| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,797,391 | A→C | 5.3% | L185R (CTG→CGG) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,797,391 | 0 | A | C | 5.3% | 331.3 / 9.7 | 99 | L185R (CTG→CGG) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (54/39); new base C (3/3); total (57/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01 | |||||||||||
ATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGG > NC_000913/1797118‑1797647 | aTGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGT‑CAGGCTTCCGGCGCTTCGACTGTCATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCCGTGGCa > 2:37904/1‑274 (MQ=255) tGCCGCCTTGAAGTCGATCCTTATCGAAGTCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc < 4:421826/275‑1 (MQ=255) tCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGATTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAAACGGAACGATTTCCGGTTGAACCCGCGGCAGCTGGTTCAGCACGGCaa > 2:348154/1‑274 (MQ=255) tATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGCTGGTCGCCCCAACCGGAACGACTTCCGGTTTAACCAGCGCGCCCTGGTTCAACACGGCacactcc > 4:515793/1‑269 (MQ=255) aaGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTGGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCAGTTGAACCAGCGGAAGCTGGTTCAACACGGCAACGtacagccc > 2:162283/1‑267 (MQ=255) cacaTAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGt > 2:373975/1‑275 (MQ=255) cacaTAGTTGGTGACGTCAACAACTGCATCGATAGAACGGACCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGTAGCTGGTTCCGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCCGTCGGCACGGt > 2:46363/1‑275 (MQ=255) gTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTccc > 2:474106/1‑273 (MQ=255) gTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc > 2:523724/1‑275 (MQ=255) gACGTTAAAAACTGCATCGATAGAACGGAACCGGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACg < 2:362996/274‑1 (MQ=255) gACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGCTTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGc > 2:272695/1‑275 (MQ=255) gTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCAGCACGGTTTGGCGTCACGCTGa > 2:314896/1‑275 (MQ=255) caacaaCTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTACTGCCTTTTACCCCACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGCTGGTCGCACCAACCGGAACGATTTCAGGTTGAACCAGCGCCAGCCGGTTCAACACGGCACCGTCACGCCCACCACCAATGATGCCCAAGCACGAGGCACGGGTTGGCCTCCCGCTGAtt > 4:526874/1‑275 (MQ=255) tCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGttt < 2:147383/274‑1 (MQ=255) gCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCCCGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGt > 2:519008/1‑275 (MQ=255) gCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGt > 2:237418/1‑275 (MQ=255) gCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGt > 2:50994/1‑275 (MQ=255) gCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATGTCGATGGAGTTGGCATCAAGTTTCAGGt > 2:443165/1‑275 (MQ=255) aTCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAg > 4:467542/1‑195 (MQ=255) acaGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGt < 2:467543/195‑1 (MQ=255) caGCGGAGTTGGGGTCTTTAA‑TTTACTGCCGTTTAACCAACGGCCAAGCTAA‑GCGGGCCAGGCTGCCGGCGCTCCGACTGTAATCGGCCGCGTGGCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGgcg < 4:237417/275‑1 (MQ=255) caGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGgcg > 1:68081/1‑275 (MQ=255) ggAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCa < 6:673464/148‑1 (MQ=255) gcg‑cTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcagg > 2:182368/1‑274 (MQ=255) tAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 7:1004217/1‑148 (MQ=255) ctgttACCACACGGCCAAGAGAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGGAATCGGCAGCGGGTCGTCGATGGTCGCACAAACCGGAACGATTGCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTGCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcagg < 1:152273/253‑1 (MQ=255) tttACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCtgt > 2:532595/1‑274 (MQ=255) tACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGAt > 2:401204/1‑275 (MQ=255) acacGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaac < 8:811920/117‑3 (MQ=255) acacGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaac > 7:811920/1‑115 (MQ=255) cacGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATAAAGTTTCAGGTATTAACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATACTGCCGGTGGGAACGTCa > 1:67345/1‑275 (MQ=255) acGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCCACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACTCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTTACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTACCGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAg > 2:149238/1‑275 (MQ=255) aaGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc > 5:729937/1‑106 (MQ=255) aaGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc < 6:729937/106‑1 (MQ=255) aaGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGt > 5:1057142/1‑119 (MQ=255) aaGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGt < 6:1057142/119‑1 (MQ=255) tgcaaccgcgGG‑CAGGCTGCCTGCGCTTAGACGGTAATCGGCAGCGTGTCGTCGATTGTCGCACCAACCGGACCGATTTCCGGTTGAACCAGCGGCAGCGGGTTCAGAACGGCAACGTCACGCGCAAACCCAATGATGCCGAATCAGTCGGCACGGTTGGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 4:348153/269‑1 (MQ=255) gATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTc > 6:500037/1‑148 (MQ=255) tAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCgg > 6:532503/1‑148 (MQ=255) cgcgGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTc > 2:273129/1‑275 (MQ=255) ggcgtccgcccGGCGCTTGGACTGTAATCGGCCGCGGTTCGTGGATGGTCGCACCAACCTGAACTATGTCCGGTGGAACCAGCGGCAGCTTGTGCAGAACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTGTGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATAGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCagag < 4:443164/266‑1 (MQ=255) g‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCagag > 2:50118/1‑274 (MQ=255) cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt > 6:36859/1‑88 (MQ=255) cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGtt < 5:36859/88‑1 (MQ=255) cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCa > 5:721272/1‑93 (MQ=255) cTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCa < 6:721272/93‑1 (MQ=255) ttCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgatg > 8:325654/1‑121 (MQ=255) ttCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgatg < 7:325654/121‑1 (MQ=255) gcgcTTCGACGTTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGc < 1:35727/275‑1 (MQ=255) gcTTGGACGGTAATCGGCAGCGTGTCGTCGATGGTGGAACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGGACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCa < 4:273128/274‑1 (MQ=255) gcTTCGACTGTAATCGGCAGCGTGTCGTCGCTGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCAGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCa < 4:314895/274‑1 (MQ=255) ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc < 6:415745/79‑1 (MQ=255) ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc > 5:415745/1‑79 (MQ=255) gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCg < 5:532503/148‑1 (MQ=255) gTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGCACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg > 5:728002/1‑148 (MQ=255) tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgt < 6:728002/148‑1 (MQ=255) aaTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACg < 5:459172/133‑1 (MQ=255) aaTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACg > 6:459172/1‑133 (MQ=255) aTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt < 8:1004217/148‑1 (MQ=255) gCGTGTCGTGGAGGGTGGACCAAACCGGAACGATTTCAGGTTGAACCCGGGCCAGTGGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAGGCTGATTTCGATGGGGTTGTAATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCa < 4:474105/275‑1 (MQ=255) cGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAgg > 2:43468/1‑245 (MQ=255) cgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACg > 6:230308/1‑106 (MQ=255) cgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACg < 5:230308/106‑1 (MQ=255) tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa < 5:500037/148‑1 (MQ=255) cgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc > 7:789273/1‑146 (MQ=255) cgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc < 8:789273/146‑1 (MQ=255) gagggTAGCACCAACCGGAACGATTTCCGGGTGAACCGGGGGCAGATGGTTCAGCACGGCAACGTGACGGGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTcg < 4:519007/272‑1 (MQ=255) gATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTTAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTcg > 2:18444/1‑275 (MQ=255) gTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa > 8:95893/1‑121 (MQ=255) gTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa < 7:95893/121‑1 (MQ=255) tCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCACGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGCTCAGACAAGGAGCACAGCACCCTTTCAGACGGTTCGCCACGCAGTTTcaccgtt > 4:415031/1‑273 (MQ=255) aCCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACgg > 7:500652/1‑148 (MQ=255) aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca < 8:735639/121‑1 (MQ=255) aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca > 7:735639/1‑121 (MQ=255) aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc > 8:769068/1‑127 (MQ=255) aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc < 7:769068/127‑1 (MQ=255) gagttCCGGGTGAACCAGCGGCAGATGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCa < 4:182367/271‑1 (MQ=255) gATTTCCGGATTACCCAGCGGCAGCTGGTTCAGCCCGGCACCGGCACGCGCAACACCAATGAGGCCTAAGCGGGCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGGTGGCATCAAGTTTCAGGTAGTGACGGATATCGGTGACAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCGCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAAGTTTGAAATCa < 4:532594/274‑1 (MQ=255) tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTGAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGNTGTTGTCATCAAGTTTCAGGTATNNACGGATATNGGTGCCAATCGGCGCATCCgcag > 3:31973/1‑162 (MQ=255) tCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCgg > 2:384331/1‑275 (MQ=255) ccGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCgg < 8:500652/148‑1 (MQ=255) cGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTGAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggcag < 1:31973/162‑1 (MQ=255) ttGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCa > 7:1118564/1‑148 (MQ=255) aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa < 6:311960/92‑1 (MQ=255) aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa > 5:311960/1‑92 (MQ=255) aCCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTc > 5:411764/1‑119 (MQ=255) aCCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTc < 6:411764/119‑1 (MQ=255) aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc > 7:151296/1‑111 (MQ=255) aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc < 8:151296/111‑1 (MQ=255) aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCgg > 7:393891/1‑130 (MQ=255) aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCgg < 8:393891/130‑1 (MQ=255) cAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt < 5:1062816/95‑1 (MQ=255) cAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt > 6:1062816/1‑95 (MQ=255) aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt > 6:815082/1‑128 (MQ=255) aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt < 5:815082/128‑1 (MQ=255) gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcagg > 5:754909/1‑148 (MQ=255) cGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggc > 5:235171/1‑148 (MQ=255) cGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggc > 7:1092165/1‑148 (MQ=255) cAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcat > 7:946543/1‑95 (MQ=255) cAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcat < 8:946543/95‑1 (MQ=255) | ATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCG‑CTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGG‑CAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGG > NC_000913/1797118‑1797647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |