                                                                                     cGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCCAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGcc                                 >  2:477572/1‑272 (MQ=255)
                                                                                                                                                                                                                                            aaTTGGCGTCGGGCTGGCGGAGAAGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                       <  2:539307/275‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTTGGCGGCGTGCTGGCGGGGACGCGTAAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTTAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                       <  1:151499/275‑1 (MQ=255)
                                                                                                                                                                                                                                                        gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaatg                                                                                                                                                                                                                                    <  6:92923/59‑3 (MQ=255)
                                                                                                                                                                                                                                                        gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                    >  5:92923/1‑59 (MQ=255)
                                                                                                                                                                                                                                                                   aggcttggggcggcGGAAAGCGTGTTTGCCGCTTTCCGGGGTATGAACGCGCATATTCTGGTGCAGGAATATAACAAAGGGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGgtg  <  4:75295/266‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                       
ACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTG  >  NC_000913/892174‑892705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: