Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,621 A→G 11.0% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,6210AG11.0% 196.1 / 12.6 73intergenic (+87/‑109)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (29/33);  new base G (4/4);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

GCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCA  >  NC_000913/164366‑164892
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
gCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGTTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGCAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCTCGCGCACGTTCACCCTCTCCCGCACCCTCCCCCTCAAGGgacacggctccgctc                                                                                                                                                                                                                                                               >  2:103318/1‑261 (MQ=255)
gCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTTGGAGAGGGCCGGGGTGCGGGCACCAGCGCGGCGGGTCACCCTCACCCGACCCCTCTCCCCCCAGGGACGTGGGCCCGATcc                                                                                                                                                                                                                                                              >  4:380505/1‑274 (MQ=255)
     cACCTGCCCAAATGGCATTACAAAATACACGAGATTTGGGGGACTTCTGGAAAGGAGCGTACCGGGAGGGGCAAAAATTGCTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTCCACCGACGCGTCGGACGAAAAAGTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGGCCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                                                                                                                                                                                          <  4:293377/274‑1 (MQ=255)
                            atccaacGAGATTTGAGCGACTTCTGGGAAGGAGCGGACCGGGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                                                                                                                                                                                                  <  4:216241/270‑1 (MQ=255)
                                 acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                                                                                                                                                                             >  2:432662/1‑275 (MQ=255)
                                   gagaTTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGCGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg                                                                                                                                                                                                                           >  2:363040/1‑275 (MQ=255)
                                           aGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCCAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                                                                                                                                                                                   >  2:123641/1‑275 (MQ=255)
                                                 ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                                                                                                                                                                                                             >  2:201715/1‑275 (MQ=255)
                                                                    gtgcgggccAAAAAGAGATGATGGGGGNTNTTGCCAACCTTGTCTGGCGGGACGAGCCGGCAAGGACTGCACCGGNGCGACGGACGAAAAAGGATGTGGTAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGCGCCGGGGTGCGGNCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                                                                                                                                                                           <  3:47822/266‑1 (MQ=255)
                                                                     tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTCTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCGCACCCTAACCCTCTACCTCAAGGGAGAGGGGACCGAACGAGCACaa                                                                                                                                                                                                                                                       >  4:437520/1‑213 (MQ=255)
                                                                         gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt                                                                                                                                                                                                                                                   <  2:437521/213‑1 (MQ=255)
                                                                          tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCTCCCGTTAACCCTCACCCTAACCCTCCCCCCCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGATATATCTTCTTCTGTCTTGTAACAGAAGTACAGAAAATCGGGCTTTTGCGCCTTAat                                                                                                                                                                                     >  2:366426/1‑274 (MQ=255)
                                                                          tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa                                                                                                                                                                                      <  2:400389/273‑1 (MQ=255)
                                                                                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCGGTCTTGTACCAGAAGACCAGAAAATCGGGCTTTTGCACCGGAATATTGCGGAGaa                                                                                                                                                                          >  4:63793/1‑275 (MQ=255)
                                                                                      tGAAAGGGCGTTATTCCAAACATGTCTGGCCGGACGACCAGGCAAATACTGCACGGATGCGAAGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa                                                                                                                                                                         <  4:202366/274‑1 (MQ=255)
                                                                                             gCGTTAGTCCAAACAGGTCGTGCCGGACGGCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCGCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTATCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAGCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATgg                                                                                                                                                                 <  4:76522/275‑1 (MQ=255)
                                                                                              cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGGAAAAGGATTCGTAAGGTTGGGTTTCTCCCGCTCCCTGGTGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCATCCTCACCCTACCCCCCCCCCTCCAGGGGGAGGGGACCGACCAGGCACAAATTCGGAGCGAAATCTGCTTCTGGCTTGGAACAGAAGAACAGAAATCGGGCTTTTGCGCCTGAATATTGCAGAGAAAGCGCATgtac                                                                                                                                                               >  4:319051/1‑272 (MQ=255)
                                                                                                             gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGTCGGACGAAAAAGCACTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGTGCGCACGTTCACCCCCACCCTAACCCCCTCCCTCAAGGGAGAGGGTACCGCTCGAGCATAAATTTTGAGCGATACCTTCTCCTGTCTTGTAACAGAAGAACTGTAATTCGGGCTTTTGCCCGTGAATATTGTGGAGAAAAATCATGGCCGGGATTGACcgcca                                                                                                                                                 >  4:64417/1‑273 (MQ=255)
                                                                                                              tCTGGCAGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGACATCAGCGCGCACGTTCACCATCCTCCTAAACCTCTCCCTCAAGGGACAGGGGACCGATCGATCACAACATTTGAGAGATATCTTCTGCTGTCTGTTACCAGAAGAACAGAAAATCGGGCTTTTGCGCCGGTATATTTGCGAGTAAAAACATGACAGGGAATGGCCGagag                                                                                                                                                >  4:61248/1‑275 (MQ=255)
                                                                                                                          acCCGGAAAAGTCTGAAGCGGCGGGAACGAAGAAAAAGGAGTCGGAAGGTCGGTTTTCTCCCGTTACCGGTGGGAGCGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTACCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc                                                                                                                                    <  4:253770/275‑1 (MQ=255)
                                                                                                                           cccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                                                                                                                                                                           <  4:208901/219‑1 (MQ=255)
                                                                                                                             cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                                                                                                                                                                                                >  7:981073/1‑148 (MQ=255)
                                                                                                                                       tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                                                                                                                                                 <  6:513753/121‑1 (MQ=255)
                                                                                                                                       tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                                                                                                                                                 >  5:513753/1‑121 (MQ=255)
                                                                                                                                         cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGGGCCAATTGGACGCAAAGGGAAACCGAcg                                                                                                                     >  2:514253/1‑275 (MQ=255)
                                                                                                                                          aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag                                                                                                                                                                                                                                                                         >  5:889627/1‑126 (MQ=255)
                                                                                                                                          aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag                                                                                                                                                                                                                                                                         <  6:889627/126‑1 (MQ=255)
                                                                                                                                          aCCGACGCGACGGACGAAAAAGTATTCGCAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGAACAAAttt                                                                                                                                                                                                                                                   <  8:981073/148‑1 (MQ=255)
                                                                                                                                              acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg                                                                                                                                                                                                                                                 >  1:127104/1‑146 (MQ=255)
                                                                                                                                              acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGCGCCAATTGGACGCAAAGGGAAAACGAagcagcc                                                                                                                >  2:353325/1‑271 (MQ=255)
                                                                                                                                              acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCGTTCTTGTAACAGACGAACAGAAAATCGGGCTTTTTCTCCTGAATATTGCGGAGAAAAAGCTTGGCCGGGTATCACCCAGATCCCATTAGCATCACAGGGAAACCgcacggccc                                                                                                                >  4:435264/1‑267 (MQ=255)
                                                                                                                                                  gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                                                                                                                                                                                             <  3:127104/146‑1 (MQ=255)
                                                                                                                                                          aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                                                                                                                                                 <  8:333057/102‑1 (MQ=255)
                                                                                                                                                          aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                                                                                                                                                 >  7:333057/1‑102 (MQ=255)
                                                                                                                                                                 aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                                                                                                                                                           <  6:378585/101‑1 (MQ=255)
                                                                                                                                                                 aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                                                                                                                                                           >  5:378585/1‑101 (MQ=255)
                                                                                                                                                                  ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTTTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAAGATTGTGGAGAAAACGCATTGCCGGTAATGACCCCGAGCCAATTTGAACCAAAGGGAAACCGACGCTTCCGGCCAACAAAAAGGTAAGcc                                                                                            >  4:396174/1‑275 (MQ=255)
                                                                                                                                                                     gTAAGGGTGGTTTTCCCCCTCCCCCGGTGGGAGAGGGTCCGGGTGAGGGCATCGGCGGGCACGTTACACCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgt                                                                                          <  4:201714/274‑1 (MQ=255)
                                                                                                                                                                               ttgtccccctcgcgctGTGGCAGATGGCCGGGGTGAGGGCATCAGCTCTCACGTTCACCCTCAGCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGNTCGATCACAAATTTTGTGTATTATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGGTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGa                                                                                                                       <  3:106275/229‑1 (MQ=255)
                                                                                                                                                                                tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                                                                                                                                                <  6:893299/81‑1 (MQ=255)
                                                                                                                                                                                tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                                                                                                                                                >  5:893299/1‑81 (MQ=255)
                                                                                                                                                                                       ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                                                                                                                                                >  8:619513/1‑74 (MQ=255)
                                                                                                                                                                                       ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                                                                                                                                                                                                <  7:619513/74‑1 (MQ=255)
                                                                                                                                                                                        tctcccgGGGGGGGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACg                                                                       <  4:363039/274‑1 (MQ=255)
                                                                                                                                                                                           ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                                                                                                                                                                                   <  5:829662/147‑1 (MQ=255)
                                                                                                                                                                                           ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                                                                                                                                                                                   >  6:829662/1‑147 (MQ=255)
                                                                                                                                                                                             ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                                                                                                                                                                                        >  6:318569/1‑76 (MQ=255)
                                                                                                                                                                                             ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                                                                                                                                                                                        <  5:318569/76‑1 (MQ=255)
                                                                                                                                                                                               gtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                                                                                                                                                                                       <  5:354251/75‑1 (MQ=255)
                                                                                                                                                                                               gtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                                                                                                                                                                                       >  6:354251/1‑75 (MQ=255)
                                                                                                                                                                                                tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTa                                                                >  2:418099/1‑273 (MQ=255)
                                                                                                                                                                                                   gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTacgacg                                                           <  2:95429/275‑1 (MQ=255)
                                                                                                                                                                                                        ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGCATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGGCCGGACAAACAAAAGGTAAGACGTCGTCGGTAAGAAGATGATGATGATTACGACGTTTat                                                      >  2:324696/1‑275 (MQ=255)
                                                                                                                                                                                                           cGGGGTGAGGGCCACTAGCCGCACAGTCACCATCACCCTAACCCTCTCCCCCAAGGGAGAGGGGACCAATCGGGTACGAATTTTTAGTGATATCTTCTTCTTGCTTGTAACAGAAGAACAGAAAATCGGGCTTTTACGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTatgat                                                   <  4:485356/275‑1 (MQ=255)
                                                                                                                                                                                                           cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTatgat                                                   <  4:418098/275‑1 (MQ=255)
                                                                                                                                                                                                            ggggggAGGGCAACAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGAACCACATAGAAAAAATTTTGAGAGATATCGTCTTCTGTCCTGTAACAGAAAAACAGAAAATCGGGCTTTTGCGCCGGAATATGGCGGAGAAAAAGCATGGCCGGGAGTGACCGCGAGCCGATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACAATGAATACGACGATTatgat                                                   <  4:103317/274‑1 (MQ=255)
                                                                                                                                                                                                                    ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg                                                                                                                                                                                                                                                              <  7:760060/63‑1 (MQ=255)
                                                                                                                                                                                                                    ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg                                                                                                                                                                                                                                                              >  8:760060/1‑63 (MQ=255)
                                                                                                                                                                                                                       cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCTCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                                                                                                                                                                                   <  8:649935/120‑1 (MQ=255)
                                                                                                                                                                                                                       cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTgag                                                                                                                                                                                                                                               <  6:361149/76‑1 (MQ=255)
                                                                                                                                                                                                                       cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTgag                                                                                                                                                                                                                                               >  5:361149/1‑76 (MQ=255)
                                                                                                                                                                                                                       cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc                                                                                                                                                                                                   >  7:649935/1‑120 (MQ=255)
                                                                                                                                                                                                                          cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                                                                                                                                                           <  7:323906/45‑1 (MQ=255)
                                                                                                                                                                                                                          cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                                                                                                                                                           >  8:323906/1‑45 (MQ=255)
                                                                                                                                                                                                                               cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAAc                                >  2:57589/1‑275 (MQ=255)
                                                                                                                                                                                                                                            tcaccctAACCCCTTCCCTCAAGGGAGAGGGGACCGATCGAGTACAAAATTTGGGAGATATCTTCTTCTGGTTTGGAACATAAGAACAGAAAATCGGGCTTTTGCGCCTGAAGATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCa                    <  2:287395/274‑1 (MQ=255)
                                                                                                                                                                                                                                            taacactACCCGTCTCCCTCAAGGGAGAGGGGACCGAGCGAGCACAAATTTTGAGAGATAGCTTCTTCTGTCTTGTAACAGAAGAACAGAAGATCGGGCTTTTGCGCCTTAAGATTGCAGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCa                    <  1:43313/269‑1 (MQ=255)
                                                                                                                                                                                                                                               ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                                                                                                                                                              <  7:246167/117‑1 (MQ=255)
                                                                                                                                                                                                                                               ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                                                                                                                                                              >  8:246167/1‑117 (MQ=255)
                                                                                                                                                                                                                                                    aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAggg          <  2:319052/275‑1 (MQ=255)
                                                                                                                                                                                                                                                       ctgtcCCTCAAGGGAGAGAGGACCGATCGAGCACAAATTTTGAGGGAGAGCTTCTTCTGTCTTGTAACAGAAAAACAGAAAGTCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGAAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggca        <  2:64418/272‑1 (MQ=255)
                                                                                                                                                                                                                                                       ctctCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc                                                                                                                                                                                                                              <  6:644601/61‑1 (MQ=255)
                                                                                                                                                                                                                                                       ctctCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc                                                                                                                                                                                                                              >  5:644601/1‑61 (MQ=255)
                                                                                                                                                                                                                                                       ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggca        <  2:16998/275‑1 (MQ=255)
                                                                                                                                                                                                                                                        tctcCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                                                                                                                                                              >  7:1000038/1‑108 (MQ=255)
                                                                                                                                                                                                                                                        tctcCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                                                                                                                                                              <  8:1000038/108‑1 (MQ=255)
                                                                                                                                                                                                                                                         ctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg                                                                                                                                                  >  6:141718/1‑135 (MQ=255)
                                                                                                                                                                                                                                                         ctaccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg                                                                                                                                                  <  5:141718/132‑1 (MQ=255)
                                                                                                                                                                                                                                                           cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg                                                                                                                                                                                                                           >  6:413278/1‑60 (MQ=255)
                                                                                                                                                                                                                                                           cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg                                                                                                                                                                                                                           <  5:413278/60‑1 (MQ=255)
                                                                                                                                                                                                                                                              tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGggcaaaggca  >  2:533956/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                aaGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg                                                                                                                              >  8:997329/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                               
GCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGACGATTATGATGACTATGAGGATGAAGAACCGATGCCGCGCAAAGGTAAGGGCAAAGGCA  >  NC_000913/164366‑164892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: