| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 892,459 | G→C | 13.9% | D165H (GAC→CAC) | rimK → | ribosomal protein S6 modification protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 892,459 | 0 | G | C | 13.9% | 219.8 / 25.5 | 73 | D165H (GAC→CAC) | rimK | ribosomal protein S6 modification protein |
| Reads supporting (aligned to +/- strand): ref base G (23/37); new base C (5/5); total (30/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-02 | |||||||||||
GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATCG > NC_000913/892203‑892727 | gTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTTCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCCGGGAATTGGCCGCGTGCTGGCGGGGAGCCGCACGGGGGGGGCAAGCGCGTTTTGCGCCTTCCGCGGTCTGa > 4:54805/1‑275 (MQ=255) tcggggtggtGGGGAGCTACCCGCCCAAGTGGTCGGGCGCCCTTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATCCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa < 4:527895/265‑1 (MQ=255) gggaTGCTGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGGGGGTGGCAAATGGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg < 2:510761/272‑1 (MQ=255) ggaTGCGGGTGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcgc < 1:68332/273‑1 (MQ=255) attcTGGGGCGCTATGCGCTCACGGAGTCGGTCGCCATTGCCCGGGCGCGTGTCAAATTGCGTTACAGTCAACTGCTGGCGCGTCAGGGCATCGCCCTGCCTGGCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTGAATCGACATGGTCGGTGGTGCGCCGCTGGTGGCCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCa < 1:66664/272‑1 (MQ=255) gCTCTCCGCTAAATGGGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACGGCGGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGAGTTAAGCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg < 4:445875/275‑1 (MQ=255) gtATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGGGCGTGACAAATTGCGTTCCCTGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg < 4:481030/273‑1 (MQ=255) tATCCGCTCAATGGGTCGGTCGCCATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGc < 2:54806/274‑1 (MQ=255) cgatCAATGGGTCGGGCGCGATTGCCCGGGCGCGGGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa < 1:126644/271‑1 (MQ=255) cGCTCAATGGGTCGGTTGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAGCTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATGCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGGATTGGCGTCGTGCTGGCGGGGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGa < 2:499272/274‑1 (MQ=255) cTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaa < 2:390865/251‑1 (MQ=255) cgTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCg > 2:75296/1‑275 (MQ=255) ttCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGCTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGTCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGCATATATCAGAGAGGCGCAAGGGTGCGATTTCCGCTTTCT‑Gcgtgtt > 2:523355/1‑274 (MQ=255) ctgcctgTCACGGGCATTGCGGATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACggcggg < 1:128137/274‑1 (MQ=255) gTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTg < 7:385770/148‑1 (MQ=255) tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa < 5:756932/148‑1 (MQ=255) tCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa < 7:236124/148‑1 (MQ=255) tGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtttt > 2:82581/1‑274 (MQ=255) cagtggCCGGATGATACAATCGATTTACTCTACATGGTCGGTGGTGGGCATCTGGTGGTCAAGTTGGTTGAAGGACCGCAGGGAATTGGCGCCGTGCGGGCGGAGAGGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTACGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt < 2:407102/269‑1 (MQ=255) cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt < 5:855218/140‑1 (MQ=255) cATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCttt > 6:855218/1‑140 (MQ=255) cATTCGCCGGATGATACCAGCGATTTAATCGACAGGGTGGGGGGGGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGGGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGAAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt < 2:534321/274‑1 (MQ=255) aCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTNTCCGCGGTCTGAACGCGCCTCTTNTGGGNCAGGCATAGATCAAAGAGGCTCAAGGGTGCGCTATCCGCCTTCT‑GGTTGTTgg > 4:539306/1‑206 (MQ=255) aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa > 6:281725/1‑116 (MQ=255) aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa < 5:281725/116‑1 (MQ=255) gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc < 5:937270/116‑1 (MQ=255) gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGAc > 6:937270/1‑116 (MQ=255) gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGGGACTTTCGTTCCACTTTGCATCGTggcgg > 4:91051/1‑274 (MQ=255) ttAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATCTCACAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTTGCGATGCAGTGGTCGCTGCGACTTAACGGCGGGCCAACGTGGGGCATTTTCCTTCCATTTTCCATCGTtgcggccctgc > 4:132912/1‑270 (MQ=255) ttAATCGACATGGCCGGCGGTGCGCCGCTGGTGGTCACGTTGGTTGAAGGCACGCCGGGAATTGGCGTCGTTCTGGCGGAGCCGCGTCAGGCCGCCGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGATGCAGGACTATAACAAAGAGGCACAAGGGTTCAGCCACCG‑TGGCTCGGTTGTTGGCGATGACGTGTGCTCTCTGATTACACGCCGGGGTAATAAGGGACATTTTTGTTCCAAtttcgatgtgagggg > 4:180125/1‑257 (MQ=255) cGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat > 8:193288/1‑148 (MQ=255) gggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGGACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGc < 2:16684/273‑1 (MQ=255) ggTCGGTGGTGCGCCGGTGGTGGTCAAGTTGGTTGAAGGCACGAAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGcc < 2:422490/274‑1 (MQ=255) tggggtgCGGCGTTGGTGGGCAAGTTGGTTGAATGAGCGCAGGGAATTGGCGTCGTGCTGGCGTAGACGCGTCATGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATAGCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGAGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt < 1:57952/272‑1 (MQ=255) ggtggtGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTAt < 2:99769/275‑1 (MQ=255) tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa > 6:628157/1‑93 (MQ=255) tggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGa < 5:628157/93‑1 (MQ=255) caggtggtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc < 7:897677/88‑1 (MQ=255) cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc > 8:897677/1‑90 (MQ=255) tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa > 8:356399/1‑124 (MQ=255) tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCaa < 7:356399/124‑1 (MQ=255) ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTc < 7:193288/148‑1 (MQ=255) ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTGTGGTGCAGGAatat < 8:844384/110‑1 (MQ=255) ttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAatat > 7:844384/1‑110 (MQ=255) aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat > 8:816066/1‑89 (MQ=255) aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCat < 7:816066/89‑1 (MQ=255) cGCAGGTAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAATTGGCGCACGAGTGCGATATCCGCTGGCT‑GGTTGTTGGCGATGAATTCGCCGCTGCTCTTGACCGGCGGGCGAATTAGGGCGATTTTGGTTCCAATTTTCCTTGTGGCGGTTCTGCTGGCGTCGCCAGTCTCACACNNNATGGCCGTGACATcgccctcaaaacct > 3:143273/1‑262 (MQ=255) cGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGACCGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCCAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGcc > 2:477572/1‑272 (MQ=255) aaTTGGCGTCGGGCTGGCGGAGAAGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTGCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa < 2:539307/275‑1 (MQ=255) aaTTGGCGGCGTGCTGGCGGGGACGCGTAAGGCGGCGGAAAGCGTGATTTACGCTTTCCGCGGTCTTAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGa < 1:151499/275‑1 (MQ=255) gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGaatg < 6:92923/59‑3 (MQ=255) gCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg > 5:92923/1‑59 (MQ=255) aggcttggggcggcGGAAAGCGTGTTTGCCGCTTTCCGGGGTATGAACGCGCATATTCTGGTGCAGGAATATAACAAAGGGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGgtg < 4:75295/266‑1 (MQ=255) aaaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCa < 5:614418/49‑1 (MQ=255) aaaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCa > 6:614418/1‑49 (MQ=255) aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGaa < 5:1074299/53‑1 (MQ=25) aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGaa > 6:1074299/1‑53 (MQ=25) aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCg > 8:560506/1‑119 (MQ=255) aaaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCg < 7:560506/119‑1 (MQ=255) aaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTCTTGGCg < 6:605795/101‑1 (MQ=255) aaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGCTGTTGGCg > 5:605795/1‑101 (MQ=255) aaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAagag < 8:63655/139‑1 (MQ=255) aaGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAagag > 7:63655/1‑139 (MQ=255) aGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATcc > 8:460141/1‑82 (MQ=39) aGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATcc < 7:460141/82‑1 (MQ=39) gTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGa > 8:38/1‑119 (MQ=255) gTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGa < 7:38/119‑1 (MQ=255) aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTgg < 6:627173/92‑1 (MQ=255) aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTgg > 5:627173/1‑92 (MQ=255) aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATcg < 2:132913/272‑1 (MQ=255) tGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑Ggttgt > 7:495482/1‑87 (MQ=255) tGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑Ggttgt < 8:495482/87‑1 (MQ=255) tGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCg < 8:1107877/109‑1 (MQ=255) tGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCg > 7:1107877/1‑109 (MQ=255) | GTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGACAAATTGCGTTCCATGCAACTGCTGGCGCGTCAGGGCATCGACCTGCCTGTCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCT‑GGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTGCATCGTGGCGGCGCGGCAAGCGTCGCCAGTATCACACCACAGGAGCGTGAAATCGCGATAAAAGCCGCGCGAACGATGGCGCTGGACGTTGCTGGTGTGGATATTCTGCGTGCTAATCG > NC_000913/892203‑892727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |