Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,541,077 | G→C | 6.7% | A505G (GCC→GGC) | narZ ← | nitrate reductase 2 (NRZ), alpha subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,541,077 | 0 | G | C | 6.7% | 511.2 / 22.8 | 150 | A505G (GCC→GGC) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
Reads supporting (aligned to +/- strand): ref base G (73/63); new base C (4/6); total (80/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.17e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-03 |
Only 100 of 154 total aligned reads displayed.
GCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA > NC_000913/1540805‑1541348 | gCCATTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGAACGAGTGTGACCAGCCGCCACCGCTGTGCCCGACCCAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTa < 4:90901/275‑1 (MQ=255) ccGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTATGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATCTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATACTCATCCAGCGCCAATGCGTTTTATGGGCGGTATAGGCCAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTACTTTGCTCACCCCCGTCTGGGGTTTTCGGTTGAAtttgtgg > 4:466085/1‑271 (MQ=255) ccGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCAGCCCCCGCATTACCCGACCCAACCGCAGAAGATCGGCATATTGATCATACCGCGGTAGTTCATGTCCATTGGATACACGTGGTTAACACCTGCGCAGTGGATAATCATCGAGCGCTCATGCGTTTTCTGGGCGGTATCGGCAAATTCAAGGGCAAGGGTTTCGATTTAACTGCCG‑GGGACGCCAGGTTA‑TTGCCCAACCCACGCCGGGGTGTGCGGGTTGACTTctg > 4:508/1‑272 (MQ=255) ccGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATTCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTc > 2:405268/1‑275 (MQ=255) aTAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTNTTATGGGCGGTAACGGCAAATTCACGGGCGATGGTTTCACTAT‑ACTGGCGCGGAACACC‑GGTAATTTGCTCCCCNCCGGATGGGGGGTTCGGTTTGATTTTAGCGNNATCTTTTAC‑CTGTTTTTCATTTTCCac > 3:75338/1‑272 (MQ=255) cccAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATc > 2:78572/1‑274 (MQ=255) gccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGAGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGAACGCC‑GGTAATTTGCTCAACCCAGTCTGGGGTGTACGGTTTGATTTCAGCGTCATCTTTCTCACTG‑TTTTCATCTTCACGCCcccgttgcaggc > 4:384209/1‑267 (MQ=255) gccacgacggtgttGCCCGACAAACCCGGAGAGGCTCAGCAAGTGGATCAACCTGGGGTTGGTCATGTGATTGTGATACCAGTGGTTACCCGCTGGCGCGAGGATAAGCACCCGGGCACAAGGCGTTTTATAGGGGGTATCGGCAAATTCACGGGCGATGGTTTCAAGAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGCGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGc < 4:283967/263‑1 (MQ=255) ccGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTACTTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCAGTACTTCGCCAGTACCAAATCATa > 1:115687/1‑275 (MQ=255) ccGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCACGTGATACCAGTGGTTAACACCTGCCCCGAGGATAATCATCGAGCGCCCATGCGTTTTAAGGGCGGTATGCGCAAATTCCCGGGCGATGGTTTCAATCT‑ACTGGCCCGGCACGCC‑GGTAATTTGCTCACCCCAGTCTGGGGTGGACGGTTTGAGTTCAGCGTAATATTTCGTCCGG‑TTTTCATCTTCCAGCCCGCCATCGAGGCCGTAATTCACACGTCCCAAATCATa > 4:452965/1‑275 (MQ=255) gaagaTCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGGACCAAATCATAAACGc > 1:114923/1‑275 (MQ=255) tatTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc > 4:251949/1‑187 (MQ=255) gATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCg < 2:251950/187‑1 (MQ=255) atcatcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGgt > 2:319269/1‑275 (MQ=255) gcgGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCa < 2:374532/274‑1 (MQ=255) ttCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTg < 8:249620/148‑1 (MQ=255) ttCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGAACACAGGACAGGTGTTGCCATCGACTa > 2:79879/1‑274 (MQ=255) tGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGCGTTGCCATCGACTAACGTc < 2:231781/275‑1 (MQ=255) tGTCCATGGGATTCCGGTGTTTAACACCTGCGCCGAAGATGATCATCGAGGGCCCATGCGTTTTATGGGCGGGATCGTCAAATTGACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGTCTGGGGTGTACGGTTTTATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTc < 4:79878/275‑1 (MQ=255) gTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTc > 2:371728/1‑274 (MQ=255) gTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTc > 4:319852/1‑274 (MQ=255) ccATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCCGTACCAAATCATACACGCTGACCACCGGACAGGTGTTGCCATCGCCTACCGTCAgg > 1:69033/1‑275 (MQ=255) aTGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGgtgt > 6:1075670/1‑145 (MQ=255) aTGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGCCTGGGgtg < 8:1090200/144‑1 (MQ=255) gtACCAGTGGTTAAAACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGt < 2:388838/263‑1 (MQ=255) aGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc < 7:918649/148‑1 (MQ=255) tGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc < 5:379923/146‑1 (MQ=255) ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCACGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAgg > 6:999267/1‑121 (MQ=255) tAACACCTGCGCCTAGGATAATCAGCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGTCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTgg < 1:34506/275‑1 (MQ=255) cacCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAACTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATc > 5:795807/1‑148 (MQ=255) aacTGCGCCGAGGATAAGAATCGTGCGCACATACGTTTTATGGGCGGTATCGGCAAATTCACGTGCGAGGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTAACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGgcgc < 2:538287/272‑1 (MQ=255) cTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTACTTTGCTCACCCCAGGCCGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCCTCTTCCAGCTCGCGATCGAGCCCGTAATTCGCCAGTACCAAATCATAAACCCTTACCACCGGACAGGTTTCGCCATCGCCGAACGTCAGGTTTTTAACGGGCCATTGGCCCAcc > 4:300767/1‑275 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGaccac > 4:144935/1‑218 (MQ=255) ggATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑tt > 5:447586/1‑148 (MQ=255) ggATAATCATCGAGAGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGa < 2:480417/275‑1 (MQ=255) aTAATCCTCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCATGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGTTTTTTAACGGGCAATTGGCGCACCAGCACCGGAtt < 2:529983/275‑1 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTgg > 7:1070921/1‑107 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑tttt < 6:447586/148‑1 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑t < 8:278444/145‑1 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑t > 7:278444/1‑145 (MQ=255) atcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTgg > 8:215975/1‑104 (MQ=255) tcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGCCTGGGgtg < 7:88405/107‑1 (MQ=255) tcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGCCTGGGgtg > 8:88405/1‑107 (MQ=255) tcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGt > 6:157295/1‑110 (MQ=255) tcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGt < 5:157295/110‑1 (MQ=255) tcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTc > 7:738684/1‑148 (MQ=255) tcGAGCGCCCATGCGTTTCATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTc > 7:671894/1‑148 (MQ=255) tttATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc < 7:314569/102‑1 (MQ=255) tATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGCCAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCCCCAGCCCCGGGTTGTGTTTTACGCTGCGAACATGCgg > 2:18339/1‑274 (MQ=255) tATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCgg > 2:264025/1‑274 (MQ=255) gTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGAATAACGTCAGGTTTTTAACGTGCAATTGGTGCACCCGCCCCGGCTTTGATTTTACGCGGCGAAAATGCGGAATTTAAAt > 1:44423/1‑275 (MQ=255) aTCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGcc > 5:49115/1‑148 (MQ=255) aTCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGcc > 5:630886/1‑148 (MQ=255) tCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCa < 8:671894/148‑1 (MQ=255) gCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTa > 5:482798/1‑137 (MQ=255) tCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTc < 8:1008881/110‑1 (MQ=255) ggCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTgg < 7:222346/57‑1 (MQ=255) ggCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaa > 8:677472/1‑148 (MQ=255) ggCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGa < 2:136484/275‑1 (MQ=255) gCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTgg > 8:626430/1‑56 (MQ=255) gCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTgg < 7:626430/56‑1 (MQ=255) cGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGttt < 8:1012943/67‑1 (MQ=255) cGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGttt > 7:1012943/1‑67 (MQ=255) aTGGTTTCATTAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑t > 7:68765/1‑91 (MQ=255) aTGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑t < 8:68765/91‑1 (MQ=255) tGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGcc < 2:319853/275‑1 (MQ=255) ggTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTg < 7:677472/148‑1 (MQ=255) tttCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGgtgt > 6:30699/1‑54 (MQ=255) tttCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGgtgt < 5:30699/54‑1 (MQ=255) ttCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGAt > 6:260506/1‑107 (MQ=255) tCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGacca < 6:384275/148‑1 (MQ=255) att‑aCCGGCGTGCCGCGCC‑AGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTg > 6:902604/3‑56 (MQ=21) att‑aCCGGCGTGCCGCGCC‑AGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTg < 5:902604/54‑1 (MQ=21) gaCTGGCGCGGTCCGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCTCGATCGAGGCCGTAATTCGCCAGTACCAAATCAGAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCaa < 4:264024/273‑1 (MQ=255) aCTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACa < 6:49115/148‑1 (MQ=255) tGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACCATGCGAAAATGCGGAATTTCAATGCCGCCAAACTAGGGTAAGGCCACGCATGCAACAGc > 4:139818/1‑275 (MQ=255) ggCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTa > 7:174301/1‑106 (MQ=255) cgcgGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGCTGGTGTTGTACGCTGCGAAAATGCGGATTTTCAATGCCGCAAAAGTAGGGGAAGGCCACGCCTGAAAGAGcagc > 4:133247/1‑272 (MQ=255) cgcgGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGCCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTTTGTTTTACGTTGCGAAACTCAGGATTTTAAATGCCGCCAATGTAGGGGAAGGCCACGCCTCCAACAGCGTc > 4:123600/1‑275 (MQ=255) cgGCACGCC‑GGTAATTTGCCCACACCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGACAGTACCAAATCAGAAACGCTGACCACCGGCCAGGTGTTGCCATCGACTAACGTCAGGTTCTAAACGGGCAATCGGCGCCACAGCACCGGCTAGTGTTTTACGCTGCCAAAATGCGGCCTGTCAACGCCGCAAAAGGAGGGGAAGGCCCCGCCTGCAACCGCGGCAt > 4:280166/1‑275 (MQ=255) gCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGt > 4:375368/1‑275 (MQ=255) gCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGCACCAAATCATAAACGCTGCCCACCGGACAGGTGTTGCCATCGACTAACTTCAGGTTTTTAACGGACACTTGACGCACAAGCACCGGATTGTGTTTTACGCTCCGAAAATGCGGATTTTCAATGCAGCCAAAGTAGGGGAACGCCAACCCTGCAACAGCGTCATGt > 1:99936/1‑275 (MQ=255) aggCC‑GGTAATTGGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTg < 2:299263/273‑1 (MQ=255) cGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTAATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCGGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGa < 2:123601/275‑1 (MQ=255) cc‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGc > 5:218252/1‑59 (MQ=255) tAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCAGCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAg < 2:508/274‑1 (MQ=255) cTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCt < 8:1137079/111‑1 (MQ=255) acACCCCAGCCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCAt < 7:297686/132‑1 (MQ=255) acACCCCAGCCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCAt > 8:297686/2‑133 (MQ=255) cACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAgg > 8:388468/1‑77 (MQ=255) cACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTa > 5:68368/1‑82 (MQ=255) cACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTa < 6:68368/82‑1 (MQ=255) cACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATa < 6:487571/103‑1 (MQ=255) aCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGttt < 8:738684/148‑1 (MQ=255) ccAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCa > 7:1133701/1‑97 (MQ=255) ccAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGgtgt < 8:1128638/122‑1 (MQ=255) ccAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGgtgt > 7:1128638/1‑122 (MQ=255) ccAGCCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaaa < 8:164123/101‑1 (MQ=255) ccAGCCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaaa > 7:164123/1‑101 (MQ=255) aGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAAc > 8:309403/1‑148 (MQ=255) | GCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATAT‑ACTGGCGCGGCACGCC‑GGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTG‑TTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA > NC_000913/1540805‑1541348 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |