Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,081 G→C 6.4% intergenic (‑224/+271) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,0810GC 6.4% 201.8 / 5.9 63intergenic (‑224/+271)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base C (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-02

TAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCG  >  NC_000913/914811‑915347
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                          
tAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAGAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGGTCCTTGAAGGCCGGGGCAGCCCACATGGAtg                                                                                                                                                                                                                                                                         >  2:466380/1‑274 (MQ=255)
       caATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACTTGGATGTGGGCTGa                                                                                                                                                                                                                                                                 >  2:346439/1‑275 (MQ=255)
                  aCCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTTGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGCCCCGttt                                                                                                                                                                                                                                                       >  4:236013/1‑274 (MQ=255)
                  aCCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGCCGAAATGCGCGATGACACACTACTGAATGCGGAAGGACGAGGAAAGTCGCAATTAAAGGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCATGCGCTTTAATTACAACATTCACCAATGTTGCTGGCGTCAAACTCGCCGAGCCGTTCCCGTAAGGCCGGGGCCGCCCACTTGGGTTTCGGACGAGGCCGCGttt                                                                                                                                                                                                                                                       >  4:488724/1‑274 (MQ=255)
                      taaTTATTAACAGCCCAGGAAACATGGAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTTCTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGGTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGggcg                                                                                                                                                                                                                                                          <  2:488725/266‑1 (MQ=255)
                      gAATGATTAACAGCCCAAAAAACAGGAAAACCCAGGCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCAGAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTGCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                                                                                                                                                                  <  4:346438/275‑1 (MQ=255)
                                                  aaCCCATTCCTTGCAAAGATGAATGCGAAATGCACGATGACACACTACTGAAAGCGGAAAGACAAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCTCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                                                                                                                                                                        <  2:179047/273‑1 (MQ=255)
                                                  aaCCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAGGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       >  2:115990/1‑226 (MQ=255)
                                                      cAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCt                                                                                                                                                                                                                                                                   <  4:115989/226‑1 (MQ=255)
                                                                    aTGAAGTCGAAATGCGGGATGACAAACTACTGAAAGAGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGGTATTCTTGCGCTTTAATAACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTAGa                                                                                                                                                                                                    <  2:7489/275‑1 (MQ=255)
                                                                                            cacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg                                                                                                                                                                            >  2:120904/1‑275 (MQ=255)
                                                                                                 ctGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGc                                                                                                                                                                       >  2:250825/1‑275 (MQ=255)
                                                                                                    aaaGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAACTGTTATGCATAAGGAGCAGCAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGTATGTTACCTCGCGCCCGACCCGGTATCCGTCAGGGATAAGTCGAGGCCACCGCTCAGCGGCTTTTTTGTTCGCCag                                                                                                                                                                    >  2:255336/1‑275 (MQ=255)
                                                                                                    aaaGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCACAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACATCGCGCCCGACACGGTAGCCGTAAGGGATAAGTCGCGGGCACCGAGCAGCGGCGATTTTGTTCGCCag                                                                                                                                                                    >  1:48707/1‑275 (MQ=255)
                                                                                                           aaGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgg                                                                                                                                                             >  1:99132/1‑275 (MQ=255)
                                                                                                                        gTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGgcg                                                                                                                                                                                                                                                            >  4:50148/1‑167 (MQ=255)
                                                                                                                        gTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc                                                                                                                                                >  2:229480/1‑275 (MQ=255)
                                                                                                                            cAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtt                                                                                                                                                                                                                                                        <  2:50149/167‑1 (MQ=255)
                                                                                                                                       aaaTGTTTGGCATAAGGAGAAGGTTGGTATTGGTTTTCATTTAAAGAAATTGTTCGGCTTTATTTAAAAACTGCAGCAAGGTTGGTGGCGTAAAAAGCGCCGGGGGGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCCCGCGCCGGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGgc                                                                                                                                   <  4:533910/273‑1 (MQ=14)
                                                                                                                                               tGCACAAGGAGAAGTAGAGAATTCATTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAACTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTCAGGGCGCGtt                                                                                                                                                                                                                                                        <  8:163525/148‑1 (MQ=25)
                                                                                                                                                gCATAAGGGGCAGTGGTGTATACGTTTTATATTAAAGATATTCTTGCGCTTTAATTACAAACTGAACAGATGTTGTGGTGGTCAAAATTGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACAGGGATGGGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                        <  4:229479/275‑1 (MQ=14)
                                                                                                                                                    aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcgc                                                                                                                     >  2:102536/1‑274 (MQ=255)
                                                                                                                                                    aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                    >  8:862925/1‑131 (MQ=255)
                                                                                                                                                    aaGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATCCATGTGGGc                                                                                                                                                                                                                                                                    <  7:862925/131‑1 (MQ=255)
                                                                                                                                                               gtgTATTAGGTTTGATTTAAAGGAATGCTTGCGCTTTAATGACAAACTGGACGGAGGTTGGTGGGGTCAAAAGCGCCGAGGCGTTCCCGCAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTGTTAAAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGAACCGCGCAGCGGCGATTTTGTTCGCCAGAGCACGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                         <  4:137994/273‑1 (MQ=14)
                                                                                                                                                                tgaaTTCGTTTATATTTAAAGTGATCCTTCGCTTTGAATTACAAAGTGGCCCGAGGTGGTGGCGGTAAAACGCGCCGGGGCGGTCCCTGAAGGCCGGGGCAGCGCACATGGATGGGGGCTGAGGGCGCGTTTTACAGGGAGGTTACCGCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                         <  4:15691/271‑1 (MQ=11)
                                                                                                                                                                  tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTgg                                                                                                      >  4:95802/1‑275 (MQ=255)
                                                                                                                                                                   aTTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       <  5:279521/113‑1 (MQ=255)
                                                                                                                                                                   aTTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       >  6:279521/1‑113 (MQ=255)
                                                                                                                                                                    ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAg                                                                                                                                                                                                               >  2:12128/1‑168 (MQ=255)
                                                                                                                                                                       gTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAt                                                                                                                                                                                >  4:400835/1‑196 (MQ=255)
                                                                                                                                                                        ttttCATTTAAAGATATTTTGGCGCTTTAATGACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTTAAGGCCGGGGCAGCCCCCATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                                                                                                                                                                           <  4:12128/168‑1 (MQ=255)
                                                                                                                                                                           tCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTg                                                                                                                                                                            <  2:400836/196‑1 (MQ=255)
                                                                                                                                                                            cATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTAGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                            >  4:309920/1‑275 (MQ=255)
                                                                                                                                                                                 aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                       <  2:95803/275‑1 (MQ=255)
                                                                                                                                                                                   aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGc                                                                                                                                                                                                                                                                    <  5:561242/100‑1 (MQ=255)
                                                                                                                                                                                   aGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGc                                                                                                                                                                                                                                                                    >  6:561242/1‑100 (MQ=255)
                                                                                                                                                                                    gATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       >  8:1115121/1‑96 (MQ=255)
                                                                                                                                                                                    gATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       <  7:1115121/96‑1 (MQ=255)
                                                                                                                                                                                              cgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTAGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                          <  2:309921/275‑1 (MQ=255)
                                                                                                                                                                                              cgcATTAATTAGAAACTGCGCCGAGGGTGGGGGCGTAAAAATCGCCAAGGCGGTCCCTGAAGGCCGGGGGGGCCCACATGGATGTGGGTGGGAGGCGCGTTTTACAGGGATGGTACCTCGCGCCGGACCGGTAAGCCGTAAGGGATAAGTCGGGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAg                                                                          <  4:120903/275‑1 (MQ=17)
                                                                                                                                                                                                        tACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa                                                                                                                                                                                                                                                   >  7:636019/1‑97 (MQ=255)
                                                                                                                                                                                                        tACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACa                                                                                                                                                                                                                                                   <  8:636019/97‑1 (MQ=255)
                                                                                                                                                                                                          cAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       >  7:299889/1‑75 (MQ=255)
                                                                                                                                                                                                          cAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGAtgtg                                                                                                                                                                                                                                                                       <  8:299889/75‑1 (MQ=255)
                                                                                                                                                                                                            aaCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg                                                                                                                                                                                                                                                                >  5:777094/1‑80 (MQ=255)
                                                                                                                                                                                                            aaCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAg                                                                                                                                                                                                                                                                <  6:777094/80‑1 (MQ=255)
                                                                                                                                                                                                             aCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAgg                                                                                                                                                                                                              >  8:32590/1‑129 (MQ=255)
                                                                                                                                                                                                             aCTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAgg                                                                                                                                                                                                              <  7:32590/129‑1 (MQ=255)
                                                                                                                                                                                                                 cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGGGCGCAACAAGATGATGaa                                                         >  2:447148/1‑274 (MQ=255)
                                                                                                                                                                                                                 cACCGATGTTGGGGGCGTCAAAATCGCCGGGGGGTCCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGGGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                        <  4:250824/275‑1 (MQ=255)
                                                                                                                                                                                                                      aTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTTCGCCAGTGGCAATATGTTGCTTAGCCCATGGAAGGAGCGCAAAACAATAGTGAATCcaca                                                   >  4:471191/1‑275 (MQ=14)
                                                                                                                                                                                                                           ggtggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCCCCTTGCGCCAGTGGCAATATGTTCCCTAGCTCACGAAGGGAGCGCAAAAAGATGATCAATCAACATaaaa                                              >  4:291731/1‑275 (MQ=255)
                                                                                                                                                                                                                                cGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAccc                                                                                                                                                                                                                           >  6:22685/1‑97 (MQ=34)
                                                                                                                                                                                                                                cGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGAccc                                                                                                                                                                                                                           <  5:22685/97‑1 (MQ=34)
                                                                                                                                                                                                                                cGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaacaac                                          >  4:155338/1‑274 (MQ=255)
                                                                                                                                                                                                                                         tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtttt                                                                                                                                                                                                                                                      <  8:144657/62‑1 (MQ=33)
                                                                                                                                                                                                                                         tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGtttt                                                                                                                                                                                                                                                      >  7:144657/1‑62 (MQ=33)
                                                                                                                                                                                                                                         tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt                                                                                                                                                                                                                                            <  6:418949/72‑1 (MQ=32)
                                                                                                                                                                                                                                         tCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGt                                                                                                                                                                                                                                            >  5:418949/1‑72 (MQ=32)
                                                                                                                                                                                                                                                                       gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTc                                                                                                                                                                                                      <  8:1102144/80‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gCCCACATCCATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTc                                                                                                                                                                                                      >  7:1102144/1‑80 (MQ=255)
                                                                                                                                                                                                                                                                         ccACATGGAGGTGGGCTGTGGGCGCGTTGTACAGGGATGGTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg  <  2:236014/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                          
TAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCG  >  NC_000913/914811‑915347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: