Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,227,051 G→C 8.2% intergenic (+83/+20) ycgJ → / ← pliG uncharacterized protein/periplasmic inhibitor of g‑type lysozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,227,0510GC 8.2% 194.8 / 8.8 61intergenic (+83/+20)ycgJ/pliGuncharacterized protein/periplasmic inhibitor of g‑type lysozyme
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base C (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

GGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAG  >  NC_000913/1226777‑1227311
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                    
ggCGAAAAGTCCGCTGAAAACTTACAACCAATTGAAGGCTACGATCCCAGCGAATGTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGGAAGCGGAGTGGTAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      <  2:489264/275‑1 (MQ=255)
 gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgaga                                                                                                                                                                                                                                                                     >  4:242731/1‑275 (MQ=255)
               gAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGtt                                                                                                                                                                                                                                                        <  2:527496/274‑1 (MQ=255)
                                                                         tATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATAGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAACATCAATCCAACCACCACAAAGATGTTATTTATGTTTCGTGAGTGATGTCTTGTTNACATTATGATCTATTCGTCATTAACCTCTTGCGCGACAGAGAGGAGTGGGGCATTACCGTTTATGACGGNGAGAGTCCTCGTCGGTTGACATTTACTTAATTTGAATAGAGGAGTNTNATTTTTTAGTTTGGttttttgggggt                                                                                                                                                                                               >  3:43144/1‑266 (MQ=255)
                                                                               gCGATGTTAAAGAAAAATTATGCCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGCCGGTTGACATTTCCTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTAGTTTc                                                                                                                                                                                        >  2:446840/1‑274 (MQ=255)
                                                                                                 tATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAg                                                                                                                                                                     <  2:242732/275‑1 (MQ=255)
                                                                                                         gatgatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCTTCGTTTCGGGTTTGCAACACTCGCCGCTCATAtt                                                                                                                                                             >  1:142802/1‑275 (MQ=255)
                                                                                                            tatCGTTATTTTGGTGGGGATGGTAAAAGGGGTAGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTGTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                                                                                                                                          <  4:444741/274‑1 (MQ=255)
                                                                                                            gatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                                                                                                                                          <  1:74593/275‑1 (MQ=255)
                                                                                                                          tgtgGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGTCATTAACGTTTATGACGGGGAGAGTCGCCGTCGGTTGACATTTTCTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCTGGTTTGCAACACTCTCAGCTCATTTTTGCCACAAGCAGGgagt                                                                                                                                            >  4:278569/1‑275 (MQ=255)
                                                                                                                               aTGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      >  7:1131945/1‑148 (MQ=255)
                                                                                                                                            tGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                         <  8:1131945/148‑1 (MQ=255)
                                                                                                                                               aaaaaTCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      <  6:953766/132‑1 (MQ=255)
                                                                                                                                               aaaaaTCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      >  5:953766/1‑132 (MQ=255)
                                                                                                                                                      aatcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGATAGGAGTGGGGCATTAACGTTTATGACTGGGAGAGTCCCCGTCGGTTGACAtt                                                                                                                                                                                                                                                  >  1:26489/1‑145 (MQ=255)
                                                                                                                                                        tcaaACCACCACAAAGATGTTATTTATGTGTCGTGCGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGCCAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCTGGGAGTGAGTATTTTCCGTGACTATCGACTTCTggg                                                                                                              >  1:66630/1‑275 (MQ=255)
                                                                                                                                                        tcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACtt                                                                                                                                                                                                                                             >  5:467659/1‑148 (MQ=255)
                                                                                                                                                         caaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTACGACGGGGACTCTCCCCGTcataa                                                                                                                                                                                                                                                        >  7:867035/1‑132 (MQ=255)
                                                                                                                                                         caaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTACGACGGGGACTCTCCCCGTcataa                                                                                                                                                                                                                                                        <  8:867035/136‑5 (MQ=255)
                                                                                                                                                          aagccancaCAAANATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACt                                                                                                                                                                                                                                              <  3:26489/142‑1 (MQ=255)
                                                                                                                                                            accaccACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      >  5:321703/1‑119 (MQ=255)
                                                                                                                                                            accaccACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      <  6:321703/119‑1 (MQ=255)
                                                                                                                                                               accacAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGa                                                                                                                                                                                                                                      >  5:1014235/1‑148 (MQ=255)
                                                                                                                                                                      aGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      <  8:622669/109‑1 (MQ=255)
                                                                                                                                                                      aGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                                                                                                                                      >  7:622669/1‑109 (MQ=255)
                                                                                                                                                                               ttatGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCg                                                                                                                                                                                                                                                              >  6:404082/1‑108 (MQ=255)
                                                                                                                                                                               ttatGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCg                                                                                                                                                                                                                                                              <  5:404082/108‑1 (MQ=255)
                                                                                                                                                                                  tgtgTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAat                                                                                     >  4:234790/1‑274 (MQ=255)
                                                                                                                                                                                   gtgtCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCg                                                                                                                                                                                                                                >  7:557193/1‑134 (MQ=255)
                                                                                                                                                                                   gtgtCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCg                                                                                                                                                                                                                                <  8:557193/134‑1 (MQ=255)
                                                                                                                                                                                       cGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                                                                                                                                                <  6:876759/114‑1 (MQ=255)
                                                                                                                                                                                       cGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                                                                                                                                                >  5:876759/1‑114 (MQ=255)
                                                                                                                                                                                              gaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                           >  5:1024219/1‑112 (MQ=255)
                                                                                                                                                                                              gaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                           <  6:1024219/112‑1 (MQ=255)
                                                                                                                                                                                                tGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                   >  3:30796/1‑102 (MQ=255)
                                                                                                                                                                                                   cTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttt                                                                                                                                                                                                                    <  7:762172/130‑1 (MQ=255)
                                                                                                                                                                                                   cTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttt                                                                                                                                                                                                                    >  8:762172/1‑130 (MQ=255)
                                                                                                                                                                                                    ttGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTAc                                                                                                                                                                                                                                               <  1:30796/102‑1 (MQ=255)
                                                                                                                                                                                                     tGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTATCGTTTATGACGGGGAGAGTGCCCGTCgg                                                                                                                                                                                                                                                          >  8:453619/1‑90 (MQ=255)
                                                                                                                                                                                                     tGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCgg                                                                                                                                                                                                                                                          <  7:453619/90‑1 (MQ=255)
                                                                                                                                                                                                                 gTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                   <  8:1102854/85‑1 (MQ=255)
                                                                                                                                                                                                                 gTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                   >  7:1102854/1‑85 (MQ=255)
                                                                                                                                                                                                                 gTTCTATTCGTCATGAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTAATTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCt                                                                                                                                                                   >  2:551237/1‑165 (MQ=255)
                                                                                                                                                                                                                   tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                         >  8:71175/1‑77 (MQ=255)
                                                                                                                                                                                                                   tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                         <  7:71175/77‑1 (MQ=255)
                                                                                                                                                                                                                   tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGt                                                                                                                                                                                                                            >  8:649378/1‑106 (MQ=255)
                                                                                                                                                                                                                   tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGt                                                                                                                                                                                                                            <  7:649378/106‑1 (MQ=255)
                                                                                                                                                                                                                     tATTCGTCCTTAACCTCTTGAGCGCCACAGCGGAGTGGGGCATTAACGTTTATGACGGCGAGAGTCCACGTCGGTTGACATTTACTTAATTTGAATATCGCCGTTATATTTTTTACCTTTGTTTTTACGGTCGTCGTTTCGGGTTTGCAACACTCGCCGCTCata                                                                                                                                                               <  4:551236/165‑1 (MQ=255)
                                                                                                                                                                                                                     tATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                           <  5:470774/89‑1 (MQ=255)
                                                                                                                                                                                                                     tATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                           >  6:470774/1‑89 (MQ=255)
                                                                                                                                                                                                                        tCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGt                                                                                                                                                                                                                                                             >  7:90903/1‑68 (MQ=255)
                                                                                                                                                                                                                        tCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGt                                                                                                                                                                                                                                                             <  8:90903/68‑1 (MQ=255)
                                                                                                                                                                                                                                 aCCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                                                                                                                                                                                                                 >  8:789655/1‑71 (MQ=255)
                                                                                                                                                                                                                                 aCCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                                                                                                                                                                                                                 <  7:789655/71‑1 (MQ=255)
                                                                                                                                                                                                                                           cGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATg                           <  2:184758/275‑1 (MQ=255)
                                                                                                                                                                                                                                              cAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                         >  6:184428/1‑50 (MQ=255)
                                                                                                                                                                                                                                              cAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                         <  5:184428/50‑1 (MQ=255)
                                                                                                                                                                                                                                                gagagGCGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTCGACATCTACTTAATTTGAATATCGACGCTATATTTTTTAGTTTTGTTTTTACGGGCGTCGCTTCGGGTTTGCAACATTCGCAGCTCATTTTTGCAAGAAGCAGGGAGTGAGTATTGTCCGTGCCTATCGAGTTCTGGGGATTATCTGGATAAATCATCTGAATCATCGATTCCTGGCCACAAGAGGTAATTACAAACCCCTTCCTTACAGAAACCCACACGCACtc                      >  4:130140/1‑274 (MQ=255)
                                                                                                                                                                                                                                                  gaggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                                                                                                                                                                                                               >  6:124910/1‑72 (MQ=255)
                                                                                                                                                                                                                                                  gaggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                                                                                                                                                                                                               <  5:124910/72‑1 (MQ=255)
                                                                                                                                                                                                                                                    ggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                                                                                                                                                 <  6:1014235/148‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTgc                                                                        >  4:113428/1‑216 (MQ=255)
                                                                                                                                                                                                                                                                tAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGc      >  2:77072/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                     tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg  <  4:77071/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tgtaggACGGTGAGAGTCCCCTTCGATTGACATTTACTTAATTTGAATATCGGCGTTATATTTTTTAGTTTGGTTTTTACGGGCGTCGTTTCGGGTTTTCAACCCTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg  <  1:43144/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tttttaCGGTGGGAGTCATCGTTGGTTNNTATTTATTTACTTTGATTTTCGTCTTTGTTNTTTTTAGTTTTTTTTTTGCGAGCGACGTTTAGGGTTTGCCACACTCGCAGCTCATTTTTGCCAGAAGCAGGGAGNGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTCGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg  <  3:66630/268‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aCGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttg                                                                                                                                                                                                           <  6:326403/67‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgt                                                                                                                                                                                                               <  6:313083/62‑1 (MQ=255)
                                                                                                                                                                                                                                                                            cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgt                                                                                                                                                                                                               >  5:313083/1‑62 (MQ=255)
                                                                                                                                                                                                                                                                              gggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCg                                                                                                                       <  6:467659/148‑1 (MQ=255)
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GGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAG  >  NC_000913/1226777‑1227311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: