Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,457 T→A 14.3% I164N (ATT→AAT)  rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,4570TA14.3% 188.2 / 24.8 70I164N (ATT→AAT) rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base T (23/37);  new base A (5/5);  total (28/42)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02

GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATCG  >  NC_000913/892203‑892727
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                               
gTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTTCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCCGGGAATTGGCCGCGTGCTGGCGGGGAGCCGCACGGGGGGGGCAAGCGCGTTTTGCGCCTTCCGCGGTCTGa                                                                                                                                                                                                                                                             >  4:54805/1‑275 (MQ=255)
  tcggggtggtGGGGAGCTACCCGCCCAAGTGGTCGGGCGCCCTTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATCCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                                            <  4:527895/265‑1 (MQ=255)
    gggaTGCTGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGGGGGTGGCAAATGGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                                          <  2:510761/272‑1 (MQ=255)
     ggaTGCGGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcgc                                                                                                                                                                                                                                                         <  1:68332/273‑1 (MQ=255)
       attcTGGGGCGCTATGCGCTCACGGAGTCGGTCGCCATTGCCCGGGCGCGTGTCAAATTGCGTTACAGTCAACTGCTGGCGCGTCAGGGCATCGCCCTGCCTGGCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTGAATCGACATGGTCGGTGGTGCGCCGCTGGTGGCCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCa                                                                                                                                                                                                                                                      <  1:66664/272‑1 (MQ=255)
                 gCTCTCCGCTAAATGGGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACGGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGAGTTAAGCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                                            <  4:445875/275‑1 (MQ=255)
                  gtATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGGGCGTGACAAATTGCGTTCCCTGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                                                                                                                                            <  4:481030/273‑1 (MQ=255)
                   tATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGc                                                                                                                                                                                                                                           <  2:54806/274‑1 (MQ=255)
                       cgatCAATGGGTCGGGCGCGATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                                       <  1:126644/271‑1 (MQ=255)
                       cGCTCAATGGGTCGGTTGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAGCTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATGCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGGATTGGCGTCGTGCTGGCGGGGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa                                                                                                                                                                                                                                       <  2:499272/274‑1 (MQ=255)
                         cTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa                                                                                                                                                                                                                                                            <  2:390865/251‑1 (MQ=255)
                                                       cgTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCg                                                                                                                                                                                                      >  2:75296/1‑275 (MQ=255)
                                                                     ttCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGCTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGTCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGCATATATCAGAGAGGCGCAAGGGTGCGATTTCCGCTTTCTGcgtgtt                                                                                                                                                                                        >  2:523355/1‑274 (MQ=255)
                                                                                                       ctgcctgTCACGGGCATTGCGGATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACggcggg                                                                                                                                                      <  1:128137/274‑1 (MQ=255)
                                                                                                             gTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTg                                                                                                                                                                                                                                                                               <  7:385770/148‑1 (MQ=255)
                                                                                                              tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              <  7:236124/148‑1 (MQ=255)
                                                                                                              tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              <  5:756932/148‑1 (MQ=255)
                                                                                                                        tGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtttt                                                                                                                                     >  2:82581/1‑274 (MQ=255)
                                                                                                                            cagtggCCGGATGATACAATCGATTTACTCTACATGGTCGGTGGTGGGCATCTGGTGGTCAAGTTGGTTGAAGGACCGCAGGGAATTGGCGCCGTGCGGGCGGAGAGGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTACGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                                                 <  2:407102/269‑1 (MQ=255)
                                                                                                                            cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                                        >  6:855218/1‑140 (MQ=255)
                                                                                                                            cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt                                                                                                                                                                                                                                                                        <  5:855218/140‑1 (MQ=255)
                                                                                                                            cATTCGCCGGATGATACCAGCGATTTAATCGACAGGGTGGGGGGGGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGGGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGAAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt                                                                                                                                 <  2:534321/274‑1 (MQ=255)
                                                                                                                                           aCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTNTCCGCGGTCTGAACGCGCCTCTTNTGGGNCAGGCATAGATCAAAGAGGCTCAAGGGTGCGCTATCCGCCTTCTGGTTGTTgg                                                                                                                                                                                      >  4:539306/1‑206 (MQ=255)
                                                                                                                                              aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              <  5:281725/116‑1 (MQ=255)
                                                                                                                                              aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              >  6:281725/1‑116 (MQ=255)
                                                                                                                                               gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                                             <  5:937270/116‑1 (MQ=255)
                                                                                                                                               gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc                                                                                                                                                                                                                                                                             >  6:937270/1‑116 (MQ=255)
                                                                                                                                               gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGGGACTTTCGTTCCACTTTGCATCGTggcgg                                                                                                              >  4:91051/1‑274 (MQ=255)
                                                                                                                                                    ttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATCTCACAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTTGCGATGCAGTGGTCGCTGCGACTTAACGGCGGGCCAACGTGGGGCATTTTCCTTCCATTTTCCATCGTtgcggccctgc                                                                                                        >  4:132912/1‑270 (MQ=255)
                                                                                                                                                    ttAATCGACATGGCCGGCGGTGCGCCGCTGGTGGTCACGTTGGTTGAAGGCACGCCGGGAATTGGCGTCGTTCTGGCGGAGCCGCGTCAGGCCGCCGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGATGCAGGACTATAACAAAGAGGCACAAGGGTTCAGCCACCGTGGCTCGGTTGTTGGCGATGACGTGTGCTCTCTGATTACACGCCGGGGTAATAAGGGACATTTTTGTTCCAAtttcgatgtgagggg                                                                                                              >  4:180125/1‑257 (MQ=255)
                                                                                                                                                         cGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                                                   >  8:193288/1‑148 (MQ=255)
                                                                                                                                                           aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                                                 <  5:1108976/100‑1 (MQ=255)
                                                                                                                                                           aCATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                                                 >  6:1108976/1‑100 (MQ=255)
                                                                                                                                                              gggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGGACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGc                                                                                               <  2:16684/273‑1 (MQ=255)
                                                                                                                                                               ggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGCACGAAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGcc                                                                                              <  2:422490/274‑1 (MQ=255)
                                                                                                                                                                  cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                                                 <  7:1014807/93‑1 (MQ=255)
                                                                                                                                                                  cGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGAt                                                                                                                                                                                                                                                                                 >  8:1014807/1‑93 (MQ=255)
                                                                                                                                                                   tggggtgCGGCGTTGGTGGGCAAGTTGGTTGAATGAGCGCAGGGAATTGGCGTCGTGCTGGCGTAGACGCGTCATGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATAGCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGAGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                                         <  1:57952/272‑1 (MQ=255)
                                                                                                                                                                   ggtggtGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt                                                                                         <  2:99769/275‑1 (MQ=255)
                                                                                                                                                                     tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              >  6:628157/1‑93 (MQ=255)
                                                                                                                                                                     tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa                                                                                                                                                                                                                                                                              <  5:628157/93‑1 (MQ=255)
                                                                                                                                                                               caggtggtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                                       <  7:897677/88‑1 (MQ=255)
                                                                                                                                                                               cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                                                                                                                                                       >  8:897677/1‑90 (MQ=255)
                                                                                                                                                                                      tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                                              >  8:356399/1‑124 (MQ=255)
                                                                                                                                                                                      tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa                                                                                                                                                                                                                              <  7:356399/124‑1 (MQ=255)
                                                                                                                                                                                           ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTc                                                                                                                                                                                                 <  7:193288/148‑1 (MQ=255)
                                                                                                                                                                                               ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTGTGGTGCAGGAatat                                                                                                                                                                                                                                   <  8:844384/110‑1 (MQ=255)
                                                                                                                                                                                               ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat                                                                                                                                                                                                                                   >  7:844384/1‑110 (MQ=255)
                                                                                                                                                                                                  aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                                                     >  8:816066/1‑89 (MQ=255)
                                                                                                                                                                                                  aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat                                                                                                                                                                                                                                                     <  7:816066/89‑1 (MQ=255)
                                                                                                                                                                                                        cGCAGGTAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAATTGGCGCACGAGTGCGATATCCGCTGGCTGGTTGTTGGCGATGAATTCGCCGCTGCTCTTGACCGGCGGGCGAATTAGGGCGATTTTGGTTCCAATTTTCCTTGTGGCGGTTCTGCTGGCGTCGCCAGTCTCACACNNNATGGCCGTGACATcgccctcaaaacct                                                      >  3:143273/1‑262 (MQ=255)
                                                                                                                                                                                                        cGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCCAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGcc                                                       >  2:477572/1‑272 (MQ=255)
                                                                                                                                                                                                               aaTTGGCGTCGGGCTGGCGGAGAAGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                                             <  2:539307/275‑1 (MQ=255)
                                                                                                                                                                                                               aaTTGGCGGCGTGCTGGCGGGGACGCGTAAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTTAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa                                             <  1:151499/275‑1 (MQ=255)
                                                                                                                                                                                                                           gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaatg                                                                                                                                                                                                                                                          <  6:92923/59‑3 (MQ=255)
                                                                                                                                                                                                                           gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                                                                                                                                          >  5:92923/1‑59 (MQ=255)
                                                                                                                                                                                                                                     aggcttggggcggcGGAAAGCGTGTTTGCCGCTTTCCGGGGTATGAACGCGCATATTCTGGTGCAGGAATATAACAAAGGGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGgtg                        <  4:75295/266‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCa                                                                                                                                                                                                                                          >  6:614418/1‑49 (MQ=255)
                                                                                                                                                                                                                                                     aaaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCa                                                                                                                                                                                                                                          <  5:614418/49‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGaa                                                                                                                                                                                                                                      <  5:1074299/53‑1 (MQ=25)
                                                                                                                                                                                                                                                     aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGaa                                                                                                                                                                                                                                      >  6:1074299/1‑53 (MQ=25)
                                                                                                                                                                                                                                                     aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCg                                                                                                                                                                   <  7:560506/119‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCg                                                                                                                                                                   >  8:560506/1‑119 (MQ=255)
                                                                                                                                                                                                                                                      aaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTCTTGGCg                                                                                                                                                                                    <  6:605795/101‑1 (MQ=255)
                                                                                                                                                                                                                                                      aaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGCTGTTGGCg                                                                                                                                                                                    >  5:605795/1‑101 (MQ=255)
                                                                                                                                                                                                                                                      aaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAagag                                                                                                                                              >  7:63655/1‑139 (MQ=255)
                                                                                                                                                                                                                                                      aaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAagag                                                                                                                                              <  8:63655/139‑1 (MQ=255)
                                                                                                                                                                                                                                                       aGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATcc                                                                                                                                                                                                       <  7:460141/82‑1 (MQ=39)
                                                                                                                                                                                                                                                       aGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATcc                                                                                                                                                                                                       >  8:460141/1‑82 (MQ=39)
                                                                                                                                                                                                                                                          gTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGa                                                                                                                                                              <  7:38/119‑1 (MQ=255)
                                                                                                                                                                                                                                                          gTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGa                                                                                                                                                              >  8:38/1‑119 (MQ=255)
                                                                                                                                                                                                                                                             aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTgg                                                                                                                                                                                      <  6:627173/92‑1 (MQ=255)
                                                                                                                                                                                                                                                             aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTgg                                                                                                                                                                                      >  5:627173/1‑92 (MQ=255)
                                                                                                                                                                                                                                                             aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATcg  <  2:132913/272‑1 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                               
GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATCG  >  NC_000913/892203‑892727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: