Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,621 | A→G | 11.0% | intergenic (+87/‑109) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,621 | 0 | A | G | 11.0% | 196.1 / 12.6 | 73 | intergenic (+87/‑109) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (29/33); new base G (4/4); total (36/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01 |
GCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCA > NC_000913/164366‑164892 | gCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGTTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGCAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCCTCGCGCACGTTCACCCTCTCCCGCACCCTCCCCCTCAAGGgacacggctccgctc > 2:103318/1‑261 (MQ=255) gCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTTGGAGAGGGCCGGGGTGCGGG‑CACCAGCGCGGCGGGTCACCCTCACCCGACCCCTCTCCCCCCAGGGACGTGGGCCCGATcc > 4:380505/1‑274 (MQ=255) cACCTGCCCAAATGGCATTACAAAATACACGAGATTTGGGGGACTTCTGGAAAGGAGCGTACCGGGAGGGGCAAAAATTGCTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTCCACCGACGCGTCGGACGAAAAAGTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGGCCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 4:293377/274‑1 (MQ=255) atccaacGAGATTTGAGCGACTTCTGGGAAGGAGCGGACCGGGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 4:216241/270‑1 (MQ=255) acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt > 2:432662/1‑275 (MQ=255) gagaTTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGCGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg > 2:363040/1‑275 (MQ=255) aGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCCAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa > 2:123641/1‑275 (MQ=255) ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg > 2:201715/1‑275 (MQ=255) gtgcgggccAAAAAGAGATGATGGGGGNTNTTGCCAACCTTGTCTGGCGGGACGAGCCGGCAAGGACTGCACCGGNGCGACGGACGAAAAAGGATGTGGTAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGCGCCGGGGTGCGGN‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 3:47822/266‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTCTGGGAGAGGGCCGGGGGGAGGG‑CATCAGCGCGCACGTTCACCCGCACCCTAACCCTCTACCTCAAGGGAGAGGGGACCGAACGAGCACaa > 4:437520/1‑213 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt < 2:437521/213‑1 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCTCCCGTTAACCCTCACCCTAACCCTCCCCCCCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGATATATCTTCTTCTGTCTTGTAACAGAAGTACAGAAAATCGGGCTTTTGCGCCTTAat > 2:366426/1‑274 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa < 2:400389/273‑1 (MQ=255) gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCGGTCTTGTACCAGAAGACCAGAAAATCGGGCTTTTGCACCGGAATATTGCGGAGaa > 4:63793/1‑275 (MQ=255) tGAAAGGGCGTTATTCCAAACATGTCTGGCCGGACGACCAGGCAAATACTGCACGGATGCGAAGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa < 4:202366/274‑1 (MQ=255) gCGTTAGTCCAAACAGGTCGTGCCGGACGGCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCGCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTATCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAGCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATgg < 4:76522/275‑1 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGGAAAAGGATTCGTAAGGTTGGGTTTCTCCCGCTCCCTGGTGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCATCCTCACCCTACCCCCCCCCCTCCAGGGGGAGGGGACCGACCAGGCACAAATTCGGAGCGAAATCTGCTTCTGGCTTGGAACAGAAGAACAG‑AAATCGGGCTTTTGCGCCTGAATATTGCAGAGAAAGCGCATgtac > 4:319051/1‑272 (MQ=255) gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGTCGGACGAAAAAGCACTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGTGCGCACGTTCACCCCCACCCTAACCCCCTCCCTCAAGGGAGAGGGTACCGCTCGAGCATAAATTTTGAGCGATACCTTCTCCTGTCTTGTAACAGAAGAACTGTAATTCGGGCTTTTGCCCGTGAATATTGTGGAGAAAAATCATGGCCGGGATTGACcgcca > 4:64417/1‑273 (MQ=255) tCTGGCAGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGA‑CATCAGCGCGCACGTTCACCATCCTCCTAAACCTCTCCCTCAAGGGACAGGGGACCGATCGATCACAACATTTGAGAGATATCTTCTGCTGTCTGTTACCAGAAGAACAGAAAATCGGGCTTTTGCGCCGGTATATTTGCGAGTAAAAACATGACAGGGAATGGCCGagag > 4:61248/1‑275 (MQ=255) acCCGGAAAAGTCTGAAGCGGCGGGAACGAAGAAAAAGGAGTCGGAAGGTCGGTTTTCTCCCGTTACCGGTGGGAGCGGGCCGGGGGGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTACCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc < 4:253770/275‑1 (MQ=255) cccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 4:208901/219‑1 (MQ=255) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt > 7:981073/1‑148 (MQ=255) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 6:513753/121‑1 (MQ=255) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa > 5:513753/1‑121 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGGGCCAATTGGACGCAAAGGGAAACCGAcg > 2:514253/1‑275 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag > 5:889627/1‑126 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 6:889627/126‑1 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGCAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGAACAAAttt < 8:981073/148‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg > 1:127104/1‑146 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGCGCCAATTGGACGCAAAGGGAAAACGAagcagcc > 2:353325/1‑271 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCGTTCTTGTAACAGACGAACAGAAAATCGGGCTTTTTCTCCTGAATATTGCGGAGAAAAAGCTTGGCCGGGTATCACCCAGATCCCATTAGCATCACAGGGAAACCgcacggccc > 4:435264/1‑267 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag < 3:127104/146‑1 (MQ=255) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 8:333057/102‑1 (MQ=255) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa > 7:333057/1‑102 (MQ=255) aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 6:378585/101‑1 (MQ=255) aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag > 5:378585/1‑101 (MQ=255) ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTTTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAAGATTGTGGAGAAAACGCATTGCCGGTAATGACCCCGAGCCAATTTGAACCAAAGGGAAACCGACGCTTCCGGCCAACAAAAAGGTAAGcc > 4:396174/1‑275 (MQ=255) gTAAGGGTGGTTTTCCCCCTCCCCCGGTGGGAGAGGGTCCGGGTGAGGG‑CATCGGCGGGCACGTTACACCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgt < 4:201714/274‑1 (MQ=255) ttgtccccctcgcgctGTGGCAGATGGCCGGGGTGAGGG‑CATCAGCTCTCACGTTCACCCTCAGCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGNTCGATCACAAATTTTGTGTATTATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGGTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGa < 3:106275/229‑1 (MQ=255) tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 6:893299/81‑1 (MQ=255) tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa > 5:893299/1‑81 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa > 8:619513/1‑74 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 7:619513/74‑1 (MQ=255) tctcccgGGGGGGGAGGGCCGGGGGGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACg < 4:363039/274‑1 (MQ=255) ccctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc < 5:829662/147‑1 (MQ=255) ccctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc > 6:829662/1‑147 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg > 6:318569/1‑76 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 5:318569/76‑1 (MQ=255) gtgGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 5:354251/75‑1 (MQ=255) gtgGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg > 6:354251/1‑75 (MQ=255) tgGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTa > 2:418099/1‑273 (MQ=255) gagaGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTacgacg < 2:95429/275‑1 (MQ=255) ggCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGCATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGGCCGGACAAACAAAAGGTAAGACGTCGTCGGTAAGAAGATGATGATGATTACGACGTTTat > 2:324696/1‑275 (MQ=255) cGGGGTGAGGGCCACTAGC‑CGCACAGTCACCATCACCCTAACCCTCTCCCCCAAGGGAGAGGGGACCAATCGGGTACGAATTTTTAGTGATATCTTCTTCTTGCTTGTAACAGAAGAACAGAAAATCGGGCTTTTACGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTatgat < 4:485356/275‑1 (MQ=255) cGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTatgat < 4:418098/275‑1 (MQ=255) ggggggAGGG‑CAACAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGAACCACATAGAAAAAATTTTGAGAGATATCGTCTTCTGTCCTGTAACAGAAAAACAGAAAATCGGGCTTTTGCGCCGGAATATGGCGGAGAAAAAGCATGGCCGGGAGTGACCGCGAGCCGATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACAATGAATACGACGATTatgat < 4:103317/274‑1 (MQ=255) gg‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg < 7:760060/63‑1 (MQ=255) gg‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg > 8:760060/1‑63 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCTCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc < 8:649935/120‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTgag < 6:361149/76‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTgag > 5:361149/1‑76 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc > 7:649935/1‑120 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 7:323906/45‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag > 8:323906/1‑45 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAAc > 2:57589/1‑275 (MQ=255) tcaccctAACCCCTTCCCTCAAGGGAGAGGGGACCGATCGAGTACAAAATTTGGGAGATATCTTCTTCTGGTTTGGAACATAAGAACAGAAAATCGGGCTTTTGCGCCTGAAGATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCa < 2:287395/274‑1 (MQ=255) taacactACCCGTCTCCCTCAAGGGAGAGGGGACCGAGCGAGCACAAATTTTGAGAGATAGCTTCTTCTGTCTTGTAACAGAAGAACAGAAGATCGGGCTTTTGCGCCTTAAGATTGCAGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCa < 1:43313/269‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 7:246167/117‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg > 8:246167/1‑117 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAG‑AAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAggg < 2:319052/275‑1 (MQ=255) ctgtcCCTCAAGGGAGAGAGGACCGATCGAGCACAAATTTTGAGGGAGAGCTTCTTCTGTCTTGTAACAGAAAAACAGAAAGTCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGAAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggca < 2:64418/272‑1 (MQ=255) ctctCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 6:644601/61‑1 (MQ=255) ctctCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc > 5:644601/1‑61 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggca < 2:16998/275‑1 (MQ=255) tctcCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg > 7:1000038/1‑108 (MQ=255) tctcCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 8:1000038/108‑1 (MQ=255) ctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg > 6:141718/1‑135 (MQ=255) ctaccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg < 5:141718/132‑1 (MQ=255) cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg > 6:413278/1‑60 (MQ=255) cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 5:413278/60‑1 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggcaaaggca > 2:533956/1‑274 (MQ=255) aaGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 8:997329/1‑148 (MQ=255) | GCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGG‑CATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCA > NC_000913/164366‑164892 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |