Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,227,052 A→T 8.2% intergenic (+84/+19) ycgJ → / ← pliG uncharacterized protein/periplasmic inhibitor of g‑type lysozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,227,0520AT 8.2% 188.7 / 8.3 61intergenic (+84/+19)ycgJ/pliGuncharacterized protein/periplasmic inhibitor of g‑type lysozyme
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base T (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

GCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCG  >  NC_000913/1226778‑1227326
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                  
gCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgaga                                                                                                                                                                                                                                                                                    >  4:242731/1‑275 (MQ=255)
              gAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGtt                                                                                                                                                                                                                                                                       <  2:527496/274‑1 (MQ=255)
                                                                        tATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATAGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAACATCAATCCAACCACCACAAAGATGTTATTTATGTTTCGTGAGTGATGTCTTGTTNACATTATGATCTATTCGTCATTAACCTCTTGCGCGACAGAGAGGAGTGGGGCATTACCGTTTATGACGGNGAGAGTCCTCGTCGGTTGACATTTACTTAATTTGAATAGAGGAGTNTNATTTTTTAGTTTGGttttttgggggt                                                                                                                                                                                                              >  3:43144/1‑266 (MQ=255)
                                                                              gCGATGTTAAAGAAAAATTATGCCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGCCGGTTGACATTTCCTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTAGTTTc                                                                                                                                                                                                       >  2:446840/1‑274 (MQ=255)
                                                                                                tATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAg                                                                                                                                                                                    <  2:242732/275‑1 (MQ=255)
                                                                                                        gatgatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCTTCGTTTCGGGTTTGCAACACTCGCCGCTCATAtt                                                                                                                                                                            >  1:142802/1‑275 (MQ=255)
                                                                                                           tatCGTTATTTTGGTGGGGATGGTAAAAGGGGTAGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTGTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                                                                                                                                                         <  4:444741/274‑1 (MQ=255)
                                                                                                           gatCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                                                                                                                                                         <  1:74593/275‑1 (MQ=255)
                                                                                                                         tgtgGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGTCATTAACGTTTATGACGGGGAGAGTCGCCGTCGGTTGACATTTTCTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCTGGTTTGCAACACTCTCAGCTCATTTTTGCCACAAGCAGGgagt                                                                                                                                                           >  4:278569/1‑275 (MQ=255)
                                                                                                                                           tGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                                        <  8:1131945/148‑1 (MQ=255)
                                                                                                                                                     aatcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGATAGGAGTGGGGCATTAACGTTTATGACTGGGAGAGTCCCCGTCGGTTGACAtt                                                                                                                                                                                                                                                                 >  1:26489/1‑145 (MQ=255)
                                                                                                                                                       tcaaACCACCACAAAGATGTTATTTATGTGTCGTGCGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGCCAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCTGGGAGTGAGTATTTTCCGTGACTATCGACTTCTggg                                                                                                                             >  1:66630/1‑275 (MQ=255)
                                                                                                                                                       tcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACtt                                                                                                                                                                                                                                                            >  5:467659/1‑148 (MQ=255)
                                                                                                                                                        caaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTACGACGGGGACTCTCCCCGTcataa                                                                                                                                                                                                                                                                       <  8:867035/136‑5 (MQ=255)
                                                                                                                                                        caaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTACGACGGGGACTCTCCCCGTcataa                                                                                                                                                                                                                                                                       >  7:867035/1‑132 (MQ=255)
                                                                                                                                                         aagccancaCAAANATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACt                                                                                                                                                                                                                                                             <  3:26489/142‑1 (MQ=255)
                                                                                                                                                              accacAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGa                                                                                                                                                                                                                                                     >  5:1014235/1‑148 (MQ=255)
                                                                                                                                                                              ttatGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCg                                                                                                                                                                                                                                                                             >  6:404082/1‑108 (MQ=255)
                                                                                                                                                                              ttatGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCg                                                                                                                                                                                                                                                                             <  5:404082/108‑1 (MQ=255)
                                                                                                                                                                                 tgtgTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAat                                                                                                    >  4:234790/1‑274 (MQ=255)
                                                                                                                                                                                  gtgtCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCg                                                                                                                                                                                                                                               >  7:557193/1‑134 (MQ=255)
                                                                                                                                                                                  gtgtCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCg                                                                                                                                                                                                                                               <  8:557193/134‑1 (MQ=255)
                                                                                                                                                                                      cGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                                                                                                                                                               <  6:876759/114‑1 (MQ=255)
                                                                                                                                                                                      cGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                                                                                                                                                               >  5:876759/1‑114 (MQ=255)
                                                                                                                                                                                             gaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                                          <  6:1024219/112‑1 (MQ=255)
                                                                                                                                                                                             gaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                                          >  5:1024219/1‑112 (MQ=255)
                                                                                                                                                                                               tGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                                  >  3:30796/1‑102 (MQ=255)
                                                                                                                                                                                                  cTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttt                                                                                                                                                                                                                                   >  8:762172/1‑130 (MQ=255)
                                                                                                                                                                                                  cTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttt                                                                                                                                                                                                                                   <  7:762172/130‑1 (MQ=255)
                                                                                                                                                                                                   ttGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTAc                                                                                                                                                                                                                                                              <  1:30796/102‑1 (MQ=255)
                                                                                                                                                                                                    tGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTATCGTTTATGACGGGGAGAGTGCCCGTCgg                                                                                                                                                                                                                                                                         >  8:453619/1‑90 (MQ=255)
                                                                                                                                                                                                    tGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCgg                                                                                                                                                                                                                                                                         <  7:453619/90‑1 (MQ=255)
                                                                                                                                                                                                                gTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                                  <  8:1102854/85‑1 (MQ=255)
                                                                                                                                                                                                                gTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAt                                                                                                                                                                                                                                                                  >  7:1102854/1‑85 (MQ=255)
                                                                                                                                                                                                                gTTCTATTCGTCATGAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTAATTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCt                                                                                                                                                                                  >  2:551237/1‑165 (MQ=255)
                                                                                                                                                                                                                  tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                                        <  7:71175/77‑1 (MQ=255)
                                                                                                                                                                                                                  tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                                        >  8:71175/1‑77 (MQ=255)
                                                                                                                                                                                                                  tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGt                                                                                                                                                                                                                                           >  8:649378/1‑106 (MQ=255)
                                                                                                                                                                                                                  tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGt                                                                                                                                                                                                                                           <  7:649378/106‑1 (MQ=255)
                                                                                                                                                                                                                    tATTCGTCCTTAACCTCTTGAGCGCCACAGCGGAGTGGGGCATTAACGTTTATGACGGCGAGAGTCCACGTCGGTTGACATTTACTTAATTTGAATATCGCCGTTATATTTTTTACCTTTGTTTTTACGGTCGTCGTTTCGGGTTTGCAACACTCGCCGCTCata                                                                                                                                                                              <  4:551236/165‑1 (MQ=255)
                                                                                                                                                                                                                    tATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                                          <  5:470774/89‑1 (MQ=255)
                                                                                                                                                                                                                    tATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTaa                                                                                                                                                                                                                                                          >  6:470774/1‑89 (MQ=255)
                                                                                                                                                                                                                       tCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGt                                                                                                                                                                                                                                                                            >  7:90903/1‑68 (MQ=255)
                                                                                                                                                                                                                       tCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGt                                                                                                                                                                                                                                                                            <  8:90903/68‑1 (MQ=255)
                                                                                                                                                                                                                                aCCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                                                                                                                                                                                                                                >  8:789655/1‑71 (MQ=255)
                                                                                                                                                                                                                                aCCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACAttt                                                                                                                                                                                                                                                                <  7:789655/71‑1 (MQ=255)
                                                                                                                                                                                                                                          cGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATg                                          <  2:184758/275‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                                        >  6:184428/1‑50 (MQ=255)
                                                                                                                                                                                                                                             cAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGt                                                                                                                                                                                                                                                                        <  5:184428/50‑1 (MQ=255)
                                                                                                                                                                                                                                               gagagGCGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTCGACATCTACTTAATTTGAATATCGACGCTATATTTTTTAGTTTTGTTTTTACGGGCGTCGCTTCGGGTTTGCAACATTCGCAGCTCATTTTTGCAAGAAGCAGGGAGTGAGTATTGTCCGTGCCTATCGAGTTCTGGGGATTATCTGGATAAATCATCTGAATCATCGATTCCTGGCCACAAGAGGTAATTACAAACCCCTTCCTTACAGAAACCCACACGCACtc                                     >  4:130140/1‑274 (MQ=255)
                                                                                                                                                                                                                                                 gaggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                                                                                                                                                                                                                              >  6:124910/1‑72 (MQ=255)
                                                                                                                                                                                                                                                 gaggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGa                                                                                                                                                                                                                                              <  5:124910/72‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                                                                                                                                                                <  6:1014235/148‑1 (MQ=255)
                                                                                                                                                                                                                                                       tGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTgc                                                                                       >  4:113428/1‑216 (MQ=255)
                                                                                                                                                                                                                                                               tAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGc                     >  2:77072/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                    tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg                 <  4:77071/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tgtaggACGGTGAGAGTCCCCTTCGATTGACATTTACTTAATTTGAATATCGGCGTTATATTTTTTAGTTTGGTTTTTACGGGCGTCGTTTCGGGTTTTCAACCCTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg                 <  1:43144/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tttttaCGGTGGGAGTCATCGTTGGTTNNTATTTATTTACTTTGATTTTCGTCTTTGTTNTTTTTAGTTTTTTTTTTGCGAGCGACGTTTAGGGTTTGCCACACTCGCAGCTCATTTTTGCCAGAAGCAGGGAGNGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTCGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAg                 <  3:66630/268‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aCGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttg                                                                                                                                                                                                                          <  6:326403/67‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgt                                                                                                                                                                                                                              >  5:313083/1‑62 (MQ=255)
                                                                                                                                                                                                                                                                           cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgt                                                                                                                                                                                                                              <  6:313083/62‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCg                                                                                                                                      <  6:467659/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  agTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTatcg  <  2:234791/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                  
GCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCG  >  NC_000913/1226778‑1227326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: