| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 915,081 | G→C | 6.4% | intergenic (‑224/+271) | ybjE ← / ← aqpZ | putative transporter/aquaporin Z |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 915,081 | 0 | G | C | 6.4% | 201.8 / 5.9 | 63 | intergenic (‑224/+271) | ybjE/aqpZ | putative transporter/aquaporin Z |
| Reads supporting (aligned to +/- strand): ref base G (31/28); new base C (2/2); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-02 | |||||||||||
TAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCG > NC_000913/914811‑915347 | tAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAGAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGGTCCTTGAAGGCCGGGGCAGCCCACATGGAtg > 2:466380/1‑274 (MQ=255) caATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACTTGGATGTGGGCTGa > 2:346439/1‑275 (MQ=255) aCCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTTGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGCCCCGttt > 4:236013/1‑274 (MQ=255) aCCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGCCGAAATGCGCGATGACACACTACTGAATGCGGAAGGACGAGGAAAGTCGCAATTAAAGGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCATGCGCTTT‑AATTACAACATTCACCAATGTTGCTGGC‑GTCAAACTCGCCGAGCCGTTCCCGTAAGGCCGGGGCCGCCCACTTGGGTTTCGGACGAGGCCGCGttt > 4:488724/1‑274 (MQ=255) taaTTATTAACAGCCCAGGAAACATGGAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTTCTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGGTGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGggcg < 2:488725/266‑1 (MQ=255) gAATGATTAACAGCCCAAAAAACAGGAAAACCCAGGCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCAGAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTGCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAg < 4:346438/275‑1 (MQ=255) aaCCCATTCCTTGCAAAGATGAATGCGAAATGCACGATGACACACTACTGAAAGCGGAAAGACAAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCTCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt < 2:179047/273‑1 (MQ=255) aaCCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAGGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg > 2:115990/1‑226 (MQ=255) cAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCt < 4:115989/226‑1 (MQ=255) aTGAAGTCGAAATGCGGGATGACAAACTACTGAAAGAGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGGTATTCTTGCGCTTT‑AATAACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTAGa < 2:7489/275‑1 (MQ=255) cacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg > 2:120904/1‑275 (MQ=255) ctGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGc > 2:250825/1‑275 (MQ=255) aaaGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAACTGTTATGCATAAGGAGCAGCAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGTATGTTACCTCGCGCCCGACCCGGTATCCGTCAGGGATAAGTCGAGGCCACCGCTCAGCGGCTTTTTTGTTCGCCag > 2:255336/1‑275 (MQ=255) aaaGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCACAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACATCGCGCCCGACACGGTAGCCGTAAGGGATAAGTCGCGGGCACCGAGCAGCGGCGATTTTGTTCGCCag > 1:48707/1‑275 (MQ=255) aaGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgg > 1:99132/1‑275 (MQ=255) gTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGgcg > 4:50148/1‑167 (MQ=255) gTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc > 2:229480/1‑275 (MQ=255) cAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtt < 2:50149/167‑1 (MQ=255) aaaTGTTTGGCATAAGGAGAAGGTTGGTATTGGTTTTCATTTAAAGAAATTGTTCGGCTTT‑ATTTAAAAACTGCAGCAAGGTTGGTGGC‑GTAAAAAGCGCCGGGGGGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCCCGCGCCGGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgc < 4:533910/273‑1 (MQ=14) tGCACAAGGAGAAGTAGAGAATTCATTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAACTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTCAGGGCGCGtt < 8:163525/148‑1 (MQ=25) gCATAAGGGGCAGTGGTGTATACGTTTTATATTAAAGATATTCTTGCGCTTT‑AATTACAAACTGAACAGATGTTGTGGTG‑GTCAAAATTGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACAGGGATGGGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg < 4:229479/275‑1 (MQ=14) aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcgc > 2:102536/1‑274 (MQ=255) aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc > 8:862925/1‑131 (MQ=255) aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc < 7:862925/131‑1 (MQ=255) gtgTATTAGGTTTGATTTAAAGGAATGCTTGCGCTTT‑AATGACAAACTGGACGGAGGTTGGTGGG‑GTCAAAAGCGCCGAGGCGTTCCCGCAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTGTTAAAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGAACCGCGCAGCGGCGATTTTGTTCGCCAGAGCACGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg < 4:137994/273‑1 (MQ=14) tgaaTTCGTTTATATTTAAAGTGATCCTT‑CGCTTTGAATTACAAAGTGGCCCGA‑GGTGGTGGCGGTAAAACGCGCCGGGGCGGTCCCTGAAGGCCGGGGCAGCGCACATGGATGGGGGCTGAGGGCGCGTTTTACAGGGAGGTTACCGCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg < 4:15691/271‑1 (MQ=11) tATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTgg > 4:95802/1‑275 (MQ=255) aTTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg < 5:279521/113‑1 (MQ=255) aTTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg > 6:279521/1‑113 (MQ=255) ttCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAg > 2:12128/1‑168 (MQ=255) gTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAt > 4:400835/1‑196 (MQ=255) ttttCATTTAAAGATATTTTGGCGCTTT‑AATGACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTTAAGGCCGGGGCAGCCCCCATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt < 4:12128/168‑1 (MQ=255) tCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg < 2:400836/196‑1 (MQ=255) cATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTAGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg > 4:309920/1‑275 (MQ=255) aaaGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg < 2:95803/275‑1 (MQ=255) aGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGc < 5:561242/100‑1 (MQ=255) aGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGc > 6:561242/1‑100 (MQ=255) gATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg > 8:1115121/1‑96 (MQ=255) gATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg < 7:1115121/96‑1 (MQ=255) cgcTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTAGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg < 2:309921/275‑1 (MQ=255) cgcATT‑AATTAGAAACTGCGCCGAGGGTGGGGGC‑GTAAAAATCGCCAAGGCGGTCCCTGAAGGCCGGGGGGGCCCACATGGATGTGGGTGGGAGGCGCGTTTTACAGGGATGGTACCTCGCGCCGGACCGGTAAGCCGTAAGGGATAAGTCGGGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg < 4:120903/275‑1 (MQ=17) tACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa > 7:636019/1‑97 (MQ=255) tACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa < 8:636019/97‑1 (MQ=255) cAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg > 7:299889/1‑75 (MQ=255) cAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg < 8:299889/75‑1 (MQ=255) aaCTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg > 5:777094/1‑80 (MQ=255) aaCTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg < 6:777094/80‑1 (MQ=255) aCTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAgg > 8:32590/1‑129 (MQ=255) aCTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAgg < 7:32590/129‑1 (MQ=255) cACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGGGCGCAACAAGATGATGaa > 2:447148/1‑274 (MQ=255) cACCGATGTTGGGGGC‑GTCAAAATCGCCGGGGGGTCCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGGGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt < 4:250824/275‑1 (MQ=255) aTGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTTCGCCAGTGGCAATATGTTGCTTAGCCCATGGAAGGAGCGCAAAACAATAGTGAATCcaca > 4:471191/1‑275 (MQ=14) ggtggC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCCCCTTGCGCCAGTGGCAATATGTTCCCTAGCTCACGAAGGGAGCGCAAAAAGATGATCAATCAACATaaaa > 4:291731/1‑275 (MQ=255) c‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAccc > 6:22685/1‑97 (MQ=34) c‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAccc < 5:22685/97‑1 (MQ=34) c‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaacaac > 4:155338/1‑274 (MQ=255) tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtttt < 8:144657/62‑1 (MQ=33) tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtttt > 7:144657/1‑62 (MQ=33) tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt < 6:418949/72‑1 (MQ=32) tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt > 5:418949/1‑72 (MQ=32) gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTc < 8:1102144/80‑1 (MQ=255) gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTc > 7:1102144/1‑80 (MQ=255) ccACATGGAGGTGGGCTGTGGGCGCGTTGTACAGGGATGGTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg < 2:236014/274‑1 (MQ=255) | TAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTT‑AATTACAAACTGCACCGATGTTGGTGGC‑GTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCG > NC_000913/914811‑915347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |